BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0087.0035.p (596 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_767686.1| feruloyl-CoA synthase [Bradyrhizobium japonicum... 890 0.0 ref|ZP_08628166.1| long-chain-fatty-acid--CoA ligase [Bradyrhizo... 780 0.0 ref|YP_001242742.1| feruloyl-CoA synthase [Bradyrhizobium sp. BT... 769 0.0 ref|YP_001203249.1| feruloyl-CoA synthase [Bradyrhizobium sp. OR... 753 0.0 ref|YP_160047.1| feruloyl-CoA synthase [Aromatoleum aromaticum E... 659 0.0 >ref|NP_767686.1| feruloyl-CoA synthase [Bradyrhizobium japonicum USDA 110] dbj|BAC46311.1| long-chain-fatty-acid-CoA ligase [Bradyrhizobium japonicum USDA 110] Length = 644 Score = 890 bits (2301), Expect = 0.0, Method: Compositional matrix adjust. Identities = 441/541 (81%), Positives = 473/541 (87%) Query: 3 MAAASGDNPSSLFATPAIVAASRNDGSILVRSTTPLKQGARCVGDWLEHWARLAPDRIFL 62 M AA + SSLFA P +A R DGSI++RS PL ARC+GDWLE WAR PD IFL Sbjct: 27 MTAALRGDASSLFAAPKTIAEHRADGSIVLRSPEPLHDSARCIGDWLEQWARQTPDAIFL 86 Query: 63 GERANAEVPWSTVSYRDALGIVRRAASWVLAQGLSAERPLVILSDNGIDHALFALAAQHV 122 ER NAE PW+TV+Y AL VR AASW+L+QGLSAERPLVILSDN IDHAL ALAAQHV Sbjct: 87 AERGNAEAPWTTVTYAQALRQVRGAASWILSQGLSAERPLVILSDNSIDHALLALAAQHV 146 Query: 123 GVPSAAISPAYSLMSRDFDKLKGMIELLDPGAIYATSTGPFAAALAAIKPLHRATIVSGN 182 GVPSAAISPAYSLMSRDFDKLK MI LL+PGAIY ++T PFAAALAAI+PLH+A I+SGN Sbjct: 147 GVPSAAISPAYSLMSRDFDKLKSMIALLEPGAIYVSATKPFAAALAAIEPLHKAQIISGN 206 Query: 183 PDDADALPFRAVAATPEALEVAKAFAAVTADTIAKFLFTSGSTGTPKAVINTQRMLTSSQ 242 DADAL F AVAATPE +VAKAFAAVT DTIAKFLFTSGSTGTPKAVINTQRMLTSSQ Sbjct: 207 GADADALAFSAVAATPEIADVAKAFAAVTPDTIAKFLFTSGSTGTPKAVINTQRMLTSSQ 266 Query: 243 QAKAQTWTFLEQSRDDLVILDWLPWSHTFGANHNFNLVLRNGGTLYIDGGKPAPGLFATS 302 QAKAQTWTFLEQ+ DLVILDWLPWSHTFGANHNFNLVLRNGG+LYIDGGKPAPGLFATS Sbjct: 267 QAKAQTWTFLEQAGSDLVILDWLPWSHTFGANHNFNLVLRNGGSLYIDGGKPAPGLFATS 326 Query: 303 LANLRSVVPTVYFNVPRGFDMLIAALRTDDELRKKFFEGTKFAFYAGAALPQNLWDALED 362 