BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0067.0019.p (326 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_769421.1| 3-isopropylmalate dehydrogenase [Bradyrhizobium... 542 e-152 ref|YP_001238842.1| 3-isopropylmalate dehydrogenase [Bradyrhizob... 518 e-145 ref|YP_001204518.1| putative 3-isopropylmalate dehydrogenase (le... 513 e-143 ref|YP_002948108.1| 3-isopropylmalate dehydrogenase [Variovorax ... 353 3e-95 ref|ZP_02190268.1| homoserine O-acetyltransferase [alpha proteob... 343 2e-92 >ref|NP_769421.1| 3-isopropylmalate dehydrogenase [Bradyrhizobium japonicum USDA 110] dbj|BAC48046.1| 3-isopropylmalate dehydrogenase [Bradyrhizobium japonicum USDA 110] Length = 368 Score = 542 bits (1397), Expect = e-152, Method: Compositional matrix adjust. Identities = 272/360 (75%), Positives = 294/360 (81%), Gaps = 35/360 (9%) Query: 1 MSANDALHIAVLGGDGIGPEVMAPALDVLRRIEASAGLKFRFTEAPAGAGHYRETGKSMP 60 MSAN+A HIAVL GDGIGPEVMAPA++VLR+IE + L+FRFTEAPAGA +Y TGKSMP Sbjct: 10 MSANNAFHIAVLAGDGIGPEVMAPAIEVLRKIEQKSDLRFRFTEAPAGANNYLATGKSMP 69 Query: 61 ESTIRLCEEADAILLGACGLPSVRYPDNTEIAPQIELRMIFDLYAGVRPARLIPGVSSTI 120 E TI+LCEEADAILLGACGLPSVRYPDNTEIAPQIELR IFDLYAGVRPARLIPGV S I Sbjct: 70 ERTIKLCEEADAILLGACGLPSVRYPDNTEIAPQIELRFIFDLYAGVRPARLIPGVPSPI 129 Query: 121 VGADRKGIDLVVIRESTEGLFASMGK---------------------------------- 146 VGAD +GIDLVVIRESTEGLFASMGK Sbjct: 130 VGADTRGIDLVVIRESTEGLFASMGKGVVTHEDARETMVITRRTSERLFEFSFRLAARRK 189 Query: 147 XRGKVNGGLTCVDKANVFKAFAFFRNIFDETAKRHPDVRADHLYIDATAAALVKRPWDFD 206 RGK G LTCVDKANVFKAFAFFR IFDE AK+HP+VR D LY+DA +A LVKRPWDFD Sbjct: 190 ARGK-PGMLTCVDKANVFKAFAFFRGIFDEIAKKHPEVRTDRLYVDACSAMLVKRPWDFD 248 Query: 207 VMVTENMFGDILSDLTAGLIGGMGMAPSADIGDRYAVFQPCHGTAPDIMGQGKANPTAMI 266 VMV ENMFGDI+SD+TA LIGG+GMAPSADIGD+YAVFQPCHGTAPDIMGQGKANPT MI Sbjct: 249 VMVMENMFGDIVSDITASLIGGLGMAPSADIGDKYAVFQPCHGTAPDIMGQGKANPTGMI 308 Query: 267 LSAAMMLDWLADKHGLESAAEAGERIERAVDKVYADGIKPFEFGGSNGTTDVARAVLAAL 326 LSAAMMLDW+ADKHG+ESAAEAGE IERAVD+VYA GIKP EFGGSNGT D+ +AVL AL Sbjct: 309 LSAAMMLDWIADKHGVESAAEAGETIERAVDQVYAGGIKPMEFGGSNGTADITKAVLGAL 