BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0096.0020.p (442 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_771472.1| MtbA protein [Bradyrhizobium japonicum USDA 110... 824 0.0 ref|YP_576060.1| general substrate transporter [Nitrobacter hamb... 808 0.0 ref|NP_435971.1| MtbA protein [Sinorhizobium meliloti 1021] >gi|... 787 0.0 ref|YP_004551553.1| major facilitator superfamily protein [Sinor... 765 0.0 ref|YP_002824336.1| major facilitator family transporter [Sinorh... 471 e-131 >ref|NP_771472.1| MtbA protein [Bradyrhizobium japonicum USDA 110] dbj|BAC50097.1| blr4832 [Bradyrhizobium japonicum USDA 110] Length = 442 Score = 824 bits (2128), Expect = 0.0, Method: Compositional matrix adjust. Identities = 412/441 (93%), Positives = 425/441 (96%) Query: 1 MAIQVPSDFRRVIVAASVGNIIEWYDFYIFGSLAAVLSVKFFEQSHPVAALLSTIALFTA 60 MA+QVP DFRRVIVAASVGN+IEWYDFYIFGSLAAVLSVKFFEQSHPVAALLSTIALFTA Sbjct: 1 MAVQVPHDFRRVIVAASVGNVIEWYDFYIFGSLAAVLSVKFFEQSHPVAALLSTIALFTA 60 Query: 61 GFLIRPLGAFLFGWMGDRVGRKYTFLITLSGMGLGTGAIGLIPTYQSIGLTAAFILFGLR 120 GFLIRPLGAFLFGWMGDRVGRKYTFLITLSGMGLGTGAIGLIPTYQSIGLTAAF+LFGLR Sbjct: 61 GFLIRPLGAFLFGWMGDRVGRKYTFLITLSGMGLGTGAIGLIPTYQSIGLTAAFVLFGLR 120 Query: 121 MIQGLCLGGEYGGAITYVAEHVPDHSRGYYTGWLQTSPTLGIVVSLAVIVLTRTWAGNQA 180 MIQGLCLGGEYGGAITYVAEHVPD RGYYTGWLQTSPTLGIVVSLAVIVLTRTW GNQ Sbjct: 121 MIQGLCLGGEYGGAITYVAEHVPDDKRGYYTGWLQTSPTLGIVVSLAVIVLTRTWFGNQV 180 Query: 181 FDEWAWRVPFLVSFLLVAIAIYIRLQLQETPIFQEIKARGQMTRNPWREAFLSANIKYIL 240 FDEWAWRVPFLVSFLLVAIAIYIRLQLQETPIFQEI+ARGQMT NPW+EAFLS+NIKYI Sbjct: 181 FDEWAWRVPFLVSFLLVAIAIYIRLQLQETPIFQEIRARGQMTTNPWKEAFLSSNIKYIG 240 Query: 241 IAIVVLIGQGVVWYSGQFWALYFLQQVSKVDAVTSAYIVGAALLIGTPSLIFFGWLSDQI 300 IAIVVLIGQGVVWYSGQFWALYFLQQVSKVDA+TSAYIVGAALLI TPSLIFFGWLSDQI Sbjct: 241 IAIVVLIGQGVVWYSGQFWALYFLQQVSKVDALTSAYIVGAALLIATPSLIFFGWLSDQI 300 Query: 301 GRKSVILGGMLLAAITYYPLYLWLGMVTQPGHINYPVAVFIIFILVCYVGMVYGPVGAFL 360 GRK VILGGMLLAAITYYPLYLWLG VTQPG+INYP A+FIIFILVCYVGMVYGPVGAFL Sbjct: 301 GRKPVILGGMLLAAITYYPLYLWLGTVTQPGNINYPTAIFIIFILVCYVGMVYGPVGAFL 360 Query: 361 AEFFPARIRYTSVSVPYHIGNGWGGGLVPFITSAAFAATGSIGYALIYPIVVPAVCFVIA 420 AEFFPARIRYTSVSVPYHIGNGWGGGLVPFITSAAFAATGSIGYALIYPI VPAVCFV+A Sbjct: 361 AEFFPARIRYTSVSVPYHIGNGWGGGLVPFITSAAFAATGSIGYALIYPIAVPAVCFVLA 420 Query: 421 VFIMPETRKTRIWEQIEAKAA 441 VF+MPETRK IW+ IE +AA Sbjct: 421 VFLMPETRKISIWQPIEPRAA 441 >ref|YP_576060.1| general substrate transporter [Nitrobacter hamburgensis X14] gb|ABE61600.1| General substrate transporter [Nitrobacter hamburgensis X14] Length = 442 Score = 808 bits (2087), Expect = 0.0, Method: Compositional matrix adjust. Identities = 400/442 (90%), Positives = 423/442 (95%) Query: 1 MAIQVPSDFRRVIVAASVGNIIEWYDFYIFGSLAAVLSVKFFEQSHPVAALLSTIALFTA 60 MAIQVP DFRRVIVAASVGN+IEWYDFYIFGSLAAVLSVKFFEQSHPVAALLSTIALFTA Sbjct: 1 MAIQVPRDFRRVIVAASVGNVIEWYDFYIFGSLAAVLSVKFFEQSHPVAALLSTIALFTA 60 Query: 61 GFLIRPLGAFLFGWMGDRVGRKYTFLITLSGMGLGTGAIGLIPTYQSIGLTAAFILFGLR 120 GFLIRPLGAFLFGWMGDR+GRKYTFL+TLSGMGLGTGAIGLIPTY+SIGLTAAF+LFGLR Sbjct: 61 GFLIRPLGAFLFGWMGDRIGRKYTFLVTLSGMGLGTGAIGLIPTYESIGLTAAFLLFGLR 120 Query: 121 MIQGLCLGGEYGGAITYVAEHVPDHSRGYYTGWLQTSPTLGIVVSLAVIVLTRTWAGNQA 180 MIQGLCLGGEYGGAITYVAEHVPD SRGYYTGWLQTSPTLGIVVSLAVI+ TRT+ GN+A Sbjct: 121 MIQGLCLGGEYGGAITYVAEHVPDESRGYYTGWLQTSPTLGIVVSLAVIIATRTYFGNEA 180 Query: 181 FDEWAWRVPFLVSFLLVAIAIYIRLQLQETPIFQEIKARGQMTRNPWREAFLSANIKYIL 240 F+ WAWRVPFLVSFLLVAIAIYIRLQLQETPIF EIKARGQMTRNPW+EAFLS+N+KY+ Sbjct: 181 FNAWAWRVPFLVSFLLVAIAIYIRLQLQETPIFAEIKARGQMTRNPWKEAFLSSNVKYVG 240 Query: 241 IAIVVLIGQGVVWYSGQFWALYFLQQVSKVDAVTSAYIVGAALLIGTPSLIFFGWLSDQI 300 IA +VLIGQGVVWYSGQFWALYFLQQVSKVDA+TSAYIVGAALLI TPSLIFFGWLSD I Sbjct: 241 IATIVLIGQGVVWYSGQFWALYFLQQVSKVDALTSAYIVGAALLIATPSLIFFGWLSDVI 300 Query: 301 GRKSVILGGMLLAAITYYPLYLWLGMVTQPGHINYPVAVFIIFILVCYVGMVYGPVGAFL 360 GRK VILGGMLLAAITYYPLYLWLG VTQPG+INYP+AVFIIFILVCYVGMVYGPVGAFL Sbjct: 301 GRKPVILGGMLLAAITYYPLYLWLGTVTQPGNINYPIAVFIIFILVCYVGMVYGPVGAFL 360 Query: 361 AEFFPARIRYTSVSVPYHIGNGWGGGLVPFITSAAFAATGSIGYALIYPIVVPAVCFVIA 420 AE+FP RIRYTSVSVPYHIGNGWGGGLVPFITSAAFAATG+IGYALIYPI VPAVCFV+A Sbjct: 361 AEYFPGRIRYTSVSVPYHIGNGWGGGLVPFITSAAFAATGNIGYALIYPIAVPAVCFVLA 420 Query: 421 VFIMPETRKTRIWEQIEAKAAS 442 +F+MPETRK IWE IE + AS Sbjct: 421 LFLMPETRKISIWEPIEPRTAS 442 >ref|NP_435971.1| MtbA protein [Sinorhizobium meliloti 1021] gb|AAK65383.1| Permease [Sinorhizobium meliloti 1021] gb|AEG07356.1| General substrate transporter [Sinorhizobium meliloti BL225C] gb|AEH82006.1| Permease [Sinorhizobium meliloti SM11] Length = 440 Score = 787 bits (2032), Expect = 0.0, Method: Compositional matrix adjust. Identities = 388/439 (88%), Positives = 415/439 (94%) Query: 1 MAIQVPSDFRRVIVAASVGNIIEWYDFYIFGSLAAVLSVKFFEQSHPVAALLSTIALFTA 60 