BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0067.0025.p (258 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_001770423.1| amidohydrolase [Methylobacterium sp. 4-46] >... 181 6e-44 ref|YP_001526809.1| cytosine deaminase [Azorhizobium caulinodans... 165 8e-39 ref|ZP_08551077.1| cytosine deaminase [Salinisphaera shabanensis... 158 1e-36 ref|YP_771060.1| putative amidohydrolase [Rhizobium leguminosaru... 92 6e-17 ref|ZP_08867720.1| cytosine deaminase [Azospirillum amazonense Y... 92 7e-17 >ref|YP_001770423.1| amidohydrolase [Methylobacterium sp. 4-46] gb|ACA17989.1| amidohydrolase [Methylobacterium sp. 4-46] Length = 491 Score = 181 bits (460), Expect = 6e-44, Method: Compositional matrix adjust. Identities = 108/176 (61%), Positives = 135/176 (76%), Gaps = 2/176 (1%) Query: 42 PAKGPRSFILPAFVNAHDHARPRA-SSFGAIGMPLESWILRTVVGTPVDAYLSAASAFAR 100 PA R ++PA VNAHDHARP + +SFGA +PLE+W+ R+ + TP DAYL+AA+ AR Sbjct: 51 PAVPGRRLVMPALVNAHDHARPLSLTSFGAAFLPLETWLPRSALATPPDAYLAAAAPLAR 110 Query: 101 AAQAGCAAMMVHYTRPSGTMPLVEEARAIARAAADVGIRIAFAIAVRDQNPIVYGDSEAV 160 AA+AGCAA+MVHYTRPSG +PL++EAR +ARAA DVG+R+ FA+AVRD+NP+VYG S+ V Sbjct: 111 AARAGCAAVMVHYTRPSGRLPLLDEAREVARAARDVGVRMTFALAVRDRNPLVYGASDEV 170 Query: 161 LSDLPAHDRKTIEQLFVRTPMSPQAYIELTDAI-XAIGGPKVDVQLGPAGVQWCSK 215 L DLPA R IE F+R SP Y+ LT+AI AI GP V VQ GPAGVQWCS+ Sbjct: 171 LDDLPAGTRAAIEAAFLRPAPSPAEYVALTEAIAAAIEGPTVAVQFGPAGVQWCSR 226 >ref|YP_001526809.1| cytosine deaminase [Azorhizobium caulinodans ORS 571] dbj|BAF89891.1| putative cytosine deaminase [Azorhizobium caulinodans ORS 571] Length = 481 Score = 165 bits (417), Expect = 8e-39, Method: Compositional matrix adjust. Identities = 91/184 (49%), Positives = 117/184 (63%), Gaps = 4/184 (2%) Query: 35 GISEAASPAKGPRS--FILPAFVNAHDHARP-RASSFGAIGMPLESWILRTVVGTPVDAY 91 G EA A+GP F++P VNAHDH RP R SS GA G PLE+WI + VD Y Sbjct: 31 GRIEALLAAQGPVDDLFVMPTPVNAHDHGRPVRTSSIGAGGKPLEAWIPYLALFPSVDPY 90 Query: 92 LSAASAFARAAQAGCAAMMVHYTRPSGTMPLVEEARAIARAAADVGIRIAFAIAVRDQNP 151 L+AA + + +A G +M+HYTR G L E R +ARAA DVG+R+ FAIA++D+NP Sbjct: 91 LAAALSLSHSALGGAGVVMMHYTRAQGFTDLPGEVREVARAARDVGVRVGFAIAMKDRNP 150 Query: 152 IVYGDSEAVLSDLPAHDRKTIEQLFVRTPMSPQAYIELTDAI-XAIGGPKVDVQLGPAGV 210 +VYG S +VL + R IE+ F+R + P+A+IEL DA+ A GGP DVQ GP G Sbjct: 151 LVYGPSASVLGGVAEPARSRIERQFLRPSLEPKAFIELADAVAEAAGGPDFDVQYGPNGP 210 Query: 211 QWCS 214 QWCS Sbjct: 211 QWCS 214 >ref|ZP_08551077.1| cytosine deaminase [Salinisphaera shabanensis E1L3A] gb|EGM33595.1| cytosine deaminase [Salinisphaera shabanensis E1L3A] Length = 479 Score = 158 bits (399), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 85/190 (44%), Positives = 112/190 (58%), Gaps = 2/190 (1%) Query: 28 HADGIITGISEAASPAKGPRSFILPAFVNAHDHARPRAS-SFGAIGMPLESWILRTVVGT 86 H DG IT I + GPR ++P F NAHDH RP + +FGA+ PLE+W+LR V Sbjct: 25 HDDGRITAIEPLGARPAGPRRLVMPCFANAHDHGRPLPTVAFGAVDKPLETWLLRLAVAP 84 Query: 87 PVDAYLSAASAFARAAQAGCAAMMVHYTRPSGTMPLVEEARAIARAAADVGIRIAFAIAV 146 VD Y++A ++F RAA G A+M+H TR G L E A+ARAA VGIR AFA+ + Sbjct: 85 SVDPYIAACASFGRAALGGSGAVMMHCTRAQGLNDLPTEVAAVARAADTVGIRAAFAVGL 144 Query: 147 RDQNPIVYGDSEAVLSDLPAHDRKTIEQLFVRTPMSPQAYIELTDAI-XAIGGPKVDVQL 205 RD+NP+VYGD + + + A R ++ F S A +E DA A+ +DVQ Sbjct: 145 RDRNPLVYGDDATIRAAMDAETRPVADRYFNVPNQSIDAMLESVDACAEAVTDRDLDVQY 204 Query: 206 GPAGVQWCSK 215 GP GVQWCS Sbjct: 205 GPTGVQWCSD 214 >ref|YP_771060.1| putative amidohydrolase [Rhizobium leguminosarum bv. viciae 3841] emb|CAK02969.1| putative amidohydrolase [Rhizobium leguminosarum bv. viciae 3841] Length = 462 Score = 92.4 bits (228), Expect = 6e-17, Method: Compositional matrix adjust. Identities = 68/181 (37%), Positives = 85/181 (46%), Gaps = 15/181 (8%) Query: 43 AKGPRSFILPAFVNAHDHARP-RASSFGAIGMPLESWILRTVVGTPVDAYLSAASAFARA 101 A GP ++P+ VNAHDH R FG + LE WI + DAYL A AFAR Sbjct: 34 ALGPGDLVMPSPVNAHDHGYGIRTLDFGVVDEALEVWIPGMRLRPRTDAYLEALVAFARL 93 Query: 102 AQAGCAAMMVHYTRPSGTMPLVEEARAIARAAADVGIRIAFAIAVRDQNPIVYGDSEAVL 161 AQ G A M H L +EA A+ RAA +VGIR+ + + D +P YG L Sbjct: 94 AQTGVGATM-HCHNSLNINRLGDEAAAVIRAAGEVGIRLGLSCPLLDFDPWAYGGGPEKL 152 Query: 162 SDLPAHDRKTIEQLFVRTPMSPQAYIELTDAIXAI-------GGPKVDVQLGPAGVQWCS 214 L + D +P P AY + + I A+ P VDVQ GP G QWCS Sbjct: 153 KPLLSEDDWQ-----ALSPSIP-AYAPVAEQIAAVDAVAAENSNPMVDVQYGPVGPQWCS 206 Query: 215 K 215 Sbjct: 207 N 207 >ref|ZP_08867720.1| cytosine deaminase [Azospirillum amazonense Y2] gb|EGY02689.1| cytosine deaminase [Azospirillum amazonense Y2] Length = 525 Score = 92.0 bits (227), Expect = 7e-17, Method: Compositional matrix adjust. Identities = 64/171 (37%), Positives = 90/171 (52%), Gaps = 9/171 (5%) Query: 47 RSFILPAFVNAHDHARPRAS-SFGAIGMPLESWILRTVVGTPVDAYLSAASAFARAAQAG 105 R ++PA NAHDH R +FGA PLE+W+ P+D Y AA AF+R A++G Sbjct: 89 RLLLMPALANAHDHGRGLGPLAFGAADQPLETWLPDLARQPPLDFYQCAAIAFSRMAESG 148 Query: 106 CAAM-MVHYTRPSGTMPLVEEARAIARAAADVGIRIAFAIAVRDQNPIVYGDSEAVLSDL 164 A+ H T+ S T L+ EA A++RAA DVGIR+AF D NP VYGD E + + + Sbjct: 149 IGAVNHCHNTQSSQT--LLAEAEAVSRAARDVGIRVAFCWPFFDANPFVYGDLEPLAAHM 206 Query: 165 PAHDRKTIE--QLFVRTPMSPQAYIELTDAIXAIGGPKVDVQLGPAGVQWC 213 A + + ++ + +R + A L D A +Q P G QW Sbjct: 207 DAAEFQALKGREASMRDCATNLA---LMDQARAFEHALFTLQYHPVGPQWV 254