BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0050.0062.p (491 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_571404.1| amidase [Rhodopseudomonas palustris BisB5] >gi|... 457 e-126 gb|ADL62710.1| amidase [synthetic construct] 451 e-124 ref|YP_004486692.1| Amidase [Delftia sp. Cs1-4] >gi|333743160|gb... 451 e-124 ref|YP_001566322.1| amidase [Delftia acidovorans SPH-1] >gi|1603... 451 e-124 dbj|BAE87102.1| D-amino-acid amidase [Delftia acidovorans] 451 e-124 >ref|YP_571404.1| amidase [Rhodopseudomonas palustris BisB5] gb|ABE41503.1| Amidase [Rhodopseudomonas palustris BisB5] Length = 466 Score = 457 bits (1175), Expect = e-126, Method: Compositional matrix adjust. Identities = 237/463 (51%), Positives = 306/463 (66%), Gaps = 11/463 (2%) Query: 29 QMHYLTLSELSVLLRTKAISPVELVKHMLGRIDALEPRLESYVTITAERALEQAERAERE 88 +HY +++ELS L R K + P E+ + L RI L+PR Y + AERA++QA+R++ E Sbjct: 4 DLHYKSITELSDLYRRKQLKPSEVTQASLDRIAKLDPRFHGYAVVLAERAMQQAKRSDDE 63 Query: 89 IGSGQWRGHLHGVPLAYKDIFYTDFALTTAGTAIHKSFQPTYSATAVRRLEAAGAVSLGK 148 I G WRG LHGVP+ KD+ YT FA T GT IH +F P+++AT V RLE GAVSLGK Sbjct: 64 IARGIWRGPLHGVPIGLKDLCYTTFAPTAGGTKIHATFVPSFNATIVDRLERGGAVSLGK 123 Query: 149 LKTTEQAWTEHHPAVAAPKNPWGAELYAGASSSGSGVATAAGLTFGALGSDTGGSIRFPS 208 LK TE A+T HHP AAP NPW + + G+SSSGSGVAT+AGL +GA+GSDTGGSIRFPS Sbjct: 124 LKMTEGAYTSHHPDDAAPLNPWNVDYWVGSSSSGSGVATSAGLCYGAIGSDTGGSIRFPS 183 Query: 209 AVNGVTGLKPTWGRVSRHGVFELAASLDHVGPMARSAIDCAIMLGAIAGADPADPTAVLN 268 A G+TG+KPTWGRVSR+GVF LA SLDHVGPM RSA D A MLG IAGADP DPT + Sbjct: 184 ATCGLTGIKPTWGRVSRYGVFPLADSLDHVGPMCRSAADAAAMLGVIAGADPNDPTTLQA 243 Query: 269 PIEDYEAAALRSVAGMRIGLPRGYATEGVDPGIVAVWQAAAKVFEDLGAIVEEISFPEWR 328 P+ +Y A V G+RIG+ R YA +G+DP +VA + A + DLGA + E+ P + Sbjct: 244 PVPNYLAQIDDGVRGLRIGIDRSYAEQGIDPQVVAGLREAERTLADLGAEIREVRLPAYE 303 Query: 329 KAASTWPILCSSETALTHAATYPSKSESYGTALSALIDLGRSSTAMDLAAAHQARLEFSS 388 K W +CS ETA H ATYPS+ YG L+ LI+ G + T +++AA H RL FS Sbjct: 304 KLVGHWIPMCSVETAEAHLATYPSRKSEYGPDLAQLIEQGHAMTGVEIAAIHHERLRFSG 363 Query: 389 RLRARFDNLDVILVPATIWPTPTA------AATPDILQKSPLEDLLRFTCVFDSSGSPTL 442 L A F+++D++L P P PT A P++L ++LRFT FD SGSPT+ Sbjct: 364 GLAALFEDIDLLLAPTMPVPIPTLTRMGEYGADPNVLL-----NILRFTAPFDFSGSPTI 418 Query: 443 TLPGGFDGGGTPMAIQLIGAHFDEAAVLQAGAAFQRATDWHSK 485 T+P G P++ QL+G H E + +AG A+Q TDWH+K Sbjct: 419 TMPMGMASDAMPLSFQLVGPHLSEHVLARAGQAYQSITDWHTK 461 >gb|ADL62710.1| amidase [synthetic construct] Length = 467 Score = 451 