BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0080.0072.p (451 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_001220565.1| Type IV secretory pathway AvhB4 protein [Bra... 714 0.0 ref|YP_001985528.1| transport secretion system IV protein, VirB4... 506 e-141 gb|EGE59354.1| transport secretion system IV protein, VirB4 [Rhi... 503 e-140 ref|YP_665963.1| type IV secretion/conjugal transfer ATPase [Mes... 503 e-140 ref|NP_659891.1| transport secretion system IV, VirB4 protein [R... 500 e-139 >ref|YP_001220565.1| Type IV secretory pathway AvhB4 protein [Bradyrhizobium sp. BTAi1] gb|ABQ39893.1| Type IV secretory pathway AvhB4 protein [Bradyrhizobium sp. BTAi1] Length = 788 Score = 714 bits (1843), Expect = 0.0, Method: Compositional matrix adjust. Identities = 350/410 (85%), Positives = 381/410 (92%) Query: 1 MRNATLKTREIEPNVYLPFGRHVTDTVISLTTRALLTVIRIEGTSFETAETSDLNNLHGK 60 MRNA LKTREI P VYLPFGRHVTDTVISLTTRA+LTVIR++GTSFETAET+DLN+LH K Sbjct: 1 MRNAALKTREIGPEVYLPFGRHVTDTVISLTTRAMLTVIRLDGTSFETAETADLNDLHAK 60 Query: 61 LNLTLRNVADPRLALWTHLVRRRTSVYPDGTFRSAFAAGLDAQYHERLQKEALFRNDLYL 120 LNLTLRNVADPRLALWTHLVRRRTS YPDG FRS FAA LDAQY +RL+K ALFRNDLY+ Sbjct: 61 LNLTLRNVADPRLALWTHLVRRRTSDYPDGAFRSPFAASLDAQYRDRLRKAALFRNDLYV 120 Query: 121 TLVCHPGRAATDKAAAFFRRLRQSAGHSVEVDGEALKRLNDATRDVIAALDRYGARAVGV 180 TLVCHPGRA D AAAFFRRL +SA S+EVD +ALKRLNDATRD++AAL+RYGA A+G+ Sbjct: 121 TLVCHPGRAPADAAAAFFRRLGRSAAASLEVDSDALKRLNDATRDMVAALERYGAHALGL 180 Query: 181 AERDGIIFSEPMELLHALASGEWMPMPLPQGPIGPALYSNRVIFGRETLEIRAAGGSRFA 240 AERDGI+FSEPMEL HA+ASGEW MPLPQGPIGPALYSNRVIFGRE +E R+AGGSRFA Sbjct: 181 AERDGIVFSEPMELFHAVASGEWTSMPLPQGPIGPALYSNRVIFGREAIETRSAGGSRFA 240 Query: 241 GIFGLKEYPASTRPGLLNALLSAPFELVVAQSFAFLAKADAKTVLTRKQNQLVSAQDPAA 300 G+FGLKEYPAST+PGLLNALLSAPFEL++ QSFAFL+K+DAKTVLTRKQNQLVSAQDPAA Sbjct: 241 GMFGLKEYPASTKPGLLNALLSAPFELILTQSFAFLSKSDAKTVLTRKQNQLVSAQDPAA 300 Query: 301 SQIDELSDALDDLESNRFALGDHHLSLLVYADTPSQLQGQMSVARRALADAGAVVAREDL 360 SQIDEL+DALDDLESNRFALGDHHLSLL+YA+TPSQLQ MSVARR LADAGAVVAREDL Sbjct: 301 SQIDELADALDDLESNRFALGDHHLSLLIYAETPSQLQDHMSVARRVLADAGAVVAREDL 360 Query: 361 GLEAAYWAQLPGLFKYRARTGAINSRNFAALSSFHTYPTGQPAGNHWGPA 410 GLEAAYWAQLPGLFKYRARTGAI+SRNFAA S FHTYPTGQP+GNHWGPA Sbjct: 361 GLEAAYWAQLPGLFKYRARTGAISSRNFAAFSPFHTYPTGQPSGNHWGPA 410 >ref|YP_001985528.1| transport secretion system IV protein, VirB4 [Rhizobium etli CIAT 652] gb|ACE94978.1| transport secretion system IV protein, VirB4 [Rhizobium etli CIAT 652] Length = 788 Score = 506 bits (1302), Expect = e-141, Method: Compositional matrix adjust. Identities = 242/407 (59%), Positives = 312/407 (76%) Query: 4 ATLKTREIEPNVYLPFGRHVTDTVISLTTRALLTVIRIEGTSFETAETSDLNNLHGKLNL 63 ATL++RE+ P ++P+ RHV +T I+L +RAL+T+I ++G SFET++ DLN LH LN Sbjct: 5 ATLRSRELGPETFIPYVRHVDETTIALESRALMTMIALDGVSFETSDIRDLNGLHRNLNT 64 Query: 64 TLRNVADPRLALWTHLVRRRTSVYPDGTFRSAFAAGLDAQYHERLQKEALFRNDLYLTLV 123 RN+AD RLALWTH+VRRR + YPDG F +AF+ GL+A+Y R+ +E LFRNDLYLTLV Sbjct: 65 LYRNIADERLALWTHVVRRRDNAYPDGRFANAFSDGLNAKYRHRMVREDLFRNDLYLTLV 124 Query: 124 CHPGRAATDKAAAFFRRLRQSAGHSVEVDGEALKRLNDATRDVIAALDRYGARAVGVAER 183 HPGR ++AA RLR++ +E+D ALK L D D++A L R+ R + + E+ Sbjct: 125 WHPGRDPAERAANLLSRLRKARRSGIELDEHALKHLRDRVADLVAGLQRFEPRLLSLHEQ 184 Query: 184 DGIIFSEPMELLHALASGEWMPMPLPQGPIGPALYSNRVIFGRETLEIRAAGGSRFAGIF 243 DGI+FSEP E+LH L G P+ L +G I A+YS+RVIFGRET+EIR SR+AG+F Sbjct: 185 DGILFSEPSEVLHQLVGGRQEPIALTEGRISSAIYSDRVIFGRETVEIRHEADSRYAGMF 244 Query: 244 GLKEYPASTRPGLLNALLSAPFELVVAQSFAFLAKADAKTVLTRKQNQLVSAQDPAASQI 303 GLKEYPA+TR G+L+A+L+APFEL++ QSFAF +KADA+T++ RKQNQ+VSA D AASQI Sbjct: 245 GLKEYPATTRSGMLDAILTAPFELILTQSFAFASKADARTIMGRKQNQMVSAGDKAASQI 304 Query: 304 DELSDALDDLESNRFALGDHHLSLLVYADTPSQLQGQMSVARRALADAGAVVAREDLGLE 363 +EL DA+DDLESNRFALG+HHL+L V+A + QL M+ AR L GAVVAREDLGLE Sbjct: 305 EELGDAMDDLESNRFALGEHHLALAVFAPSVKQLTDHMAKARAYLTSGGAVVAREDLGLE 364 Query: 364 AAYWAQLPGLFKYRARTGAINSRNFAALSSFHTYPTGQPAGNHWGPA 410 AA+WAQLPG F+YRAR+GAI SRNFAAL+ +H+YPTGQ GN WGPA Sbjct: 365 AAWWAQLPGNFRYRARSGAITSRNFAALAPYHSYPTGQKDGNEWGPA 411 >gb|EGE59354.1| transport secretion system IV protein, VirB4 [Rhizobium etli CNPAF512] Length = 788 Score = 503 bits (1295), Expect = e-140, Method: Compositional matrix adjust. Identities = 238/407 (58%), Positives = 312/407 (76%) Query: 4 ATLKTREIEPNVYLPFGRHVTDTVISLTTRALLTVIRIEGTSFETAETSDLNNLHGKLNL 63 ATL++RE+ P ++P+ RHV +T I+L +RAL+T+I ++G SFET++ DLN+LH LN Sbjct: 5 ATLRSRELGPETFIPYVRHVDETTIALESRALMTMIALDGVSFETSDVRDLNDLHRSLNT 64 Query: 64 TLRNVADPRLALWTHLVRRRTSVYPDGTFRSAFAAGLDAQYHERLQKEALFRNDLYLTLV 123 RN+AD RLALWTHLVRRR + YP+G F + F+ GL+A+Y ER+ +E