BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0059.0194.p (316 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_774501.1| rhizopine catabolism protein [Bradyrhizobium ja... 451 e-125 ref|YP_001202839.1| fatty acid desaturase [Bradyrhizobium sp. OR... 422 e-116 ref|YP_001243272.1| fatty acid desaturase [Bradyrhizobium sp. BT... 400 e-109 ref|YP_004613815.1| fatty acid desaturase [Mesorhizobium opportu... 203 4e-50 ref|YP_004143967.1| fatty acid desaturase [Mesorhizobium ciceri ... 197 2e-48 >ref|NP_774501.1| rhizopine catabolism protein [Bradyrhizobium japonicum USDA 110] dbj|BAC53126.1| bll7861 [Bradyrhizobium japonicum USDA 110] Length = 315 Score = 451 bits (1161), Expect = e-125, Method: Compositional matrix adjust. Identities = 225/313 (71%), Positives = 256/313 (81%) Query: 1 MSTAETSDVGPRLKPLSPLTLRDLSTKSNLAGAVRAASHYGMIVIVGALISLISSRYDLP 60 M+ A S+ G RLKPL+P LR+LS +S+L G ++ SHYG++V+VGAL+ +SS + + Sbjct: 1 MTDATVSEPGHRLKPLTPAMLRELSVRSDLRGTAKSLSHYGLVVLVGALVWKVSSAWGVL 60 Query: 61 WALPLIAVQGYFVAFLFMVVHETAHKTAFRSPVLNLVVGNLSAFMIGLPYQYYCLFHWDH 120 WALPL+AVQGYFVAFLFM +HETAHKTAFRS LNL VG S F+IG PY+YYCLFHWDH Sbjct: 61 WALPLMAVQGYFVAFLFMALHETAHKTAFRSRGLNLAVGYPSGFIIGQPYEYYCLFHWDH 120 Query: 121 HRYTQDPEKDPELIVGPKPASDTQLAIAYSGLLQVLVRIRLMFWHALTGKVVVPWIPEPK 180 HRYTQDPEKDPELIVG KP SDTQLA+AYSGLLQV R+ LM HA+TGKV VPWIPE K Sbjct: 121 HRYTQDPEKDPELIVGVKPKSDTQLALAYSGLLQVAGRLWLMLGHAVTGKVTVPWIPENK 180 Query: 181 RASIVLEARLYLAGYLLLLAASFALHSAILLWVWIVPLLAGQLILRPYLYAEHTGCERTR 240 RA IV EAR Y A Y LLLA S SA+LLWVWIVPL+ GQ LRPYLYAEHTGC+RTR Sbjct: 181 RAIIVREARAYAALYALLLALSLWFSSALLLWVWIVPLVIGQFFLRPYLYAEHTGCDRTR 240 Query: 241 SAFENTRTTMTGRIMKWFAWNMPYHVEHHAYPTVPFHALPKLNAIVDGHIVYRGSSYRAV 300 SAF+NTRTT TG ++KWFAWNMPYHVEHHAYP++PFHALPKLN IVDG IV+RG Y A Sbjct: 241 SAFQNTRTTYTGALVKWFAWNMPYHVEHHAYPSIPFHALPKLNEIVDGEIVHRGRGYIAT 300 Query: 301 TRETWAWFRRQRQ 313 TRETWAWFRR RQ Sbjct: 301 TRETWAWFRRHRQ 313 >ref|YP_001202839.1| fatty acid desaturase [Bradyrhizobium sp. ORS 278] emb|CAL74599.1| Putative fatty acid desaturase (DesA family) [Bradyrhizobium sp. ORS 278] Length = 316 Score = 422 bits (1086), Expect = e-116, Method: Compositional matrix adjust. Identities = 213/306 (69%), Positives = 242/306 (79%) Query: 6 TSDVGPRLKPLSPLTLRDLSTKSNLAGAVRAASHYGMIVIVGALISLISSRYDLPWALPL 65 T + PR K L+P L+ L +SNL GA+R SHYG I+ VGALI ++S L WALPL Sbjct: 3 TEEFAPRSKLLTPPVLQQLCARSNLKGALRTLSHYGAIIAVGALIGAVASSQGLLWALPL 