BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0072.0052.p (427 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_772678.1| transport protein [Bradyrhizobium japonicum USD... 736 0.0 ref|YP_002363659.1| major facilitator superfamily protein [Methy... 673 0.0 ref|YP_001834571.1| major facilitator transporter [Beijerinckia ... 624 e-176 ref|YP_002498027.1| major facilitator superfamily protein [Methy... 564 e-158 ref|YP_001755582.1| major facilitator transporter [Methylobacter... 558 e-157 >ref|NP_772678.1| transport protein [Bradyrhizobium japonicum USDA 110] dbj|BAC51303.1| hypothetical transport protein [Bradyrhizobium japonicum USDA 110] Length = 427 Score = 736 bits (1900), Expect = 0.0, Method: Compositional matrix adjust. Identities = 386/427 (90%), Positives = 405/427 (94%) Query: 1 MRLRFKATHVVLAMLCVMYFITYVDRVNIGTAAGEIQKELGLSNTELGLVFSAFAYPYLL 60 M +RFKATHVVLAMLC MYFITYVDRVNIGTAAGEIQKELGLSNT+LGLVFSAFAYPYLL Sbjct: 1 MGVRFKATHVVLAMLCAMYFITYVDRVNIGTAAGEIQKELGLSNTQLGLVFSAFAYPYLL 60 Query: 61 FQVIGGWVGDRFGPRQTLFWCGMIWAAATIMTGFVHGLAALFVARFALGFGEGATFPTAT 120 FQVIGGWVGD FGPR+TLFWCGMIWAAATIMTGFV+GLAALFVARFALGFGEGATFPTAT Sbjct: 61 FQVIGGWVGDHFGPRKTLFWCGMIWAAATIMTGFVNGLAALFVARFALGFGEGATFPTAT 120 Query: 121 RAMQYWTPANRRGFAQGLTHSFARLGNAITPPVVALLILWLTWRGAFVVLGVVSLIWGIA 180 RAMQYWTP NRRGFAQGLTHSFARLGNA+TPPVVALLILWLTWRGAFVVLG VSLIWG+ Sbjct: 121 RAMQYWTPTNRRGFAQGLTHSFARLGNAVTPPVVALLILWLTWRGAFVVLGFVSLIWGVI 180 Query: 181 WVWYFRNEPRDHGSITAAELATLPPRPQGERPKVPWGPLLQRMWPVTLTYFCYGWCLWLY 240 WVWYFRNEP++H SIT AELA LPPRP GERP+VPWGPLL RMWPVTLTYFCYGWCLWLY Sbjct: 181 WVWYFRNEPKEHASITEAELAALPPRPTGERPRVPWGPLLGRMWPVTLTYFCYGWCLWLY 240 Query: 241 LNWLPLFFKNNYSLDLKNSALFASGVFFAGVVGDSIGGVISDRILERTGNVRLARLSVTV 300 LNWLPLFFKNNYSLD+KNSALFASGVFFAGVVGDS+GGV+SD+IL+RTGNVRLARLSVTV Sbjct: 241 LNWLPLFFKNNYSLDIKNSALFASGVFFAGVVGDSVGGVLSDKILDRTGNVRLARLSVTV 300 Query: 301 VGFAGALLSLMPILFVHDITVVALCLSAGFFCAELVIGPMWSIPMDIAAKYSGTAAGIMN 360 GF GALLSL PILFVHDIT+VALCLSAGFFCAELVIGPMWSIPMDIA KYSGTA+GIMN Sbjct: 301 AGFTGALLSLFPILFVHDITIVALCLSAGFFCAELVIGPMWSIPMDIAPKYSGTASGIMN 360 Query: 361 TGSAFAAIVSPLVAGFVIDATGNWYLPFLMSMGLLLVGGFSAFLMHPERPFEETGELVAS 420 TGSAFAAIVSPLVAGFVID TGNWYLPFLMSMGLLL+GGFSAFLMHPERPF E + S Sbjct: 361 TGSAFAAIVSPLVAGFVIDVTGNWYLPFLMSMGLLLLGGFSAFLMHPERPFAEAEGRLPS 420 Query: 421 GKVVAAE 427 G+VVAAE Sbjct: 421 GRVVAAE 427 >ref|YP_002363659.1| major facilitator superfamily protein [Methylocella