LFRNDLYLTLV Sbjct: 65 LYRNIADERLALWTHLVRRRDNAYPEGRFANPFSDGLNARYRERMVREDLFRNDLYLTLV 124 Query: 124 CHPGRAATDKAAAFFRRLRQSAGHSVEVDGEALKRLNDATRDVIAALDRYGARAVGVAER 183 HPGR +KAA RLR++ E+DG+ALK L D DV+A L R+ R + + E+ Sbjct: 125 WHPGRDPAEKAAKLLSRLRKARRSDTELDGQALKHLRDRVADVVAGLKRFEPRLLTLHEQ 184 Query: 184 DGIIFSEPMELLHALASGEWMPMPLPQGPIGPALYSNRVIFGRETLEIRAAGGSRFAGIF 243 DGI+FSEP E+LH + G P+PL +G I A+YS+RVIFGRET+EIR SR+AG+F Sbjct: 185 DGILFSEPSEVLHQIVGGRREPIPLTEGRISSAIYSDRVIFGRETVEIRHEADSRYAGMF 244 Query: 244 GLKEYPASTRPGLLNALLSAPFELVVAQSFAFLAKADAKTVLTRKQNQLVSAQDPAASQI 303 G KEYPA+TR G+L+A+L+APFEL++ QSFAF +KADA+T++ RKQNQ+VSA D AASQI Sbjct: 245 GFKEYPATTRSGMLDAILTAPFELILTQSFAFTSKADARTIMGRKQNQMVSAGDKAASQI 304 Query: 304 DELSDALDDLESNRFALGDHHLSLLVYADTPSQLQGQMSVARRALADAGAVVAREDLGLE 363 +EL +A+DDLESNRF LG+HHL+L V+A + +L M+ AR L GAVVAREDLGLE Sbjct: 305 EELGEAMDDLESNRFVLGEHHLALAVFAPSVKELTDHMAKARAYLTSGGAVVAREDLGLE 364 Query: 364 AAYWAQLPGLFKYRARTGAINSRNFAALSSFHTYPTGQPAGNHWGPA 410 AA+WAQ+PG F+YRAR+GAI SRNFAAL+ +H+YP G+ GN WGPA Sbjct: 365 AAWWAQVPGNFRYRARSGAITSRNFAALAPYHSYPAGKKDGNEWGPA 411 >ref|YP_665963.1| type IV secretion/conjugal transfer ATPase [Mesorhizobium sp. BNC1] gb|ABG61316.1| type IV secretion/conjugal transfer ATPase, VirB4 family [Chelativorans sp. BNC1] Length = 793 Score = 503 bits (1294), Expect = e-140, Method: Compositional matrix adjust. Identities = 237/407 (58%), Positives = 308/407 (75%) Query: 4 ATLKTREIEPNVYLPFGRHVTDTVISLTTRALLTVIRIEGTSFETAETSDLNNLHGKLNL 63 AT+++RE+EP ++P+ RH+ + VI+L +RAL+ VI +EG SFETA+T DLN LH LN Sbjct: 5 ATVRSRELEPETFIPYVRHIDERVIALDSRALMVVIALEGVSFETADTLDLNALHRDLNT 64 Query: 64 TLRNVADPRLALWTHLVRRRTSVYPDGTFRSAFAAGLDAQYHERLQKEALFRNDLYLTLV 123 RN+AD RLALWTH+VRRR + YPDG F +AF+ L+ +Y R+ E LFRNDLYL+LV Sbjct: 65 LYRNIADERLALWTHVVRRRDNEYPDGEFANAFSRTLNDKYRNRMIGEDLFRNDLYLSLV 124 Query: 124 CHPGRAATDKAAAFFRRLRQSAGHSVEVDGEALKRLNDATRDVIAALDRYGARAVGVAER 183 HPG+ A ++ AAF RLR++ VE+D +ALKRL+D DV A L R+G R + + ER Sbjct: 125 WHPGKDAAERVAAFLSRLRKARRRGVELDVDALKRLDDTIVDVTAGLKRFGPRVLTLQER 184 Query: 184 DGIIFSEPMELLHALASGEWMPMPLPQGPIGPALYSNRVIFGRETLEIRAAGGSRFAGIF 243 DG++FSEP E+LH L G +PL +G + A+YS+RVI GRET+EIR G SR+AG+F Sbjct: 185 DGLVFSEPSEVLHQLVGGRREAVPLTEGRVSSAIYSDRVIVGRETVEIRHEGSSRYAGMF 244 Query: 244 GLKEYPASTRPGLLNALLSAPFELVVAQSFAFLAKADAKTVLTRKQNQLVSAQDPAASQI 