BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0075.0011.p (559 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_08632801.1| AMP-dependent synthetase and ligase [Acidiphi... 507 e-141 ref|YP_001233802.1| AMP-dependent synthetase and ligase [Acidiph... 507 e-141 ref|YP_004283436.1| long-chain fatty-acid--CoA ligase [Acidiphil... 506 e-141 ref|YP_586588.1| long-chain-fatty-acid--CoA ligase [Cupriavidus ... 491 e-136 ref|YP_001105609.1| AMP-dependent Acyl-CoA synthetase and ligase... 468 e-129 >ref|ZP_08632801.1| AMP-dependent synthetase and ligase [Acidiphilium sp. PM] gb|EGO95419.1| AMP-dependent synthetase and ligase [Acidiphilium sp. PM] Length = 548 Score = 507 bits (1306), Expect = e-141, Method: Compositional matrix adjust. Identities = 273/523 (52%), Positives = 331/523 (63%), Gaps = 12/523 (2%) Query: 40 ALDLFRAA---PREAPALFYFDATQTYADIDRLSDRLAARFAQKGVGRGDRVAVILQNVP 96 AL +FRAA +APA+ YFD +YA++DRLSD LA A G G GDR+AV LQNVP Sbjct: 28 ALAMFRAAVARAPQAPAILYFDGRISYAELDRLSDGLAGYLAAHGFGAGDRLAVYLQNVP 87 Query: 97 QFVIVSVAAWKVGAIVVSLNPMYRTPELRKLFGDCEPKAVICHDDQWENVFPAAG-SVDS 155 QFVI + AWK G I V++NPM R E+ LF DC PKAV+CHD +E A + Sbjct: 88 QFVIAILGAWKAGGIAVAVNPMNRAREIGILFADCTPKAVVCHDTTFEETVGALEPGLRP 147 Query: 156 ELVFWTGAREFQTRNDVRVLPVPGAAPPAQALATVFAENAPA-----PPAPVLTSDDIAL 210 ++V T A FQTRND R+ A TV E A A PPAP DDIA Sbjct: 148 QIVLTTSALAFQTRNDRRLF---AATQRVACAGTVDFEAAIAAGGALPPAPSYKPDDIAF 204 Query: 211 LLYTSGTTGVPKGAMLTHRNLVATALICCDHFELNGTSRIFGVAPLFHITGFEIQMVAAF 270 L+YTSGTTGVPKGAM TH N+ A D L + I G+APLFHITG + AAF Sbjct: 205 LVYTSGTTGVPKGAMNTHGNVTFNAQTYRDWIGLGEGAPILGIAPLFHITGLIGHIAAAF 264 Query: 271 AARAAMVLTYRFQPQVALDAFLEWRPTFIVGAITAYIALMNQADATSRHFESFVHIYSGG 330 + ++L YRF+P V LDA E + F +GAITA+IA+MN DA S IYSGG Sbjct: 265 ITASPLILAYRFEPGVMLDAIEEHKAAFTIGAITAFIAMMNHPDAAPAKLASMTRIYSGG 324 Query: 331 APIAPSVIDAFARRFGRAIRSSYGMTELTSASHLAPNEGRIPVDPDSGALSIGRPTPAVD 390 API PSV+ AF FG I + YG+TE S +H+ P PVDP SGALSIG P Sbjct: 325 APIPPSVVTAFRETFGHYIHNGYGLTETNSPTHVVPLSREAPVDPASGALSIGVPAYNTI 384 Query: 391 AIVVDDERRPLGPGAHGEVVVRGPGVMAGYWRKPAETDEVLTNGWLHSGDVGFFDESGWF 450 + + DDE R L G GE+V +GP ++ GYW KP +T E + NG H+GDVGF D+ GWF Sbjct: 385 SWICDDEGRELPVGEVGEIVSQGPMIVPGYWNKPDKTAEAIRNGRFHTGDVGFMDKDGWF 444 Query: 451 