BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0080.0073.p (314 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_001220565.1| Type IV secretory pathway AvhB4 protein [Bra... 586 e-165 ref|YP_002128528.1| VirB4 type IV secretion protein [Phenylobact... 455 e-126 ref|YP_001409520.1| type IV secretion/conjugal transfer ATPase [... 416 e-114 ref|YP_001369231.1| type IV secretion/conjugal transfer ATPase [... 415 e-114 ref|YP_004556245.1| type IV secretion/conjugal transfer ATPase, ... 414 e-113 >ref|YP_001220565.1| Type IV secretory pathway AvhB4 protein [Bradyrhizobium sp. BTAi1] gb|ABQ39893.1| Type IV secretory pathway AvhB4 protein [Bradyrhizobium sp. BTAi1] Length = 788 Score = 586 bits (1511), Expect = e-165, Method: Compositional matrix adjust. Identities = 283/310 (91%), Positives = 294/310 (94%) Query: 1 VRAAGGTYLTLRNGMPTGCAPLRALELTPTNLSFLGELVRKLVTPDDRPLTVVEEERIDS 60 VRA GGTYLTL NG+ TGCAPL+ALELTP NLSFLG L+RKLVTPD+RPLTV EEERIDS Sbjct: 475 VRAVGGTYLTLHNGIATGCAPLKALELTPANLSFLGALIRKLVTPDNRPLTVSEEERIDS 534 Query: 61 GLRALGQLRQAERSFGTLRTFLGQQDREGIGARLERWCRGAPFGWVLDGEEDAIGLEARF 120 GLRAL LRQ ERSFG LR FLGQQDREGIGARLERWCRGAPFGWVLDGE+DAIGL+ARF Sbjct: 535 GLRALVHLRQEERSFGALRAFLGQQDREGIGARLERWCRGAPFGWVLDGEQDAIGLDARF 594 Query: 121 IGFDMTDLLDHPTVRTPLMMYLFHRVERLIDGRRLIIDIDEFWKALGDEAFRDLANNKLK 180 +GFDMTDLLDHP+VRTPLMMYLFHRVERLIDGRRLIIDIDEFWKALGD AFRDLANNKLK Sbjct: 595 VGFDMTDLLDHPSVRTPLMMYLFHRVERLIDGRRLIIDIDEFWKALGDGAFRDLANNKLK 654 Query: 181 TIRKQNGVMVFGTQSPRDALASPIAHTIVEQCPTQIFLPNARGTLSDYVDGFHLTDTEFR 240 TIRKQNGVMVFGTQSPRDALASPIAHTIVEQCPTQIFLPNARGT SDYVDGFHLTDTEFR Sbjct: 655 TIRKQNGVMVFGTQSPRDALASPIAHTIVEQCPTQIFLPNARGTRSDYVDGFHLTDTEFR 714 Query: 241 LIKEELAPESRRFLVKQGHNSVVAELDLNGFDDALAVLSGRTETVELLDRIRNQVGDDPA 300 LIKEEL PESRRFL+KQGHNSVVAELDL GF+DALAVLSGRTETVELLDRIR +VGDDPA Sbjct: 715 LIKEELTPESRRFLIKQGHNSVVAELDLGGFNDALAVLSGRTETVELLDRIRAEVGDDPA 774 Query: 301 QWLPVFHAER 310 QWLPVFHAER Sbjct: 775 QWLPVFHAER 784 >ref|YP_002128528.1| VirB4 type IV secretion protein [Phenylobacterium zucineum HLK1] gb|ACG79953.1| VirB4 type IV secretion protein [Phenylobacterium zucineum HLK1] Length = 801 Score = 455 bits (1171), Expect = e-126, Method: Compositional matrix adjust. Identities = 222/312 (71%), Positives = 249/312 (79%) Query: 1 VRAAGGTYLTLRNGMPTGCAPLRALELTPTNLSFLGELVRKLVTPDDRPLTVVEEERIDS 60 VRA GG YL LRN PTG AP +ALE TP NL FLG+LVRKLVTP DRPLTV EE RID Sbjct: 488 VRACGGAYLPLRNAAPTGFAPFKALEYTPANLLFLGQLVRKLVTPADRPLTVQEERRIDD 547 Query: 61 GLRALGQLRQAERSFGTLRTFLGQQDREGIGARLERWCRGAPFGWVLDGEEDAIGLEARF 120 G++AL L QA RS LR LGQ+D EGIGARLERW R P GWVLD E D + LE RF