303 G KEYPA TRPG+ + LL+ P+EL++ QSF+FL+K+DAKTV+ RKQNQ+VSA D A SQ+ Sbjct: 245 GFKEYPARTRPGMFDGLLTVPYELILTQSFSFLSKSDAKTVMGRKQNQMVSAGDKAGSQL 304 Query: 304 DELSDALDDLESNRFALGDHHLSLLVYADTPSQLQGQMSVARRALADAGAVVAREDLGLE 363 +EL DALDDLESNRF LG+HHL+L V+A T +L ++ +R L + GAVVAREDLGLE Sbjct: 305 EELDDALDDLESNRFVLGNHHLTLTVFAGTVRELTDNLAKSRTHLTNGGAVVAREDLGLE 364 Query: 364 AAYWAQLPGLFKYRARTGAINSRNFAALSSFHTYPTGQPAGNHWGPA 410 AA+WAQLPG F+YR R+GAI S+NFAA S FH+YP GQ GN WGPA Sbjct: 365 AAWWAQLPGNFRYRVRSGAITSKNFAAFSPFHSYPVGQRDGNEWGPA 411 >ref|NP_659891.1| transport secretion system IV, VirB4 protein [Rhizobium etli CFN 42] gb|AAM54904.1| transport secretion system IV, VirB4 protein [Rhizobium etli CFN 42] Length = 795 Score = 500 bits (1288), Expect = e-139, Method: Compositional matrix adjust. Identities = 238/407 (58%), Positives = 308/407 (75%) Query: 4 ATLKTREIEPNVYLPFGRHVTDTVISLTTRALLTVIRIEGTSFETAETSDLNNLHGKLNL 63 ATL++RE+ P ++P+ RHV +T I+L +RAL+T+I ++G SFET++ DLN LH LN Sbjct: 12 ATLRSRELGPETFIPYVRHVDETTIALESRALMTMIALDGVSFETSDVRDLNGLHRSLNT 71 Query: 64 TLRNVADPRLALWTHLVRRRTSVYPDGTFRSAFAAGLDAQYHERLQKEALFRNDLYLTLV 123 RN+AD RLALWTH+VRRR + YP+G F + F+ GL+A+Y ER+ E LFRNDLYLTLV Sbjct: 72 LYRNIADERLALWTHVVRRRDNAYPEGRFANPFSDGLNARYRERMVNENLFRNDLYLTLV 131 Query: 124 CHPGRAATDKAAAFFRRLRQSAGHSVEVDGEALKRLNDATRDVIAALDRYGARAVGVAER 183 HPGR +KAA RL ++ E+DG+ALK L D DV A L R+G R + + E Sbjct: 132 WHPGRDPAEKAAKLLSRLSKARRSDTELDGQALKHLRDHVADVAAGLQRFGPRQLTLHEH 191 Query: 184 DGIIFSEPMELLHALASGEWMPMPLPQGPIGPALYSNRVIFGRETLEIRAAGGSRFAGIF 243 DGI+FSEP E+LH + G P+PL +G I A+YS+RVIFGRET+EIR SR+AG+F Sbjct: 192 DGILFSEPSEVLHQIVGGRREPIPLTEGRISSAIYSDRVIFGRETIEIRHEADSRYAGMF 251 Query: 244 GLKEYPASTRPGLLNALLSAPFELVVAQSFAFLAKADAKTVLTRKQNQLVSAQDPAASQI 303 G KEYPA+TR G+L+A+L+APFEL++ QSFAF +KADA+T++ RKQNQ+VSA D AASQI Sbjct: 252 GFKEYPATTRSGMLDAILTAPFELILTQSFAFTSKADARTIMGRKQNQMVSAGDKAASQI 311 Query: 304 DELSDALDDLESNRFALGDHHLSLLVYADTPSQLQGQMSVARRALADAGAVVAREDLGLE 363 +EL +A+DDLESNRF LG+HHL+L V+A + +L M+ AR L GAVVAREDLGLE Sbjct: 312 EELGEAMDDLESNRFVLGEHHLTLAVFAPSVKELTDHMAKARAYLTSGGAVVAREDLGLE 371 Query: 364 AAYWAQLPGLFKYRARTGAINSRNFAALSSFHTYPTGQPAGNHWGPA 410 AA+WAQLPG F+YRAR+GAI SRNFAAL+ +H+YP G+ GN WGPA Sbjct: 372 AAWWAQLPGNFRYRARSGAITSRNFAALAPYHSYPAGKKDGNEWGPA 418