YLVDRKKDMISASGFKVWPREVEDVIYGFPGVREAAVVGAADPYRGETVVAFVSAQPGTV 510 YLVDRKKDMI+A+G+KVWPREVEDV+Y P VREAAVVG AD YRGETV A +S +PGT Sbjct: 445 YLVDRKKDMINAAGYKVWPREVEDVLYTHPAVREAAVVGVADSYRGETVKAVISLRPGTS 504 Query: 511 IDAAALSRFCRERLAAYKCPVDIRVLDDLPKTESGKITRNTLR 553 + FC+ER+AAYK P + ++DDLPKT +GKI R LR Sbjct: 505 ATQEEIIAFCKERMAAYKYPRTVEIIDDLPKTVTGKILRRELR 547 >ref|YP_001233802.1| AMP-dependent synthetase and ligase [Acidiphilium cryptum JF-5] gb|ABQ29883.1| AMP-dependent synthetase and ligase [Acidiphilium cryptum JF-5] Length = 548 Score = 507 bits (1305), Expect = e-141, Method: Compositional matrix adjust. Identities = 273/523 (52%), Positives = 331/523 (63%), Gaps = 12/523 (2%) Query: 40 ALDLFRAA---PREAPALFYFDATQTYADIDRLSDRLAARFAQKGVGRGDRVAVILQNVP 96 AL +FRAA +APA+ YFD +YA++DRLSD LA A G G GDR+AV LQNVP Sbjct: 28 ALAMFRAAVARAPQAPAILYFDGRISYAELDRLSDGLAGYLAAHGFGAGDRLAVYLQNVP 87 Query: 97 QFVIVSVAAWKVGAIVVSLNPMYRTPELRKLFGDCEPKAVICHDDQWENVFPAAG-SVDS 155 QFVI + AWK G I V++NPM R E+ LF DC PKAV+CHD +E A + Sbjct: 88 QFVIAILGAWKAGGIAVAVNPMNRAREIGILFADCTPKAVVCHDTTFEETVGALEPGLRP 147 Query: 156 ELVFWTGAREFQTRNDVRVLPVPGAAPPAQALATVFAENAPA-----PPAPVLTSDDIAL 210 ++V T A FQTRND R+ A TV E A A PPAP DDIA Sbjct: 148 QIVLTTSALAFQTRNDRRLF---AATRRVACAGTVDFEAAIAAGGALPPAPSYKPDDIAF 204 Query: 211 LLYTSGTTGVPKGAMLTHRNLVATALICCDHFELNGTSRIFGVAPLFHITGFEIQMVAAF 270 L+YTSGTTGVPKGAM TH N+ A D L + I G+APLFHITG + AAF Sbjct: 205 LVYTSGTTGVPKGAMNTHGNVTFNAQTYRDWIGLGEGAPILGIAPLFHITGLIGHIAAAF 264 Query: 271 AARAAMVLTYRFQPQVALDAFLEWRPTFIVGAITAYIALMNQADATSRHFESFVHIYSGG 330 + ++L YRF+P V LDA E + F +GAITA+IA+MN DA S IYSGG Sbjct: 265 ITASPLILAYRFEPGVMLDAIEEHKAAFTIGAITAFIAMMNHPDAAPAKLASMTRIYSGG 324 Query: 331 APIAPSVIDAFARRFGRAIRSSYGMTELTSASHLAPNEGRIPVDPDSGALSIGRPTPAVD 390 API PSV+ AF FG I + YG+TE S +H+ P PVDP SGALSIG P Sbjct: 325 APIPPSVVTAFRETFGHYIHNGYGLTETNSPTHVVPLSREAPVDPASGALSIGVPAYNTI 384 Query: 391 AIVVDDERRPLGPGAHGEVVVRGPGVMAGYWRKPAETDEVLTNGWLHSGDVGFFDESGWF 450 + + DDE R L G GE+V +GP ++ GYW KP +T E + NG H+GDVGF D+ GWF Sbjct: 385 SWICDDEGRELPVGEVGEIVSQGPMIVPGYWNKPDKTAEAIRNGRFHTGDVGFMDKDGWF 444 Query: 451 YLVDRKKDMISASGFKVWPREVEDVIYGFPGVREAAVVGAADPYRGETVVAFVSAQPGTV 510 YLVDRKKDMI+A+G+KVWPREVEDV+Y P VREAAVVG AD YRGETV A +S +PGT Sbjct: 445 