MA+QVP+DFRRVIVAASVGNIIEWYDFYIFGSLAAVLSVKFFEQSHPVAALLSTIALFTA Sbjct: 1 MAVQVPTDFRRVIVAASVGNIIEWYDFYIFGSLAAVLSVKFFEQSHPVAALLSTIALFTA 60 Query: 61 GFLIRPLGAFLFGWMGDRVGRKYTFLITLSGMGLGTGAIGLIPTYQSIGLTAAFILFGLR 120 GFLIRPLGAFLFGWMGDRVGRKYTFLITL+GMGLGTGAIGLIPTY+SIGLTAAF+LF LR Sbjct: 61 GFLIRPLGAFLFGWMGDRVGRKYTFLITLTGMGLGTGAIGLIPTYESIGLTAAFLLFSLR 120 Query: 121 MIQGLCLGGEYGGAITYVAEHVPDHSRGYYTGWLQTSPTLGIVVSLAVIVLTRTWAGNQA 180 MIQGLCLGGEYGGAITYVAEHVPD RGYYTGWLQTSPTLGIVVSLAVI+ RT+ G++A Sbjct: 121 MIQGLCLGGEYGGAITYVAEHVPDERRGYYTGWLQTSPTLGIVVSLAVIIAARTYFGSEA 180 Query: 181 FDEWAWRVPFLVSFLLVAIAIYIRLQLQETPIFQEIKARGQMTRNPWREAFLSANIKYIL 240 FD WAWRVPFLVSFLLV IAIYIRLQLQETPIFQEIKA+GQMT+NPWREAFLS+NIKY+ Sbjct: 181 FDAWAWRVPFLVSFLLVGIAIYIRLQLQETPIFQEIKAKGQMTQNPWREAFLSSNIKYVG 240 Query: 241 IAIVVLIGQGVVWYSGQFWALYFLQQVSKVDAVTSAYIVGAALLIGTPSLIFFGWLSDQI 300 IA +VLIGQGVVWYSGQFWALYFLQQVSKVD + SAYIVGAALL+ TPSLI FGWLSD I Sbjct: 241 IATIVLIGQGVVWYSGQFWALYFLQQVSKVDPLNSAYIVGAALLLATPSLILFGWLSDII 300 Query: 301 GRKSVILGGMLLAAITYYPLYLWLGMVTQPGHINYPVAVFIIFILVCYVGMVYGPVGAFL 360 GRK VILGGMLLAA+TYYPLYLWLG VTQP +INYP+A+FIIFILVCYVGMVYGPVGAFL Sbjct: 301 GRKPVILGGMLLAALTYYPLYLWLGAVTQPDNINYPIAIFIIFILVCYVGMVYGPVGAFL 360 Query: 361 AEFFPARIRYTSVSVPYHIGNGWGGGLVPFITSAAFAATGSIGYALIYPIVVPAVCFVIA 420 AE+FP RIRYTSVSVPYHIGNGWGGGLVPFITSAAFAATGSIGYALIYPI VPAVCFV+A Sbjct: 361 AEYFPGRIRYTSVSVPYHIGNGWGGGLVPFITSAAFAATGSIGYALIYPIAVPAVCFVLA 420 Query: 421 VFIMPETRKTRIWEQIEAK 439 +F+MPETR+ IW+ IE + Sbjct: 421 IFLMPETRRISIWQPIEPR 439 >ref|YP_004551553.1| major facilitator superfamily protein [Sinorhizobium meliloti AK83] gb|AEG57430.1| major facilitator superfamily MFS_1 [Sinorhizobium meliloti AK83] Length = 461 Score = 765 bits (1975), Expect = 0.0, Method: Compositional matrix adjust. Identities = 383/442 (86%), Positives = 410/442 (92%), Gaps = 3/442 (0%) Query: 1 MAIQVPSDFRRVIVAASVGNIIEWYDFYIFGSLAAVLSVKFFEQSHPVAALLSTIALFTA 60 MA+QVP+DFRRVIVAASVGNIIEWYDFY+FGSLAAVLSVKFFEQSHPVAALLSTIALFTA Sbjct: 1 MAVQVPTDFRRVIVAASVGNIIEWYDFYVFGSLAAVLSVKFFEQSHPVAALLSTIALFTA 60 Query: 61 GFLIRPLGAFLFGWMGDRVGRKYTFLITLSGMGLGTGAIGLIPTYQSIGLTAAFILFGLR 120 GFLIRPLGAFLFGWMGDRVGRKYTFLITL+GMGLGTGAIGLIPTY+SIGLTAAF+LF LR Sbjct: 61 GFLIRPLGAFLFGWMGDRVGRKYTFLITLTGMGLGTGAIGLIPTYESIGLTAAFLLFSLR 120 Query: 121 MIQGLCLGGEYGGAITYVAEHVPDHSRGYYTGWLQTSPTLGIVVSLAVIVLTRTWAGNQA 180 MIQGLCLGGEYGGAITYVAEHVPD