Sbjct: 548 GIQALAPLPQANRSLQALRQLLGQRDPEGIGARLERWTRTGPLGWVLDNEADEMSLEGRF 607 Query: 121 IGFDMTDLLDHPTVRTPLMMYLFHRVERLIDGRRLIIDIDEFWKALGDEAFRDLANNKLK 180 +GFDMTD LD+P VRTP+MMYLFHR++ L DGRRL++DIDEFWKALGDEAFR LA + LK Sbjct: 608 LGFDMTDFLDNPEVRTPIMMYLFHRIDALADGRRLVVDIDEFWKALGDEAFRGLAQDGLK 667 Query: 181 TIRKQNGVMVFGTQSPRDALASPIAHTIVEQCPTQIFLPNARGTLSDYVDGFHLTDTEFR 240 T RK+N +MVFGTQSP D L SPIA TIVE C T+I+LPN G+ DYV+GF LT+ EFR Sbjct: 668 TYRKRNAMMVFGTQSPSDVLKSPIAPTIVEMCATKIYLPNPYGSAKDYVEGFGLTNEEFR 727 Query: 241 LIKEELAPESRRFLVKQGHNSVVAELDLNGFDDALAVLSGRTETVELLDRIRNQVGDDPA 300 LI+EELAPESR+FLVKQGHNSVV EL+LNG DD LA+LSGRTETVELLDRIR + GDDPA Sbjct: 728 LIREELAPESRQFLVKQGHNSVVVELNLNGLDDELAILSGRTETVELLDRIRARHGDDPA 787 Query: 301 QWLPVFHAERRK 312 W P+FH ERR Sbjct: 788 VWEPIFHIERRS 799 >ref|YP_001409520.1| type IV secretion/conjugal transfer ATPase [Xanthobacter autotrophicus Py2] gb|ABS70284.1| type IV secretion/conjugal transfer ATPase, VirB4 family [Xanthobacter autotrophicus Py2] Length = 790 Score = 416 bits (1069), Expect = e-114, Method: Compositional matrix adjust. Identities = 203/310 (65%), Positives = 234/310 (75%) Query: 1 VRAAGGTYLTLRNGMPTGCAPLRALELTPTNLSFLGELVRKLVTPDDRPLTVVEEERIDS 60 VRAAGGTYL L+NG PTGCAPL+ LELTP N FL + + KLV R L+V + I Sbjct: 478 VRAAGGTYLPLKNGTPTGCAPLKGLELTPENKVFLAQWIAKLVGSKSRELSVSDLRDIAG 537 Query: 61 GLRALGQLRQAERSFGTLRTFLGQQDREGIGARLERWCRGAPFGWVLDGEEDAIGLEARF 120 + L L R+ G LRTFL D EGI ARL RW RG P GWV D E D IG+ A+F Sbjct: 538 AVDGLADLPVQRRTIGALRTFLNNTDPEGIAARLRRWERGGPLGWVFDNETDDIGIGAKF 597 Query: 121 IGFDMTDLLDHPTVRTPLMMYLFHRVERLIDGRRLIIDIDEFWKALGDEAFRDLANNKLK 180 +G+DMTD LD+ +RTPLM YLFHRVE+LIDGRR+II IDEFWKAL DE FRDLA NKLK Sbjct: 598 LGYDMTDFLDNEEIRTPLMAYLFHRVEQLIDGRRIIIVIDEFWKALLDEGFRDLAQNKLK 657 Query: 181 TIRKQNGVMVFGTQSPRDALASPIAHTIVEQCPTQIFLPNARGTLSDYVDGFHLTDTEFR 240 TIRKQ G+M+F TQSPRDA+ASPIAHTI+EQCPTQIF+PN RG+ +DYVDGF LT+ E++ Sbjct: 658 TIRKQGGLMLFATQSPRDAIASPIAHTIIEQCPTQIFMPNPRGSHADYVDGFKLTEREYQ 717 Query: 241 LIKEELAPESRRFLVKQGHNSVVAELDLNGFDDALAVLSGRTETVELLDRIRNQVGDDPA 300 LI EL+PESRRF+VKQGHNSVVAEL+L GFDD LAVLSGRT VEL D IR +VG Sbjct: 718 LIARELSPESRRFIVKQGHNSVVAELNLAGFDDELAVLSGRTANVELADAIRAEVGSGQE 777 Query: 301 QWLPVFHAER 310 WLP+F R Sbjct: 778 DWLPLFQQRR 787 >ref|YP_001369231.1| type IV secretion/conjugal transfer ATPase [Ochrobactrum anthropi ATCC 49188] gb|ABS13402.1| type IV secretion/conjugal transfer ATPase, VirB4 family [Ochrobactrum anthropi ATCC 49188] Length = 796 Score = 415 bits (1066), Expect = e-114, Method: Compositional matrix adjust. Identities = 205/320 (64%), Positives = 239/320 (74%), Gaps = 10/320 (3%) Query: 