BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0061.0021.p (404 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_771590.1| L-asparaginase [Bradyrhizobium japonicum USDA 1... 439 e-121 ref|ZP_01764925.1| L-asparaginase II [Burkholderia pseudomallei ... 412 e-113 ref|YP_002909387.1| putative L-asparaginase [Burkholderia glumae... 412 e-113 ref|YP_112062.1| L-asparaginase [Burkholderia pseudomallei K9624... 412 e-113 ref|ZP_02487028.1| L-asparaginase [Burkholderia pseudomallei 789... 412 e-113 >ref|NP_771590.1| L-asparaginase [Bradyrhizobium japonicum USDA 110] dbj|BAC50215.1| bll4950 [Bradyrhizobium japonicum USDA 110] Length = 379 Score = 439 bits (1130), Expect = e-121, Method: Compositional matrix adjust. Identities = 214/332 (64%), Positives = 267/332 (80%), Gaps = 1/332 (0%) Query: 72 QQATGTTLARVLVLGTGGTIAGQASARAGNAYDSGKVSAANLIAAIPGIDKLSQISAEQI 131 + A+ L R+LVL TGGTIAGQA ARA AY SG+++ L+ ++PG+DKL++++AEQI Sbjct: 44 KDASAGGLPRILVLATGGTIAGQADARATGAYKSGQITGEQLMQSVPGLDKLAKLNAEQI 103 Query: 132 SSIGSQDMNDNVWFDLVKRIRAAVDNKEVDGVVITHGTDTMEETAFFLQNVLDTDVPIVL 191 SSIGSQDMND VWF L +RI+ A D E DGV++THGTDT+EETAFFL NV+ D P+V+ Sbjct: 104 SSIGSQDMNDKVWFALARRIQEAFDKNEADGVIVTHGTDTLEETAFFLDNVVRGDKPVVI 163 Query: 192 VGSMRPSTAIGADGPANLYEAMRVASAPESRGRGVLVVLNDTIHAARWVQKTNTTSVETF 251 VGSMRP++A+ ADGP NLYEA++VA+ P SRGRGV+ VLND I AR V KTNTTS+ETF Sbjct: 164 VGSMRPASAVSADGPGNLYEAVQVAADPRSRGRGVMAVLNDKIEGARSVTKTNTTSIETF 223 Query: 252 RSPDAGPVGYVDT-GSLRFLQPAAPARGAKLKLPDAPPLPRVDIIYSHANMDAAEVEDAV 310 SP+ GP+GYVDT G +RF+ AA + A +LP LPRV+I+YSHANMDA +EDA+ Sbjct: 224 NSPNGGPIGYVDTAGGIRFMAQAAGLKRAAYQLPAGEQLPRVEIVYSHANMDAVPIEDAI 283 Query: 311 KRGAKGIVLAGVGDGNSSKGAIDALAAAAKQGVVVVRSSRVGSGFVNRNVEVEDDKLGFA 370 GAKGIVLAGVGDGN+SK A+DAL AAAK+G++VVRS+RV SGFV RNVEV+DDK GF Sbjct: 284 SHGAKGIVLAGVGDGNTSKPALDALEAAAKKGILVVRSTRVRSGFVTRNVEVDDDKNGFV 343 Query: 371 VSLDLNPQKARVLLELLIANGITEPSAVQQAF 402 VS DLNPQKARVL +LLIA+G+T P+ +Q+AF Sbjct: 344 VSEDLNPQKARVLTQLLIASGVTAPAELQRAF 375 >ref|ZP_01764925.1| L-asparaginase II [Burkholderia pseudomallei 305] ref|ZP_02452857.1| L-asparaginase [Burkholderia