LANL+SV+PTVYFNVPRGFDMLIAALR D+ LR++FF KFAFYAGAALPQNLWDALE Sbjct: 327 LANLKSVMPTVYFNVPRGFDMLIAALRGDEALRRRFFSEVKFAFYAGAALPQNLWDALEQ 386 Query: 363 LSLRTVGRRLPMVSAWGSTETSPLATDCHFLAKRSGNIGVPIPGTELKLVPSGDKLEVRV 422 LS+ TVGR LPMVSAWGSTETSPLATDCHFLA+RSGNIGVPIPGTELKLV SGDKLEVRV Sbjct: 387 LSVETVGRPLPMVSAWGSTETSPLATDCHFLAERSGNIGVPIPGTELKLVTSGDKLEVRV 446 Query: 423 RGPNVTPGYWKAPELTAEAFDDEGFYKIGDAVTFADPAWPDLGLFFDGRVAEDFKLNSGT 482 RGPNVTPGYWKAPELT +AFDDEGFY IGDAV AD A P+ GLFFDGRVAEDFKLNSGT Sbjct: 447 RGPNVTPGYWKAPELTRQAFDDEGFYLIGDAVKLADSARPERGLFFDGRVAEDFKLNSGT 506 Query: 483 WVSVGTLRVAGIAALAPLAQDIVVTGHGRDEVRFLVFPNLAACRSLAGLPESADAREVIG 542 WVSVGTLR+AGIAALAPLAQDIVV GHG DEVRFLVFPN+ ACR AGLPE+AD +V+ Sbjct: 507 WVSVGTLRLAGIAALAPLAQDIVVAGHGGDEVRFLVFPNILACRGHAGLPETADVNDVLA 566 Query: 543 H 543 H Sbjct: 567 H 567 >ref|ZP_08628166.1| long-chain-fatty-acid--CoA ligase [Bradyrhizobiaceae bacterium SG-6C] gb|EGP09075.1| long-chain-fatty-acid--CoA ligase [Bradyrhizobiaceae bacterium SG-6C] Length = 619 Score = 780 bits (2014), Expect = 0.0, Method: Compositional matrix adjust. Identities = 384/533 (72%), Positives = 441/533 (82%), Gaps = 4/533 (0%) Query: 14 LFATPAIVAASRNDGSILVRSTTPLKQGARCVGDWLEHWARLAPDRIFLGERANAEVPWS 73 LFA P I+A + DGSI ++S TPL+ ARCVGDWLE WAR PD +FL ERA+A++PW Sbjct: 15 LFAAPNIIADRKADGSIWLKSATPLQPFARCVGDWLEQWARETPDTVFLAERASADLPWR 74 Query: 74 TVSYRDALGIVRRAASWVLAQGLSAERPLVILSDNGIDHALFALAAQHVGVPSAAISPAY 133 ++YR+ + VR ASW+LAQG+SAERPLVILSDN IDHA+F LAA HVGVP+A +SPAY Sbjct: 75 KLTYRETMRRVRAVASWILAQGMSAERPLVILSDNSIDHAIFMLAAMHVGVPAAPVSPAY 134 Query: 134 SLMSRDFDKLKGMIELLDPGAIYATSTGPFAAALAAIKPLHRATIVSGNPDDAD-ALPFR 192 SLMS+D+DKLK MI LLDPGAIY +S FA AL AI P H A +V+G+PD + A+ F Sbjct: 135 SLMSKDYDKLKSMIALLDPGAIYVSSATMFATALNAIAPAHTAIVVAGDPDGFEGAIAFS 194 Query: 193 AVAATPEALEVAKAFAAVTADTIAKFLFTSGSTGTPKAVINTQRMLTSSQQAKAQTWTFL 252 ++ A E +VA+AFA V DTIAKFLFTSGSTG PKAVINTQRMLTS+Q AK QTW FL Sbjct: 195 