BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0050.0054.p (295 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_07676740.1| sterol desaturase [Ralstonia sp. 5_7_47FAA] >... 526 e-147 ref|YP_002981430.1| fatty acid hydroxylase [Ralstonia pickettii ... 525 e-147 ref|YP_001899003.1| fatty acid hydroxylase [Ralstonia pickettii ... 524 e-147 ref|YP_296977.1| putative sterol desaturase [Ralstonia eutropha ... 447 e-124 ref|ZP_01916737.1| putative Sterol desaturase [Limnobacter sp. M... 397 e-109 >ref|ZP_07676740.1| sterol desaturase [Ralstonia sp. 5_7_47FAA] gb|EFP64743.1| sterol desaturase [Ralstonia sp. 5_7_47FAA] Length = 300 Score = 526 bits (1356), Expect = e-147, Method: Compositional matrix adjust. Identities = 246/294 (83%), Positives = 268/294 (91%) Query: 1 MSSQLQYLLIGACILGFAATELITRRYQATVRDHATANDTWLEVLMFVSLIAITQPIALL 60 MSSQ+++ LI ACILGFAA E +TRRYQA VR ATAND WLEVLMF+SL+A+TQPIALL Sbjct: 1 MSSQMEHALIVACILGFAAMEFVTRRYQANVRGRATANDAWLEVLMFISLVAVTQPIALL 60 Query: 61 GSNALCNWLIPAQHNAWANLPWWAMAGLLLIGDDLTQYLWHRASHSPLLWPLHRAHHSAA 120 GSNALCNWLIPAQHNAWA+LPWWAM GLLLIGDDLTQYLWHRASH+PLLWPLHRAHHSA+ Sbjct: 61 GSNALCNWLIPAQHNAWADLPWWAMTGLLLIGDDLTQYLWHRASHTPLLWPLHRAHHSAS 120 Query: 121 YMSVRITYRNNFFYYLMMPGLWIGGVAAYLGFGPVYAAYLVIKVTVILGAHCAWAWDAPL 180 YMSVR+TYRNNFFYYLMMPGLWIGGVA YLGFGPVYAAYLV+K+TVI+GAHCAW WDAPL Sbjct: 121 YMSVRVTYRNNFFYYLMMPGLWIGGVAVYLGFGPVYAAYLVVKLTVIVGAHCAWPWDAPL 180 Query: 181 YRIRALHPLMWVLERTISTPATHQAHHALTNEDGIGHYKGNFGNLLFFWDVLFGTAHITR 240 YR+RAL PLMWV+ERTISTPATH AHHALTN DGIGHYKGNFGNLLFFWDVLFGTAHITR Sbjct: 181 YRVRALRPLMWVIERTISTPATHWAHHALTNADGIGHYKGNFGNLLFFWDVLFGTAHITR 240 Query: 241 KYPAKVGLQDDILFGPERWTTQMFYPLVHSRREHSALRPGGYGFTEADLQTEAK 294 KYPA++GLQDD+LFG E WT QMFYPLV S+REHSALRPGG TE ++ A Sbjct: 241 KYPAQIGLQDDVLFGAEPWTAQMFYPLVQSKREHSALRPGGAAVTELEMNQAAS 294 >ref|YP_002981430.1| fatty acid hydroxylase [Ralstonia pickettii 12D] gb|ACS62758.1| fatty acid hydroxylase [Ralstonia pickettii 12D] Length = 300 Score = 525 bits (1352), Expect = e-147, Method: Compositional matrix adjust. Identities = 245/294 (83%), Positives = 267/294 (90%) Query: 1 MSSQLQYLLIGACILGFAATELITRRYQATVRDHATANDTWLEVLMFVSLIAITQPIALL 60 MSSQ+++ LI ACILGFAA E +TRRYQA VR ATAND WLEVLMF+SL+A+TQPIALL Sbjct: 1 MSSQMEHALIVACILGFAAMEFVTRRYQANVRGRATANDAWLEVLMFISLVAVTQPIALL 60 Query: 61 GSNALCNWLIPAQHNAWANLPWWAMAGLLLIGDDLTQYLWHRASHSPLLWPLHRAHHSAA 