silvestris BL2] gb|ACK52297.1| major facilitator superfamily MFS_1 [Methylocella silvestris BL2] Length = 426 Score = 673 bits (1736), Expect = 0.0, Method: Compositional matrix adjust. Identities = 323/424 (76%), Positives = 371/424 (87%) Query: 4 RFKATHVVLAMLCVMYFITYVDRVNIGTAAGEIQKELGLSNTELGLVFSAFAYPYLLFQV 63 +FKATHVVLA+LC+MYFITY+DRVNIGTAAG IQ+EL L+NT+LGLVFSAFAYPYL+FQV Sbjct: 3 KFKATHVVLALLCIMYFITYIDRVNIGTAAGAIQRELNLTNTQLGLVFSAFAYPYLVFQV 62 Query: 64 IGGWVGDRFGPRQTLFWCGMIWAAATIMTGFVHGLAALFVARFALGFGEGATFPTATRAM 123 GGWVGDRFG R+TLF CG+IWA ATI+TG V+GL LF+AR LGFGEGATFPTATRAM Sbjct: 63 FGGWVGDRFGARRTLFACGLIWAVATILTGLVNGLFTLFLARLLLGFGEGATFPTATRAM 122 Query: 124 QYWTPANRRGFAQGLTHSFARLGNAITPPVVALLILWLTWRGAFVVLGVVSLIWGIAWVW 183 QYWTP +RRGFAQGLTH+FARLGNA+TPP VALL++ +WRG+FV+LG++SL+W + WVW Sbjct: 123 QYWTPPSRRGFAQGLTHAFARLGNAVTPPFVALLVIAFSWRGSFVLLGLISLVWVLVWVW 182 Query: 184 YFRNEPRDHGSITAAELATLPPRPQGERPKVPWGPLLQRMWPVTLTYFCYGWCLWLYLNW 243 YFRN+P+DH IT ELA LP R G + ++PW PLL RMWPVTLTYFCYGW LWLYLNW Sbjct: 183 YFRNDPKDHSGITEEELAQLPVRKPGAKLQIPWKPLLARMWPVTLTYFCYGWTLWLYLNW 242 Query: 244 LPLFFKNNYSLDLKNSALFASGVFFAGVVGDSIGGVISDRILERTGNVRLARLSVTVVGF 303 LPLFFKNN+ LDLK+SALFASGVFFAGVVGD++GGVISD IL RT NVRLARLSVT+ GF Sbjct: 243 LPLFFKNNFGLDLKSSALFASGVFFAGVVGDTLGGVISDHILRRTNNVRLARLSVTIGGF 302 Query: 304 AGALLSLMPILFVHDITVVALCLSAGFFCAELVIGPMWSIPMDIAAKYSGTAAGIMNTGS 363 GA LSL+P+L++HD+T+VALCLSAGFF AELVIGPMWSIPMDIA KYSGTAAG+MNTGS Sbjct: 303 LGAFLSLLPLLYIHDVTLVALCLSAGFFFAELVIGPMWSIPMDIAPKYSGTAAGLMNTGS 362 Query: 364 AFAAIVSPLVAGFVIDATGNWYLPFLMSMGLLLVGGFSAFLMHPERPFEETGELVASGKV 423 A AAIVSPLVAGFVID T NW+LPFLMSMGLLLVG AF+MHPE+PFEE + + K+ Sbjct: 363 AAAAIVSPLVAGFVIDVTANWFLPFLMSMGLLLVGAACAFMMHPEKPFEEHEMVGGTAKL 422 Query: 424 VAAE 427 AE Sbjct: 423 APAE 426 >ref|YP_001834571.1| major facilitator transporter [Beijerinckia indica subsp. indica ATCC 9039] gb|ACB97082.1| major facilitator superfamily MFS_1 [Beijerinckia indica subsp. indica ATCC 9039] Length = 428 Score = 624 bits (1608), Expect = e-176, Method: Compositional matrix adjust. Identities = 301/428 (70%), Positives = 355/428 (82%), Gaps = 1/428 (0%) Query: 1 MRLRFKATHVVLAMLCVMYFITYVDRVNIGTAAGEIQKELGLSNTELGLVFSAFAYPYLL 60 M + KATH VLA+LCVMYF+TYVDRVNIGTAA IQ++LGL+N +LG VFSAFAYPYLL Sbjct: 1 MGFKPKATHGVLALLCVMYFLTYVDRVNIGTAASGIQRDLGLTNIQLGFVFSAFAYPYLL 60 Query: 61 FQVIGGWVGDRFGPRQTLFWCGMIWAAATIMTGFVHGLAALFVARFALGFGEGATFPTAT 120 FQ+ GGW+ D