SLTAAAETPDVARAFAGVGHDTIAKFLFTSGSTGAPKAVINTQRMLTSNQAAKGQTWPFL 254 Query: 253 EQSRDDLVILDWLPWSHTFGANHNFNLVLRNGGTLYIDGGKPAPGLFATSLANLRSVVPT 312 ++ LDWLPWSHTFGANHNFN VLRNGGTLYID GKPAPGLFA SLANLR V+PT Sbjct: 255 SET--GFTTLDWLPWSHTFGANHNFNCVLRNGGTLYIDSGKPAPGLFALSLANLRDVIPT 312 Query: 313 VYFNVPRGFDMLIAALRTDDELRKKFFEGTKFAFYAGAALPQNLWDALEDLSLRTVGRRL 372 VYFNVPRG+DMLIAALR DD+LR++FF K FYA AALPQNLWDALE LS+ TVG + Sbjct: 313 VYFNVPRGYDMLIAALREDDDLRRRFFSDVKLLFYAAAALPQNLWDALEALSIATVGHAV 372 Query: 373 PMVSAWGSTETSPLATDCHFLAKRSGNIGVPIPGTELKLVPSGDKLEVRVRGPNVTPGYW 432 PMVSAWGSTET+PLATDCHFLA+RSGNIGVP+PGTE+KLVP+GDKLE RVRGPNVTPGYW Sbjct: 373 PMVSAWGSTETAPLATDCHFLAQRSGNIGVPVPGTEIKLVPTGDKLEARVRGPNVTPGYW 432 Query: 433 KAPELTAEAFDDEGFYKIGDAVTFADPAWPDLGLFFDGRVAEDFKLNSGTWVSVGTLRVA 492 KAPE TA+AFD +GFY +GDA+ AD A P+LGLFFDGRVAEDFKL SGTWVSVGTLR+A Sbjct: 433 KAPEQTAKAFDADGFYMMGDAMVLADSARPELGLFFDGRVAEDFKLTSGTWVSVGTLRIA 492 Query: 493 GIAALAPLAQDIVVTGHGRDEVRFLVFPNLAACRSLAGLPESADAREVIGHPA 545 GIAALAP+AQDIVVTGH RDEVRFLVFPN+AACR+LA LP+ AD +V+ PA Sbjct: 493 GIAALAPIAQDIVVTGHDRDEVRFLVFPNIAACRALAKLPD-ADVADVLRAPA 544 >ref|YP_001242742.1| feruloyl-CoA synthase [Bradyrhizobium sp. BTAi1] gb|ABQ38836.1| trans-feruloyl-CoA synthase [Bradyrhizobium sp. BTAi1] Length = 617 Score = 769 bits (1985), Expect = 0.0, Method: Compositional matrix adjust. Identities = 374/534 (70%), Positives = 434/534 (81%), Gaps = 2/534 (0%) Query: 12 SSLFATPAIVAASRNDGSILVRSTTPLKQGARCVGDWLEHWARLAPDRIFLGERANAEVP 71 +S+FA PAI A R+DG+I++RS TPL ARC G+WLEHWAR PDR FL ER Sbjct: 12 NSMFAEPAIDAVRRDDGAIILRSRTPLAPAARCSGEWLEHWARSVPDRTFLAERRGDG-- 69 Query: 72 WSTVSYRDALGIVRRAASWVLAQGLSAERPLVILSDNGIDHALFALAAQHVGVPSAAISP 131 W+ ++Y + VR AA+W+LAQ L +RPL ILSDN I HA+ ALAAQHVGVP +AISP Sbjct: 70 WTGLTYGETRAQVRAAAAWMLAQSLGPDRPLAILSDNSIAHAVLALAAQHVGVPVSAISP 129 Query: 132 AYSLMSRDFDKLKGMIELLDPGAIYATSTGPFAAALAAIKPLHRATIVSGNPDDADALPF 191 AYSLMS D +KL+ MI LLDPGAI+ PFA AL+AI+ LHRATI++G+ D A+P