62 Query: 66 IAVQGYFVAFLFMVVHETAHKTAFRSPVLNLVVGNLSAFMIGLPYQYYCLFHWDHHRYTQ 125 + VQG +AFLFM VHETAHKTAF S LN+ VGNLS F+IGLPY+YYCLFHW+HHR+TQ Sbjct: 63 LPVQGVLIAFLFMAVHETAHKTAFDSRRLNVAVGNLSGFLIGLPYEYYCLFHWEHHRHTQ 122 Query: 126 DPEKDPELIVGPKPASDTQLAIAYSGLLQVLVRIRLMFWHALTGKVVVPWIPEPKRASIV 185 DP +DPELIVGPKP S+TQLAIAYSGL+QV R+RLM HALTG+V VPW+P +RA IV Sbjct: 123 DPLRDPELIVGPKPRSETQLAIAYSGLVQVAGRLRLMLKHALTGEVTVPWVPAGRRAIIV 182 Query: 186 LEARLYLAGYLLLLAASFALHSAILLWVWIVPLLAGQLILRPYLYAEHTGCERTRSAFEN 245 EAR Y+AGY LLL S ALHSA+LL VW+VPLL GQLILRPYLYAEHTGC+ TRSAF N Sbjct: 183 REARFYVAGYALLLIGSLALHSALLLCVWVVPLLLGQLILRPYLYAEHTGCDHTRSAFHN 242 Query: 246 TRTTMTGRIMKWFAWNMPYHVEHHAYPTVPFHALPKLNAIVDGHIVYRGSSYRAVTRETW 305 TRTT T + W AWNMPYHVEHHAYP VPFHALPKL+AIVDG I YRG Y AVTR+TW Sbjct: 243 TRTTFTTPFVHWLAWNMPYHVEHHAYPAVPFHALPKLHAIVDGEIKYRGEGYVAVTRQTW 302 Query: 306 AWFRRQ 311 WFR Q Sbjct: 303 TWFRTQ 308 >ref|YP_001243272.1| fatty acid desaturase [Bradyrhizobium sp. BTAi1] gb|ABQ39366.1| Putative fatty acid desaturase (DesA family) [Bradyrhizobium sp. BTAi1] Length = 311 Score = 400 bits (1027), Expect = e-109, Method: Compositional matrix adjust. Identities = 206/306 (67%), Positives = 245/306 (80%) Query: 6 TSDVGPRLKPLSPLTLRDLSTKSNLAGAVRAASHYGMIVIVGALISLISSRYDLPWALPL 65 T PRLK L+P L++L +S+L GA+R HYG I++VG LI+ I+S + + WALPL Sbjct: 3 TDAFAPRLKLLTPPVLQELCRRSDLQGALRTLGHYGAIIVVGILIAWIASTHGVLWALPL 62 Query: 66 IAVQGYFVAFLFMVVHETAHKTAFRSPVLNLVVGNLSAFMIGLPYQYYCLFHWDHHRYTQ 125 + VQG VAFLFM VHETAHKTAF S LN+V+GNLS F+IGLPY YYCLFHW+HHR+TQ Sbjct: 63 LPVQGVLVAFLFMAVHETAHKTAFDSRRLNVVIGNLSGFLIGLPYDYYCLFHWEHHRHTQ 122 Query: 126 DPEKDPELIVGPKPASDTQLAIAYSGLLQVLVRIRLMFWHALTGKVVVPWIPEPKRASIV 185 DP++DPELIVGPKP S+TQLAIAYSGL+QV R+RL+ HA+TG+V VPW+P +RA+IV Sbjct: 123 DPQRDPELIVGPKPRSETQLAIAYSGLVQVAGRLRLVLKHAVTGEVTVPWVPAGRRAAIV 182 Query: 186 LEARLYLAGYLLLLAASFALHSAILLWVWIVPLLAGQLILRPYLYAEHTGCERTRSAFEN 245 EAR Y+AGY LLL S LHS +LLWVW++PLL GQLILRPYLYAEHTGC+ TR AF N Sbjct: 183 REARAYVAGYALLLIGSLVLHSTLLLWVWVLPLLLGQLILRPYLYAEHTGCDHTRRAFHN 242 Query: 246 TRTTMTGRIMKWFAWNMPYHVEHHAYPTVPFHALPKLNAIVDGHIVYRGSSYRAVTRETW 305 TRTT+T R + W WNMPYHVEHHAYP VPFHALP+L+AIVD I YRG Y AVTR+TW Sbjct: 243 TRTTLTTRAVHWLTWNMPYHVEHHAYPAVPFHALPRLHAIVDDEIKYRGQGYIAVTRQTW 302 Query: 306 AWFRRQ 311 AWFR Q Sbjct: 303 AWFRAQ 308 >ref|YP_004613815.1| fatty acid desaturase [Mesorhizobium opportunistum WSM2075] gb|AEH89721.1| fatty