GPRQTLFWCG+ WA ATI+TGF GL +L +AR LGFGEGATFPTAT Sbjct: 61 FQIFGGWICDHCGPRQTLFWCGVTWAIATILTGFAGGLTSLVLARLLLGFGEGATFPTAT 120 Query: 121 RAMQYWTPANRRGFAQGLTHSFARLGNAITPPVVALLILWLTWRGAFVVLGVVSLIWGIA 180 R+MQ W P +RGFAQGLTH+F+RLGNA+TPP+VA L++ TWRG+F++LGV+S IW +A Sbjct: 121 RSMQSWVPIGKRGFAQGLTHAFSRLGNAVTPPIVAALMIATTWRGSFILLGVLSFIWSLA 180 Query: 181 WVWYFRNEPRDHGSITAAELATLPPRPQGE-RPKVPWGPLLQRMWPVTLTYFCYGWCLWL 239 WVWYFRN+P+DH I AAEL TLP R + +PKVPWGPL ++MWPVTLTYFCYGWCLWL Sbjct: 181 WVWYFRNDPKDHPHIQAAELETLPVRTESAIKPKVPWGPLARQMWPVTLTYFCYGWCLWL 240 Query: 240 YLNWLPLFFKNNYSLDLKNSALFASGVFFAGVVGDSIGGVISDRILERTGNVRLARLSVT 299 YLNWLPLFFKNN++LD++NSALFA G+FFAGVVGD++GG+ISDRIL RTGN+RLARL V Sbjct: 241 YLNWLPLFFKNNFNLDIQNSALFAFGIFFAGVVGDTLGGLISDRILHRTGNLRLARLGVV 300 Query: 300 VVGFAGALLSLMPILFVHDITVVALCLSAGFFCAELVIGPMWSIPMDIAAKYSGTAAGIM 359 V GF GALLSL+P+ + D T+V LCL+AGFF AELVIGPMWSIPMDIA K+SGTAAG+M Sbjct: 301 VPGFVGALLSLLPLFVITDRTIVTLCLTAGFFFAELVIGPMWSIPMDIAPKFSGTAAGLM 360 Query: 360 NTGSAFAAIVSPLVAGFVIDATGNWYLPFLMSMGLLLVGGFSAFLMHPERPFEETGELVA 419 N GSA AAIVSPL G VID TGNW LPF +S+GLLL+G FSAFLMHPE+ + V Sbjct: 361 NIGSAAAAIVSPLAIGLVIDMTGNWKLPFNISIGLLLIGTFSAFLMHPEKQLTDKDPSVP 420 Query: 420 SGKVVAAE 427 K + AE Sbjct: 421 FAKPLPAE 428 >ref|YP_002498027.1| major facilitator superfamily protein [Methylobacterium nodulans ORS 2060] gb|ACL57724.1| major facilitator superfamily MFS_1 [Methylobacterium nodulans ORS 2060] Length = 429 Score = 564 bits (1453), Expect = e-158, Method: Compositional matrix adjust. Identities = 284/414 (68%), Positives = 340/414 (82%), Gaps = 1/414 (0%) Query: 1 MRLRFKATHVVLAMLCVMYFITYVDRVNIGTAAGEIQKELGLSNTELGLVFSAFAYPYLL 60 M++R KAT VL +LC+MYFITY+DRVNIGTAA I+++LGL+NT+LGL+FSAFAYPY + Sbjct: 1 MQVRVKATTSVLLLLCLMYFITYIDRVNIGTAASHIKQDLGLTNTQLGLIFSAFAYPYAV 60 Query: 61 FQVIGGWVGDRFGPRQTLFWCGMIWAAATIMTGFVHGLAALFVARFALGFGEGATFPTAT 120 FQVIGG +GDRFG R+TL CG+IWA+ATI+TGF G+A+LF+AR LGFGEGATFPTAT Sbjct: 61 FQVIGGSIGDRFGARRTLLLCGVIWASATILTGFAGGIASLFLARVMLGFGEGATFPTAT 120 Query: 121 RAMQYWTPANRRGFAQGLTHSFARLGNAITPPVVALLILWLTWRGAFVVLGVVSLIWGIA 180 RAMQ WT A+RRGFAQG+TH+FARLGNA+ PP+VALLI+ + WR +FVVLGV S +W IA Sbjct: 121 RAMQNWTAASRRGFAQGITHAFARLGNAVAPPIVALLIVLVGWRWSFVVLGVASFLWVIA 180 Query: 181 WVWYFRNEPRDHGSITAAELATL-PPRPQGERPKVPWGPLLQRMWPVTLTYFCYGWCLWL 239 W YFR++PRDH I+A ELA L PPR G R VPWG L +RM PVTLTYFCYGW LWL Sbjct: 181 WYGYFRDDPRDHARISAEELAALPPPRTPGSRSAVPWGRLTRRMLPVTLTYFCYGWTLWL 