BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0066.0022.p (453 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_769600.1| amidase [Bradyrhizobium japonicum USDA 110] >gi... 702 0.0 ref|YP_001204643.1| amidase [Bradyrhizobium sp. ORS 278] >gi|146... 690 0.0 ref|YP_569017.1| amidase [Rhodopseudomonas palustris BisB5] >gi|... 688 0.0 ref|YP_001238967.1| amidase [Bradyrhizobium sp. BTAi1] >gi|14640... 686 0.0 ref|NP_947123.1| amidase [Rhodopseudomonas palustris CGA009] >gi... 663 0.0 >ref|NP_769600.1| amidase [Bradyrhizobium japonicum USDA 110] dbj|BAC48225.1| glutamyl-tRNA(Gln) amidotransferase subunit [Bradyrhizobium japonicum USDA 110] Length = 449 Score = 702 bits (1811), Expect = 0.0, Method: Compositional matrix adjust. Identities = 356/453 (78%), Positives = 394/453 (86%), Gaps = 4/453 (0%) Query: 1 MPKNPTLASLAADLASGATSARKLVEQCIARIADPSGEGQRTFIHVDRDAALEAADAMDR 60 MP PTLA L+ DL SG T++RKLVE C+ARIADP+GEGQRTFIHVD+DAAL AADAMD Sbjct: 1 MPDFPTLAKLSDDLESGRTTSRKLVEACLARIADPAGEGQRTFIHVDKDAALAAADAMDA 60 Query: 61 LRKANAAPSPFAGIPVSIKDLFDIKGQVTRAGSRALEDSAPAEADAPVVARLKRAGFVVI 120 LRK AAPS +AGIP+SIKDLFDIKGQVTRAGSRAL+DS AE DA V RL++AGFV+I Sbjct: 61 LRKVKAAPSRYAGIPISIKDLFDIKGQVTRAGSRALDDSPAAEHDAATVERLRKAGFVLI 120 Query: 121 GRTNMTEFAYSGIGINPHYGTPKSAWNRSVGHVPGGSSSGAAVSIADGMAFGALGTDTGG 180 GRTNMTEFAYSGIGINPHYGTPK AWNR+ GHVPGGSSSGAAVS+ DGMA GALGTDTGG Sbjct: 121 GRTNMTEFAYSGIGINPHYGTPKGAWNRAEGHVPGGSSSGAAVSVLDGMAHGALGTDTGG 180 Query: 181 SCRIPAAFNGIVGYKPTQRRIPLDGGVPLSFTLDSYGPLANSVACCAALDAVLADQPVTS 240 SCRIPAAFNGIVGYKPTQRR+PLDG VPLSF+LDS GPLA SV+CCA LDAVLA++PV + Sbjct: 181 SCRIPAAFNGIVGYKPTQRRVPLDGAVPLSFSLDSIGPLARSVSCCAILDAVLANEPVVA 240 Query: 241 LTPRPVKGMRLAVPTTVPTTVALDDLDDAVAKTFERALASLSRAGALIERIEVPELLDVG 300 L PRPVKGMRLA VPTT+ALDDLD AVA+TFERAL +L+ GA+IERIE+ E D+G Sbjct: 241 LRPRPVKGMRLA----VPTTIALDDLDKAVAETFERALKTLADHGAIIERIEMAEFHDIG 296 Query: 301 VMNAKGGFAAAESYAWHRFLLASKGDIYDPRVAVRIQRGEGFPVADYIDLLNARRSFIAR 360 MNAKGGFAA+ESYAWHR+L+ +KGD+YDPRV+VRI RGE ADYIDLLN