BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0067.0024.p (199 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_001770423.1| amidohydrolase [Methylobacterium sp. 4-46] >... 177 8e-43 ref|ZP_08551077.1| cytosine deaminase [Salinisphaera shabanensis... 130 8e-29 ref|YP_001526809.1| cytosine deaminase [Azorhizobium caulinodans... 125 4e-27 emb|CAZ90212.1| putative hydrolase [Thiomonas sp. 3As] 122 3e-26 ref|YP_771060.1| putative amidohydrolase [Rhizobium leguminosaru... 110 1e-22 >ref|YP_001770423.1| amidohydrolase [Methylobacterium sp. 4-46] gb|ACA17989.1| amidohydrolase [Methylobacterium sp. 4-46] Length = 491 Score = 177 bits (449), Expect = 8e-43, Method: Compositional matrix adjust. Identities = 101/198 (51%), Positives = 129/198 (65%), Gaps = 1/198 (0%) Query: 1 MIAASGARIVTNFSSNLHLRSGHAPIAAAHSCGCGIAVGVDGLALDEDDDVLREMRLVQM 60 +IA +GA IVTNFSSNLHLRSG AP A + GC + +G+DGLA DEDDD+LRE+RLVQ Sbjct: 294 LIAEAGAMIVTNFSSNLHLRSGIAPFREARAQGCRVCLGLDGLAFDEDDDMLRELRLVQA 353 Query: 61 MHGGLGFKRTWTAQEFFALAIRNGRRATGAPGAGELAVGAPADFVGIDLDRLERDQILDA 120 +HG GF + F A I GR + GAPG G L GAPAD + +DLD L+RD IL Sbjct: 354 VHGPWGFADADDRRRFLAAVIAEGRASLGAPGPGLLEAGAPADLMVLDLDALDRDAILPV 413 Query: 121 DPLDLLFARGNASLISELVVDGRTVAKAGRCLGVDFPAIEQELRNIARASDATYAHVLRA 180 DPLDL+FAR A+ + ++VV GR V GR G+D PA E ELR R + +LRA Sbjct: 414 DPLDLVFARATAAHVRDVVVAGRRVVADGRVTGIDLPAAEAELRARYRLA-VPRDPLLRA 472 Query: 181 WPRLSNSVQHWFEAQLTC 198 WPR +++ W+ ++ C Sbjct: 473 WPRYERALRAWYARRIGC 490 >ref|ZP_08551077.1| cytosine deaminase [Salinisphaera shabanensis E1L3A] gb|EGM33595.1| cytosine deaminase [Salinisphaera shabanensis E1L3A] Length = 479 Score = 130 bits (328), Expect = 8e-29, Method: Compositional matrix adjust. Identities = 77/197 (39%), Positives = 107/197 (54%) Query: 2 IAASGARIVTNFSSNLHLRSGHAPIAAAHSCGCGIAVGVDGLALDEDDDVLREMRLVQMM 61 IA SGA I N SSNLHLRSG AP+ GC +A+G+DG ALD+DDD LREMRL +++ Sbjct: 283 IAESGATIAVNTSSNLHLRSGIAPLRRMLDTGCRVALGLDGSALDQDDDALREMRLTRLV 342 Query: 62 HGGLGFKRTWTAQEFFALAIRNGRRATGAPGAGELAVGAPADFVGIDLDRLERDQILDAD 121 H GF+ ++ A A +GR GAPG G LA G PAD++ +DL RL+R ++D Sbjct: 343 HADRGFESWAGHRDMLACATSHGRVCIGAPGDGRLAAGQPADWLVLDLARLQRYDLVDNP 402 Query: 122 PLDLLFARGNASLISELVVDGRTVAKAGRCLGVDFPAIEQELRNIARASDATYAHVLRAW 181 LF+ A+ I +L V G+ V + +D A+ EL + R + W Sbjct: 403 IPASLFSHTTAAHIDQLWVGGQPVVCDAALVNLDIAAVHDELIDQYRRRWTDRRPLQAVW 462 Query: 182 PRLSNSVQHWFEAQLTC 198 + V W++ C Sbjct: 463 SDIEAHVHGWYQQSGCC 479 >ref|YP_001526809.1| cytosine deaminase [Azorhizobium caulinodans ORS 571] dbj|BAF89891.1| putative cytosine deaminase [Azorhizobium