bits (1161), Expect = e-124, Method: Compositional matrix adjust. Identities = 248/463 (53%), Positives = 322/463 (69%), Gaps = 3/463 (0%) Query: 26 TENQMHYLTLSELSVLLRTKAISPVELVKHMLGRIDALEPRLESYVTITAERALEQAERA 85 ++++H+L L E+ ++++ IS E+ +HML RI+A++ RL SYVT+ A++ALE A RA Sbjct: 3 NDSELHHLELLEVGREIQSRRISSEEVTRHMLARIEAVDARLHSYVTVMAQQALEDARRA 62 Query: 86 EREIGSGQWRGHLHGVPLAYKDIFYTDFALTTAGTAIHKSFQPTYSATAVRRLEAAGAVS 145 + EI G+ RG LHGVPLA KD+ +T TT G +H+ +PT AT VRRL AGAV Sbjct: 63 DAEIAQGRRRGALHGVPLALKDLLWTRGVPTTHGMTLHREHRPTEDATVVRRLREAGAVI 122 Query: 146 LGKLKTTEQAWTEHHPAVAAPKNPWGAELYAGASSSGSGVATAAGLTFGALGSDTGGSIR 205 LGKL+ TE A+ +HHP + AP NPWGA+L+ GASSSGSGVATAAGL FG+LG+DTGGSIR Sbjct: 123 LGKLQQTEGAFADHHPEITAPVNPWGAQLWPGASSSGSGVATAAGLCFGSLGTDTGGSIR 182 Query: 206 FPSAVNGVTGLKPTWGRVSRHGVFELAASLDHVGPMARSAIDCAIMLGAIAGADPADPTA 265 FPSA NG+TGLKPTWGRVSRHG FELAASLDH+GPMARSA D A ML AIAGADP DPTA Sbjct: 183 FPSAANGITGLKPTWGRVSRHGAFELAASLDHIGPMARSAADAAAMLAAIAGADPLDPTA 242 Query: 266 VLNPIEDYEAAALRSVAGMRIGLPRGYATEGVD-PGIVAVWQAAAKVFEDLGAIVEEISF 324 + DY A R +G+R+G+ R +A +GVD P AV QA A V + LGA V+E+ F Sbjct: 243 SQCSVPDYLAMMTRGFSGLRLGMDRQWALDGVDAPSRQAVEQALA-VAQRLGASVQEVRF 301 Query: 325 PEWRKAASTWPILCSSETALTHAATYPSKSESYGTALSALIDLGRSSTAMDLAAAHQARL 384 P+ +A WP LC+ ETA+ H AT+P++ E+YG L+ LIDLG +A D R Sbjct: 302 PDATQAVQDWPALCAVETAVAHGATFPARREAYGPGLAGLIDLGLGLSATDYQRLLLRRA 361 Query: 385 EFSSRLRARFDNLDVILVPATIWPTPTAAATPDILQKSPL-EDLLRFTCVFDSSGSPTLT 443 +F+ R+RA F +D++LVPAT + PT + L +LR+TC FD +GSPT+T Sbjct: 362 DFTGRVRALFAQVDLLLVPATAFAAPTLQRMAHFGSDAELFSGMLRYTCPFDLTGSPTIT 421 Query: 444 LPGGFDGGGTPMAIQLIGAHFDEAAVLQAGAAFQRATDWHSKH 486 LPGG G P+A Q + F E +++AG AFQ+ATDWH +H Sbjct: 422 LPGGRTPEGAPVAFQFVAPDFREDLLVRAGWAFQQATDWHRQH 464 >ref|YP_004486692.1| Amidase [Delftia sp. Cs1-4] gb|AEF88337.1| Amidase [Delftia sp. Cs1-4] Length = 466 Score = 451 bits (1161), Expect = e-124, Method: Compositional matrix adjust. Identities = 248/463 (53%), Positives = 322/463 (69%), Gaps = 3/463 (0%) Query: 26 TENQMHYLTLSELSVLLRTKAISPVELVKHMLGRIDALEPRLESYVTITAERALEQAERA 85 ++++H+L L E+ ++++ IS E+ +HML RI+A++ RL SYVT+ A++ALE A RA Sbjct: 2 NDSELHHLELLEVGREIQSRRISSEEVTRHMLARIEAVDARLHSYVTVMAQQALEDARRA 61 Query: 86 EREIGSGQWRGHLHGVPLAYKDIFYTDFALTTAGTAIHKSFQPTYSATAVRRLEAAGAVS 145 + EI G+ RG LHGVPLA KD+ +T TT G +H+ +PT AT VRRL AGAV Sbjct: 62 DAEIAQGRRRGALHGVPLALKDLLWTRGVPTTHGMTLHRDHRPTEDATVVRRLREAGAVI 121 Query: 146 