Sbjct: 130 AYSLMSTDHEKLRAMIRLLDPGAIFVAQRAPFAPALSAIESLHRATIIAGDGDGTSAVPL 189 Query: 192 RAVAATPEALEVAKAFAAVTADTIAKFLFTSGSTGTPKAVINTQRMLTSSQQAKAQTWTF 251 +++ AT EV +AFAAVT DT+AK LFTSGSTG PKAVINTQRM+T+SQQAKAQ WTF Sbjct: 190 QSLIATTPGPEVDRAFAAVTPDTVAKLLFTSGSTGQPKAVINTQRMMTTSQQAKAQLWTF 249 Query: 252 LEQSRDDLVILDWLPWSHTFGANHNFNLVLRNGGTLYIDGGKPAPGLFATSLANLRSVVP 311 L + VI+DWLPWSHTFGANHNFNLVL GG+LYIDGGKPAPGLF TSLANLRS VP Sbjct: 250 LREVEGGPVIVDWLPWSHTFGANHNFNLVLSQGGSLYIDGGKPAPGLFDTSLANLRSKVP 309 Query: 312 TVYFNVPRGFDMLIAALRTDDELRKKFFEGTKFAFYAGAALPQNLWDALEDLSLRTVGRR 371 T+YFNVPRGFDML+AAL+TDD+LR++FF G + FYA AALPQNLWDAL ++SL +VGR Sbjct: 310 TIYFNVPRGFDMLVAALKTDDDLRQQFFSGVRCIFYAAAALPQNLWDALNEMSLASVGRP 369 Query: 372 LPMVSAWGSTETSPLATDCHFLAKRSGNIGVPIPGTELKLVPSGDKLEVRVRGPNVTPGY 431 P++SAWG+TETSPLATDCHF A+R+GNIGVPIPGTELKLVPSG+KLE+RVRGPNVTPGY Sbjct: 370 APLLSAWGATETSPLATDCHFFAERAGNIGVPIPGTELKLVPSGEKLEIRVRGPNVTPGY 429 Query: 432 WKAPELTAEAFDDEGFYKIGDAVTFADPAWPDLGLFFDGRVAEDFKLNSGTWVSVGTLRV 491 WKAPELTA AFDDEGFYKIGDAV FADP P+ GL FDGR+ EDFKL+SGTWV+VG LR+ Sbjct: 430 WKAPELTAAAFDDEGFYKIGDAVKFADPEHPERGLVFDGRITEDFKLSSGTWVNVGALRI 489 Query: 492 AGIAALAPLAQDIVVTGHGRDEVRFLVFPNLAACRSLAGLPESADAREVIGHPA 545 AGIAALAPLAQDIVV GHG DEV FLV PN+AACRS A L +A EV+ HPA Sbjct: 490 AGIAALAPLAQDIVVAGHGTDEVHFLVVPNIAACRSKAALANTASIGEVLAHPA 543 >ref|YP_001203249.1| feruloyl-CoA synthase [Bradyrhizobium sp. ORS 278] emb|CAL75012.1| Long-chain-fatty-acid-CoA ligase (Long-chain acyl-CoA synthetase) (Acyl-CoA synthetase) [Bradyrhizobium sp. ORS 278] Length = 620 Score = 753 bits (1944), Expect = 0.0, Method: Compositional matrix adjust. Identities = 367/537 (68%), Positives = 427/537 (79%), Gaps = 2/537 (0%) Query: 9 DNPSSLFATPAIVAASRNDGSILVRSTTPLKQGARCVGDWLEHWARLAPDRIFLGERANA 68 D +FA P+I A R+DG+I++RS PL+ ARC G+WLE WAR APDR FL ER Sbjct: 9 DPAPEMFAAPSIDALKRDDGAIILRSRMPLEPSARCTGEWLETWARKAPDRTFLAERRGD 68 Query: 69 