pseudomallei 91] ref|ZP_04521874.1| L-asparaginase (L-asparagine amidohydrolase) (L-ASNase) [Burkholderia pseudomallei MSHR346] ref|ZP_04891139.1| L-asparaginase, type II [Burkholderia pseudomallei 1655] gb|EBA50075.1| L-asparaginase II [Burkholderia pseudomallei 305] gb|EDU12123.1| L-asparaginase, type II [Burkholderia pseudomallei 1655] gb|EEP50788.1| L-asparaginase (L-asparagine amidohydrolase) (L-ASNase) [Burkholderia pseudomallei MSHR346] Length = 369 Score = 412 bits (1060), Expect = e-113, Method: Compositional matrix adjust. Identities = 208/355 (58%), Positives = 270/355 (76%), Gaps = 7/355 (1%) Query: 54 RLFLAAALTI---GIASPVSAQQATG---TTLARVLVLGTGGTIAGQASARAGNAYDSGK 107 R AA +T+ G + +AQQA+ L R+LVL TGGTIA + R+ Y++G Sbjct: 12 RGLHAALMTLTIGGFTTAANAQQASAPPPQNLPRILVLATGGTIASTSDPRSAIGYNAGG 71 Query: 108 VSAANLIAAIPGIDKLSQISAEQISSIGSQDMNDNVWFDLVKRIRAAVDNKEVDGVVITH 167 ++ L+A++PG+DK++ I AEQIS++GSQDMN +W+ L RI+ A+D EVDG+VITH Sbjct: 72 ITGDQLLASVPGLDKIASIKAEQISNVGSQDMNGKIWYQLAIRIKQAIDRNEVDGIVITH 131 Query: 168 GTDTMEETAFFLQNVLDTDVPIVLVGSMRPSTAIGADGPANLYEAMRVASAPESRGRGVL 227 GTDTMEET FFL +VL T P+VLVGSMRP + ADGP NLYEA VA++P+S RGVL Sbjct: 132 GTDTMEETGFFLDHVLSTSKPVVLVGSMRPGGSTSADGPMNLYEAAEVAASPQSVNRGVL 191 Query: 228 VVLNDTIHAARWVQKTNTTSVETFRSPDAGPVGYVDTGSLRFLQPAAPARGAKLKLPDAP 287 +V+NDTI++ RW KTNTTSV+TF++P+AGP+GYVD S+R+L PA + L+LP Sbjct: 192 MVMNDTINSPRWATKTNTTSVQTFQNPNAGPLGYVDAASIRYLSPAPTSHRKALELPSG- 250 Query: 288 PLPRVDIIYSHANMDAAEVEDAVKRGAKGIVLAGVGDGNSSKGAIDALAAAAKQGVVVVR 347 LPRVDI+Y+HA+MDA +++DAV+ AKGIVLAGVGDGN+SK A+DA+ AAA++G+VVVR Sbjct: 251 GLPRVDIVYAHADMDATQIQDAVQHDAKGIVLAGVGDGNASKAALDAMEAAARKGIVVVR 310 Query: 348 SSRVGSGFVNRNVEVEDDKLGFAVSLDLNPQKARVLLELLIANGITEPSAVQQAF 402 S+RV SGFVNRNVEV DDK GF S DLNPQK+RVL +LLIANG+T P VQ+AF Sbjct: 311 STRVSSGFVNRNVEVPDDKSGFVASYDLNPQKSRVLAQLLIANGVTSPDKVQKAF 365 >ref|YP_002909387.1| putative L-asparaginase [Burkholderia glumae BGR1] gb|ACR32152.1| Probable L-asparaginase [Burkholderia glumae BGR1] Length = 382 Score = 412 bits (1059), Expect = e-113, Method: Compositional matrix adjust. Identities = 213/326 (65%), Positives = 262/326 (80%) Query: 78 TLARVLVLGTGGTIAGQASARAGNAYDSGKVSAANLIAAIPGIDKLSQISAEQISSIGSQ 137 +L RV +L TGGTIAG+A+ARA NAYD+G + L+ A+PGI+KL+++ EQI+SIGSQ Sbjct: 54 SLPRVAILATGGTIAGKAAARAANAYDAGALGVRQLVDAVPGIEKLARLRPEQIASIGSQ 113 Query: 138 DMNDNVWFDLVKRIRAAVDNKEVDGVVITHGTDTMEETAFFLQNVLDTDVPIVLVGSMRP 197 DMND VWF L KRI+ D E D +VITHGTDT+EETAFFL NVL TD P+VLVGSMRP Sbjct: 114 DMNDAVWFKLAKRIQDISDRNEADAIVITHGTDTLEETAFFLDNVLHTDKPVVLVGSMRP 173 Query: 198 STAIGADGPANLYEAMRVASAPESRGRGVLVVLNDTIHAARWVQKTNTTSVETFRSPDAG 257 STAI ADGPANLYEA+ VA+ P +RGRGV++VLNDTIH+AR KTN+TSV+TF SP++G Sbjct: 174 STAISADGPANLYEAVEVAADPHARGRGVMIVLNDTIHSARGATKTNSTSVQTFASPNSG 233 Query: 258 PVGYVDTGSLRFLQPAAPARGAKLKLPDAPPLPRVDIIYSHANMDAAEVEDAVKRGAKGI 317 P+GYVD S+RFL PAA K LP LP V+I+YSH+NMDA +++ A+ KGI Sbjct: 234 PIGYVDPASVRFLAPAAADSRKKYVLPADGKLPDVEIVYSHSNMDARQIDHAIADHVKGI 293 Query: 318 VLAGVGDGNSSKGAIDALAAAAKQGVVVVRSSRVGSGFVNRNVEVEDDKLGFAVSLDLNP 377 VLAGVGDGN+S A+DAL AA+QG+VVVRS+RVGSGFVNRNVEV DD+ GFAVSLDLNP Sbjct: 294 VLAGVGDGNTSGKALDALQQAARQGIVVVRSTRVGSGFVNRNVEVNDDQRGFAVSLDLNP 353 Query: 378 QKARVLLELLIANGITEPSAVQQAFA 403 QKAR+L +LL+AN +T P+ VQ+AF+ Sbjct: 354 QKARILTQLLVANRVTSPAEVQRAFS 379 >ref|YP_112062.1| L-asparaginase [Burkholderia pseudomallei K96243] emb|CAH39538.1| L-asparaginase [Burkholderia pseudomallei K96243] Length = 351 Score = 412 bits (1058), Expect = e-113, Method: Compositional matrix adjust. Identities = 207/346 (59%), Positives = 267/346 (77%), Gaps = 5/346 (1%) Query: 61 LTIG-IASPVSAQQATG---TTLARVLVLGTGGTIAGQASARAGNAYDSGKVSAANLIAA 116 LTIG + +AQQA+ L R+LVL TGGTIA + R+ Y++G ++ L+A+ Sbjct: 3 LTIGGFTTAANAQQASAPPPQNLPRILVLATGGTIASTSDPRSAIGYNAGGITGDQLLAS 62 Query: 117 IPGIDKLSQISAEQISSIGSQDMNDNVWFDLVKRIRAAVDNKEVDGVVITHGTDTMEETA 176 +PG+DK++ I AEQIS++GSQDMN +W+ L RI+ A+D EVDG+VITHGTDTMEET Sbjct: 63 VPGLDKIASIKAEQISNVGSQDMNGKIWYQLAIRIKQAIDRNEVDGIVITHGTDTMEETG 122 Query: 177 FFLQNVLDTDVPIVLVGSMRPSTAIGADGPANLYEAMRVASAPESRGRGVLVVLNDTIHA 236 FFL +VL T P+VLVGSMRP + ADGP NLYEA VA++P+S RGVL+V+NDTI++ Sbjct: 123 FFLDHVLSTSKPVVLVGSMRPGGSTSADGPMNLYEAAEVAASPQSVNRGVLMVMNDTINS 182 Query: 237 