RRS IAR Sbjct: 297 PMNAKGGFAASESYAWHRYLITTKGDVYDPRVSVRIMRGEAQSAADYIDLLNERRSLIAR 356 Query: 361 TETRIAPYDAMVLPTTANLPPTIASLADDKAFATENLRALRNCTLINVLDGCAISLPAHR 420 IAPYDA+VLPTTAN PP IA LADDKAF T+NLRALRNCTLIN++DGCAISLPAHR Sbjct: 357 VNAHIAPYDALVLPTTANTPPKIADLADDKAFTTQNLRALRNCTLINMIDGCAISLPAHR 416 Query: 421 EGEAPVGLMLAAAGGSDRRIFELAAGMETVIRV 453 EG+ PVGLMLA AGGSDRRIFELAAGME VIRV Sbjct: 417 EGDVPVGLMLAGAGGSDRRIFELAAGMEAVIRV 449 >ref|YP_001204643.1| amidase [Bradyrhizobium sp. ORS 278] emb|CAL76406.1| Putative Glu-tRNA amidotransferase [Bradyrhizobium sp. ORS 278] Length = 450 Score = 690 bits (1780), Expect = 0.0, Method: Compositional matrix adjust. Identities = 350/449 (77%), Positives = 393/449 (87%), Gaps = 4/449 (0%) Query: 4 NPTLASLAADLASGATSARKLVEQCIARIADPSGEGQRTFIHVDRDAALEAADAMDRLRK 63 NPTLA+LA DLA+G T++RKLVE C+ RIADP+GEGQR FIHVD+DAAL AAD MD LRK Sbjct: 5 NPTLAALADDLAAGRTTSRKLVEDCLVRIADPAGEGQRAFIHVDKDAALSAADGMDALRK 64 Query: 64 ANAAPSPFAGIPVSIKDLFDIKGQVTRAGSRALEDSAPAEADAPVVARLKRAGFVVIGRT 123 ANAAPS FAGIPVSIKDLFDIKGQVTRAGSRALEDSAPAEADAP VARL++AGFVVIGRT Sbjct: 65 ANAAPSAFAGIPVSIKDLFDIKGQVTRAGSRALEDSAPAEADAPAVARLRKAGFVVIGRT 124 Query: 124 NMTEFAYSGIGINPHYGTPKSAWNRSVGHVPGGSSSGAAVSIADGMAFGALGTDTGGSCR 183 NMTEFAYSGIGINPHYGTPKSAW R VG+VPGGSSSGAAVS+AD MA+GALGTDTGGSCR Sbjct: 125 NMTEFAYSGIGINPHYGTPKSAWKREVGYVPGGSSSGAAVSVADRMAYGALGTDTGGSCR 184 Query: 184 IPAAFNGIVGYKPTQRRIPLDGGVPLSFTLDSYGPLANSVACCAALDAVLADQPVTSLTP 243 IPAAFNGI GYKPTQ R+PLDGGVPLS TLDS+GPLAN+VACCA LD+VLAD+P+ L Sbjct: 185 IPAAFNGITGYKPTQARVPLDGGVPLSSTLDSFGPLANTVACCAVLDSVLADEPIRPLAS 244 Query: 244 RPVKGMRLAVPTTVPTTVALDDLDDAVAKTFERALASLSRAGALIERIEVPELLDVGVMN 303 RPVKG+RLA VPTT+ LD+LD VA+TFERAL +L+R GALIERIE PE LDVGV+ Sbjct: 245 RPVKGLRLA----VPTTIVLDELDAEVAETFERALDTLARQGALIERIEFPEFLDVGVIG 300 Query: 304 AKGGFAAAESYAWHRFLLASKGDIYDPRVAVRIQRGEGFPVADYIDLLNARRSFIARTET 363 KGGFAAAESYAWHRFLL +KGD+YDPRV+VRI RGE V DYI++LNARRS + R Sbjct: 301 MKGGFAAAESYAWHRFLLTAKGDVYDPRVSVRILRGEAITVPDYIEMLNARRSLVTRAAA 360 Query: 364 