BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0066.0094.p (254 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_001117827.1| enoyl-CoA hydratase/isomerase [Burkholderia ... 266 2e-69 ref|YP_624760.1| enoyl-CoA hydratase/isomerase [Burkholderia cen... 265 4e-69 ref|YP_778386.1| enoyl-CoA hydratase/isomerase [Burkholderia amb... 263 3e-68 ref|ZP_01667133.1| Enoyl-CoA hydratase/isomerase [Thermosinus ca... 132 6e-29 ref|YP_003427655.1| enoyl-CoA hydratase [Bacillus pseudofirmus O... 129 3e-28 >ref|YP_001117827.1| enoyl-CoA hydratase/isomerase [Burkholderia vietnamiensis G4] gb|ABO58362.1| Enoyl-CoA hydratase/isomerase [Burkholderia vietnamiensis G4] Length = 262 Score = 266 bits (680), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 142/253 (56%), Positives = 182/253 (71%) Query: 1 MASSDILFIERDADIVVMTMNRPPANALNIDLISVLLDRIQTFTRDEAPPGIVLTGCGDR 60 M + +++ DAD+V +T++RPPANALN LI+ L+ + F R + PPGIVLTG G++ Sbjct: 9 MKTDELICTRIDADVVQLTIDRPPANALNEPLITALMSVFRDFARHDVPPGIVLTGAGEK 68 Query: 61 FFSAGGDIKEVATVEAATPRLRMFHALLCEMERYPSPLVCAVRGYAVGGAFEFLLHADYV 120 FF AGGDI EVA E A R+ FHA L ++E+YPSPLVCAV GYAVG FE LHADYV Sbjct: 69 FFCAGGDINEVADHELALSRMEAFHAFLAQLEQYPSPLVCAVNGYAVGAGFEMALHADYV 128 Query: 121 VANPECQVGFPEINHGLLPAVKGMRQAARILGQRAAKELLYWGELIDAPKALRLGAVDQI 180 VA+ E ++GFPEINHGLLPA KGMR AA +LG+RAA+ LL+ GELI A +AL +GAVD I Sbjct: 129 VASAESRLGFPEINHGLLPAAKGMRHAAALLGRRAARSLLFSGELITAGEALAIGAVDVI 188 Query: 181 VETDEVTDKARSICRQLRSKDRKLFAAMKRSLNLTGQMSDGELDDMTIADLPAYLDEEES 240 DE A + CR L +KDR+LFAA+KR+L + +M+D L MT+ DL YL + Sbjct: 189 SAHDETLRDALAKCRALCAKDRQLFAAIKRTLRESPRMNDEALLQMTLDDLRGYLGNART 248 Query: 241 AEARARFLAKNKK 253 A+AR+RFL +NKK Sbjct: 249 ADARSRFLNRNKK 261 >ref|YP_624760.1| enoyl-CoA hydratase/isomerase [Burkholderia cenocepacia AU 1054] ref|YP_836890.1| enoyl-CoA hydratase/isomerase [Burkholderia cenocepacia HI2424] gb|ABF79787.1| Enoyl-CoA hydratase/isomerase [Burkholderia cenocepacia AU 1054] gb|ABK09997.1| Enoyl-CoA hydratase/isomerase [Burkholderia cenocepacia HI2424] Length = 254 Score = 265 bits (677), Expect = 4e-69, Method: Compositional matrix adjust. Identities = 141/253 (55%), Positives = 181/253 (71%) Query: 1 MASSDILFIERDADIVVMTMNRPPANALNIDLISVLLDRIQTFTRDEAPPGIVLTGCGDR 60 M + +++ DAD+V +T++RPPANALN LI+ L+ + F R + PPGIV TG G++ Sbjct: 1 MKTDELICTRIDADVVQLTIDRPPANALNEPLITALMSVFRDFARHDVPPGIVFTGAGEK 60 Query: 61 FFSAGGDIKEVATVEAATPRLRMFHALLCEMERYPSPLVCAVRGYAVGGAFEFLLHADYV 120 FF AGGDI EVA E A R+ FHA L ++E+YPSPLVCAV GYAVG FE LHADYV Sbjct: 61 FFCAGGDINEVADHELALSRMEAFHAFLAQLEQYPSPLVCAVNGYAVGAGFEMALHADYV 120 Query: 121 VANPECQVGFPEINHGLLPAVKGMRQAARILGQRAAKELLYWGELIDAPKALRLGAVDQI 180 VA+ E ++GFPEINHGLLPA KGMR AA +LG+RAA+ LL+ GELI A +AL +GAVD I Sbjct: 121 VASAESRLGFPEINHGLLPAAKGMRHAAALLGRRAARSLLFSGELITAGEALAIGAVDAI 180 Query: 181 VETDEVTDKARSICRQLRSKDRKLFAAMKRSLNLTGQMSDGELDDMTIADLPAYLDEEES 240 DE A + CR L +KDR+LFAA+KR+L + +M+D L MT+ DL YL + Sbjct: 181 SAHDETLRDALAKCRALCAKDRQLFAAIKRTLRESPRMNDEALLQMTLDDLRGYLGNART 240 Query: 241 AEARARFLAKNKK 253 A+AR+RFL +NKK Sbjct: 241 ADARSRFLNRNKK 253 >ref|YP_778386.1| enoyl-CoA hydratase/isomerase [Burkholderia ambifaria AMMD] gb|ABI92052.1| Enoyl-CoA hydratase/isomerase [Burkholderia ambifaria AMMD] Length = 262 Score = 263 bits (671), Expect = 3e-68, Method: Compositional matrix adjust. Identities = 139/254 (54%), Positives = 183/254 (72%) Query: 1 MASSDILFIERDADIVVMTMNRPPANALNIDLISVLLDRIQTFTRDEAPPGIVLTGCGDR 60 M + +++ D D+V +T+NRPPANAL+ LI+ L+ + + + PPGIVLTG G++ Sbjct: 9 MKTDELIRTHIDTDVVRLTINRPPANALSEPLITALMSIFRDLAQQDVPPGIVLTGAGEK 68 Query: 61 FFSAGGDIKEVATVEAATPRLRMFHALLCEMERYPSPLVCAVRGYAVGGAFEFLLHADYV 120 FF AGGDI EVA E A R+ FHA L ++E+YPSPLVCAV GYAVG FE +LHADYV Sbjct: 69 FFCAGGDINEVADHELALSRMEAFHAFLAQLEQYPSPLVCAVNGYAVGAGFEMVLHADYV 128 Query: 121 VANPECQVGFPEINHGLLPAVKGMRQAARILGQRAAKELLYWGELIDAPKALRLGAVDQI 180 VA+ E ++GFPEINHGLLPA KGMR AA +LG+RAA+ LL+ GELI A +AL +GAVD I Sbjct: 129 VASAEARLGFPEINHGLLPAAKGMRHAAALLGRRAARSLLFSGELITAGEALAIGAVDAI 188 Query: 181 VETDEVTDKARSICRQLRSKDRKLFAAMKRSLNLTGQMSDGELDDMTIADLPAYLDEEES 240 DE A + CR+L +KD +LFAA+KR+L + +M+D L MT+ DL YL ++ Sbjct: 189 SARDETLRDALAKCRELCTKDPQLFAAIKRTLRESPRMNDEALLQMTLDDLRGYLGNAQT 248 Query: 241 AEARARFLAKNKKV 254 A+AR+RFL +NKKV Sbjct: 249 ADARSRFLNRNKKV 262 >ref|ZP_01667133.1| Enoyl-CoA hydratase/isomerase [Thermosinus