BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0059.0132.p (335 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_770522.1| hypothetical protein blr3882 [Bradyrhizobium ja... 462 e-128 ref|YP_001203593.1| ribonuclease Z [Bradyrhizobium sp. ORS 278] ... 440 e-121 ref|YP_004173135.1| ribonuclease Z [Anaerolinea thermophila UNI-... 267 2e-69 ref|YP_002297007.1| ribonuclease Z, putative [Rhodospirillum cen... 223 5e-56 ref|ZP_05974925.1| ribonuclease Z [Methanobrevibacter smithii DS... 204 2e-50 >ref|NP_770522.1| hypothetical protein blr3882 [Bradyrhizobium japonicum USDA 110] sp|Q89NF8.1|RNZ_BRAJA RecName: Full=Ribonuclease Z; Short=RNase Z; AltName: Full=tRNA 3 endonuclease; AltName: Full=tRNase Z dbj|BAC49147.1| blr3882 [Bradyrhizobium japonicum USDA 110] Length = 301 Score = 462 bits (1188), Expect = e-128, Method: Compositional matrix adjust. Identities = 233/301 (77%), Positives = 262/301 (87%) Query: 35 MFSLTFLGTSASVPSPERNHPGILVEAAGSRILVDCGEGTQRQLLRSGAGFRRLDRLLLT 94 MF+LTFLGTSASVPS ERNHP +LVEAAG RIL+DCGEGTQRQLLRSGAGFRRLDR+LLT Sbjct: 1 MFALTFLGTSASVPSAERNHPALLVEAAGKRILIDCGEGTQRQLLRSGAGFRRLDRILLT 60 Query: 95 HGHFDHVLGIPGLFSTLRLRQSSDAMTIHAGPGTLDVVVRMLAGLWGEGRAPIPLDLVPL 154 H H DHVLGIPGLFSTL LRQSSD MTIH GPGTLD+V+RMLAGLWG GRAPIP++ L Sbjct: 61 HAHLDHVLGIPGLFSTLGLRQSSDVMTIHGGPGTLDIVIRMLAGLWGAGRAPIPVEFAAL 120 Query: 155 SEGRVLDAGEFTIDCFAVRHRDTDSFAFSFQSAMRRHLLPDRLLALRVPDGPVRRELAEG 214 SEG+V+DAG FTIDCF VRHRDTDSF F FQS RRHLLPDRL +L VPDGP+R ELA+G Sbjct: 121 SEGQVIDAGGFTIDCFPVRHRDTDSFGFVFQSPARRHLLPDRLASLGVPDGPLRGELAQG 180 Query: 215 RQVKLDDGRTIDPEQVLGSAEGRKKLVIVGDAETTEGLAELVRDADVLVIEATFLERDAA 274 R V ++DGRTIDPE VLG A G +KLV++GD ETTEGL++ V DAD+LVIEATFL+RDA+ Sbjct: 181 RPVVIEDGRTIDPEDVLGPATGGRKLVVIGDTETTEGLSQYVADADMLVIEATFLDRDAS 240 Query: 275 IARDYGHLTASEAAALAARSGVKQLILTHISGRYPDEDILAEATRIFPNSRIAIDFDRIA 334 IARDYGHLTA+EAAA AA + V QL+LTH+SGRY DE+ILAEA RIFPNSRIA DFD I Sbjct: 241 IARDYGHLTAAEAAAFAAANNVGQLVLTHMSGRYEDEEILAEAARIFPNSRIAADFDHIV 300 Query: 335 I 335 + Sbjct: 301 V 301 >ref|YP_001203593.1| ribonuclease Z [Bradyrhizobium sp. ORS 278] sp|A4YN80.1|RNZ_BRASO RecName: Full=Ribonuclease Z; Short=RNase Z; AltName: Full=tRNA 3 endonuclease; AltName: Full=tRNase Z emb|CAL75356.1| Ribonuclease Z (EC 3.1.26.11) (RNase Z) (tRNase Z) (tRNA 3 endonuclease) [Bradyrhizobium sp. ORS 278] Length = 301 Score = 440 bits (1131), Expect = e-121, Method: Compositional matrix adjust. Identities = 224/301 (74%), Positives = 255/301 (84%) Query: 35 MFSLTFLGTSASVPSPERNHPGILVEAAGSRILVDCGEGTQRQLLRSGAGFRRLDRLLLT 94 MF L