BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0077.0003.p (485 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_771179.1| hypothetical protein blr4539 [Bradyrhizobium ja... 744 0.0 ref|YP_001770766.1| hypothetical protein M446_3966 [Methylobacte... 488 e-136 ref|YP_002499846.1| nitroreductase [Methylobacterium nodulans OR... 472 e-131 ref|ZP_08073472.1| SagB-type dehydrogenase domain [Methylocystis... 428 e-117 ref|YP_003900368.1| SagB-type dehydrogenase domain-containing pr... 242 8e-62 >ref|NP_771179.1| hypothetical protein blr4539 [Bradyrhizobium japonicum USDA 110] dbj|BAC49804.1| blr4539 [Bradyrhizobium japonicum USDA 110] Length = 485 Score = 744 bits (1920), Expect = 0.0, Method: Compositional matrix adjust. Identities = 368/480 (76%), Positives = 405/480 (84%) Query: 1 LRNFRKTKLRKIAPVIVARLNGRCSLHMQADGSIAAGLGGYSINLGQFSAAAVDRAGHLT 60 +R RK RKIAP I ARL+ R SL MQ DG++ A LG YS+NLGQFS AA+DRA HL+ Sbjct: 1 MRAPRKKPTRKIAPDIAARLSHRFSLQMQPDGNLVAFLGDYSVNLGQFSKAAIDRARHLS 60 Query: 61 SGLPLASFAGKGVLARQVHALVRRLAGHGFLEYRLYSPRDEQDLVVIEPQVAEYWPQRAK 120 +GLPLASFAGK +AR V ALV RLA G LEYRL S DE+DLVVIEPQV EYWP+RAK Sbjct: 61 TGLPLASFAGKSAVARDVDALVHRLARQGLLEYRLASSCDERDLVVIEPQVPEYWPRRAK 120 Query: 121 LGNGDTIVLSRFAYLRRRGSEMVLESPRAGALFRIFDPAIAATLAALSRPQKISNLRRHA 180 LGN DTIVLSRFAYLRRRG+EMVLESPRAGALFRI DPA+AATLAALS+PQKI L R A Sbjct: 121 LGNRDTIVLSRFAYLRRRGNEMVLESPRAGALFRIGDPAVAATLAALSQPQKIGKLNRKA 180 Query: 181 ASANLDLLGLLIDSKMLIKLNAKDGDGLRVNEGDGNLVLWDFHDLVFHTRSTEGRHTNPV 240 A+ NL LL LL++S++L+KL+AKDG GLRVNEGDGNLVLWDFHDLVFHTRSTEGR NPV Sbjct: 181 ATFNLCLLELLVESRILLKLDAKDGQGLRVNEGDGNLVLWDFHDLVFHTRSTEGRQANPV 240 Query: 241 GGAFTHAGVVPPLPAVRPPWPGKKIDLGKFSTSGSVSHFAKLLRERHSIRDFDDQHPVTL 300 G AFT+A +VPPLPAVRPPWPG KIDL KFS+ +S F KLLRERHS RDFDD++PVTL Sbjct: 241 GAAFTYASIVPPLPAVRPPWPGSKIDLRKFSSPEPISLFPKLLRERHSTRDFDDKNPVTL 300 Query: 301 AELAQFLDTTARVLSEWKIGADFDGVPEATYSARPYPSAGGAYELELYLAVLNCGELARG 360 ELAQFLDTTARV+SEWK G FDG P+ TYS RPYPSAG AYELELYL V NC LARG Sbjct: 301 GELAQFLDTTARVVSEWKSGPYFDGGPDVTYSTRPYPSAGSAYELELYLTVSNCEGLARG 360 Query: 361 LYHYDAGSHALVAIDTSAQQLQALSTAAEFAMDASGPPQVLITIAARFGRISWKYSSIAY 420 LYHYDAGSHALVAI S QQLQA + AA+FAMDA G PQ+LITIAARFGR+SWKYSSIAY Sbjct: 361 LYHYDAGSHALVAISASTQQLQAHAVAAQFAMDAPGQPQILITIAARFGRVSWKYSSIAY 420 Query: 421 SLILKDVGNLMQTFYLTATDMGLGGCAIGTCNIDLFAKMTGLEFHVESPVGQFALGRGRK 480 SLILKDVG+L+QTFYL ATDM LGGCAIG+ NIDLFA MT LEFH+E PVGQFALGRGRK Sbjct: 421 