BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0072.0032.p (448 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_317155.1| type I secretion membrane fusion protein, HlyD ... 710 0.0 ref|YP_001208835.1| HlyD family secretion protein [Bradyrhizobiu... 706 0.0 ref|YP_576360.1| Type I secretion membrane fusion protein, HlyD ... 701 0.0 ref|ZP_01044612.1| type I secretion membrane fusion protein, Hly... 698 0.0 gb|AAS83026.1| secretion protein-like protein [Azospirillum bras... 329 5e-88 >ref|YP_317155.1| type I secretion membrane fusion protein, HlyD [Nitrobacter winogradskyi Nb-255] gb|ABA03803.1| type I secretion membrane fusion protein, HlyD [Nitrobacter winogradskyi Nb-255] Length = 448 Score = 710 bits (1832), Expect = 0.0, Method: Compositional matrix adjust. Identities = 339/448 (75%), Positives = 387/448 (86%) Query: 1 LSPNQASLATVRQFQSETDAIREAPESMLARATLFVLSALLVSIVAIMCLTRLDRVITSV 60 +SP+QASLATVRQFQSETDAIREA E ++AR TLFVLSA LVSIVAI+CLT++DRVITS+ Sbjct: 1 MSPSQASLATVRQFQSETDAIREAAEPLVARVTLFVLSAFLVSIVAILCLTQVDRVITSL 60 Query: 61 RGKIVPVDQINVLQALDPSIIKTIDVREGEQVDTGQLLATLDSTFTAADVTQYKLQIASL 120 G IVPV +NVLQALDPSIIKTIDVREGEQV TGQLLATLD+TFT+AD+TQ KLQIASL Sbjct: 61 SGNIVPVGHVNVLQALDPSIIKTIDVREGEQVKTGQLLATLDATFTSADLTQAKLQIASL 120 Query: 121 DAQVARDEAELSGKPLSYGNAKDPDAARYNALQKALYDQRVAQYSAQVTSYDSKISQTEA 180 +AQ ARDEAEL +PL + + D D +Y ALQKALY+QRVAQY AQ+ S++SKI +T+A Sbjct: 121 EAQAARDEAELKQQPLVFPDNPDSDFQKYAALQKALYEQRVAQYQAQINSFESKIRETKA 180 Query: 181 TISKAKKDDERYGQRAEIAGKIEDMRSTLAESGSGSKLNLYLSQDTRLELLRQIDNTHNT 240 TI K + DD RY QR EI KIE MR+TLAE G+GS+LN+Y+SQD RLELLR ++N HN Sbjct: 181 TIEKLRADDTRYRQREEIMAKIEQMRTTLAERGTGSQLNMYISQDARLELLRTLENAHNG 240 Query: 241 LLEAQHQLDSLRADRKAFIEQWNAQLSQDLVTTRNNLDTARAAYDKAIKHQDLVRWTAAE 300 L+EAQ+ L S+ ADR AF +QW AQLSQDLVTTRN LD ARA Y+KA KHQDLVRWTAAE Sbjct: 241 LIEAQNTLGSITADRDAFKQQWFAQLSQDLVTTRNKLDEARATYEKAFKHQDLVRWTAAE 300 Query: 301 PSVVLTLAKLSVGSVLKPGDPFITLMPLKTRLEAEIQVLSRDVGFIRPNDPCTLKVDAFN 360 PSVVLT+AKLSVGSVLKPGDPFITLMP+ T+LEAEI++ SRDVGFIR DPC +K+DAFN Sbjct: 301 PSVVLTMAKLSVGSVLKPGDPFITLMPIDTKLEAEIRISSRDVGFIRAGDPCVMKIDAFN 360 Query: 361 AAEHGTAAGKIRWISEGAFTTDDDGKPVDAYYKARCSVDQSNFTNVPNNFRLIPGMTLQG 420 AAEHGTA GK+RWISEG FTTDDDGKPVDAYYKARCSVD S F VP+NFRLIPGMTLQG Sbjct: 361 AAEHGTAHGKVRWISEGTFTTDDDGKPVDAYYKARCSVDASAFKGVPSNFRLIPGMTLQG 420 Query: 421 DVNVGTRSVAMYLLSDMLRGMTEAMREP 448 D+NVGTRSVAMYLL ML+GM+EAMREP Sbjct: 421 DINVGTRSVAMYLLGGMLKGMSEAMREP 448 >ref|YP_001208835.1| HlyD family secretion protein [Bradyrhizobium sp. ORS 278] emb|CAL80620.1| Putative HlyD family secretion protein [Bradyrhizobium sp. ORS 278] Length = 458 Score = 706 bits (1823), Expect = 0.0, Method: Compositional matrix adjust. Identities = 338/447 (75%), Positives = 389/447 (87%) Query: 2 SPNQASLATVRQFQSETDAIREAPESMLARATLFVLSALLVSIVAIMCLTRLDRVITSVR 61 S + A++ATVRQFQSETDAIREAPE + ARATL+VL A+++S++A+MCL RLDRV+ SV Sbjct: 12 SRSPAAVATVRQFQSETDAIREAPEPVGARATLYVLVAMILSLIAVMCLMRLDRVVQSVG 71 Query: 62 GKIVPVDQINVLQALDPSIIKTIDVREGEQVDTGQLLATLDSTFTAADVTQYKLQIASLD 121 GKIVP+D INVLQALD SIIKTIDVREG+QV +GQLLATLD TFT ADV QYK Q+ASL Sbjct: 72 GKIVPLDAINVLQALDASIIKTIDVREGQQVASGQLLATLDPTFTQADVAQYKAQVASLS 131 Query: 122 AQVARDEAELSGKPLSYGNAKDPDAARYNALQKALYDQRVAQYSAQVTSYDSKISQTEAT 181 AQVARD+AEL+G PL + + D + A Y LQ ALY QR AQY AQ+ S+DSKISQTEAT Sbjct: 132 AQVARDKAELAGTPLVFPPSDDAEFASYAKLQLALYRQRRAQYDAQIASFDSKISQTEAT 191 Query: 182 ISKAKKDDERYGQRAEIAGKIEDMRSTLAESGSGSKLNLYLSQDTRLELLRQIDNTHNTL 241 I+K +KDDERYGQR ++ KIE MRSTLAESGSGSKLNLY+SQDTRLELLRQID+THN+L Sbjct: 192 IAKLQKDDERYGQRDDVLQKIETMRSTLAESGSGSKLNLYISQDTRLELLRQIDSTHNSL 251 Query: 242 LEAQHQLDSLRADRKAFIEQWNAQLSQDLVTTRNNLDTARAAYDKAIKHQDLVRWTAAEP 301 +EA+HQ++SLRADRKAFIEQWNAQLSQDLVTT+N+LD A AAYDKAI+HQDLVR +A EP Sbjct: 252 IEAKHQVESLRADRKAFIEQWNAQLSQDLVTTQNSLDAANAAYDKAIRHQDLVRLSAPEP 311 Query: 302 SVVLTLAKLSVGSVLKPGDPFITLMPLKTRLEAEIQVLSRDVGFIRPNDPCTLKVDAFNA 361 SVVLTLAKLSVGSVLKPGDPFITLMPL+ RLEAEI++ SRD+GF+RP DPCTLKVDAFN Sbjct: 312 SVVLTLAKLSVGSVLKPGDPFITLMPLRARLEAEIRIASRDIGFVRPGDPCTLKVDAFNH 