RGYYTGWLQTSPTLGIVVSLAVI+ RT+ G++A Sbjct: 121 MIQGLCLGGEYGGAITYVAEHVPDERRGYYTGWLQTSPTLGIVVSLAVIIAARTYFGSEA 180 Query: 181 FDEWAWRVPFLVSFLLVAIAIYIRLQLQETPIFQEIKARGQMTRNPWREAFLSANIKYIL 240 FD WAWRVPFLVSFLLV IAIYIRLQLQETPIFQEIKA+GQMT+NPWREAFLS+NIKY+ Sbjct: 181 FDAWAWRVPFLVSFLLVGIAIYIRLQLQETPIFQEIKAKGQMTQNPWREAFLSSNIKYVG 240 Query: 241 IAIVVLIGQGVVWYSGQFWALYFLQQVSKVDAVTSAYIVGAALLIGTPSLIFFGWLSDQI 300 IA +VLIGQGVVWYSGQFWALYFLQQVSKVD + SAYIVGAALL+ TPSLI FGWLSD I Sbjct: 241 IATIVLIGQGVVWYSGQFWALYFLQQVSKVDPLNSAYIVGAALLLATPSLILFGWLSDII 300 Query: 301 GRKSVILGGMLLAAITYYPLYLWLGMVTQPGHINYPVAVFIIFILVCYVGMVYGPVGAFL 360 GRK VILGGMLLAA+TYYPLYLWLG VTQP +INYP+A+FIIFILVCYVGMVYGPVGAFL Sbjct: 301 GRKPVILGGMLLAALTYYPLYLWLGAVTQPDNINYPIAIFIIFILVCYVGMVYGPVGAFL 360 Query: 361 AEFFPARIRYTSVSVPYHIGNGWGGGLVPFITSAAFAATGSIGYALIYPIVVPAVCFVIA 420 AE+FP RIRYTSVSVPYHIGNGWGGGLVPFITSAAFAATGSIGYALIYPI VPAVCFV+A Sbjct: 361 AEYFPGRIRYTSVSVPYHIGNGWGGGLVPFITSAAFAATGSIGYALIYPIAVPAVCFVLA 420 Query: 421 VFIMPETRKTRIWEQIEAKAAS 442 +F P R + + + A AS Sbjct: 421 IF--PNARDPQN-KHLAANRAS 439 >ref|YP_002824336.1| major facilitator family transporter [Sinorhizobium fredii NGR234] gb|ACP23583.1| major facilitator family transporter [Sinorhizobium fredii NGR234] Length = 275 Score = 471 bits (1213), Expect = e-131, Method: Compositional matrix adjust. Identities = 228/275 (82%), Positives = 254/275 (92%) Query: 168 VIVLTRTWAGNQAFDEWAWRVPFLVSFLLVAIAIYIRLQLQETPIFQEIKARGQMTRNPW 227 +I+ TRT+ G + FD WAWRVPFLVSF+LVAIAIYIRLQLQETPIF+EIKA+GQMTRNPW Sbjct: 1 MIIATRTYFGTEVFDAWAWRVPFLVSFVLVAIAIYIRLQLQETPIFEEIKAKGQMTRNPW 60 Query: 228 REAFLSANIKYILIAIVVLIGQGVVWYSGQFWALYFLQQVSKVDAVTSAYIVGAALLIGT 287 REAFLS+NIKY+ IA +VLIGQGVVWYSGQFWALYFLQQVSKVDA+TS+YIVGAALL+ T Sbjct: 61 REAFLSSNIKYVGIATIVLIGQGVVWYSGQFWALYFLQQVSKVDALTSSYIVGAALLLAT 120 Query: 288 PSLIFFGWLSDQIGRKSVILGGMLLAAITYYPLYLWLGMVTQPGHINYPVAVFIIFILVC 347 PSLIFFGWLSD IGRK VILGGMLLAA TYYPLYLWLG VTQP ++NYPVA+FIIFILVC Sbjct: 121 PSLIFFGWLSDIIGRKPVILGGMLLAATTYYPLYLWLGTVTQPDNVNYPVAIFIIFILVC 180 Query: 348 YVGMVYGPVGAFLAEFFPARIRYTSVSVPYHIGNGWGGGLVPFITSAAFAATGSIGYALI 407 YVGMVYGPVGAFLAEFFP RIRYTSVSVPYHIGNGWGGGLVPF+TSAAFAATGS+GYALI Sbjct: 181 YVGMVYGPVGAFLAEFFPGRIRYTSVSVPYHIGNGWGGGLVPFVTSAAFAATGSVGYALI 240 Query: 408 YPIVVPAVCFVIAVFIMPETRKTRIWEQIEAKAAS 442 YPI VPAVCF++A+++MPETR+ IWE +E + S Sbjct: 241 YPIAVPAVCFLLALWLMPETRRVSIWEPVEPRLRS 275