368 >ref|YP_001238842.1| 3-isopropylmalate dehydrogenase [Bradyrhizobium sp. BTAi1] gb|ABQ34936.1| 3-isopropylmalate dehydrogenase [Bradyrhizobium sp. BTAi1] Length = 359 Score = 518 bits (1334), Expect = e-145, Method: Compositional matrix adjust. Identities = 264/360 (73%), Positives = 289/360 (80%), Gaps = 35/360 (9%) Query: 1 MSANDALHIAVLGGDGIGPEVMAPALDVLRRIEASAGLKFRFTEAPAGAGHYRETGKSMP 60 MS+ ++LHIAVL GDGIGPEVMAPAL+VLR++EA AGL FRFTEA AGAG+Y TG S+ Sbjct: 1 MSSTNSLHIAVLPGDGIGPEVMAPALEVLRKVEAKAGLAFRFTEAQAGAGNYLATGVSIA 60 Query: 61 ESTIRLCEEADAILLGACGLPSVRYPDNTEIAPQIELRMIFDLYAGVRPARLIPGVSSTI 120 ESTIRLCE+ADAILLGACGLPSVRYPDNTEIAPQIELR FDLYAGVRPARLIPGV S I Sbjct: 61 ESTIRLCEQADAILLGACGLPSVRYPDNTEIAPQIELRFHFDLYAGVRPARLIPGVPSPI 120 Query: 121 VGADRKGIDLVVIRESTEGLFASMGK---------------------------------- 146 VGA +GID VVIRESTEGLFASMGK Sbjct: 121 VGAAERGIDFVVIRESTEGLFASMGKGVVTHEEARETLVITRKTSERLFNFSFKLAERRR 180 Query: 147 XRGKVNGGLTCVDKANVFKAFAFFRNIFDETAKRHPDVRADHLYIDATAAALVKRPWDFD 206 RG+ +G L CVDKANVFKAFA+FR IFDE A+ HP+V+A+ LYIDA A LVKRPWDFD Sbjct: 181 ARGR-SGILGCVDKANVFKAFAYFRKIFDEVAELHPNVKAERLYIDACALMLVKRPWDFD 239 Query: 207 VMVTENMFGDILSDLTAGLIGGMGMAPSADIGDRYAVFQPCHGTAPDIMGQGKANPTAMI 266 V+VTENMFGDILSDL AGLIGGMGMAPSADIGDR+AVFQPCHGTAPDIMGQGKANPTAMI Sbjct: 240 VLVTENMFGDILSDLAAGLIGGMGMAPSADIGDRHAVFQPCHGTAPDIMGQGKANPTAMI 299 Query: 267 LSAAMMLDWLADKHGLESAAEAGERIERAVDKVYADGIKPFEFGGSNGTTDVARAVLAAL 326 LSAAMMLDWLAD G+E AAEA +RIE+AVDKVYA G+KPFEFGGS GT +V +AVL AL Sbjct: 300 LSAAMMLDWLADTRGIEGAAEAAQRIEQAVDKVYASGLKPFEFGGSAGTLEVTKAVLEAL 359 >ref|YP_001204518.1| putative 3-isopropylmalate dehydrogenase (leuB) [Bradyrhizobium sp. ORS 278] emb|CAL76281.1| putative 3-isopropylmalate dehydrogenase (leuB) [Bradyrhizobium sp. ORS 278] Length = 359 Score = 513 bits (1321), Expect = e-143, Method: Compositional matrix adjust. Identities = 260/360 (72%), Positives = 285/360 (79%), Gaps = 35/360 (9%) Query: 1 MSANDALHIAVLGGDGIGPEVMAPALDVLRRIEASAGLKFRFTEAPAGAGHYRETGKSMP 60 M + +ALHIAVL GDGIGPEVMAPAL+VLR++EA AGL FRFTEA AGA +Y TG S+ Sbjct: 1 MPSTNALHIAVLPGDGIGPEVMAPALEVLRKVEARAGLSFRFTEAQAGANNYLATGVSIA 60 Query: 61 ESTIRLCEEADAILLGACGLPSVRYPDNTEIAPQIELRMIFDLYAGVRPARLIPGVSSTI 120 ESTIRLCE+ADAILLGACGLPSVRYPDNTEIAPQIELR FDLYAGVRPARLIPGV S I Sbjct: 61 ESTIRLCEQADAILLGACGLPSVRYPDNTEIAPQIELRFHFDLYAGVRPARLIPGVPSPI 120 Query: 121 VGADRKGIDLVVIRESTEGLFASMGK---------------------------------- 146 VGA +GID VVIRESTEGLFASMGK Sbjct: 121 VGAAERGIDFVVIRESTEGLFASMGKGQVTHEEARETLVITRRTSERLFNFSFKLAERRR 180 Query: 147 XRGKVNGGLTCVDKANVFKAFAFFRNIFDETAKRHPDVRADHLYIDATAAALVKRPWDFD 206 RG+ +G L CVDKANVFKAFA+FR IFDE A+ HP+V+ + LYIDA A LVKRPWDFD Sbjct: 181 ARGR-SGTLGCVDKANVFKAFAYFRKIFDEVAELHPEVKTERLYIDACALMLVKRPWDFD 239 Query: 207 VMVTENMFGDILSDLTAGLIGGMGMAPSADIGDRYAVFQPCHGTAPDIMGQGKANPTAMI 266 V+VTENMFGDILSDL AGL+GGMGMAPSADIGDR+AVFQPCHGTAPDIMGQGKANPTAMI Sbjct: 240 VLVTENMFGDILSDLAAGLVGGMGMAPSADIGDRHAVFQPCHGTAPDIMGQGKANPTAMI 299 Query: 267 LSAAMMLDWLADKHGLESAAEAGERIERAVDKVYADGIKPFEFGGSNGTTDVARAVLAAL 326 LSAAMMLDWLAD HG+E AAEA +RIE+AVD+VYA G+KPFEFGG GT DV +AVL L Sbjct: 300 LSAAMMLDWLADTHGIEGAAEAAQRIEQAVDRVYAAGLKPFEFGGHAGTIDVTKAVLQEL 359 >ref|YP_002948108.1| 3-isopropylmalate dehydrogenase [Variovorax paradoxus S110] gb|ACS22842.1| 3-isopropylmalate dehydrogenase [Variovorax paradoxus S110] Length = 363 Score = 353 bits (905), Expect = 3e-95, Method: Compositional matrix adjust. Identities = 190/356 (53%), Positives = 231/356 (64%), Gaps = 40/356 (11%) Query: 9 IAVLGGDGIGPEVMAPALDVLRRIEASAGLKFRFTEAPAGAGHYRETGKSMPESTIRLCE 68 IAVL GDGIG EVM A VL ++ G PAGA HY ++G ++PE+T+R CE Sbjct: 7 IAVLPGDGIGTEVMHAAQAVLEAVQPRVGRALALEWLPAGAQHYLDSGVALPETTLRRCE 66 Query: 69 EADAILLGACGLPSVRYPDNTEIAPQIELRMIFDLYAGVRPARLIPGVSSTIVGADRKGI 128 ADAIL GA GLP VR D TEI PQ++LRM FDLYAGVRP R G+ + + + I Sbjct: 67 SADAILFGAMGLPHVRGADGTEIIPQLDLRMHFDLYAGVRPIRTFRGLPTPLSNPKAEHI 126 Query: 129 DLVVIRESTEGLFASMGKXRGKVNGG---------------------------------- 154 DLV++RESTEGLF + G RG V G Sbjct: 127 DLVLVRESTEGLFYARG--RGDVRGDQEVFDTMRITRHGTTRICEFAFRLAARRAERLGR 184 Query: 155 ---LTCVDKANVFKAFAFFRNIFDETAKRHPDVRADHLYIDATAAALVKRPWDFDVMVTE 