EVPWSTVSYRDALGIVRRAASWVLAQGLSAERPLVILSDNGIDHALFALAAQHVGVPSAA 128 W+ ++Y + L VR AA+W+LAQGL ERPL ILSDN I HA+ ALAAQHVG+P A Sbjct: 69 T--WTWLTYGETLTQVRAAAAWMLAQGLGPERPLAILSDNSIAHAVLALAAQHVGIPVCA 126 Query: 129 ISPAYSLMSRDFDKLKGMIELLDPGAIYATSTGPFAAALAAIKPLHRATIVSGNPDDADA 188 IS AYSLMS D +KL+ MI LLDPGAI+ + GPF ALAAI+ LHRA I+SG+ + + A Sbjct: 127 ISSAYSLMSTDHEKLRTMIRLLDPGAIFVAAHGPFVRALAAIEGLHRAVIISGDGEGSGA 186 Query: 189 LPFRAVAATPEALEVAKAFAAVTADTIAKFLFTSGSTGTPKAVINTQRMLTSSQQAKAQT 248 +P +A+ T EV +AFAAVT DTIAK LFTSGSTG PKAVINT MLT SQQAK Q Sbjct: 187 MPLQALLTTKSGHEVDRAFAAVTPDTIAKLLFTSGSTGQPKAVINTHGMLTMSQQAKGQL 246 Query: 249 WTFLEQSRDDLVILDWLPWSHTFGANHNFNLVLRNGGTLYIDGGKPAPGLFATSLANLRS 308 WTFL + VI+DWLPWSHTFGANHNFNLVL +GG++YIDGGKPAPGLF TSLANLRS Sbjct: 247 WTFLREVEGGPVIVDWLPWSHTFGANHNFNLVLAHGGSIYIDGGKPAPGLFETSLANLRS 306 Query: 309 VVPTVYFNVPRGFDMLIAALRTDDELRKKFFEGTKFAFYAGAALPQNLWDALEDLSLRTV 368 +VPT+YFNVPRGFDML+AAL+ DDELR KFF G + YA AALPQNLWDAL ++SL TV Sbjct: 307 MVPTIYFNVPRGFDMLVAALKADDELRTKFFSGVQCILYAAAALPQNLWDALNEMSLATV 366 Query: 369 GRRLPMVSAWGSTETSPLATDCHFLAKRSGNIGVPIPGTELKLVPSGDKLEVRVRGPNVT 428 GR+ P+VSAWG+TETSPLATDCHF A+R+GNIGVPIPGTELKLVP+G+KLEVRVRGPNV Sbjct: 367 GRKAPLVSAWGATETSPLATDCHFFAERAGNIGVPIPGTELKLVPAGEKLEVRVRGPNVM 426 Query: 429 PGYWKAPELTAEAFDDEGFYKIGDAVTFADPAWPDLGLFFDGRVAEDFKLNSGTWVSVGT 488 PGYWKAPE+TA AFD+EGFYKIGDAV ADP P+ GLFFDGR+ EDFKL+SGTWV+VG Sbjct: 427 PGYWKAPEITAAAFDEEGFYKIGDAVKLADPDHPEAGLFFDGRITEDFKLSSGTWVNVGA 486 Query: 489 LRVAGIAALAPLAQDIVVTGHGRDEVRFLVFPNLAACRSLAGLPESADAREVIGHPA 545 LRVAGIAALAPLAQDIV+ GHG DEV FL+ PN+ ACRS A L ++ EV+ HPA Sbjct: 487 LRVAGIAALAPLAQDIVIAGHGTDEVHFLIVPNIIACRSKAALADTCSIGEVLAHPA 543 >ref|YP_160047.1| feruloyl-CoA synthase [Aromatoleum aromaticum EbN1] emb|CAI09146.1| Long-chain-fatty-acid-CoA ligase (EC 6.2.1.3) [Aromatoleum aromaticum EbN1] Length = 624 Score = 659 bits (1700), Expect = 0.0, Method: Compositional matrix adjust. Identities = 334/541 (61%), Positives = 