LGKLKTTEQAWTEHHPAVAAPKNPWGAELYAGASSSGSGVATAAGLTFGALGSDTGGSIR 205 LGKL+ TE A+ +HHP + AP NPWGA+L+ GASSSGSGVATAAGL FG+LG+DTGGSIR Sbjct: 122 LGKLQQTEGAFADHHPEITAPVNPWGAQLWPGASSSGSGVATAAGLCFGSLGTDTGGSIR 181 Query: 206 FPSAVNGVTGLKPTWGRVSRHGVFELAASLDHVGPMARSAIDCAIMLGAIAGADPADPTA 265 FPSA NG+TGLKPTWGRVSRHG FELAASLDH+GPMARSA D A ML AIAGADP DPTA Sbjct: 182 FPSAANGITGLKPTWGRVSRHGAFELAASLDHIGPMARSAADAAAMLAAIAGADPLDPTA 241 Query: 266 VLNPIEDYEAAALRSVAGMRIGLPRGYATEGVD-PGIVAVWQAAAKVFEDLGAIVEEISF 324 + DY A R +G+R+G+ R +A +GVD P AV QA A V + LGA V+E+ F Sbjct: 242 SQCSVPDYLAMMTRGFSGLRLGMDRQWALDGVDAPSRQAVEQALA-VAQRLGASVQEVRF 300 Query: 325 PEWRKAASTWPILCSSETALTHAATYPSKSESYGTALSALIDLGRSSTAMDLAAAHQARL 384 P+ +A WP LC+ ETA+ H AT+P++ E+YG L+ LIDLG +A D R Sbjct: 301 PDATQAVEDWPALCAVETAVAHGATFPARREAYGPGLAGLIDLGLGLSATDYQRLLLRRA 360 Query: 385 EFSSRLRARFDNLDVILVPATIWPTPTAAATPDILQKSPL-EDLLRFTCVFDSSGSPTLT 443 +F+ R+RA F +D++LVPAT + PT + L +LR+TC FD +GSPT+T Sbjct: 361 DFTGRVRALFAQVDLLLVPATAFAAPTLQRMAHFGSDAELFSGMLRYTCPFDLTGSPTIT 420 Query: 444 LPGGFDGGGTPMAIQLIGAHFDEAAVLQAGAAFQRATDWHSKH 486 LPGG G P+A Q + F E +++AG AFQ+ATDWH +H Sbjct: 421 LPGGRTPEGAPVAFQFVAPDFREDLLVRAGWAFQQATDWHRQH 463 >ref|YP_001566322.1| amidase [Delftia acidovorans SPH-1] gb|ABX37937.1| Amidase [Delftia acidovorans SPH-1] Length = 466 Score = 451 bits (1161), Expect = e-124, Method: Compositional matrix adjust. Identities = 248/463 (53%), Positives = 322/463 (69%), Gaps = 3/463 (0%) Query: 26 TENQMHYLTLSELSVLLRTKAISPVELVKHMLGRIDALEPRLESYVTITAERALEQAERA 85 ++++H+L L E+ ++++ IS E+ +HML RI+A++ RL SYVT+ A++ALE A RA Sbjct: 2 NDSELHHLELLEVGREIQSRRISSEEVTRHMLARIEAVDARLHSYVTVMAQQALEDARRA 61 Query: 86 EREIGSGQWRGHLHGVPLAYKDIFYTDFALTTAGTAIHKSFQPTYSATAVRRLEAAGAVS 145 + EI G+ RG LHGVPLA KD+ +T TT G +H+ +PT AT VRRL AGAV Sbjct: 62 DAEIAQGRRRGALHGVPLALKDLLWTRGVPTTHGMTLHREHRPTEDATVVRRLREAGAVI 121 Query: 146 LGKLKTTEQAWTEHHPAVAAPKNPWGAELYAGASSSGSGVATAAGLTFGALGSDTGGSIR 205 LGKL+ TE A+ +HHP + AP NPWGA+L+ GASSSGSGVATAAGL FG+LG+DTGGSIR Sbjct: 122 LGKLQQTEGAFADHHPEITAPVNPWGAQLWPGASSSGSGVATAAGLCFGSLGTDTGGSIR 181 Query: 206 FPSAVNGVTGLKPTWGRVSRHGVFELAASLDHVGPMARSAIDCAIMLGAIAGADPADPTA 265 FPSA NG+TGLKPTWGRVSRHG FELAASLDH+GPMARSA D A ML AIAGADP DPTA Sbjct: 182 FPSAANGITGLKPTWGRVSRHGAFELAASLDHIGPMARSAADAAAMLAAIAGADPLDPTA 241 Query: 266 VLNPIEDYEAAALRSVAGMRIGLPRGYATEGVD-PGIVAVWQAAAKVFEDLGAIVEEISF 324 + DY A R +G+R+G+ R +A +GVD P AV QA A V + LGA V+E+ F Sbjct: 242 