211 +T VDKANVF + AFFR +F+E A RHP V AD Y+DA A LV +PW +DV+VTE Sbjct: 185 AGRVTNVDKANVFASMAFFRKVFEEVAARHPAVAADSGYVDAMALNLVMKPWTYDVLVTE 244 Query: 212 NMFGDILSDLTAGLIGGMGMAPSADIGDRYAVFQPCHGTAPDIMGQGKANPTAMILSAAM 271 NMFGDILSDL A L+GGMGMAPS DIGDR+A+FQP HGTAPDI GQGKANPTAM+LSAAM Sbjct: 245 NMFGDILSDLIAALVGGMGMAPSGDIGDRHALFQPAHGTAPDIAGQGKANPTAMLLSAAM 304 Query: 272 MLDWLADKHGLESAAEAGERIERAVDKVYADG-IKPFEFGGSNGTTDVARAVLAAL 326 ML+WLA++ G + A+ IERAV++V+A G ++P EFGG +GT D+ RA AL Sbjct: 305 MLEWLAERSGDTALADGAALIERAVEQVFATGQVRPMEFGGMHGTADITRAATDAL 360 >ref|ZP_02190268.1| homoserine O-acetyltransferase [alpha proteobacterium BAL199] gb|EDP62991.1| homoserine O-acetyltransferase [alpha proteobacterium BAL199] Length = 367 Score = 343 bits (880), Expect = 2e-92, Method: Compositional matrix adjust. Identities = 182/349 (52%), Positives = 226/349 (64%), Gaps = 37/349 (10%) Query: 4 NDALHIAVLGGDGIGPEVMAPALDVLRRI-EASAGLKFRFTEAPAGAGHYRETGKSMPES 62 IAV GGDGIGPE+MAP + +L+ + S L F+F E AGA HYRETG+S+PE+ Sbjct: 2 RQTFKIAVFGGDGIGPEIMAPTVRLLQDLARESETLAFQFHEVAAGAAHYRETGESLPEA 61 Query: 63 TIRLCEEADAILLGACGLPSVRYPDNTEIAPQIELRMIFDLYAGVRPARLIPGVSSTIVG 122 ++ +ADAILL A GLP+VRYPD TEI+PQIELRM LYAGVRP + PG + Sbjct: 62 SLDTARKADAILLSAMGLPAVRYPDGTEISPQIELRMKLGLYAGVRPVFVQPGQDVPLKS 121 Query: 123 ADRKGIDLVVIRESTEGLFASMGK-----------------------------------X 147 + ID V+IRESTEGLFAS GK Sbjct: 122 SRVTDIDFVIIRESTEGLFASHGKGVVEDDKEARETLVITRAISEKLFDFAFRLARQRQQ 181 Query: 148 RGKVNGGLTCVDKANVFKAFAFFRNIFDETAKRHPDVRADHLYIDATAAALVKRPWDFDV 207 G+ G + CVDKANVF+AFAFFR IF E AK +P+++A+ Y+DATA +V++PW FDV Sbjct: 182 SGRGQGLVHCVDKANVFQAFAFFRKIFFERAKAYPEIKAETAYVDATALWMVQKPWMFDV 241 Query: 208 MVTENMFGDILSDLTAGLIGGMGMAPSADIGDRYAVFQPCHGTAPDIMGQGKANPTAMIL 267 +VTENMFGDILSDL AGL+G +G+APSADIGD +AVFQPCHG+APDI G+G ANP AMIL Sbjct: 242 LVTENMFGDILSDLGAGLMGSLGLAPSADIGDHHAVFQPCHGSAPDIAGKGIANPMAMIL 301 Query: 268 SAAMMLDWLADKHGLESAAEAGERIERAVDKVYADG-IKPFEFGGSNGT 315 SAAMML+WL +H A G+ + AV V +G + + GGS T Sbjct: 302 SAAMMLEWLGHEHRNTEAIAMGDALRTAVSDVLVEGRMLTADLGGSART 350