ARWVQKTNTTSVETFRSPDAGPVGYVDTGSLRFLQPAAPARGAKLKLPDAPPLPRVDIIY 296 RW KTNTTSV+TF++P+AGP+GYVD S+R+L PA + L+LP LPRVDI+Y Sbjct: 183 PRWATKTNTTSVQTFQNPNAGPLGYVDAASIRYLSPAPTSHRKALELPSG-GLPRVDIVY 241 Query: 297 SHANMDAAEVEDAVKRGAKGIVLAGVGDGNSSKGAIDALAAAAKQGVVVVRSSRVGSGFV 356 +HA+MDA +++DAV+ AKGIVLAGVGDGN+SK A+DA+ AAA++G+VVVRS+RV SGFV Sbjct: 242 AHADMDATQIQDAVQHDAKGIVLAGVGDGNASKAALDAMEAAARKGIVVVRSTRVSSGFV 301 Query: 357 NRNVEVEDDKLGFAVSLDLNPQKARVLLELLIANGITEPSAVQQAF 402 NRNVEV DDK GF S DLNPQK+RVL +LLIANG+T P VQ+AF Sbjct: 302 NRNVEVPDDKSGFVASYDLNPQKSRVLAQLLIANGVTSPDKVQKAF 347 >ref|ZP_02487028.1| L-asparaginase [Burkholderia pseudomallei 7894] ref|ZP_02511227.1| L-asparaginase [Burkholderia pseudomallei BCC215] Length = 369 Score = 412 bits (1058), Expect = e-113, Method: Compositional matrix adjust. Identities = 208/355 (58%), Positives = 269/355 (75%), Gaps = 7/355 (1%) Query: 54 RLFLAAALTI---GIASPVSAQQATG---TTLARVLVLGTGGTIAGQASARAGNAYDSGK 107 R AA +T+ G + +AQQA+ L R+LVL TGGTIA R+ Y++G Sbjct: 12 RGLHAALMTLTIGGFTTAANAQQASAPPPQNLPRILVLATGGTIASTPHPRSAIGYNAGG 71 Query: 108 VSAANLIAAIPGIDKLSQISAEQISSIGSQDMNDNVWFDLVKRIRAAVDNKEVDGVVITH 167 ++ L+A++PG+DK++ I AEQIS++GSQDMN +W+ L RI+ A+D EVDG+VITH Sbjct: 72 ITGDQLLASVPGLDKIASIKAEQISNVGSQDMNGKIWYQLAIRIKQAIDRNEVDGIVITH 131 Query: 168 GTDTMEETAFFLQNVLDTDVPIVLVGSMRPSTAIGADGPANLYEAMRVASAPESRGRGVL 227 GTDTMEET FFL +VL T P+VLVGSMRP + ADGP NLYEA VA++P+S RGVL Sbjct: 132 GTDTMEETGFFLDHVLSTSKPVVLVGSMRPGGSTSADGPMNLYEAAEVAASPQSVNRGVL 191 Query: 228 VVLNDTIHAARWVQKTNTTSVETFRSPDAGPVGYVDTGSLRFLQPAAPARGAKLKLPDAP 287 +V+NDTI++ RW KTNTTSV+TF++P+AGP+GYVD S+R+L PA + L+LP Sbjct: 192 MVMNDTINSPRWATKTNTTSVQTFQNPNAGPLGYVDAASIRYLSPAPTSHRKALELPSG- 250 Query: 288 PLPRVDIIYSHANMDAAEVEDAVKRGAKGIVLAGVGDGNSSKGAIDALAAAAKQGVVVVR 347 LPRVDI+Y+HA+MDA +++DAV+ AKGIVLAGVGDGN+SK A+DA+ AAA++G+VVVR Sbjct: 251 GLPRVDIVYAHADMDATQIQDAVQHDAKGIVLAGVGDGNASKAALDAMEAAARKGIVVVR 310 Query: 348 SSRVGSGFVNRNVEVEDDKLGFAVSLDLNPQKARVLLELLIANGITEPSAVQQAF 402 S+RV SGFVNRNVEV DDK GF S DLNPQK+RVL +LLIANG+T P VQ+AF Sbjct: 311 STRVSSGFVNRNVEVPDDKSGFVASYDLNPQKSRVLAQLLIANGVTSPDKVQKAF 365