SLILKDVGSLIQTFYLAATDMDLGGCAIGSTNIDLFATMTELEFHIEGPVGQFALGRGRK 480 >ref|YP_001770766.1| hypothetical protein M446_3966 [Methylobacterium sp. 4-46] gb|ACA18332.1| conserved hypothetical protein [Methylobacterium sp. 4-46] Length = 491 Score = 488 bits (1256), Expect = e-136, Method: Compositional matrix adjust. Identities = 259/465 (55%), Positives = 320/465 (68%), Gaps = 7/465 (1%) Query: 19 RLNGRCSLHMQADGSIAAGLGGYSINLGQFSAAAVDRAGHLTSGLPLASF-AGKGVLARQ 77 RL +L A G I + I+LG+F V+R L++GLP+ + A + R Sbjct: 20 RLRRHIALDAGAGGEILVRVEHQEISLGRFDECVVERMRALSAGLPVGALVASPDRVDRD 79 Query: 78 VHALVRRLAGHGFLEYRLYSPRDEQDLVVIEPQVAEYWPQRAKLGNGDTIVLSRFAYLRR 137 V LV+RLA G +EY L D + VVIEPQV +YWP+ L +GD++VLSRFAYLRR Sbjct: 80 VLRLVQRLARSGLVEYSLAGA-DHRGEVVIEPQVPDYWPRLPDLTDGDSVVLSRFAYLRR 138 Query: 138 RGSEMVLESPRAGALFRIFDPAIAATLAALSRPQKISNLRRHAASANLDLLGLLIDSKML 197 R S+MVLESPR+ ALFRI DPA+AA LA LS PQ ++ LRR +LL LL+DS++L Sbjct: 139 RASDMVLESPRSAALFRIGDPAVAALLAGLSEPQPVARLRRQDGLPGAELLALLLDSQIL 198 Query: 198 IKLNAKDGDGLRVNEGDGNLVLWDFHDLVFHTRSTEGRHTNPVGGAFTHAGVVPPLPAVR 257 +++ A GLR EGD +LVLWDFHDL+FH RSTEGRH NP+GG + + G+ PLPAVR Sbjct: 199 LRIEADGDAGLRATEGDEHLVLWDFHDLLFHARSTEGRHANPLGGRYPYVGLFNPLPAVR 258 Query: 258 PPWPGKKIDLGKFST--SGSVSHFAKLLRERHSIRDFDDQHPVTLAELAQFLDTTARVLS 315 P WPG+ IDL K S +V + LLR+R S R +DDQ P+T AELAQ LD+ ARV + Sbjct: 259 PRWPGRAIDLRKDSDVPGTTVRPVSALLRKRRSTRSYDDQAPLTRAELAQLLDSVARVQA 318 Query: 316 EWK--IGADFDGVPEATYSARPYPSAGGAYELELYLAVLNCGELARGLYHYDAGSHALVA 373 W+ + A DG P +Y+ RPYPSAG +YELELYLA+ +C LA G YHYDAG HAL Sbjct: 319 TWRSPLDAKHDG-PILSYTTRPYPSAGASYELELYLAIRHCDGLAPGYYHYDAGRHALTL 377 Query: 374 IDTSAQQLQALSTAAEFAMDASGPPQVLITIAARFGRISWKYSSIAYSLILKDVGNLMQT 433 + Q L AL TA +AM PQVLITIAARFGR+SWKYS++AY LILK+VG L QT Sbjct: 378 LPVRPQDLNALLTAGAYAMGEPCLPQVLITIAARFGRVSWKYSAVAYELILKNVGVLTQT 437 Query: 434 FYLTATDMGLGGCAIGTCNIDLFAKMTGLEFHVESPVGQFALGRG 478 YL AT+MGLGGCAIGT +I+LFA+MTGL HVE VGQFALGRG Sbjct: 438 LYLVATEMGLGGCAIGTADIELFARMTGLPHHVEGAVGQFALGRG 482 >ref|YP_002499846.1| nitroreductase [Methylobacterium nodulans ORS 2060] gb|ACL59543.1| nitroreductase [Methylobacterium nodulans ORS 2060] Length = 492 Score = 472 bits (1214), Expect = e-131, Method: Compositional matrix adjust. Identities = 263/479 (54%), Positives = 330/479 (68%), Gaps = 6/479 (1%) Query: 5 RKTKLRKIAPVIVA-RLNGRCSLHMQADGSIAAGLGGYSINLGQFSAAAVDRAGHLTSGL 63 R ++ +P ++ RL+ +L A G I + +++LG F A V+ L++GL Sbjct: 5 RTAHIQAASPATLSVRLSRHIALEAAAGGEILVRVEHQAVSLGTFDACVVEHMRALSAGL 