acid desaturase [Mesorhizobium opportunistum WSM2075] Length = 296 Score = 203 bits (516), Expect = 4e-50, Method: Compositional matrix adjust. Identities = 114/277 (41%), Positives = 169/277 (61%), Gaps = 11/277 (3%) Query: 16 LSPLTLRDLSTKSNLAGAVRAASHYGMIVIVGALISLISSRYDLPWALPLIAVQGYFVAF 75 L+P L+ KS++AG V+ +H+G I+++GALI+ +P+ L+ QG + F Sbjct: 10 LTPEERNRLTAKSDVAGLVQLGAHWGAIMVIGALIAA-----KVPFWPLLMLPQGILIVF 64 Query: 76 LFMVVHETAHKTAFRSPVLNLVVGNLSAFMIGLPYQYYCLFHWDHHRYTQDPEKDPELIV 135 LF ++HET H+TAF + LN V + + I LP ++ FH+ HHR+TQDP+ DPEL Sbjct: 65 LFTLLHETVHRTAFETQRLNDAVARVCSLAIALPADWFRYFHFAHHRFTQDPDNDPEL-A 123 Query: 136 GPKPASDTQLAIAYSGLLQVLVRIRLMFWHALTGKVVVPWIPEPKRASIVLEARLYLAGY 195 PKP + Q + SG+ + + +A+ G+ ++P R + EARL +A Y Sbjct: 124 SPKPETLRQYLVHVSGIPVWWGHFKTLHANAI-GRSQDSYVPPKGRPKVRTEARLMIAIY 182 Query: 196 --LLLLAASFALHSAILLWVWIVPLLAGQLILRPYLYAEHTGCERTRSAFENTRTTMTGR 253 ++LLA F L ++LL+VWIVP L GQ LR YL AEH C + ENTRTT+T Sbjct: 183 AVVILLAVYFRL--SVLLYVWIVPALLGQPFLRLYLLAEHGRCPFVANMLENTRTTLTNW 240 Query: 254 IMKWFAWNMPYHVEHHAYPTVPFHALPKLNAIVDGHI 290 +++ AWNMP+H EHHAYP VPFH LP+ +A+++ H+ Sbjct: 241 LVRKLAWNMPFHAEHHAYPGVPFHQLPRFHALIERHL 277 >ref|YP_004143967.1| fatty acid desaturase [Mesorhizobium ciceri biovar biserrulae WSM1271] gb|ADV13917.1| fatty acid desaturase [Mesorhizobium ciceri biovar biserrulae WSM1271] Length = 296 Score = 197 bits (500), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 113/269 (42%), Positives = 166/269 (61%), Gaps = 11/269 (4%) Query: 24 LSTKSNLAGAVRAASHYGMIVIVGALISLISSRYDLPWALPLIAVQGYFVAFLFMVVHET 83 L+ KS+ AG V+ A+H+G IVI+G+LI+ +P+ L+ QG + FLF ++HET Sbjct: 18 LTAKSDAAGLVQLAAHWGAIVILGSLIAA-----KVPFWPLLMLPQGLLIVFLFTLLHET 72 Query: 84 AHKTAFRSPVLNLVVGNLSAFMIGLPYQYYCLFHWDHHRYTQDPEKDPELIVGPKPASDT 143 H+TAF + LN +V + + I LP ++ FH+ HHRYTQDP+ DPEL PKP + Sbjct: 73 VHRTAFEAQRLNDLVARVCSLAIALPADWFRYFHFAHHRYTQDPDNDPELAF-PKPETWR 131 Query: 144 QLAIAYSGLLQVLVRIRLMFWHALTGKVVVPWIPEPKRASIVLEARLYLAGY--LLLLAA 201 Q + SG+ I+ ++ +A + ++P + +EAR +A Y LLLLA Sbjct: 132 QYLVHISGIPLWWGHIKTLYANA-ADRGRESYVPPKGYDKVRIEARAMIAFYAVLLLLAV 190 Query: 202 SFALHSAILLWVWIVPLLAGQLILRPYLYAEHTGCERTRSAFENTRTTMTGRIMKWFAWN 261 F L ++L++VWI+P L GQ LR YL AEH C + ENTRTT+T +++ AWN Sbjct: 191 YFRL--SVLVYVWILPALLGQPFLRLYLLAEHGRCPFVANMLENTRTTLTNWLVRKLAWN 248 Query: 262 MPYHVEHHAYPTVPFHALPKLNAIVDGHI 290 MP+H EHHAYP VPFH LP+ +A+++ H+ Sbjct: 249 MPFHAEHHAYPGVPFHQLPRFHALIERHL 277