caulinodans ORS 571] Length = 481 Score = 125 bits (313), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 80/195 (41%), Positives = 108/195 (55%), Gaps = 2/195 (1%) Query: 1 MIAASGARIVTNFSSNLHLRSGHAPIAAAHSCGCGIAVGVDGLALDEDDDVLREMRLVQM 60 ++A G IV+N SSNLHLRSG P+A + GC +A GVDG A+DEDDD+LRE+RL + Sbjct: 283 LLAERGVTIVSNPSSNLHLRSGIGPVAQMKARGCRVAFGVDGRAMDEDDDMLREIRLAHL 342 Query: 61 MHGGLGFKRTWTAQEFFALAIRNGRRAT-GAPGAGELAVGAPADFVGID-LDRLERDQIL 118 +H G GFK T +A+ NGRR+ G LA G PAD + +D + Sbjct: 343 LHLGTGFKAAVTQNAILEMALVNGRRSVLNRADGGRLAPGEPADLLVLDWARLEDDRLRD 402 Query: 119 DADPLDLLFARGNASLISELVVDGRTVAKAGRCLGVDFPAIEQELRNIARASDATYAHVL 178 D D ++L+ R A I EL+V GRT+ + G G+D A+ EL RA A A + Sbjct: 403 DLDAVELVVTRATARHIRELIVGGRTIVRDGAITGIDLRAVRAELLAQMRAGMAGMADLA 462 Query: 179 RAWPRLSNSVQHWFE 193 A P L ++ FE Sbjct: 463 AALPSLEAAMAAHFE 477 >emb|CAZ90212.1| putative hydrolase [Thiomonas sp. 3As] Length = 390 Score = 122 bits (306), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 78/186 (41%), Positives = 107/186 (57%), Gaps = 7/186 (3%) Query: 1 MIAASGARIVTNFSSNLHLRSGHAPIAAAHSCGCGIAVGVDGLALDEDDDVLREMRLVQM 60 ++A G I N SSNL L+SG AP+A GC +A+G+DG+ALDEDDD LREMRL Sbjct: 180 LLAERGVTIAVNTSSNLGLKSGVAPVAEMLRAGCRVAMGLDGMALDEDDDALREMRLAYA 239 Query: 61 MHGGLGFKRTWTAQEFFALAIRNGRRATGAPG------AGELAVGAPADFVGIDLDRLER 114 +H G G+ T T + +A A NG R P AG +A GAPAD + +D L+ Sbjct: 240 LHRGWGYDVTMTRAQLWAFAAANGDRVVRGPDAGRESLAGRIAPGAPADLLILDHADLDD 299 Query: 115 DQIL-DADPLDLLFARGNASLISELVVDGRTVAKAGRCLGVDFPAIEQELRNIARASDAT 173 D + D DPLD + AR I ++++DGRT+ G+ L +D PA+ EL AR+ + Sbjct: 300 DALFSDIDPLDYVLARAKGRHIEQVIIDGRTIVHRGKVLSLDEPALYAELLAQARSRLGS 359 Query: 174 YAHVLR 179 +A R Sbjct: 360 HAECGR 365 >ref|YP_771060.1| putative amidohydrolase [Rhizobium leguminosarum bv. viciae 3841] emb|CAK02969.1| putative amidohydrolase [Rhizobium leguminosarum bv. viciae 3841] Length = 462 Score = 110 bits (276), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 68/170 (40%), Positives = 95/170 (55%), Gaps = 9/170 (5%) Query: 1 MIAASGARIVTNFSSNLHLRSGHAPIAAAHSCGCGIAVGVDGLALDEDDDVLREMRLVQM 60 ++A G ++V+N S+NL LRSG API+ G +A+G+DG D+D D+ REMRL+ + Sbjct: 275 LLAERGVQLVSNPSANLRLRSGVAPISLVAEKGPALAIGLDGSGFDDDQDLWREMRLIHL 334 Query: 61 MHGGLGFKRTWTAQEFFALAIRNGRRATGAPGAGELAVGAPADFVGIDLDRLERDQIL-D 119 +HGG R +TA F AI G + AP AG+ DFV ID D L D + D Sbjct: 335 LHGGRELNRRFTADRIFDAAIHIGAKVLNAP-AGQ-------DFVVIDYDALTADSLFDD 386 Query: 120 ADPLDLLFARGNASLISELVVDGRTVAKAGRCLGVDFPAIEQELRNIARA 169 D ++L R ++ L + GR + + GR VDF A +ELR ARA Sbjct: 387 IDEAEVLLTRMTSAQAKGLFLGGREIMRDGRLTTVDFDAARRELREQARA 436