64 Query: 64 PLASFAG-KGVLARQVHALVRRLAGHGFLEYRLYSPRDEQDLVVIEPQVAEYWPQRAKLG 122 P+ A + + R+V LV+RLA G +EY L D VVIEPQV +YWPQ L Sbjct: 65 PVDLLAASRDSVDREVLRLVQRLARSGLVEYSLVRA-DRGGEVVIEPQVPDYWPQLPDLT 123 Query: 123 NGDTIVLSRFAYLRRRGSEMVLESPRAGALFRIFDPAIAATLAALSRPQKISNLRRHAAS 182 +GDT+VLSRFAY+RRR SEMVLESPR+GALFR+ DPA AA LA+LS PQ ++ LRR Sbjct: 124 DGDTLVLSRFAYMRRRASEMVLESPRSGALFRLGDPAAAAMLASLSEPQPVARLRRQDGF 183 Query: 183 ANLDLLGLLIDSKMLIKLNAKDGDGLRVNEGDGNLVLWDFHDLVFHTRSTEGRHTNPVGG 242 +LL LL+DS++L+++ A GLR EGD +LVLWDFHD +FHTRSTEGRH NP+GG Sbjct: 184 PGTELLALLLDSQILLRIEADGDAGLRATEGDEHLVLWDFHDFLFHTRSTEGRHANPLGG 243 Query: 243 AFTHAGVVPPLPAVRPPWPGKKIDLGKFSTS--GSVSHFAKLLRERHSIRDFDDQHPVTL 300 + + G+ P PA+RP WPG+ IDL + S + +V LLRER S R FDD+ P+T Sbjct: 244 RYPYVGLSNPPPAIRPRWPGRAIDLRRDSDTPGATVRPVTALLRERRSTRSFDDRAPLTR 303 Query: 301 AELAQFLDTTARVLSEWKIGADFD-GVPEATYSARPYPSAGGAYELELYLAVLNCGELAR 359 AELA+ LD+ ARV + W D + G P +Y+ARPYPSAG +YELELYLAV +C LA Sbjct: 304 AELARLLDSAARVQATWCSPLDGEHGGPMMSYTARPYPSAGASYELELYLAVRHCDGLAP 363 Query: 360 GLYHYDAGSHALVAIDTSAQQLQALSTAAEFAMDASGPPQVLITIAARFGRISWKYSSIA 419 G YHYDAG HAL + Q L AL AA FAM A GPPQVLITIAARFGR+SWKYS++A Sbjct: 364 GYYHYDAGRHALTLLHVRPQDLDALLAAAAFAMGAPGPPQVLITIAARFGRVSWKYSAVA 423 Query: 420 YSLILKDVGNLMQTFYLTATDMGLGGCAIGTCNIDLFAKMTGLEFHVESPVGQFALGRG 478 Y LILK+VG L QT YL AT+MGLGGCAIG+ NI+LFA+MTGL H+E VGQFALGRG Sbjct: 424 YELILKNVGVLTQTLYLVATEMGLGGCAIGSANIELFARMTGLPHHIEGAVGQFALGRG 482 >ref|ZP_08073472.1| SagB-type dehydrogenase domain [Methylocystis sp. ATCC 49242] gb|EFX98880.1| SagB-type dehydrogenase domain [Methylocystis sp. ATCC 49242] Length = 478 Score = 428 bits (1100), Expect = e-117, Method: Compositional matrix adjust. Identities = 228/461 (49%), Positives = 305/461 (66%), Gaps = 3/461 (0%) Query: 16 IVARLNGRCSLHMQADGSIAAGLGGYSINLGQFSAAAVDRAGHLTSGLPLASFAGKGVLA 75 + ARLN ++ G ++A G + LG+FS ++ A L SG+ L + + Sbjct: 12 LFARLNPGVTVEDDGAGGLSAFFDGRGLRLGKFSPGLIECAADLASGVALEDDRDREI-E 70 Query: 76 RQVHALVRRLAGHGFLEYRLYSPRDEQDLVVIEPQVAEYWPQRAKLGNGDTIVLSRFAYL 135 +++ LVRRLA +G +EYRL +D DL+V+EPQ+ +Y P+ + L + +VLSRFAY+ Sbjct: 71 QEIGELVRRLALYGLVEYRLACAQDGPDLMVVEPQIRDYAPRISGLDDDRALVLSRFAYV 130 Query: 136 RRRGSEMVLESPRAGALFRIFDPAIAATLAALSRPQKISNLRRHAASANLDLLGLLIDSK 195 RRRG++M LESPRAGALFR+ P+ A + LS P+ + LR ++ L LL+D+ Sbjct: 131 RRRGADMALESPRAGALFRLCAPSALAMIGRLSEPRTVRELRDAPDFPGVEFLALLLDAD 190 Query: 196 MLIKLNAKDGDGLRVNEGDGNLVLWDFHDLVFHTRSTEGRHTNPVGGAFTHAGVVPPLPA 