395/541 (73%), Gaps = 7/541 (1%) Query: 13 SLFATPAIVAASRNDGSILVRSTTPLKQG-ARCVGDWLEHWARLAPDRIFLGERANAEVP 71 ++FA P + +R DGS ++RS L G ARCVG+WLE WAR P+R+FL ER N Sbjct: 17 AMFARPVVNIEARPDGSRVLRSGIQLPDGYARCVGEWLERWARDTPERLFLAER-NVAGE 75 Query: 72 WSTVSYRDALGIVRRAASWVLAQGLSAERPLVILSDNGIDHALFALAAQHVGVPSAAISP 131 W VSY +A V A+W+L Q LSAERP+ ILSDN I+HAL LAA H+GVPS AIS Sbjct: 76 WDRVSYGEARRRVIAIATWLLGQNLSAERPVAILSDNSIEHALLMLAAMHIGVPSCAISS 135 Query: 132 AYSLMSRDFDKLKGMIELLDPGAIYATSTGPFAAALAAIKPLHRATIVSGNPDDADA--L 189 SLMS+DF KLK IELL PG IYA G FAAA+ AI+PLH +V+G ++ A + Sbjct: 136 GNSLMSKDFAKLKANIELLRPGVIYADQVGKFAAAIDAIRPLHDGVVVAGGGSESTAGCM 195 Query: 190 PFRAVAATPEALEVAKAFAAVTADTIAKFLFTSGSTGTPKAVINTQRMLTSSQQAKAQTW 249 PF A+ A + V +A+A +T DTIAKFLFTSGS GTPKAVINTQRML +SQ AK W Sbjct: 196 PFAAIEADSDEAAVMEAYARITPDTIAKFLFTSGSVGTPKAVINTQRMLCASQLAKELVW 255 Query: 250 TFLEQSRDDLVILDWLPWSHTFGANHNFNLVLRNGGTLYIDGGKPAPGLFATSLANLRSV 309 FL + ++++WLPWSHTFG+NHNFN+VLR GG++Y+D GKP P L +L NLR V Sbjct: 256 PFLLH--EPPLLMEWLPWSHTFGSNHNFNMVLRFGGSMYLDDGKPTPALLDKTLRNLREV 313 Query: 310 VPTVYFNVPRGFDMLIAALRTDDELRKKFFEGTKFAFYAGAALPQNLWDALEDLSLRTVG 369 PT+YF+VPR FDML+ LR D LR FF+ K FYAGAALPQ+LW LE+LS G Sbjct: 314 SPTIYFSVPRAFDMLVPVLRRDKVLRDSFFKRLKLIFYAGAALPQHLWAELENLSEEATG 373 Query: 370 RRLPMVSAWGSTETSPLATDCHFLAKRSGNIGVPIPGTELKLVPSGDKLEVRVRGPNVTP 429 RR+ MVS+WGSTETSPLATDCHF A RSG IGVP+PGTELKLVPS DKLEVRVRGPNV P Sbjct: 374 RRVTMVSSWGSTETSPLATDCHFQAVRSGVIGVPVPGTELKLVPSADKLEVRVRGPNVFP 433 Query: 430 GYWKAPELTAEAFDDEGFYKIGDAVTFADPAWPDLGLFFDGRVAEDFKLNSGTWVSVGTL 489 GYWK PELTA++FDDEGFY IGDAV F DPA P+ GL FDGRV EDFKL +GTWV VG L Sbjct: 434 GYWKQPELTAKSFDDEGFYLIGDAVEFVDPARPEQGLLFDGRVGEDFKLLTGTWVHVGAL 493 Query: 490 RVAGIAALAPLAQDIVVTGHGRDEVRFLVFPNLAACRSLA-GLPESADAREVIGHPASVP 548 RV GI AL P+AQDIVVTGH RD++ FLVFPN+ CRSL GLP A +V+ +PA + Sbjct: 494 RVKGIDALKPVAQDIVVTGHDRDDIGFLVFPNIPECRSLCPGLPSDAPVEQVLSNPAVLA 553 Query: 549 R 549 R Sbjct: 554 R 554