FLGTSASVPS +RNHPG+LV+A G RILVDCGEG QRQLL SGAGFRRLDR+LLT Sbjct: 1 MFELIFLGTSASVPSHDRNHPGLLVQAGGQRILVDCGEGIQRQLLASGAGFRRLDRILLT 60 Query: 95 HGHFDHVLGIPGLFSTLRLRQSSDAMTIHAGPGTLDVVVRMLAGLWGEGRAPIPLDLVPL 154 HGH DHVLGIPGLFSTLRLR+S+D M++H PGT+DVV+RMLAGLWG+GRAPIPL+LVPL Sbjct: 61 HGHLDHVLGIPGLFSTLRLRRSADVMSVHGSPGTIDVVIRMLAGLWGDGRAPIPLELVPL 120 Query: 155 SEGRVLDAGEFTIDCFAVRHRDTDSFAFSFQSAMRRHLLPDRLLALRVPDGPVRRELAEG 214 + G+VLDAG FTI+CFAVRHRDTDSF F F S RRHLLP+RL AL VPDGP+R+ LA+G Sbjct: 121 TPGQVLDAGAFTINCFAVRHRDTDSFGFEFVSPARRHLLPERLAALAVPDGPIRKTLADG 180 Query: 215 RQVKLDDGRTIDPEQVLGSAEGRKKLVIVGDAETTEGLAELVRDADVLVIEATFLERDAA 274 V LDDGR + PE VLG+ G KKLVIVGD ETT+GL VR AD+LVIEATFL+RD+A Sbjct: 181 YPVTLDDGRIVTPEDVLGTPGGGKKLVIVGDTETTDGLQAHVRGADLLVIEATFLQRDSA 240 Query: 275 IARDYGHLTASEAAALAARSGVKQLILTHISGRYPDEDILAEATRIFPNSRIAIDFDRIA 334 ARDYGHLTA+EAAALAA V QL+L HISGRYPDE+ILAEA IFP +RIA DFDR+ Sbjct: 241 TARDYGHLTAAEAAALAASGNVGQLVLNHISGRYPDEEILAEARSIFPATRIASDFDRLT 300 Query: 335 I 335 + Sbjct: 301 V 301 >ref|YP_004173135.1| ribonuclease Z [Anaerolinea thermophila UNI-1] dbj|BAJ62535.1| ribonuclease Z [Anaerolinea thermophila UNI-1] Length = 306 Score = 267 bits (683), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 142/302 (47%), Positives = 193/302 (63%), Gaps = 1/302 (0%) Query: 35 MFSLTFLGTSASVPSPERNHPGILVEAAGSRILVDCGEGTQRQLLRSGAGFRRLDRLLLT 94 MF +TFLGTSAS PS +R LV+ R L+DCGEGTQRQ+L++G GF+RL+ +L+T Sbjct: 1 MFEITFLGTSASAPSVKRGLSAQLVKHNEYRFLIDCGEGTQRQILQAGLGFKRLNHILIT 60 Query: 95 HGHFDHVLGIPGLFSTLRLRQSSDAMTIHAGPGTLDVVVRMLAGLWGEG-RAPIPLDLVP 153 HGH DH+LG+ GL ST ++ D + I+ L+ + +L + G R P+PL L+P Sbjct: 61 HGHLDHILGLAGLLSTFMRWETIDHLEIYGPRSALERIDDLLTRVVLRGARPPMPLTLIP 120 Query: 154 LSEGRVLDAGEFTIDCFAVRHRDTDSFAFSFQSAMRRHLLPDRLLALRVPDGPVRRELAE 213 ++ G + +F + F V HR ++S + F+ RR LP++ AL VP GP+RR+L E Sbjct: 121 ITPGVFFEGDDFRVSAFPVHHRGSESLGYLFEERSRRPFLPEKADALGVPAGPLRRDLVE 180 Query: 214 GRQVKLDDGRTIDPEQVLGSAEGRKKLVIVGDAETTEGLAELVRDADVLVIEATFLERDA 273 GR V L DGRTI P+ VLG +LV VGD TE L +V AD LVIEAT+LE + Sbjct: 181 GRAVHLPDGRTITPDMVLGEERPGTRLVHVGDCGETESLLGVVSGADGLVIEATYLEEER 240 Query: 274 AIARDYGHLTASEAAALAARSGVKQLILTHISGRYPDEDILAEATRIFPNSRIAIDFDRI 333 +A + HLTA AA LA R+GVK L LTH+S RY ++D+LAEA +FPN+ + DFD Sbjct: 241 EMAHQFSHLTARMAAELAQRAGVKALYLTHLSRRYREKDVLAEAQSVFPNTIVVRDFDTF 300 Query: 334 AI 335 I Sbjct: 301 II 302 >ref|YP_002297007.1| ribonuclease Z, putative [Rhodospirillum centenum SW] sp|B6IRV8.1|RNZ_RHOCS RecName: Full=Ribonuclease Z; Short=RNase Z; AltName: Full=tRNA 3 endonuclease; AltName: Full=tRNase Z