BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0082.0024.p (310 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_002496106.1| alpha/beta hydrolase fold protein [Methyloba... 293 2e-77 ref|YP_001768761.1| alpha/beta hydrolase fold protein [Methyloba... 292 5e-77 ref|YP_001924150.1| alpha/beta hydrolase fold protein [Methyloba... 289 4e-76 ref|YP_001638925.1| alpha/beta hydrolase fold protein [Methyloba... 282 5e-74 ref|YP_002962483.1| alpha/beta hydrolase, epoxide hydrolase [met... 281 8e-74 >ref|YP_002496106.1| alpha/beta hydrolase fold protein [Methylobacterium nodulans ORS 2060] gb|ACL55803.1| alpha/beta hydrolase fold protein [Methylobacterium nodulans ORS 2060] Length = 312 Score = 293 bits (751), Expect = 2e-77, Method: Compositional matrix adjust. Identities = 153/287 (53%), Positives = 201/287 (70%), Gaps = 3/287 (1%) Query: 16 ITQQKIDIGGLRVNVARFGKGAPLLLLHGWPEFWLVWRPLMLRLGDSFELIAPDLRGCGD 75 I + + G+R++VAR G+G PL+LLHGWPEFW W PLM RL F+L+APDLRG G+ Sbjct: 19 IRHGTVAVRGIRMHVARCGEGPPLVLLHGWPEFWATWEPLMARLAGRFDLVAPDLRGFGE 78 Query: 76 TGK-PTPGPDASATAERHALDMFAVMDALGHDRFGVIGGDLGAYVMQAMSHRQPQRLTGT 134 TG+ P PD + AE HA D+ A++DALG R G++G D+GAYVMQA + R P+RL+G Sbjct: 79 TGRDPLAPPDPTVDAEAHAADLLALLDALGLARVGLVGHDVGAYVMQAFARRHPERLSGL 138 Query: 135 LYLCTPYPGLGHRYGQPGHLIEVWYQYFQQLPWAAQLVGSSRESCRLYFRHFLDHWSGDD 194 + P PG+G R+ + GHL E WYQYF Q P+AA LVG+SRESC Y HFL HWS D Sbjct: 139 FFFNCPTPGIGPRWAEAGHLKETWYQYFHQQPFAAALVGASRESCATYIGHFLRHWSA-D 197 Query: 195 PAVFGDLLEAYVDNFMKPGNIQGGFDWYLSSAPNRRRWLEGKLPPQPRIDVPSRFLWGRR 254 P F LL+ +VDNF++PGN+QGGFDWYLS+AP+RR+ +EG P P I VP+R LWG R Sbjct: 198 PETFAPLLDRFVDNFLRPGNLQGGFDWYLSAAPSRRQVIEGTALPLPPITVPTRVLWGAR 257 Query: 255 DPLIAPEWSDRLGDHWSE-PSIRFVDTGHYVHAEAPGIVAEEASGFF 300 DP+I W DRL + +++ + R + GH+VH E+P + A E + FF Sbjct: 258 DPIIRVVWMDRLAETFTDLEAGRAEEAGHFVHVESPDLAAAEIARFF 304 >ref|YP_001768761.1| alpha/beta hydrolase fold protein [Methylobacterium sp. 4-46] gb|ACA16327.1| alpha/beta hydrolase fold [Methylobacterium sp. 4-46] Length = 320 Score = 292 bits (747), Expect = 5e-77, Method: Compositional matrix adjust. Identities = 156/291 (53%), Positives = 201/291 (69%), Gaps = 3/291 (1%) Query: 13 AAGITQQKIDIGGLRVNVARFGKGAPLLLLHGWPEFWLVWRPLMLRLGDSFELIAPDLRG 72 AA I + +D+ GLR +VAR G+G PL+LLHGWPEFW + PLM RL D F LIAPDLRG Sbjct: 8 AAEIRHEILDLPGLRQHVARAGEGPPLVLLHGWPEFWTSFEPLMARLSDRFSLIAPDLRG 