RIAPYDAMVLPTTANLPPTIASLADDKAFATENLRALRNCTLINVLDGCAISLPAHREGE 423 RIAPYDA+V+PTTAN PP IA LADD AFA EN+RALRNCT IN++DGCAISLPAHR GE Sbjct: 361 RIAPYDALVMPTTANAPPKIADLADDAAFARENIRALRNCTFINMIDGCAISLPAHRHGE 420 Query: 424 APVGLMLAAAGGSDRRIFELAAGMETVIR 452 PVGLMLA +GG+DR++ E+AAG+E V+R Sbjct: 421 VPVGLMLAQSGGNDRKLLEIAAGIEGVVR 449 >ref|YP_569017.1| amidase [Rhodopseudomonas palustris BisB5] gb|ABE39116.1| Amidase [Rhodopseudomonas palustris BisB5] Length = 463 Score = 688 bits (1775), Expect = 0.0, Method: Compositional matrix adjust. Identities = 337/452 (74%), Positives = 385/452 (85%), Gaps = 4/452 (0%) Query: 1 MPKNPTLASLAADLASGATSARKLVEQCIARIADPSGEGQRTFIHVDRDAALEAADAMDR 60 M K+PTLASLAADL +G T+ARKLV+ C+ARIAD +GEG RTFIHVDRDAA++AA+AMD+ Sbjct: 15 MTKHPTLASLAADLDAGRTTARKLVDDCLARIADQAGEGARTFIHVDRDAAIKAAEAMDK 74 Query: 61 LRKANAAPSPFAGIPVSIKDLFDIKGQVTRAGSRALEDSAPAEADAPVVARLKRAGFVVI 120 LR+ AAPSPFAGIP+SIKDLFDIKGQVTRAGSRAL+D+ PAEADAP VARL+RAGFVVI Sbjct: 75 LREIKAAPSPFAGIPISIKDLFDIKGQVTRAGSRALDDNPPAEADAPAVARLRRAGFVVI 134 Query: 121 GRTNMTEFAYSGIGINPHYGTPKSAWNRSVGHVPGGSSSGAAVSIADGMAFGALGTDTGG 180 GRTNMTEFAYSGIGINPHYGTPK+A+NR GHVPGGSSSGAAVS+ADGMA LGTDTGG Sbjct: 135 GRTNMTEFAYSGIGINPHYGTPKAAFNRDAGHVPGGSSSGAAVSVADGMAHAGLGTDTGG 194 Query: 181 SCRIPAAFNGIVGYKPTQRRIPLDGGVPLSFTLDSYGPLANSVACCAALDAVLADQPVTS 240 SCRIPAAFNGIVGYKP+Q R+P DG VPLSF+LDS GPLA SV CCA LDAVLAD P+ Sbjct: 195 SCRIPAAFNGIVGYKPSQHRVPRDGAVPLSFSLDSIGPLARSVDCCAVLDAVLADAPIVP 254 Query: 241 LTPRPVKGMRLAVPTTVPTTVALDDLDDAVAKTFERALASLSRAGALIERIEVPELLDVG 300 L PR +KG+RLA VPTTVALD+LDDAV+ FERAL +LS GA+IE+IE+PE LDV Sbjct: 255 LAPRSLKGLRLA----VPTTVALDELDDAVSVAFERALKTLSGHGAIIEKIELPEFLDVA 310 Query: 301 VMNAKGGFAAAESYAWHRFLLASKGDIYDPRVAVRIQRGEGFPVADYIDLLNARRSFIAR 360 M AKGGFAAAESYAWHR+L+ ++GD+YDPRV RI RGE ADY+DL+ AR+S IAR Sbjct: 311 PMMAKGGFAAAESYAWHRYLIVAQGDVYDPRVRERILRGETQSAADYVDLITARKSLIAR 370 Query: 361 TETRIAPYDAMVLPTTANLPPTIASLADDKAFATENLRALRNCTLINVLDGCAISLPAHR 420 R+APYDA+V+PTTAN PP IA LADDKAF NL ALRNCTLIN++DGCAISLP HR