YLVDRKKDMINAAGYKVWPREVEDVLYTHPAVREAAVVGVADSYRGETVKAVISLRPGTS 504 Query: 511 IDAAALSRFCRERLAAYKCPVDIRVLDDLPKTESGKITRNTLR 553 + FC+ER+AAYK P + ++DDLPKT +GKI R LR Sbjct: 505 ATQEEIIAFCKERMAAYKYPRTVEIIDDLPKTVTGKILRRELR 547 >ref|YP_004283436.1| long-chain fatty-acid--CoA ligase [Acidiphilium multivorum AIU301] dbj|BAJ80554.1| long-chain fatty-acid--CoA ligase [Acidiphilium multivorum AIU301] Length = 548 Score = 506 bits (1303), Expect = e-141, Method: Compositional matrix adjust. Identities = 273/523 (52%), Positives = 331/523 (63%), Gaps = 12/523 (2%) Query: 40 ALDLFRAA---PREAPALFYFDATQTYADIDRLSDRLAARFAQKGVGRGDRVAVILQNVP 96 AL +FRAA +APA+ YFD +YA++DRLSD LA A G G GDR+AV LQNVP Sbjct: 28 ALVMFRAAVARAPQAPAILYFDGRISYAELDRLSDGLAGYLAAHGFGAGDRLAVYLQNVP 87 Query: 97 QFVIVSVAAWKVGAIVVSLNPMYRTPELRKLFGDCEPKAVICHDDQWENVFPAAG-SVDS 155 QFVI + AWK G I V++NPM R E+ LF DC PKAV+CHD +E A + Sbjct: 88 QFVIAILGAWKAGGIAVAVNPMNRAREIGILFADCTPKAVVCHDTTFEETVGALEPGLRP 147 Query: 156 ELVFWTGAREFQTRNDVRVLPVPGAAPPAQALATVFAENAPA-----PPAPVLTSDDIAL 210 ++V T A FQTRND R+ A TV E A A PPAP DDIA Sbjct: 148 QIVLTTSALAFQTRNDRRLF---AATRRVACAGTVDFEAAIAAGGALPPAPSYKPDDIAF 204 Query: 211 LLYTSGTTGVPKGAMLTHRNLVATALICCDHFELNGTSRIFGVAPLFHITGFEIQMVAAF 270 L+YTSGTTGVPKGAM TH N+ A D L + I G+APLFHITG + AAF Sbjct: 205 LVYTSGTTGVPKGAMNTHGNVTFNAQTYRDWIGLGEGAPILGIAPLFHITGLIGHIAAAF 264 Query: 271 AARAAMVLTYRFQPQVALDAFLEWRPTFIVGAITAYIALMNQADATSRHFESFVHIYSGG 330 + ++L YRF+P V LDA E + F +GAITA+IA+MN DA S IYSGG Sbjct: 265 ITASPLILAYRFEPGVMLDAIEEHKAAFTIGAITAFIAMMNHPDAAPAKLASMTRIYSGG 324 Query: 331 APIAPSVIDAFARRFGRAIRSSYGMTELTSASHLAPNEGRIPVDPDSGALSIGRPTPAVD 390 API PSV+ AF FG I + YG+TE S +H+ P PVDP SGALSIG P Sbjct: 325 APIPPSVVTAFRETFGHYIHNGYGLTETNSPTHVVPLSREAPVDPASGALSIGVPAYNTI 384 Query: 391 AIVVDDERRPLGPGAHGEVVVRGPGVMAGYWRKPAETDEVLTNGWLHSGDVGFFDESGWF 450 + + DDE R L G GE+V +GP ++ GYW KP +T E + NG H+GDVGF D+ GWF Sbjct: 385 SWICDDEGRELPVGEVGEIVSQGPMIVPGYWNKPDKTAEAIRNGRFHTGDVGFMDKDGWF 444 Query: 451 YLVDRKKDMISASGFKVWPREVEDVIYGFPGVREAAVVGAADPYRGETVVAFVSAQPGTV 510 YLVDRKKDMI+A+G+KVWPREVEDV+Y P VREAAVVG AD YRGETV A +S +PGT Sbjct: 445 YLVDRKKDMINAAGYKVWPREVEDVLYTHPAVREAAVVGVADSYRGETVKAVISLRPGTS 504 Query: 511 IDAAALSRFCRERLAAYKCPVDIRVLDDLPKTESGKITRNTLR 553 + FC+ER+AAYK P + ++DDLPKT +GKI R LR Sbjct: 505 ATQEEIIAFCKERMAAYKYPRTVEIIDDLPKTVTGKILRRELR 547 >ref|YP_586588.1| long-chain-fatty-acid--CoA ligase [Cupriavidus metallidurans CH34] gb|ABF11319.1| Long-chain-fatty-acid-CoA ligase (AMP-dependent synthetase and ligase) [Cupriavidus metallidurans CH34] Length = 555 Score = 491 bits (1263), Expect = e-136, Method: Compositional matrix adjust. Identities = 260/527 (49%), Positives = 337/527 (63%), Gaps = 13/527 (2%) Query: 40 ALDLFRAAPREAP---ALFYFDATQTYADIDRLSDRLAARFAQKGVGRGDRVAVILQNVP 96 AL LFRAA AP A+ YFD + +YA++D SD LA + +G+ RG+RVA+ LQN+P Sbjct: 28 ALSLFRAAVARAPSLPAILYFDGSLSYAELDTQSDALACAWQDRGITRGERVAIYLQNIP 87 Query: 97 QFVIVSVAAWKVGAIVVSLNPMYRTPELRKLFGDCEPKAVICHDDQWENVFPA--AGSVD 154 QFVI VA WK+GAI V +NPM R ELR + D + ++C + + ++ A + Sbjct: 88 QFVIALVATWKLGAIAVPVNPMNRARELRVILADSGARLLVCSETLYVDIVRPLLAEEAN 147 Query: 155 SE-----LVFWTGAREFQTRNDVRVLPVPG--AAPPAQALATVFAENAP-APPAPVLTSD 206 +E LV T E+Q R+D R+ P A++L+ V A NA PP L + Sbjct: 148 AENGVLPLVMTTSELEYQRRHDPRLFANTSRQETPGAESLSAVIAANAGRHPPPAALGPN 207 Query: 207 DIALLLYTSGTTGVPKGAMLTHRNLVATALICCDHFELNGTSRIFGVAPLFHITGFEIQM 266 DIA+L+YTSGTTGVPKGAM TH N+ A + D L I GVAPLFHITG + Sbjct: 208 DIAMLVYTSGTTGVPKGAMNTHGNVAFNAQVYRDWITLGEGEPILGVAPLFHITGLIGHI 267 Query: 267 VAAFAARAAMVLTYRFQPQVALDAFLEWRPTFIVGAITAYIALMNQADATSRHFESFVHI 326 AAF A +VL RF+P V LD E RP F +GAIT +IALMN +AT F S I Sbjct: 268 GAAFMCAAPLVLACRFEPGVMLDTLREHRPAFTIGAITVFIALMNHPNATRDCFASLTKI 327 Query: 327 YSGGAPIAPSVIDAFARRFGRAIRSSYGMTELTSASHLAPNEGRIPVDPDSGALSIGRPT 386 YSGGAPI PSV++AF RFG I + YG+TE S +H+ P PVDP SG L++G P Sbjct: 328 YSGGAPIPPSVVEAFRERFGHYIHNGYGLTETNSPTHVVPRGVEAPVDPASGTLAVGVPA 387 Query: 387 PAVDAIVVDDERRPLGPGAHGEVVVRGPGVMAGYWRKPAETDEVLTNGWLHSGDVGFFDE 446 V++ + DDE +P+ G GE++ RGP ++ GYW KP E+ + + +G+ +GDVGF DE Sbjct: 388 YNVESYIGDDEGKPMAVGEVGEIISRGPMIVPGYWNKPQESAKAIVDGYFRTGDVGFMDE 447 Query: 447 SGWFYLVDRKKDMISASGFKVWPREVEDVIYGFPGVREAAVVGAADPYRGETVVAFVSAQ 506 GWFYLVDRKKDMI+ASG+KVWPREVEDV+Y P VREAAVVG D YRGETV A VS + Sbjct: 448 RGWFYLVDRKKDMINASGYKVWPREVEDVLYTHPAVREAAVVGVPDAYRGETVKAVVSLK 507 Query: 507 PGTVIDAAALSRFCRERLAAYKCPVDIRVLDDLPKTESGKITRNTLR 553 PGT + L+ +C+ R+AAYK P + ++D+LPKT +GKI R LR Sbjct: 508 PGTNVTPEELAEYCKARMAAYKYPRVVMIMDELPKTVTGKILRRELR 554 >ref|YP_001105609.1| AMP-dependent Acyl-CoA synthetase and ligase [Saccharopolyspora erythraea NRRL 2338] ref|ZP_06561032.1| AMP-dependent Acyl-CoA synthetase and ligase [Saccharopolyspora erythraea NRRL 2338] emb|CAM02684.1| AMP-dependent Acyl-CoA synthetase and ligase [Saccharopolyspora erythraea NRRL 2338] Length = 550 Score = 468 bits (1205), Expect = e-129, Method: Compositional matrix adjust. Identities = 259/529 (48%), Positives = 318/529 (60%), Gaps = 11/529 (2%) Query: 34 DPGRRTALDLFRAAPREAP---ALFYFDATQTYADIDRLSDRLAARFAQKGVGRGDRVAV 90 +P AL++F A+ R AP A+ YFD + +++D LSD A +G+ RGDRVA+ Sbjct: 22 EPEFANALEMFAASVRAAPDRPAVKYFDGVLSRSELDELSDAFACGLLDRGIRRGDRVAL 81 Query: 91 ILQNVPQFVIVSVAAWKVGAIVVSLNPMYRTPELRKLFGDCEPKAVICHDDQWENVF-PA 149 LQNVPQF++ + WK G + V++NPM + EL L D +A++C + V P Sbjct: 82 FLQNVPQFLVALLGTWKAGCVAVAINPMNKQRELSLLLRDSGARALVCLESLHGPVAGPV 141 Query: 150 AGSVDSELVFWTGAREFQTRNDVRVLP-----VPGAAPPAQALATVFAENAPAPPAPVLT 204 G D ELV T E Q+R+D RVL A AL F PPA L Sbjct: 142 LGETDVELVMTTSELEHQSRDDPRVLGDVRRIRCEGAEDMSALIERF--RGRRPPAVELG 199 Query: 205 SDDIALLLYTSGTTGVPKGAMLTHRNLVATALICCDHFELNGTSRIFGVAPLFHITGFEI 264 DD+A+L YTSGTTG PKGAM THRNLV A D + + +FGVAPLFHITG Sbjct: 200 PDDVAVLTYTSGTTGPPKGAMNTHRNLVFNARAVRDWVGIGESDVVFGVAPLFHITGMVA 259 Query: 265 QMVAAFAARAAMVLTYRFQPQVALDAFLEWRPTFIVGAITAYIALMNQADATSRHFESFV 324 A A +VL RF+P A+D E RPTF +G+IT +IALMN +ATS S Sbjct: 260 HAALALLTTAPLVLFCRFEPNAAVDLIREHRPTFTIGSITVFIALMNAPEATSADLSSLT 319 Query: 325 HIYSGGAPIAPSVIDAFARRFGRAIRSSYGMTELTSASHLAPNEGRIPVDPDSGALSIGR 384 +YSGGAPI PS AF RFG I + YG+TE T+ +H P G PVDP+SGALSIG Sbjct: 320 RVYSGGAPIPPSTTRAFQERFGHYIHNIYGLTETTAPTHAVPLHGEAPVDPESGALSIGV 379 Query: 385 PTPAVDAIVVDDERRPLGPGAHGEVVVRGPGVMAGYWRKPAETDEVLTNGWLHSGDVGFF 444 P + A +V D+ L G GEVVVRGP V+AGYW KP ET L GWLH+GDVG+ Sbjct: 380 PMYSTVARIVGDDGADLPAGRIGEVVVRGPQVVAGYWNKPEETAGALPGGWLHTGDVGYM 439 Query: 445 DESGWFYLVDRKKDMISASGFKVWPREVEDVIYGFPGVREAAVVGAADPYRGETVVAFVS 504 D GWFYLVDRKKD I+ G+KVWPREVEDV+Y VREAAVVG D YRGE V FVS Sbjct: 440 DHVGWFYLVDRKKDQINTGGYKVWPREVEDVLYEHEAVREAAVVGVPDEYRGEMVKGFVS 499 Query: 505 AQPGTVIDAAALSRFCRERLAAYKCPVDIRVLDDLPKTESGKITRNTLR 553 +PG L FCR R+AAYK P + VL++LPKT +GK+ R LR Sbjct: 500 LRPGLTASPEELVDFCRRRMAAYKYPRQVEVLEELPKTATGKLLRRQLR 548