1 VRAAGGTYLTLRNGMPTGCAPLRALELTPTNLSFLGELVRKLVTPDDRPLTVVEEERIDS 60 VRAAGGTYL L+NG+ TGCAPL+ALELTP N FL V KLV + R LTV E I + Sbjct: 474 VRAAGGTYLPLKNGIATGCAPLKALELTPENKVFLALWVGKLVRSNVRDLTVTELRDIAA 533 Query: 61 GLRALGQLRQAERSFGTLRTFLGQQDREGIGARLERWCRGAPFGWVLDGEEDAIGLE--- 117 + L L + R+ G LRTFL D EGI ARL RW R P GWV D ++D IGL Sbjct: 534 AIDGLSDLPRERRTIGALRTFLNNTDPEGIAARLRRWEREGPLGWVFDNDDDDIGLNEFA 593 Query: 118 -------ARFIGFDMTDLLDHPTVRTPLMMYLFHRVERLIDGRRLIIDIDEFWKALGDEA 170 +F+G+DMT+ LDH +RTPLM YLFHRVE+LIDGRR++I IDEFWKAL D+ Sbjct: 594 PMRAGRGGKFVGYDMTEFLDHEEIRTPLMAYLFHRVEQLIDGRRILIVIDEFWKALQDDG 653 Query: 171 FRDLANNKLKTIRKQNGVMVFGTQSPRDALASPIAHTIVEQCPTQIFLPNARGTLSDYVD 230 FRDLA NKLKTIRKQNG+M+F TQSPRDA+ SPIAHTI+EQCPTQIFLPN+RG +DYVD Sbjct: 654 FRDLAQNKLKTIRKQNGLMLFATQSPRDAIVSPIAHTIIEQCPTQIFLPNSRGNHADYVD 713 Query: 231 GFHLTDTEFRLIKEELAPESRRFLVKQGHNSVVAELDLNGFDDALAVLSGRTETVELLDR 290 GF LT+ E+ LI EL+ ESR+FL+KQGHNSVVAELDLNG DD LAVLSGRT VEL+D Sbjct: 714 GFKLTEREYELIARELSVESRQFLLKQGHNSVVAELDLNGLDDELAVLSGRTGNVELVDT 773 Query: 291 IRNQVGDDPAQWLPVFHAER 310 IR ++G+DPA WLPVF R Sbjct: 774 IRAEIGNDPADWLPVFQKRR 793 >ref|YP_004556245.1| type IV secretion/conjugal transfer ATPase, VirB4 family [Sphingobium chlorophenolicum L-1] gb|AEG51739.1| type IV secretion/conjugal transfer ATPase, VirB4 family [Sphingobium chlorophenolicum L-1] Length = 803 Score = 414 bits (1063), Expect = e-113, Method: Compositional matrix adjust. Identities = 201/314 (64%), Positives = 238/314 (75%) Query: 1 VRAAGGTYLTLRNGMPTGCAPLRALELTPTNLSFLGELVRKLVTPDDRPLTVVEEERIDS 60 VR+ GG YLTL+NG PTG APLRALE P NL FLG L+R+LVTP PL V +E ID Sbjct: 486 VRSVGGIYLTLKNGEPTGFAPLRALEYGPRNLVFLGRLIRQLVTPPGGPLGVTQERMIDE 545 Query: 61 GLRALGQLRQAERSFGTLRTFLGQQDREGIGARLERWCRGAPFGWVLDGEEDAIGLEARF 120 GL +LG+L RS LR LGQ+D EGIGARLE+W RG GWV D E DA+ L+A F Sbjct: 546 GLASLGRLAPENRSILALRQLLGQRDPEGIGARLEKWSRGGSLGWVFDNEIDALSLDAPF 605 Query: 121 IGFDMTDLLDHPTVRTPLMMYLFHRVERLIDGRRLIIDIDEFWKALGDEAFRDLANNKLK 180 +GFDMTD LD+P VRTP+MMY+FHR++ L+DGRRL+IDIDEFWKALGD+AFR A + LK Sbjct: 606 LGFDMTDFLDNPEVRTPIMMYMFHRIDGLLDGRRLVIDIDEFWKALGDDAFRAFAQDGLK 665 Query: 181 TIRKQNGVMVFGTQSPRDALASPIAHTIVEQCPTQIFLPNARGTLSDYVDGFHLTDTEFR 240 T RKQN +VFGTQSP DAL S I+H+I+EQ T+I LPN G +DY+DG LT EF+ Sbjct: 666 TYRKQNAFLVFGTQSPADALRSDISHSIMEQVATKILLPNPYGRETDYIDGLGLTQAEFK 725 Query: 241 LIKEELAPESRRFLVKQGHNSVVAELDLNGFDDALAVLSGRTETVELLDRIRNQVGDDPA 300 LI+ +L PESRRFL+KQGH+S+V ELDL G D LAVLSG TETV LLD IR VGDDPA Sbjct: 726 LIRHDLNPESRRFLIKQGHDSIVVELDLGGLTDELAVLSGTTETVTLLDAIRADVGDDPA 785 Query: 301 QWLPVFHAERRKAQ 314 +WLP+FH +RR A Sbjct: 786 KWLPLFHQQRRSAS 799