255 +L GLR EGD +L+LWDFHDL+FHTRST GRH NP GG + A +VPP A Sbjct: 191 ILFTPPPGADRGLRAAEGDNDLILWDFHDLLFHTRSTNGRHANPAGGLYARAHLVPPPAA 250 Query: 256 VRPPWPGKKIDLGKFSTSGSVSHFAKLLRERHSIRDFDDQHPVTLAELAQFLDTTARVLS 315 VRP WPG I+L T+ S FA++LR RHS R++D HP+T A++A+ LD AR++S Sbjct: 251 VRPGWPGTAIELTGLDTA-PASPFAQMLRRRHSTRNYDAHHPITAAQVARLLDGAARIIS 309 Query: 316 EWKIGADFDGVPEATYSARPYPSAGGAYELELYLAVLNCGELARGLYHYDAGSHALVAID 375 ++ D +G +ARPYPS G +YELELYLAV C LARG YHYDA HALV I+ Sbjct: 310 RERLRDD-EGGEGFEIAARPYPSGGASYELELYLAVDKCDGLARGFYHYDADRHALVPIE 368 Query: 376 TSAQQLQALSTAAEFAMDASGPPQVLITIAARFGRISWKYSSIAYSLILKDVGNLMQTFY 435 T+ + L A+ + AM AS PPQ+LIT+AARFGR+SWKYS AY L+LK VG +MQT Y Sbjct: 369 TNERLLAAMLEDGQLAMGASAPPQILITMAARFGRVSWKYSGFAYGLVLKHVGVVMQTLY 428 Query: 436 LTATDMGLGGCAIGTCNIDLFAKMTGLEFHVESPVGQFALG 476 L AT+M +G CAIG +IDLF++MTGLEFHVE VGQ A+G Sbjct: 429 LMATEMEIGACAIGVEDIDLFSRMTGLEFHVEGAVGQMAIG 469 >ref|YP_003900368.1| SagB-type dehydrogenase domain-containing protein [Cyanothece sp. PCC 7822] gb|ADN18302.1| SagB-type dehydrogenase domain protein [Cyanothece sp. PCC 7822] Length = 458 Score = 242 bits (618), Expect = 8e-62, Method: Compositional matrix adjust. Identities = 140/359 (38%), Positives = 199/359 (55%), Gaps = 25/359 (6%) Query: 125 DTIVLSRFAYLRRRGSEMVLESPRAGALFRIFDPAIAATLAALSRPQKISNLRRHAASAN 184 T LSRFAYL + +M+LESPR+ A + D A + LS+PQ ++L Sbjct: 115 QTFTLSRFAYLHQIEGQMLLESPRSKAKIILLDWRATALIGKLSQPQSCASLAEEIPDLT 174 Query: 185 LDL----LGLLIDSKMLIKLNAKDGDGLRVNEGDGNLVLWDFHDLVFHTRSTEGRHTNPV 240 +L + LL+ +KML L A++ +L+ W+FHDL+FH+RS +GRH+NPV Sbjct: 175 EELTQQFISLLLATKML-SLEAENT----------HLIKWEFHDLLFHSRSRKGRHSNPV 223 Query: 241 GGAFTHAGVVPPLPAVRPPWPGKKIDLGK---FSTSGSVSHFAKLLRERHSIRDFDDQHP 297 GG + + PLP V+PP + I L K + + ++L R SIR++++ +P Sbjct: 224 GGTYRFLEKMEPLPVVKPPMSEQVIQLYKPNLEELAKTDISLTQVLESRRSIREYNE-NP 282 Query: 298 VTLAELAQFLDTTARVLSEWKIGADFDGVPEATYSARPYPSAGGAYELELYLAVLNCGEL 357 +T+ +L + L AR I D + S RPYP G YELE+Y A+ C L Sbjct: 283 ITVEQLGELLYRCAR------IKEVIDLKEKVQVSRRPYPGGGAIYELEIYPAINYCQGL 336 Query: 358 ARGLYHYDAGSHALVAIDTSAQQLQALSTAAEFAMDASGPPQVLITIAARFGRISWKYSS 417 LYHY +H L + Q ++ L T A A G PQ+L+ I ARFGR WKY S Sbjct: 337 EAALYHYRPDAHELELVTQWNQGIETLFTDAYNASAQHGMPQILLIITARFGRFFWKYQS 396 Query: 418 IAYSLILKDVGNLMQTFYLTATDMGLGGCAIGTCNIDLFAKMTGLEFHVESPVGQFALG 476 +AY+ ILK VG + QT YL T M L CA+G+ N D+FAK TG++++ ES VG+F LG Sbjct: 397 MAYAAILKHVGVMYQTLYLVTTAMKLAPCALGSGNSDIFAKATGIDYYEESSVGEFMLG 455