67 Query: 73 CGDTGK-PTPGPDASATAERHALDMFAVMDALGHDRFGVIGGDLGAYVMQAMSHRQPQRL 131 G+TG+ P PD + A+ HA D+ A++DALG R G++G D+GAYVMQA + R P+RL Sbjct: 68 FGETGRDPAAPPDPTVDAQAHAADLLALLDALGLARVGLVGHDVGAYVMQAFARRHPERL 127 Query: 132 TGTLYLCTPYPGLGHRYGQPGHLIEVWYQYFQQLPWAAQLVGSSRESCRLYFRHFLDHWS 191 G + P G+G R+ HL E WYQYF Q P+AA LVG+SRESC Y HFL HWS Sbjct: 128 AGLFFFNCPTTGIGPRWAAAAHLKETWYQYFHQQPFAAALVGASRESCAAYIGHFLRHWS 187 Query: 192 GDDPAVFGDLLEAYVDNFMKPGNIQGGFDWYLSSAPNRRRWLEGKLPPQPRIDVPSRFLW 251 DP F ++E ++DNFM+PGN+QGGFDWYLS+AP R++ + G+ P P I VP+R LW Sbjct: 188 A-DPEAFAPMMERFIDNFMRPGNLQGGFDWYLSAAPGRQQVIAGEAPRLPPIPVPTRVLW 246 Query: 252 GRRDPLIAPEWSDRLGDHWSE-PSIRFVDTGHYVHAEAPGIVAEEASGFFG 301 G RDP+I EW D LG+ +S+ + R + GH+VH EAP + AE + FFG Sbjct: 247 GARDPIIRVEWMDALGETFSDLEAGRAEEAGHFVHVEAPDLAAEAIARFFG 297 >ref|YP_001924150.1| alpha/beta hydrolase fold protein [Methylobacterium populi BJ001] gb|ACB79615.1| alpha/beta hydrolase fold [Methylobacterium populi BJ001] Length = 294 Score = 289 bits (739), Expect = 4e-76, Method: Compositional matrix adjust. Identities = 140/275 (50%), Positives = 183/275 (66%), Gaps = 1/275 (0%) Query: 16 ITQQKIDIGGLRVNVARFGKGAPLLLLHGWPEFWLVWRPLMLRLGDSFELIAPDLRGCGD 75 I + + G++++VAR G G PL+LLHGWPEFWL W P+M RL D F LIAPDLRG G Sbjct: 4 IRHETVTANGVQLHVARAGAGRPLVLLHGWPEFWLTWEPVMARLADRFSLIAPDLRGFGA 63 Query: 76 TGKPTPGPDASATAERHALDMFAVMDALGHDRFGVIGGDLGAYVMQAMSHRQPQRLTGTL 135 + KP PGP A E HA D+ ++DALG +R GV+ D+GA V QA+ P+RLTG Sbjct: 64 SEKPDPGPSDRAGPEVHAADLVGLLDALGLERVGVVAHDVGASVGQALGRANPERLTGLF 123 Query: 136 YLCTPYPGLGHRYGQPGHLIEVWYQYFQQLPWAAQLVGSSRESCRLYFRHFLDHWSGDDP 195 + PYPG+G R +P L E+WYQ F P+AA +VG+SRESCR Y HFL HW+G +P Sbjct: 124 FFDCPYPGIGPRLARPEMLAEIWYQSFHLKPFAAGVVGASRESCRAYIGHFLSHWAGGNP 183 Query: 196 AVFGDLLEAYVDNFMKPGNIQGGFDWYLSSAPNRRRWLEGKLPPQPRIDVPSRFLWGRRD 255 + F D+L+A+ DNF+ PGN+QGGF+WYLS R + G+ P P I VP+ WG RD Sbjct: 184 SAFDDVLDAFTDNFLVPGNLQGGFNWYLSQQAERLAMVRGEAPALPPIGVPTCIRWGTRD 243 Query: 256 PLIAPEWSDRLGDHWSEPSIR-FVDTGHYVHAEAP 289 PL EW+DRLG+ +S+ + F +TGH+ H E P Sbjct: 244 PLFPYEWTDRLGETFSDLDLAPFEETGHFPHRERP 278 >ref|YP_001638925.1| alpha/beta hydrolase fold protein [Methylobacterium extorquens PA1] gb|ABY29854.1| alpha/beta hydrolase fold [Methylobacterium extorquens PA1] Length = 294 Score = 282 bits (721), Expect = 5e-74, Method: Compositional matrix adjust. Identities = 142/289 (49%), Positives = 184/289 (63%), Gaps = 1/289 (0%) Query: 16 ITQQKIDIGGLRVNVARFGKGAPLLLLHGWPEFWLVWRPLMLRLGDSFELIAPDLRGCGD 75 I + G++++VAR G G+PL+LLHGWPEFWL W P+M RL D F LIAPDLRG G Sbjct: 4 IRHDTVTGNGMQLHVARAGTGSPLVLLHGWPEFWLTWEPVMARLADRFSLIAPDLRGFGA 63 Query: 76 TGKPTPGPDASATAERHALDMFAVMDALGHDRFGVIGGDLGAYVMQAMSHRQPQRLTGTL 135 + KP GP A E HA D+ ++DALG R G++G D+GA V QA+ P RLTG Sbjct: 64 SEKPDHGPSDKAGPEVHAADIVGLLDALGLARAGIVGHDVGASVGQALGRSHPDRLTGLF 123 Query: 136 YLCTPYPGLGHRYGQPGHLIEVWYQYFQQLPWAAQLVGSSRESCRLYFRHFLDHWSGDDP 195 + PYPG+G R QP L E+WYQ F P+AA++VG+SRESCR Y HFL HW+G +P Sbjct: 124 FFDCPYPGIGPRLAQPEMLAEIWYQSFHLKPFAAEVVGASRESCRAYIGHFLRHWAGGNP 183 Query: 196 AVFGDLLEAYVDNFMKPGNIQGGFDWYLSSAPNRRRWLEGKLPPQPRIDVPSRFLWGRRD 255 + F +L+A+ DNF+ PGN+QGGF+WYLS R L G+ P P I VP+ WG +D Sbjct: 184 SAFDAVLDAFTDNFLVPGNLQGGFNWYLSRQAGRLAMLRGEAPALPPIRVPTCIRWGTQD 243 Query: 256 PLIAPEWSDRLGDHWSEPSIR-FVDTGHYVHAEAPGIVAEEASGFFGKL 303 PL W+DRLG+ +S + F GH+ H E P A + FFG+L Sbjct: 244 PLFPYAWTDRLGETFSNLDLAPFEGAGHFPHRERPEEAAAAIAAFFGRL 292 >ref|YP_002962483.1| alpha/beta hydrolase, epoxide hydrolase [methylobacterium extorquens AM1] gb|ACS39206.1| putative alpha/beta hydrolase, putative epoxide hydrolase [Methylobacterium extorquens AM1] Length = 294 Score = 281 bits (720), Expect = 8e-74, Method: Compositional matrix adjust. Identities = 141/289 (48%), Positives = 184/289 (63%), Gaps = 1/289 (0%) Query: 16 ITQQKIDIGGLRVNVARFGKGAPLLLLHGWPEFWLVWRPLMLRLGDSFELIAPDLRGCGD 75 I + G++++VAR G G+PL+LLHGWPEFWL W P+M RL D F LIAPDLRG G Sbjct: 4 IRHDTVTGNGVQLHVARAGTGSPLVLLHGWPEFWLTWEPVMARLADRFSLIAPDLRGFGA 63 Query: 76 TGKPTPGPDASATAERHALDMFAVMDALGHDRFGVIGGDLGAYVMQAMSHRQPQRLTGTL 135 + KP GP A E HA D+ ++DALG R G++G D+GA V QA+ P RLTG Sbjct: 64 SEKPDHGPSDKAGPEVHAADIVGLLDALGLARVGIVGHDVGASVGQALGRSHPDRLTGLF 123 Query: 136 YLCTPYPGLGHRYGQPGHLIEVWYQYFQQLPWAAQLVGSSRESCRLYFRHFLDHWSGDDP 195 + PYPG+G R QP L E+WYQ F P+AA++VG+SRESCR Y HFL HW+G +P Sbjct: 124 FFDCPYPGIGPRLAQPEMLAEIWYQSFHLKPFAAEVVGASRESCRAYIGHFLRHWAGGNP 183 Query: 196 AVFGDLLEAYVDNFMKPGNIQGGFDWYLSSAPNRRRWLEGKLPPQPRIDVPSRFLWGRRD 255 + F +L+A+ DNF+ PGN+QGGF+WYLS R + G+ P P I VP+ WG +D Sbjct: 184 SAFDAVLDAFTDNFLVPGNLQGGFNWYLSRQAGRLAMVRGEAPALPPIRVPTCIRWGTQD 243 Query: 256 PLIAPEWSDRLGDHWSEPSIR-FVDTGHYVHAEAPGIVAEEASGFFGKL 303 PL W+DRLG+ +S + F GH+ H E P A + FFG+L Sbjct: 244 PLFPYAWTDRLGETFSNLDLAPFEGAGHFPHRERPEEAAAAIAAFFGRL 292