BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0059.0184.p (350 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_774221.1| dehydrogenase [Bradyrhizobium japonicum USDA 11... 638 0.0 ref|NP_085760.1| malate dehydrogenase [Mesorhizobium loti MAFF30... 533 e-149 ref|ZP_01853790.1| putative dehydrogenase [Planctomyces maris DS... 247 2e-63 ref|YP_004270360.1| malate dehydrogenase [Planctomyces brasilien... 246 3e-63 ref|YP_003629389.1| malate/L-lactate dehydrogenase [Planctomyces... 239 4e-61 >ref|NP_774221.1| dehydrogenase [Bradyrhizobium japonicum USDA 110] dbj|BAC52846.1| blr7581 [Bradyrhizobium japonicum USDA 110] Length = 350 Score = 638 bits (1646), Expect = 0.0, Method: Compositional matrix adjust. Identities = 304/349 (87%), Positives = 328/349 (93%) Query: 1 MPIVQADRLTRIGAALLKGAGASEEEASAVASGCVNANLAGHDSHGVIAIPTYIDRIKAG 60 MPIV+ADRLTRI AALL+ AGAS+EEA AVA+GCVNANLAGHDSHGVIA+PTYIDRIKAG Sbjct: 1 MPIVRADRLTRISAALLQAAGASQEEADAVATGCVNANLAGHDSHGVIAVPTYIDRIKAG 60 Query: 61 HIVPGAKWTIVQETPTTTVIDGHWGFGFHVNAKAMEMTINKAKTANVAACTVFRQSHVGR 120 HIVPGA WTI+QE+PTTTVIDGHWGFGFHVNAKAM +TI KAKTANVAACTVFRQSHVGR Sbjct: 61 HIVPGATWTIIQESPTTTVIDGHWGFGFHVNAKAMALTIEKAKTANVAACTVFRQSHVGR 120 Query: 121 LAHYPMMAMREGMIGIAAADSGRSPKHVAPFGGREARLGTNPLSIAVPSDLDAPFYLDMA 180 LA YP+MAMREGMIGIA ADSGRSPKHVAPFGG+EARLGTNP+SIAVPSDLDAPFYLDMA Sbjct: 121 LAAYPLMAMREGMIGIATADSGRSPKHVAPFGGKEARLGTNPISIAVPSDLDAPFYLDMA 180 Query: 181 TSAVAAGKIQLAVARGESIPKGWIIDAEGRDTTDPADYRKGGALLPLGGSEGYKGSGLAA 240 TSAVAAGKIQLAVARG+ IP GWIIDAEGR T DP YRKGGALLPLGG+EGYKGSGLAA Sbjct: 181 TSAVAAGKIQLAVARGDKIPTGWIIDAEGRHTNDPTQYRKGGALLPLGGTEGYKGSGLAA 240 Query: 241 MVEVLCGLLTGLGFGVEPTGRHNDGCFMAVFNVAAFRPLKDFRKEVADFARYLKSTPPSE 300 MVEVLCGLLTGLGFGVEPTGRHNDGCFMAVFNVAAFRPLK+F++EVA+FARYLKSTPPSE Sbjct: 241 MVEVLCGLLTGLGFGVEPTGRHNDGCFMAVFNVAAFRPLKEFKREVAEFARYLKSTPPSE 300 Query: 301 GSRGVFYPGEIEYLREQQRRKDGIEIEDATWDKLKALAADYKLTDQLDL 349 GS GV+YPGEIE +REQQR +DGIE+EDATWDKL+ALA +Y+L LDL Sbjct: 301 GSTGVYYPGEIEGVREQQRLRDGIEVEDATWDKLRALAREYRLDTVLDL 349 >ref|NP_085760.1| malate dehydrogenase [Mesorhizobium loti MAFF303099] dbj|BAB54601.1| probable malate dehydrogenase [Mesorhizobium loti MAFF303099] Length = 366 Score = 533 bits (1373), Expect = e-149, Method: Compositional matrix adjust. Identities = 259/347 (74%), Positives = 294/347 (84%) Query: 3 IVQADRLTRIGAALLKGAGASEEEASAVASGCVNANLAGHDSHGVIAIPTYIDRIKAGHI 62 +V++DRLTRI AALLK AGASE+EA AVA+GCVNANLAGHDSHGVIAIP YID +KAG I Sbjct: 19 VVKSDRLTRICAALLKAAGASEDEAQAVATGCVNANLAGHDSHGVIAIPGYIDSLKAGDI 78 Query: 63 VPGAKWTIVQETPTTTVIDGHWGFGFHVNAKAMEMTINKAKTANVAACTVFRQSHVGRLA 122 VPGA+WTIV+E+PTT VIDG+WGFGFHVNAKAM + I KAK