SQCSVPDYLAMMTRGFSGLRLGMDRQWALDGVDAPSRQAVEQALA-VAQRLGASVQEVRF 300 Query: 325 PEWRKAASTWPILCSSETALTHAATYPSKSESYGTALSALIDLGRSSTAMDLAAAHQARL 384 P+ +A WP LC+ ETA+ H AT+P++ E+YG L+ LIDLG +A D R Sbjct: 301 PDATQAVQDWPALCAVETAVAHGATFPARREAYGPGLAGLIDLGLGLSATDYQRLLLRRA 360 Query: 385 EFSSRLRARFDNLDVILVPATIWPTPTAAATPDILQKSPL-EDLLRFTCVFDSSGSPTLT 443 +F+ R+RA F +D++LVPAT + PT + L +LR+TC FD +GSPT+T Sbjct: 361 DFTGRVRALFAQVDLLLVPATAFAAPTLQRMAHFGSDAELFSGMLRYTCPFDLTGSPTIT 420 Query: 444 LPGGFDGGGTPMAIQLIGAHFDEAAVLQAGAAFQRATDWHSKH 486 LPGG G P+A Q + F E +++AG AFQ+ATDWH +H Sbjct: 421 LPGGRTPEGAPVAFQFVAPDFREDLLVRAGWAFQQATDWHRQH 463 >dbj|BAE87102.1| D-amino-acid amidase [Delftia acidovorans] Length = 466 Score = 451 bits (1159), Expect = e-124, Method: Compositional matrix adjust. Identities = 247/463 (53%), Positives = 322/463 (69%), Gaps = 3/463 (0%) Query: 26 TENQMHYLTLSELSVLLRTKAISPVELVKHMLGRIDALEPRLESYVTITAERALEQAERA 85 ++++H+L L E+ ++++ IS E+ +HML RI+A++ RL SYVT+ A++A+E A RA Sbjct: 2 NDSELHHLELLEVGREIQSRRISSEEVTRHMLARIEAVDARLHSYVTVMAQQAMEDARRA 61 Query: 86 EREIGSGQWRGHLHGVPLAYKDIFYTDFALTTAGTAIHKSFQPTYSATAVRRLEAAGAVS 145 + EI G+ RG LHGVPLA KD+ +T TT G +H+ +PT AT VRRL AGAV Sbjct: 62 DAEIAQGRRRGALHGVPLALKDLLWTRGVPTTHGMTLHRDHRPTEDATVVRRLREAGAVI 121 Query: 146 LGKLKTTEQAWTEHHPAVAAPKNPWGAELYAGASSSGSGVATAAGLTFGALGSDTGGSIR 205 LGKL+ TE A+ +HHP + AP NPWGA+L+ GASSSGSGVATAAGL FG+LG+DTGGSIR Sbjct: 122 LGKLQQTEGAFADHHPEITAPVNPWGAQLWPGASSSGSGVATAAGLCFGSLGTDTGGSIR 181 Query: 206 FPSAVNGVTGLKPTWGRVSRHGVFELAASLDHVGPMARSAIDCAIMLGAIAGADPADPTA 265 FPSA NG+TGLKPTWGRVSRHG FELAASLDH+GPMARSA D A ML AIAGADP DPTA Sbjct: 182 FPSAANGITGLKPTWGRVSRHGAFELAASLDHIGPMARSAADAAAMLAAIAGADPLDPTA 241 Query: 266 VLNPIEDYEAAALRSVAGMRIGLPRGYATEGVD-PGIVAVWQAAAKVFEDLGAIVEEISF 324 + DY A R +G+R+G+ R +A +GVD P AV QA A V + LGA V+E+ F Sbjct: 242 SQCSVPDYLALMTRGFSGLRLGMDRQWALDGVDAPSRQAVEQALA-VAQRLGASVQEVRF 300 Query: 325 PEWRKAASTWPILCSSETALTHAATYPSKSESYGTALSALIDLGRSSTAMDLAAAHQARL 384 P+ +A WP LC+ ETA+ H AT+P++ E+YG L+ LIDLG +A D R Sbjct: 301 PDATQAVEDWPALCAVETAVAHGATFPARREAYGPGLAGLIDLGLGLSATDYQRLLLRRA 360 Query: 385 EFSSRLRARFDNLDVILVPATIWPTPTAAATPDILQKSPL-EDLLRFTCVFDSSGSPTLT 443 +F+ R+RA F +D++LVPAT + PT + L +LR+TC FD +GSPT+T Sbjct: 361 DFTGRVRALFAQVDLLLVPATAFAAPTLQRMAHFGSDAELFSGMLRYTCPFDLTGSPTIT 420 Query: 444 LPGGFDGGGTPMAIQLIGAHFDEAAVLQAGAAFQRATDWHSKH 486 LPGG G P+A Q + F E +++AG AFQ+ATDWH +H Sbjct: 421 LPGGRTPEGAPVAFQFVAPDFREDLLVRAGWAFQQATDWHRRH 463