371 Query: 362 AEHGTAAGKIRWISEGAFTTDDDGKPVDAYYKARCSVDQSNFTNVPNNFRLIPGMTLQGD 421 EHGTA G IRW+SEGAFT DDDGKPVDAYYKARCSVD +NF +VP FRLIPGMTL GD Sbjct: 372 VEHGTAQGTIRWVSEGAFTLDDDGKPVDAYYKARCSVDATNFHDVPAGFRLIPGMTLTGD 431 Query: 422 VNVGTRSVAMYLLSDMLRGMTEAMREP 448 +N+GTRS AMY+L ML G+ +AMREP Sbjct: 432 INIGTRSAAMYVLGSMLHGLGQAMREP 458 >ref|YP_576360.1| Type I secretion membrane fusion protein, HlyD [Nitrobacter hamburgensis X14] gb|ABE61900.1| Type I secretion membrane fusion protein, HlyD [Nitrobacter hamburgensis X14] Length = 449 Score = 701 bits (1808), Expect = 0.0, Method: Compositional matrix adjust. Identities = 335/449 (74%), Positives = 392/449 (87%), Gaps = 1/449 (0%) Query: 1 LSPNQASLATVRQFQSETDAIREAPESMLARATLFVLSALLVSIVAIMCLTRLDRVITSV 60 +SP+QASLATVRQFQSETDAIREA E ++ARATLFVLSA LVSIVAI+CLTR+DRVITS+ Sbjct: 1 MSPSQASLATVRQFQSETDAIREAAEPLVARATLFVLSAFLVSIVAILCLTRIDRVITSL 60 Query: 61 RGKIVPVDQINVLQALDPSIIKTIDVREGEQVDTGQLLATLDSTFTAADVTQYKLQIASL 120 G+IVPV +NVLQALDPSIIKTI+VREGEQV+TGQLLATLD+TFT+AD+TQ KLQ+ASL Sbjct: 61 SGRIVPVGHVNVLQALDPSIIKTINVREGEQVETGQLLATLDATFTSADLTQAKLQVASL 120 Query: 121 DAQVARDEAELSGKPLSYGNAKDPDAARYNALQKALYDQRVAQYSAQVTSYDSKISQTEA 180 +AQ ARDEAEL +PL + + DPD +Y ALQKALY QRVAQY+AQ+ S++SKI +T+A Sbjct: 121 EAQAARDEAELKQQPLVFADNPDPDFQKYAALQKALYGQRVAQYTAQLNSFESKIKETQA 180 Query: 181 TISKAKKDDERYGQRAEIAGKIEDMRSTLAESGSGSKLNLYLSQDTRLELLRQIDNTHNT 240 TI K + DD RY QR EI KIE MR+TLAE G+GS+LN+Y+SQD RLELLR ++N HN Sbjct: 181 TIEKLRDDDARYRQRDEILQKIETMRTTLAERGTGSRLNMYISQDARLELLRTLENAHNG 240 Query: 241 LLEAQHQLDSLRADRKAFIEQWNAQLSQDLVTTRNNLDTARAAYDKAIKHQDLVRWTAAE 300 L+EA++ L S+ ADR AF +QW AQLSQDLVTTRN LD ARA Y+KA+KHQ+LVRWTAA+ Sbjct: 241 LIEAKNTLGSITADRDAFKQQWFAQLSQDLVTTRNKLDEARATYEKALKHQNLVRWTAAD 300 Query: 301 PSVVLTLAKLSVGSVLKPGDPFITLMPLKTRLEAEIQVLSRDVGFIRPNDPCTLKVDAFN 360 PSVVLT+A+LSVGSVLKPGDPFITLMP+ T+LEAEI++ SRDVGFIR DPCT+KVDAFN Sbjct: 301 PSVVLTMARLSVGSVLKPGDPFITLMPIDTKLEAEIRISSRDVGFIRAGDPCTMKVDAFN 360 Query: 361 AAEHGTAAGKIRWISEGAFTTDDDGKPVD-AYYKARCSVDQSNFTNVPNNFRLIPGMTLQ 