ANVAACTVFRQSHVGRLA Sbjct: 79 VPGAEWTIVRESPTTAVIDGNWGFGFHVNAKAMALVIEKAKIANVAACTVFRQSHVGRLA 138 Query: 123 HYPMMAMREGMIGIAAADSGRSPKHVAPFGGREARLGTNPLSIAVPSDLDAPFYLDMATS 182 YP+MA+ GMIG A A SG P VAPFGGREARLGTNP+SI VPS+L APF LDMATS Sbjct: 139 AYPLMAVSAGMIGFAVAGSGSFPDCVAPFGGREARLGTNPISIGVPSNLQAPFLLDMATS 198 Query: 183 AVAAGKIQLAVARGESIPKGWIIDAEGRDTTDPADYRKGGALLPLGGSEGYKGSGLAAMV 242 A AAGK+++A+AR E IP+GW+IDAEGR TT+P DY KGG LLPLGG EGYKGSGLAA+V Sbjct: 199 AAAAGKLEVAIARREKIPEGWLIDAEGRPTTNPHDYLKGGVLLPLGGPEGYKGSGLAAIV 258 Query: 243 EVLCGLLTGLGFGVEPTGRHNDGCFMAVFNVAAFRPLKDFRKEVADFARYLKSTPPSEGS 302 E+LCGLL GFG +P+GR N GCFMAVFNVAAF PL F++EVA+FARYLKS PSEGS Sbjct: 259 ELLCGLLPDPGFGPQPSGRENLGCFMAVFNVAAFCPLNKFKQEVAEFARYLKSATPSEGS 318 Query: 303 RGVFYPGEIEYLREQQRRKDGIEIEDATWDKLKALAADYKLTDQLDL 349 GVFYPGEI YLR Q+R +GIEIE ATWDKL+ LA +Y+L +LDL Sbjct: 319 PGVFYPGEIGYLRAQERMANGIEIEKATWDKLRTLARNYRLAGELDL 365 >ref|ZP_01853790.1| putative dehydrogenase [Planctomyces maris DSM 8797] gb|EDL60485.1| putative dehydrogenase [Planctomyces maris DSM 8797] Length = 381 Score = 247 bits (630), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 136/343 (39%), Positives = 187/343 (54%), Gaps = 3/343 (0%) Query: 1 MPIVQADRLTRIGAALLKGAGASEEEASAVASGCVNANLAGHDSHGVIAIPTYIDRIKAG 60 +P++ A L +LL GA+EEEA V+ V+ANL GHDSHGV+ +P Y+ R+K G Sbjct: 29 VPVISAQSLQNFTESLLLKGGANEEEARIVSHSLVDANLLGHDSHGVMRLPFYLGRVKEG 88 Query: 61 HIVPGAKWTIVQETPTTTVIDGHWGFGFHVNAKAMEMTINKAKTANVAACTVFRQSHVGR 120 + G K I+ ETP DG WGFG V M I KA T V+ T+ R SH+GR Sbjct: 89 ILKAGEKLQILNETPAAITGDGCWGFGQTVMHDLMNRLIEKAGTLGVSCGTLKRASHIGR 148 Query: 121 LAHYPMMAMREGMIGIAAADSGRSPKHVAPFGGREARLGTNPLSIAVPSDLDAPFYLDMA 180 L Y MA +GM I A++ S VAP GG+ RLGTNP+ I +P + PF LD Sbjct: 149 LGEYAEMAAAKGMASIICANTHGSAPRVAPVGGKRPRLGTNPICIGMPGGAEGPFVLDFG 208 Query: 181 TSAVAAGKIQLAVARGESIPKGWIIDAEGRDTTDPADYR--KGGALLPLGGSEGYKGSGL 238 TSA A GK+++ GE +P G I+D +G TTDP G +LP+GG + YKG GL Sbjct: 209 TSATAEGKVRIKKIAGEQVPPGLILDPDGNPTTDPNMLYGDPPGTILPMGGDQAYKGFGL 268 Query: 239 AAMVEVLCGLLTGLGFGV-EPTGRHNDGCFMAVFNVAAFRPLKDFRKEVADFARYLKSTP 297 + MVE+LCG L+G +P + F+ V + E+++ +Y++S P Sbjct: 269 SFMVEMLCGALSGGQCAFPDPPPPQGNCVFVVVIDPGHLGGQNHLMNEISNLEKYVRSVP 328 Query: 298 PSEGSRGVFYPGEIEYLREQQRRKDGIEIEDATWDKLKALAAD 340 EG VF PG+ E R+ GI ++ W+ L LAA+ Sbjct: 329 LKEGISEVFLPGDPEKKTAATRKTSGISLDKGNWEALSNLAAE 371 >ref|YP_004270360.1| malate dehydrogenase [Planctomyces brasiliensis DSM 5305] gb|ADY60338.1| Malate dehydrogenase [Planctomyces brasiliensis DSM 5305] Length = 348 Score = 246 bits (629), Expect = 3e-63, Method: Compositional matrix adjust. Identities = 133/346 (38%), Positives = 195/346 (56%), Gaps = 4/346 (1%) Query: 1 MPIVQADRLTRIGAALLKGAGASEEEASAVASGCVNANLAGHDSHGVIAIPTYIDRIKAG 60 MP+ D L R+G +L AGA+ +EAS VA +ANL GHDSHGV+ + Y+D I+ G Sbjct: 1 MPVFSIDELRRLGTDILAAAGATPDEASVVAEELASANLVGHDSHGVMRLVQYVDFIRNG 60 Query: 61 HIVPGAKWTIVQETPTTTVIDGHWGFGFHVNAKAMEMTINKAKTANVAACTVFRQSHVGR 120 HIVPG + IV+E V+D H+ FG + A E KAK A + +HVGR Sbjct: 61 HIVPGRQPEIVREGAAFLVVDAHFSFGQVAASWAFERLQQKAKDCGCANAFIRNCNHVGR 120 Query: 121 LAHYPMMAMREGMIGIAAADSGRSPKHVAPFGGREARLGTNPLSIAVPSDLDAPFYLDMA 180 L Y A EG + + ++ P VAPFG E R+GTNP+S+A P ++ P LDM Sbjct: 121 LGSYTEKAAEEGFAALMSVNA-PGPGQVAPFGAMERRMGTNPISMASPG-VEFPHVLDMT 178 Query: 181 TSAVAAGKIQLAVARGESIPKGWIIDAEGRDTTDPADY--RKGGALLPLGGSEGYKGSGL 238 TSA A GK+++A G ++P+GW++D G + +P DY G +LPLGG +KG GL Sbjct: 179 TSATAEGKLRVAHQSGLTVPEGWMLDGHGNPSCNPGDYYNEPNGCILPLGGPLAHKGFGL 238 Query: 239 AAMVEVLCGLLTGLGFGVEPTGRHNDGCFMAVFNVAAFRPLKDFRKEVADFARYLKSTPP 298 + MV+V CG+L+G G G R +G ++ + ++ ++ K + + ++KS Sbjct: 239 STMVDVFCGILSGSGVGRTDLPRGANGVWIELIDIEQVVGRDEYDKWMGQYETHIKSAKK 298 Query: 299 SEGSRGVFYPGEIEYLREQQRRKDGIEIEDATWDKLKALAADYKLT 344 G V +PGE+E E R +DG+ I D TW +L LA + ++ Sbjct: 299 RPGVEDVLFPGEVEKRTEVSRLQDGVNIPDETWRQLSELARELQVN 344 >ref|YP_003629389.1| malate/L-lactate dehydrogenase [Planctomyces limnophilus DSM 3776] gb|ADG67190.1| Malate/L-lactate dehydrogenase [Planctomyces limnophilus DSM 3776] Length = 353 Score = 239 bits (611), Expect = 4e-61, Method: Compositional matrix adjust. Identities = 128/341 (37%), Positives = 195/341 (57%), Gaps = 5/341 (1%) Query: 1 MPIVQADRLTRIGAALLKGAGASEEEASAVASGCVNANLAGHDSHGVIAIPTYIDRIKAG 60 MP++ + L+ L AG SE +AS +A+ V ANL GHDSHGV+ + +YI++I+ G Sbjct: 1 MPVIAIETLSSYAQKLFLTAGVSEADASCMATSLVEANLRGHDSHGVMRVASYIEQIEQG 60 Query: 61 HIVPGAKWTIVQETPTTTVIDGHWGFGFHVNAKAMEMTINKAKTANVAACTVFRQSHVGR 120 I+P ++ IV+E+ ++ V DG+WG G + ++ I KA ++ V T+ R SH+GR Sbjct: 61 RILPKSQLDIVKESASSIVADGNWGLGLVLAHHLVDKMIAKAASSAVVIGTLRRSSHIGR 120 Query: 121 LAHYPMMAMREGMIGIAAADSGRSPKHVAPFGGREARLGTNPLSIAVPSDLDAPFYLDMA 180 L Y G++ +A A++ + + VAP GG+ RLGTNP+ I VP PF D+ Sbjct: 121 LGEYAEKFAAHGLVSMALANTHGAAQRVAPVGGKRPRLGTNPICIGVPGGAQGPFIFDIG 180 Query: 181 TSAVAAGKIQLAVARGESIPKGWIIDAEGRDTTDPADY--RKGGALLPLGGSEGYKGSGL 238 TSA A GK+++ GE +P GWI+D EG +T+P D G +LPLGG + YKG GL Sbjct: 181 TSATAEGKVRVKKIAGEPVPPGWILDPEGNPSTNPNDLYGNPPGTILPLGGDQAYKGFGL 240 Query: 239 AAMVEVLCGLLTG--LGFGVEPTGRHNDGCFMAVFNVAAFRPLKDFRKEVADFARYLKST 296 A M+E+LCG ++G + P N G FMA+ + F +EV +Y++S Sbjct: 241 AFMIEMLCGGMSGGQCAYPNPPAPIGNCGWFMAI-SPEFFAGTDHLLQEVQQLEQYVRSV 299 Query: 297 PPSEGSRGVFYPGEIEYLREQQRRKDGIEIEDATWDKLKAL 337 P + S+ ++ PG+ E R+ GI +++ W L AL Sbjct: 300 PKIDESKEIYLPGDPERSTLNSRQAKGIPLDEGNWKALVAL 340