carboxydivorans Nor1] gb|EAX47035.1| Enoyl-CoA hydratase/isomerase [Thermosinus carboxydivorans Nor1] Length = 259 Score = 132 bits (331), Expect = 6e-29, Method: Compositional matrix adjust. Identities = 85/261 (32%), Positives = 146/261 (55%), Gaps = 19/261 (7%) Query: 5 DILFIERDADIVVMTMNRPP-ANALNIDLISVLLDRI-QTFTRDEAPPGIVLTGCGDRFF 62 D + +ERD I V+ +NRP NALN+ + + LDRI ++ D++ +++TG G++ F Sbjct: 3 DNIVVERDGAIAVIIVNRPKEMNALNLATM-LELDRIIRSLEDDQSVGALIITGSGEKAF 61 Query: 63 SAGGDIKEVATVEA--ATPRLRMFHALLCEMERYPSPLVCAVRGYAVGGAFEFLLHADYV 120 AG DI E+A + A A R+ + +E +P P++ AV GYA+GG E + D Sbjct: 62 VAGADISEMADMGAMQAKEWARLGQQVFSRIENFPRPVIAAVNGYALGGGCELAMACDIR 121 Query: 121 VANPECQVGFPEINHGLLPAVKGMRQAARILGQRAAKELLYWGELIDAPKALRLGAVDQI 180 +A+ + + G PE+N G++P G ++ R++G+ AK L++ G++IDA +ALR+G VD++ Sbjct: 122 LASEKAKFGQPEVNLGIIPGFGGTQRLTRLVGKGQAKLLIFSGDIIDAQEALRIGLVDRV 181 Query: 181 VETDEVTDKARSICRQLRSKDRKLFAAMKRSLNL-------TGQMSDGELDDMTIADLPA 233 V DE+ A+++ ++ +K + K ++N +G M + E+ M Sbjct: 182 VSADELMVAAKALANKMIAKAPLAVSQAKLAINKGVEMDSESGYMFESEIFGMCF----T 237 Query: 234 YLDEEESAEARARFLAKNKKV 254 D++E A FL K K V Sbjct: 238 TADQKEGMRA---FLEKRKPV 255 >ref|YP_003427655.1| enoyl-CoA hydratase [Bacillus pseudofirmus OF4] gb|ADC50763.1| enoyl-CoA hydratase [Bacillus pseudofirmus OF4] Length = 259 Score = 129 bits (325), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 69/215 (32%), Positives = 117/215 (54%), Gaps = 5/215 (2%) Query: 9 IERDADIVVMTMNRPPANALNIDLISVLLDRIQTFTRDEAPPGIVLTGCGDRFFSAGGDI 68 +E++ + ++T+N PP N ++ D+I+ L D Q ++D ++LTG GD+ F AG DI Sbjct: 7 VEKEKGLAIVTINNPPLNVMDKDVIASLRDTFQQLSQDPETVVVILTGAGDKAFMAGADI 66 Query: 69 KEVATVEAATPRLRM----FHALLCEMERYPSPLVCAVRGYAVGGAFEFLLHADYVVANP 124 KE + P ++ HA+L +++ + P + + G GG E L D +A Sbjct: 67 KEFPQL-MDHPNMKEPVMETHAVLNQIDFFEKPTIAVLNGLTFGGGCELALTFDIRIAEE 125 Query: 125 ECQVGFPEINHGLLPAVKGMRQAARILGQRAAKELLYWGELIDAPKALRLGAVDQIVETD 184 Q+G PEI GL P G ++ R++G+ AKEL+Y GE IDA A ++G V+ + ++ Sbjct: 126 HTQIGLPEIKLGLFPGGGGTQRLPRLIGEAKAKELMYTGEPIDAKTAQQIGLVNHVTKSG 185 Query: 185 EVTDKARSICRQLRSKDRKLFAAMKRSLNLTGQMS 219 +KA I +Q+R + +KR+++ MS Sbjct: 186 AGLEKAVQIAKQIRRHSLPALSCIKRAVDEGMDMS 220