419 AA HGTA+G +RWISEGAFTTDDDGKP+D YYKARCSVD SNF +VP+NFRLIPGMTLQ Sbjct: 361 AAVHGTASGNVRWISEGAFTTDDDGKPLDYTYYKARCSVDASNFKDVPSNFRLIPGMTLQ 420 Query: 420 GDVNVGTRSVAMYLLSDMLRGMTEAMREP 448 GD+NVGTRSVAMYLL ML+G EAMREP Sbjct: 421 GDINVGTRSVAMYLLGGMLKGFHEAMREP 449 >ref|ZP_01044612.1| type I secretion membrane fusion protein, HlyD [Nitrobacter sp. Nb-311A] gb|EAQ37393.1| type I secretion membrane fusion protein, HlyD [Nitrobacter sp. Nb-311A] Length = 449 Score = 698 bits (1802), Expect = 0.0, Method: Compositional matrix adjust. Identities = 335/449 (74%), Positives = 388/449 (86%), Gaps = 1/449 (0%) Query: 1 LSPNQASLATVRQFQSETDAIREAPESMLARATLFVLSALLVSIVAIMCLTRLDRVITSV 60 +SP+QASLATVRQFQSETDAIREA E ++AR TLFVL+A L+SIVAI+CLTR+DRVITS+ Sbjct: 1 MSPSQASLATVRQFQSETDAIREAAEPLVARVTLFVLTAFLISIVAILCLTRVDRVITSL 60 Query: 61 RGKIVPVDQINVLQALDPSIIKTIDVREGEQVDTGQLLATLDSTFTAADVTQYKLQIASL 120 G IVPV +NVLQALDPSIIKTIDVREGEQV GQLLATLDSTFT+AD+TQ KLQIASL Sbjct: 61 NGNIVPVGHVNVLQALDPSIIKTIDVREGEQVKPGQLLATLDSTFTSADLTQAKLQIASL 120 Query: 121 DAQVARDEAELSGKPLSYGNAKDPDAARYNALQKALYDQRVAQYSAQVTSYDSKISQTEA 180 +AQ ARDEAEL +PL + + DPD +Y ALQ ALY QRVAQY AQ+ S++SKI +T+A Sbjct: 121 EAQAARDEAELKQQPLVFPDNPDPDFRKYAALQNALYKQRVAQYQAQLNSFESKIRETKA 180 Query: 181 TISKAKKDDERYGQRAEIAGKIEDMRSTLAESGSGSKLNLYLSQDTRLELLRQIDNTHNT 240 TI K + DD RY QR EI KIE+MR+TLAE G+GS+LN+Y+SQD RLELLR ++N HN Sbjct: 181 TIEKLRDDDARYRQRDEILAKIENMRTTLAERGTGSQLNMYISQDARLELLRTLENAHNG 240 Query: 241 LLEAQHQLDSLRADRKAFIEQWNAQLSQDLVTTRNNLDTARAAYDKAIKHQDLVRWTAAE 300 L+ AQ+ L ++ ADR AF EQW AQLSQDLVTTRN LD ARA Y+KA+KHQDLVRWTAAE Sbjct: 241 LIVAQNTLGAITADRDAFKEQWFAQLSQDLVTTRNKLDEARATYEKALKHQDLVRWTAAE 300 Query: 301 PSVVLTLAKLSVGSVLKPGDPFITLMPLKTRLEAEIQVLSRDVGFIRPNDPCTLKVDAFN 360 PSVVLT+A+LSVGSVLKPGDPFITLMP+ T+LEAEI++ SRDVGF+R DPCT+KVDAFN Sbjct: 301 PSVVLTIARLSVGSVLKPGDPFITLMPIDTKLEAEIRISSRDVGFVRAGDPCTMKVDAFN 360 Query: 361 AAEHGTAAGKIRWISEGAFTTDDDGKPVD-AYYKARCSVDQSNFTNVPNNFRLIPGMTLQ 419 AAEHGTA+GK+RWISEGAFTTDDDGKP++ YYKARCSVD SNF VP+NFRLIPGMTLQ Sbjct: 361 AAEHGTASGKVRWISEGAFTTDDDGKPLEYTYYKARCSVDASNFKGVPSNFRLIPGMTLQ 420 Query: 420 GDVNVGTRSVAMYLLSDMLRGMTEAMREP 448 GD+NVG RSVAMYLL ML+GM+EAMREP Sbjct: 421 GDINVGDRSVAMYLLGGMLKGMSEAMREP 449 >gb|AAS83026.1| secretion protein-like protein [Azospirillum brasilense] Length = 452 Score = 329 bits (844), Expect = 5e-88, Method: Compositional matrix adjust. Identities = 173/447 (38%), Positives = 279/447 (62%), Gaps = 7/447 (1%) Query: 3 PNQASLATVRQFQSETDAIREAPESMLARATLFVLSALLVSIVAIMCLTRLDRVITSVRG 62 P+ A+ + FQ ET I APE AR ++VL+A+++ + + + +LDRV+++ G Sbjct: 12 PDLAAEQAINDFQGETAEIIGAPEPFKARIVVWVLAAMVILFIVLASVMKLDRVVSAT-G 70 Query: 63 KIVPVDQINVLQALDPSIIKTIDVREGEQVDTGQLLATLDSTFTAADVTQYKLQIASLDA 122 ++V V+Q L+ SII++++VR G+ V G +LATLD TF+AADV Q Q++ L A Sbjct: 71 RVVSQAPTIVVQPLEISIIRSLNVRAGQTVRRGDVLATLDPTFSAADVAQLGRQVSKLTA 130 Query: 123 QVARDEAELSGKPLSYGNAKDPDAARYNALQKALYDQRVAQYSAQVTSYDSKISQTEATI 182 ++AR +AE +G P + KD D LQ++++ R A+Y+A++ ++D +++ +ATI Sbjct: 131 EIARLDAEAAGVPFTVAG-KDQDMV----LQESIWQYRQAEYAAKIANFDQRMATLQATI 185 Query: 183 SKAKKDDERYGQRAEIAGKIEDMRSTLAESGSGSKLNLYLSQDTRLELLRQIDNTHNTLL 242 A+ D E Y R +I G+IE+MR TL ++ +GS+LN ++ DTR+E R + + NT Sbjct: 186 KSARTDAEHYRSRLKIVGEIEEMRRTLEKNQTGSRLNSLIASDTRVETERNLGLSENTTR 245 Query: 243 EAQHQLDSLRADRKAFIEQWNAQLSQDLVTTRNNLDTARAAYDKAIKHQDLVRWTAAEPS 302 + H L++LRA+R+ F++QW + + DL T + L+ AR KA K +DLV A E + Sbjct: 246 TSTHDLEALRAEREVFVQQWRSTVLTDLSTRQVELERAREELAKAQKRRDLVELRAIEDA 305 Query: 303 VVLTLAKLSVGSVLKPGDPFITLMPLKTRLEAEIQVLSRDVGFIRPNDPCTLKVDAFNAA 362 VVL + K SVGSV++ P TLMPL +LE E ++ D GF++P D +K+DA+ Sbjct: 306 VVLEVGKYSVGSVVESAQPIYTLMPLNAKLEVEAEIAGIDQGFVKPGDTVQVKLDAYRFI 365 Query: 363 EHGTAAGKIRWISEGAFT-TDDDGKPVDAYYKARCSVDQSNFTNVPNNFRLIPGMTLQGD 421 EHG A G ++ ISE +FT +D + +++A+ + + + +VP++FRL+PGM L D Sbjct: 366 EHGMAKGVVKTISEDSFTRREDQSQAQRPFFRAKIELTEIDLRDVPSDFRLVPGMPLVAD 425 Query: 422 VNVGTRSVAMYLLSDMLRGMTEAMREP 448 + VGTR++ YL+ L+ E MREP Sbjct: 426 IVVGTRTIIAYLVEGALKSRAEGMREP 452