240 Query: 240 YLNWLPLFFKNNYSLDLKNSALFASGVFFAGVVGDSIGGVISDRILERTGNVRLARLSVT 299 +LNWLP FFK Y LDLKNSALFASGVFFAGVVGD++GGV+SD IL RTGN++ ARLSV Sbjct: 241 FLNWLPSFFKEGYGLDLKNSALFASGVFFAGVVGDTLGGVLSDHILTRTGNLKFARLSVI 300 Query: 300 VVGFAGALLSLMPILFVHDITVVALCLSAGFFCAELVIGPMWSIPMDIAAKYSGTAAGIM 359 + G GA SL+ + F D+T++A+ LSAGFF ELVIGP+WS+PMDIA +YSGTA+G+M Sbjct: 301 LFGMLGAAASLVGVFFTRDLTLIAIMLSAGFFFLELVIGPIWSVPMDIAPQYSGTASGLM 360 Query: 360 NTGSAFAAIVSPLVAGFVIDATGNWYLPFLMSMGLLLVGGFSAFLMHPERPFEE 413 NTGSA AAIVSPLV G+++D TGNW LPF S+GLLL G AF MHPER F + Sbjct: 361 NTGSAVAAIVSPLVFGWIVDLTGNWILPFAGSIGLLLAGAALAFTMHPERRFVD 414 >ref|YP_001755582.1| major facilitator transporter [Methylobacterium radiotolerans JCM 2831] gb|ACB24899.1| major facilitator superfamily MFS_1 [Methylobacterium radiotolerans JCM 2831] Length = 430 Score = 558 bits (1437), Expect = e-157, Method: Compositional matrix adjust. Identities = 274/414 (66%), Positives = 329/414 (79%), Gaps = 1/414 (0%) Query: 1 MRLRFKATHVVLAMLCVMYFITYVDRVNIGTAAGEIQKELGLSNTELGLVFSAFAYPYLL 60 M RF AT+ VL +LCVMYFITY+DRVNI TA EI ++ G+S T+ GL+ SAFAYPY + Sbjct: 1 MSKRFSATNGVLGLLCVMYFITYLDRVNIATAGAEIMRDFGMSKTDFGLILSAFAYPYAI 60 Query: 61 FQVIGGWVGDRFGPRQTLFWCGMIWAAATIMTGFVHGLAALFVARFALGFGEGATFPTAT 120 FQVIGG VGD+FG R+TL CG+IWA+ATI+TG V GL +LF+AR LGFGEGATFPTAT Sbjct: 61 FQVIGGSVGDKFGARRTLLACGVIWASATILTGMVGGLVSLFLARVLLGFGEGATFPTAT 120 Query: 121 RAMQYWTPANRRGFAQGLTHSFARLGNAITPPVVALLILWLTWRGAFVVLGVVSLIWGIA 180 RAMQ WT RRGFAQG+TH+FARLGNA+ PP+VA LI WR +FV+LG VS +W Sbjct: 121 RAMQNWTAPGRRGFAQGITHAFARLGNAVAPPIVAFLIYHFGWRTSFVILGAVSFVWVAI 180 Query: 181 WVWYFRNEPRDHGSITAAELATLPP-RPQGERPKVPWGPLLQRMWPVTLTYFCYGWCLWL 239 W YFR++P +H +IT AEL TLPP R G + VPWG L +RM PV++TYFCYGW LWL Sbjct: 181 WWLYFRDDPSEHPAITKAELDTLPPPRKVGAKQSVPWGALTRRMLPVSITYFCYGWTLWL 240 Query: 240 YLNWLPLFFKNNYSLDLKNSALFASGVFFAGVVGDSIGGVISDRILERTGNVRLARLSVT 299 +L WLP FFK NYSLDLKNSALFASGVFFAGVVGD++GG++SD IL RTGNV+LARLSV Sbjct: 241 FLGWLPSFFKENYSLDLKNSALFASGVFFAGVVGDTVGGMLSDSILHRTGNVKLARLSVI 300 Query: 300 VVGFAGALLSLMPILFVHDITVVALCLSAGFFCAELVIGPMWSIPMDIAAKYSGTAAGIM 359 V+GF GA SL + D+T+VAL LS+GFF AELVIGP+WS+PMDIA KY+GTA+G+M Sbjct: 301 VIGFLGAAASLAGVFMTRDLTLVALLLSSGFFFAELVIGPIWSVPMDIAPKYAGTASGLM 360 Query: 360 NTGSAFAAIVSPLVAGFVIDATGNWYLPFLMSMGLLLVGGFSAFLMHPERPFEE 413 NTGSA AAIVSP+V G ++DATG+W LPF+ S+GL L+G AF MHPER F + Sbjct: 361 NTGSAVAAIVSPIVFGVIVDATGSWTLPFVGSIGLCLLGAALAFTMHPEREFVD 414