120 GSNALC WLIPAQHNAWA+LPWWAM GLLLIGDDLTQYLWHRASH+PLLWPLHRAHHSA+ Sbjct: 61 GSNALCKWLIPAQHNAWADLPWWAMTGLLLIGDDLTQYLWHRASHTPLLWPLHRAHHSAS 120 Query: 121 YMSVRITYRNNFFYYLMMPGLWIGGVAAYLGFGPVYAAYLVIKVTVILGAHCAWAWDAPL 180 YMSVR+TYRNNFFYYLMMPGLWIGGVA YLGFGPVYAAYLV+K+TVI+GAHCAW WDAPL Sbjct: 121 YMSVRVTYRNNFFYYLMMPGLWIGGVAVYLGFGPVYAAYLVVKLTVIVGAHCAWPWDAPL 180 Query: 181 YRIRALHPLMWVLERTISTPATHQAHHALTNEDGIGHYKGNFGNLLFFWDVLFGTAHITR 240 YR+RAL PLMWV+ERTISTPATH AHHALTN DGIGHYKGNFGNLLFFWDVLFGTAHITR Sbjct: 181 YRVRALRPLMWVIERTISTPATHWAHHALTNADGIGHYKGNFGNLLFFWDVLFGTAHITR 240 Query: 241 KYPAKVGLQDDILFGPERWTTQMFYPLVHSRREHSALRPGGYGFTEADLQTEAK 294 KYPA++GLQDD+LFG E WT QMFYPLV S+REHSALRPGG TE ++ A Sbjct: 241 KYPAQIGLQDDVLFGAEPWTAQMFYPLVQSKREHSALRPGGAAVTEMEMNQAAS 294 >ref|YP_001899003.1| fatty acid hydroxylase [Ralstonia pickettii 12J] gb|ACD26571.1| fatty acid hydroxylase [Ralstonia pickettii 12J] Length = 300 Score = 524 bits (1349), Expect = e-147, Method: Compositional matrix adjust. Identities = 247/294 (84%), Positives = 266/294 (90%) Query: 1 MSSQLQYLLIGACILGFAATELITRRYQATVRDHATANDTWLEVLMFVSLIAITQPIALL 60 MSS +++ LI ACILGFAA E +TRRYQA VR ATAND WLEVLMF+SL+A+TQPIALL Sbjct: 1 MSSHMEHALIVACILGFAAMEFVTRRYQANVRGRATANDAWLEVLMFISLVAVTQPIALL 60 Query: 61 GSNALCNWLIPAQHNAWANLPWWAMAGLLLIGDDLTQYLWHRASHSPLLWPLHRAHHSAA 120 GSNALCNWLIPAQHNAWA+LPWWAM GLLLIGDDLTQYLWHRASH+PLLWPLHRAHHSA+ Sbjct: 61 GSNALCNWLIPAQHNAWADLPWWAMTGLLLIGDDLTQYLWHRASHTPLLWPLHRAHHSAS 120 Query: 121 YMSVRITYRNNFFYYLMMPGLWIGGVAAYLGFGPVYAAYLVIKVTVILGAHCAWAWDAPL 180 YMSVRITYRNNFFYYLMMPGLWIGGVA YLGFGPVYAAYLV+K+TVI+GAHCAW WDAPL Sbjct: 121 YMSVRITYRNNFFYYLMMPGLWIGGVAVYLGFGPVYAAYLVVKLTVIVGAHCAWPWDAPL 180 Query: 181 YRIRALHPLMWVLERTISTPATHQAHHALTNEDGIGHYKGNFGNLLFFWDVLFGTAHITR 240 YRIRAL PLMWV+ERTISTPATH AHHALTN DGIGHYKGNFGNLLFFWDVLFGTAHITR Sbjct: 181 YRIRALRPLMWVIERTISTPATHWAHHALTNADGIGHYKGNFGNLLFFWDVLFGTAHITR 240 Query: 241 KYPAKVGLQDDILFGPERWTTQMFYPLVHSRREHSALRPGGYGFTEADLQTEAK 294 KYPA++GLQDD+LFG E WT QMFYPLV SRRE SALRPGG TE ++ A Sbjct: 241 KYPAQIGLQDDVLFGAEPWTAQMFYPLVQSRREQSALRPGGAAITEMEVDQAAS 294 >ref|YP_296977.1| putative sterol desaturase [Ralstonia eutropha JMP134] gb|AAZ62133.1| putative Sterol desaturase [Ralstonia eutropha JMP134] Length = 297 Score = 447 bits (1150), Expect = e-124, Method: Compositional matrix adjust. Identities = 212/292 (72%), Positives = 241/292 (82%), Gaps = 2/292 (0%) Query: 1 MSSQLQYLLIGACILGFAATELITRRYQATVRDHATANDTWLEVLMFVSLIAITQPIALL 60 M+ QLQ LLIG+ I+GFA E +RRYQ +VR A ANDT LEVLMFVSLIA+TQP+ALL Sbjct: 1 MNLQLQNLLIGSIIVGFAVMEFASRRYQQSVR--ADANDTKLEVLMFVSLIAVTQPLALL 58 Query: 61 GSNALCNWLIPAQHNAWANLPWWAMAGLLLIGDDLTQYLWHRASHSPLLWPLHRAHHSAA 120 G+N LC WLIP H AW++L WW+M +LL+ DD+TQY WHRASH+P LWPLHRAHHSA+ Sbjct: 59 GANWLCGWLIPEMHGAWSHLSWWSMVAILLVADDMTQYWWHRASHTPWLWPLHRAHHSAS 118 Query: 121 YMSVRITYRNNFFYYLMMPGLWIGGVAAYLGFGPVYAAYLVIKVTVILGAHCAWAWDAPL 180 YMS+R+TYRNNFFYYLMMPGLWI GV YLGFG VY Y+V+K+ VILGAHCAW WD PL Sbjct: 119 YMSIRVTYRNNFFYYLMMPGLWIAGVLLYLGFGKVYGVYIVVKMLVILGAHCAWRWDEPL 178 Query: 181 YRIRALHPLMWVLERTISTPATHQAHHALTNEDGIGHYKGNFGNLLFFWDVLFGTAHITR 240 YR+RAL PLMWV+ERTISTPATH AHHALTN DGIGHYKGNFGNLLFFWDVLFGTAHITR Sbjct: 179 YRMRALRPLMWVVERTISTPATHWAHHALTNADGIGHYKGNFGNLLFFWDVLFGTAHITR 238 Query: 241 KYPAKVGLQDDILFGPERWTTQMFYPLVHSRREHSALRPGGYGFTEADLQTE 292 KYPAKVGL+DD FG E W QMFYPL S+R+H+ALR GG F + D + Sbjct: 239 KYPAKVGLEDDRRFGAEHWAAQMFYPLRQSQRQHTALRFGGEAFGDQDTAAD 290 >ref|ZP_01916737.1| putative Sterol desaturase [Limnobacter sp. MED105] gb|EDM82037.1| putative Sterol desaturase [Limnobacter sp. MED105] Length = 291 Score = 397 bits (1021), Expect = e-109, Method: Compositional matrix adjust. Identities = 192/285 (67%), Positives = 224/285 (78%), Gaps = 3/285 (1%) Query: 3 SQLQYLLIGACILGFAATELITRRYQATVRDHATANDTWLEVLMFVSLIAITQPIALLGS 62 S+ Q +LI I GFA E +TRRYQ TV +ATANDT LE+ MF+SL+A+TQP+ +L + Sbjct: 4 SEFQNVLILIAIAGFALMEFVTRRYQTTV--NATANDTKLELFMFISLVALTQPLVILAT 61 Query: 63 NALCNWLIPAQHNAWANLPWWAMAGLLLIGDDLTQYLWHRASHSPLLWPLHRAHHSAAYM 122 + WA+ PWWAM +LL+GDDLTQYLWHRASHSPLLWPLHRAHHSA YM Sbjct: 62 QNMGEAWFSQWQGIWADWPWWAMFAVLLLGDDLTQYLWHRASHSPLLWPLHRAHHSAQYM 121 Query: 123 SVRITYRNNFFYYLMMPGLWIGGVAAYLGFGP-VYAAYLVIKVTVILGAHCAWAWDAPLY 181 SVR+T+RNNFFYYLMMPGLWI G +LGFG VYAAYL +K+T+ILGAHC+W WD PLY Sbjct: 122 SVRMTFRNNFFYYLMMPGLWIAGALLFLGFGGWVYAAYLTLKLTIILGAHCSWRWDEPLY 181 Query: 182 RIRALHPLMWVLERTISTPATHQAHHALTNEDGIGHYKGNFGNLLFFWDVLFGTAHITRK 241 +IRALHPLMW+LERTISTPATH AHHALTN+DGIGHY GNFGN+LF WD++FGTAHITRK Sbjct: 182 KIRALHPLMWLLERTISTPATHWAHHALTNKDGIGHYTGNFGNMLFLWDIIFGTAHITRK 241 Query: 242 YPAKVGLQDDILFGPERWTTQMFYPLVHSRREHSALRPGGYGFTE 286 YP +GL DD + G E W QMFYPL HS+R SALR GG + E Sbjct: 242 YPDHIGLMDDRIHGQEHWAHQMFYPLAHSKRADSALRFGGTIYDE 286