gb|ACI98194.1| ribonuclease Z, putative [Rhodospirillum centenum SW] Length = 308 Score = 223 bits (567), Expect = 5e-56, Method: Compositional matrix adjust. Identities = 132/302 (43%), Positives = 169/302 (55%), Gaps = 1/302 (0%) Query: 35 MFSLTFLGTSASVPSPERNHPGILVEAAGSRILVDCGEGTQRQLLRSGAGFRRLDRLLLT 94 MF LTFLGT A+VPS R +LVE R LVDCGEGT RQ+ + G G RRLDR+LLT Sbjct: 1 MFQLTFLGTGATVPSARRGLSALLVEHETDRFLVDCGEGTLRQIRQGGVGVRRLDRILLT 60 Query: 95 HGHFDHVLGIPGLFSTLRLRQSSDAMTIHAGPGTLDVVVRMLAGL-WGEGRAPIPLDLVP 153 HGH DH+LG+ L TL L +D +T++ L + +L + W + + + Sbjct: 61 HGHLDHILGLASLVGTLDLWDQADRLTLYGSEEALHIARVLLDQVVWPDDHPGLDVIYAT 120 Query: 154 LSEGRVLDAGEFTIDCFAVRHRDTDSFAFSFQSAMRRHLLPDRLLALRVPDGPVRRELAE 213 L GR + G + F V H +T SF F F+ R L RL AL VP+G R ELA Sbjct: 121 LKPGRFCETGGVAVTAFPVCHHETQSFGFRFEETEHRPLDGARLDALGVPEGRERHELAH 180 Query: 214 GRQVKLDDGRTIDPEQVLGSAEGRKKLVIVGDAETTEGLAELVRDADVLVIEATFLERDA 273 V L DGRT+ E LG AE L +VGD + L E VR D+LVIEAT+ D Sbjct: 181 AGAVTLADGRTVRREDALGPAERGTSLAVVGDCGDADALVESVRGVDLLVIEATYRTADE 240 Query: 274 AIARDYGHLTASEAAALAARSGVKQLILTHISGRYPDEDILAEATRIFPNSRIAIDFDRI 333 A AR HLT ++A LA +GV +L+LTH S RY ++ AEA F +A DFDR+ Sbjct: 241 ATARSRSHLTVADACRLAQAAGVGRLVLTHQSDRYDPAEVEAEARARFEPVMVAKDFDRV 300 Query: 334 AI 335 + Sbjct: 301 EV 302 >ref|ZP_05974925.1| ribonuclease Z [Methanobrevibacter smithii DSM 2374] gb|EFC94170.1| ribonuclease Z [Methanobrevibacter smithii DSM 2374] Length = 300 Score = 204 bits (519), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 112/299 (37%), Positives = 174/299 (58%), Gaps = 5/299 (1%) Query: 38 LTFLGTSASVPSPERNHPGILVEAAGSRILVDCGEGTQRQLLRSGAGFRRLDRLLLTHGH 97 +TFLGTS++V S RNHPGI+++A G +L DCGE TQRQL+ + ++ ++ ++H H Sbjct: 3 ITFLGTSSAVHSYTRNHPGIILKAFGEVMLFDCGESTQRQLIYAKISPMKISKIFISHYH 62 Query: 98 FDHVLGIPGLFSTLRLRQSSDAMTIHAGPGTLDVVVRMLAGLWGEGRAPIPLDLVPLSEG 157 DH+LG+PGL +L R +TI+ GP L V + L G + P++ + +S G Sbjct: 63 GDHILGLPGLLQSLNFRGREKPLTIY-GPKGLKKVENAIYSL-GYCKFDFPIEFIEISSG 120 Query: 158 RVLDAGEFTIDCFAVRHRDTDSFAFSFQSAMRRHLLPDRLLALRVPDGPVRRELAEGRQV 217 +L+ E+ I V H T+ A+S + + L ++ + L VP GP+ +L G +V Sbjct: 121 TILETDEYIIKSQEVMHNVTN-LAYSIEEKKKPRFLRNKAIELGVPVGPLFGKLHNGEEV 179 Query: 218 KLDDGRTIDPEQVLGSAEGRKKLVIVGDAETTEGLAELVRDADVLVIEATFLERDAAIAR 277 ++ +G+ I PEQVLG A K+ GD E + E +++ +L+ E+T+ D AR Sbjct: 180 EV-NGKIIKPEQVLGDARKGIKITYSGDTRPCEEMIEFAKNSTLLIHESTYTLEDDDKAR 238 Query: 278 DYGHLTASEAAALAARSGVKQLILTHISGRYPD-EDILAEATRIFPNSRIAIDFDRIAI 335 + H T+ +AA +A S K+L+LTHIS RY D ED+L EA ++F N +A D I I Sbjct: 239 ENFHSTSQDAATIAKCSNTKRLVLTHISTRYTDTEDLLKEAKKVFENVEVADDLMTIEI 297