Sbjct: 371 ACARLAPYDALVMPTTANTPPKIADLADDKAFTKANLLALRNCTLINMIDGCAISLPCHR 430 Query: 421 EGEAPVGLMLAAAGGSDRRIFELAAGMETVIR 452 +GEAPVGLMLA G+DR IFE+AA +E V+R Sbjct: 431 DGEAPVGLMLAGVNGADREIFEIAASLEMVVR 462 >ref|YP_001238967.1| amidase [Bradyrhizobium sp. BTAi1] gb|ABQ35061.1| Putative Glu-tRNA amidotransferase [Bradyrhizobium sp. BTAi1] Length = 450 Score = 686 bits (1769), Expect = 0.0, Method: Compositional matrix adjust. Identities = 348/449 (77%), Positives = 397/449 (88%), Gaps = 4/449 (0%) Query: 4 NPTLASLAADLASGATSARKLVEQCIARIADPSGEGQRTFIHVDRDAALEAADAMDRLRK 63 NPTLA+LAADLA+G ++R+LVE C+ARIADP+GEGQR FIHVD+ AAL AAD MD LRK Sbjct: 5 NPTLAALAADLAAGRMTSRQLVEACLARIADPAGEGQRAFIHVDKAAALAAADGMDALRK 64 Query: 64 ANAAPSPFAGIPVSIKDLFDIKGQVTRAGSRALEDSAPAEADAPVVARLKRAGFVVIGRT 123 ANAAPSP+AGIPVSIKDLFDIKGQVTRAGSRALEDSAPA+ADAPVVARL+RAGFVVIGRT Sbjct: 65 ANAAPSPYAGIPVSIKDLFDIKGQVTRAGSRALEDSAPADADAPVVARLRRAGFVVIGRT 124 Query: 124 NMTEFAYSGIGINPHYGTPKSAWNRSVGHVPGGSSSGAAVSIADGMAFGALGTDTGGSCR 183 NMTEFAYSGIGINPHYGTPKSAW R +GHVPGGSSSGAAVS+ D MA+GALGTDTGGSCR Sbjct: 125 NMTEFAYSGIGINPHYGTPKSAWKREIGHVPGGSSSGAAVSVVDRMAYGALGTDTGGSCR 184 Query: 184 IPAAFNGIVGYKPTQRRIPLDGGVPLSFTLDSYGPLANSVACCAALDAVLADQPVTSLTP 243 IPAAFNGI G+KPTQ R+PLDGGVPLS TLDS+GPLAN+VACCA LD+VLAD+P+ L P Sbjct: 185 IPAAFNGITGFKPTQARVPLDGGVPLSTTLDSFGPLANTVACCAVLDSVLADEPIRPLAP 244 Query: 244 RPVKGMRLAVPTTVPTTVALDDLDDAVAKTFERALASLSRAGALIERIEVPELLDVGVMN 303 RPVKG+RLA VPTTV LD+LD VA+TFERAL +L++ GALIERIE PE LDVG++ Sbjct: 245 RPVKGLRLA----VPTTVVLDELDVEVAETFERALETLAKQGALIERIEFPEFLDVGLIG 300 Query: 304 AKGGFAAAESYAWHRFLLASKGDIYDPRVAVRIQRGEGFPVADYIDLLNARRSFIARTET 363 KGGFAAAESYAWHRFLL +KGD+YDPRV+VRI RGE V DYI++LNARRS +AR Sbjct: 301 MKGGFAAAESYAWHRFLLTAKGDVYDPRVSVRIMRGEAITVPDYIEMLNARRSLVARAAA 360 Query: 364 RIAPYDAMVLPTTANLPPTIASLADDKAFATENLRALRNCTLINVLDGCAISLPAHREGE 423 RIAPYDA+V+PTTAN PP IA LADD+AFA EN+RALRNCT IN++DGCAISLPAHR G+ Sbjct: 361 RIAPYDALVMPTTANAPPKIADLADDQAFARENIRALRNCTFINMIDGCAISLPAHRHGD 420 Query: 424 APVGLMLAAAGGSDRRIFELAAGMETVIR 452 PVGLMLA +GG+DR++ E+AAG+E V+R Sbjct: 421 VPVGLMLAQSGGNDRKLLEIAAGVEGVVR 449 >ref|NP_947123.1| amidase [Rhodopseudomonas palustris CGA009] emb|CAE27219.1| putative Glu-tRNA amidotransferase [Rhodopseudomonas palustris CGA009] Length = 452 Score = 663 bits (1710), Expect = 0.0, Method: Compositional matrix adjust. Identities = 331/452 (73%), Positives = 382/452 (84%), Gaps = 4/452 (0%) Query: 1 MPKNPTLASLAADLASGATSARKLVEQCIARIADPSGEGQRTFIHVDRDAALEAADAMDR 60 M ++PTLA+LAADL +G T+ARKLVE C+ARIADP+GEG R FIHVDRDAAL+AADAMD+ Sbjct: 4 MTRHPTLAALAADLEAGRTTARKLVEDCLARIADPAGEGARAFIHVDRDAALQAADAMDK 63 Query: 61 LRKANAAPSPFAGIPVSIKDLFDIKGQVTRAGSRALEDSAPAEADAPVVARLKRAGFVVI 120 LR A AAPSPFAGIP+SIKDLFDIKGQVTRAGSRAL+D+ PA++DAP VARL+RAGFVVI Sbjct: 64 LRAAKAAPSPFAGIPISIKDLFDIKGQVTRAGSRALDDNPPADSDAPAVARLRRAGFVVI 123 Query: 121 GRTNMTEFAYSGIGINPHYGTPKSAWNRSVGHVPGGSSSGAAVSIADGMAFGALGTDTGG 180 GR+NMTEFAYSGIGINPHYGTPK+A+ R+VG+VPGGSSSGAAVS+ADGMA LGTDTGG Sbjct: 124 GRSNMTEFAYSGIGINPHYGTPKAAYRRNVGYVPGGSSSGAAVSVADGMAHAGLGTDTGG 183 Query: 181 SCRIPAAFNGIVGYKPTQRRIPLDGGVPLSFTLDSYGPLANSVACCAALDAVLADQPVTS 240 SCRIPAAFNGIVGYKP+Q R+P DG VPLSF+LDS GPLA SV CCA LDA+LADQPV Sbjct: 184 SCRIPAAFNGIVGYKPSQYRVPRDGAVPLSFSLDSIGPLARSVECCATLDAILADQPVGP 243 Query: 241 LTPRPVKGMRLAVPTTVPTTVALDDLDDAVAKTFERALASLSRAGALIERIEVPELLDVG 300 L RP+KGMR+A VPTTVALDDLD AV FERAL +L+ G +IE+IEVPE +VG Sbjct: 244 LIARPLKGMRIA----VPTTVALDDLDAAVTAAFERALKTLASHGVIIEKIEVPEFAEVG 299 Query: 301 VMNAKGGFAAAESYAWHRFLLASKGDIYDPRVAVRIQRGEGFPVADYIDLLNARRSFIAR 360 + AKGGFAAAESYAWHR+L+ ++GD+YDPRV RI RGE ADY+DL+NAR+S IAR Sbjct: 300 PLGAKGGFAAAESYAWHRYLIVAQGDVYDPRVRERILRGETQSAADYVDLVNARKSLIAR 359 Query: 361 TETRIAPYDAMVLPTTANLPPTIASLADDKAFATENLRALRNCTLINVLDGCAISLPAHR 420 R+APYDA+ +PTTA PTIA LADDKAF NL LRNCTLIN++DGCAISLP H+ Sbjct: 360 ASARLAPYDAVAMPTTAIAAPTIAELADDKAFTKANLLTLRNCTLINMIDGCAISLPCHQ 419 Query: 421 EGEAPVGLMLAAAGGSDRRIFELAAGMETVIR 452 +GE P+GLMLA G+DR IFE+AAGME VIR Sbjct: 420 DGEPPIGLMLAGVNGTDREIFEVAAGMEAVIR 451