BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0048.0002.p (375 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_487665.1| mandelate racemase/muconate lactonizing protein... 509 e-142 ref|NP_772989.1| mandelate racemase [Bradyrhizobium japonicum US... 484 e-135 ref|YP_002947379.1| Mandelate racemase/muconate lactonizing prot... 393 e-107 ref|ZP_02191360.1| putative mandelate racemase/muconate lactoniz... 392 e-107 gb|ADI19035.1| l-alanine-dl-glutamate epimerase and related enzy... 383 e-104 >ref|YP_487665.1| mandelate racemase/muconate lactonizing protein [Rhodopseudomonas palustris HaA2] gb|ABD08754.1| Mandelate racemase/muconate lactonizing enzyme [Rhodopseudomonas palustris HaA2] Length = 375 Score = 509 bits (1310), Expect = e-142, Method: Compositional matrix adjust. Identities = 250/374 (66%), Positives = 285/374 (76%) Query: 1 MTSPPFTIRSVQAFGFRYPLTTPVITSFGRMGDRPAVFVRVEDTDGYAGWGEVWCNFPSP 60 MT+ FTIRS++A FRYPL+ PV+TSFGRM RPAVF+RV D DG +GWGE W NFP+ Sbjct: 1 MTAQDFTIRSIEAMCFRYPLSLPVVTSFGRMTTRPAVFIRVTDEDGISGWGEAWANFPAT 60 Query: 61 GAEHRVRLVNEVLAPALVGFRASEPDAAFERLTRGTSVLALQSGEAGPFAQSIAGIDLAV 120 GAEHR R +NEVLAP G R + P FE LT+ T+VLALQSGE GPFAQ+IAGIDLA+ Sbjct: 61 GAEHRARTINEVLAPLAAGARIAHPSEFFEALTQRTAVLALQSGEQGPFAQAIAGIDLAL 120 Query: 121 WDLHARRRNVALWKLLGGSRKRIKVYASGINPVGSERAAEAALGRGYRALKLKIGFDPAD 180 WDL ARRR LW+LLGG+ IKVYASGINP G+ AE AL RG+RALKLKIGF Sbjct: 121 WDLFARRRATPLWRLLGGANATIKVYASGINPTGTREMAETALARGHRALKLKIGFGAEI 180 Query: 181 DRANLAALRRLVGDGMLAADVNQGWTIAQALERAPQLEPFGLAWLEEPIRADRPRQEWKQ 240 D NLAALR L GDG LAAD NQ WT+ QA E AP L + LAWLEEPIRADRP EW+ Sbjct: 181 DHPNLAALRALAGDGTLAADANQAWTLQQACEAAPHLRDYNLAWLEEPIRADRPWPEWQA 240 Query: 241 LHETIRFPLAAGENIASHAGFAEVLSEPVLGIVQPDIAKWGGLSACAGIARDILASGKMF 300 L PLAAGEN AS F + LS+ LG++QPDIAKWGGLSACA IARDI+A+GK F Sbjct: 241 LRRAATMPLAAGENFASRESFQQALSDDTLGVIQPDIAKWGGLSACAPIARDIVAAGKRF 300 Query: 301 CPHYLGAGIGLLASAHLLAGVGGEGLLEVDSNENPLRDQFCGPVLDVSDGMIELGDGAGL 360 CPHYLG GIGLLASAHLLAG+GG+GLLEVD+N+NPLR+ FCGPV +SDG I LGD GL Sbjct: 301 CPHYLGGGIGLLASAHLLAGIGGDGLLEVDANDNPLREAFCGPVAAISDGAITLGDAPGL 360 Query: 361 GITPDLASIERYRT 374 G+ PDL I +YRT Sbjct: 361 GVEPDLVGIAQYRT 374 >ref|NP_772989.1| mandelate racemase [Bradyrhizobium japonicum USDA 110] dbj|BAC51614.1| bll6349 [Bradyrhizobium japonicum USDA 110] Length = 374 Score = 484 bits (1246), Expect = e-135, Method: Compositional matrix adjust. Identities = 244/369 (66%), Positives = 288/369 (78%) Query: 6 FTIRSVQAFGFRYPLTTPVITSFGRMGDRPAVFVRVEDTDGYAGWGEVWCNFPSPGAEHR 65 FT+RS++ FRYPL TPV+TSFG+M +RPAVFVRV D DG GWGE W NFP+PGAEHR Sbjct: 5 FTVRSIEGVCFRYPLATPVVTSFGKMLNRPAVFVRVVDEDGVEGWGEAWSNFPAPGAEHR 64 Query: 66 VRLVNEVLAPALVGFRASEPDAAFERLTRGTSVLALQSGEAGPFAQSIAGIDLAVWDLHA 125 RL+NEVLAP LVG + P AFE L++GT VLALQ GE GPFAQ+I+GIDLA+WDL A Sbjct: 65 ARLINEVLAPGLVGRKLENPAQAFEVLSKGTEVLALQCGEPGPFAQAISGIDLALWDLFA 124 Query: 126 RRRNVALWKLLGGSRKRIKVYASGINPVGSERAAEAALGRGYRALKLKIGFDPADDRANL 185 RRRN+ LW+LLGG +IKVYASGINP G+ + AEAAL RG+RALKLK+GF D ANL Sbjct: 125 RRRNLPLWRLLGGQSSKIKVYASGINPGGAAQTAEAALKRGHRALKLKVGFGAETDIANL 184 Query: 186 AALRRLVGDGMLAADVNQGWTIAQALERAPQLEPFGLAWLEEPIRADRPRQEWKQLHETI 245 +AL +VG GMLAAD NQGW++ QALE P+L F L WLEEPIRADRPR+EW++L Sbjct: 185 SALLTIVGAGMLAADANQGWSVDQALEMLPRLSEFNLRWLEEPIRADRPREEWRKLRANA 244 Query: 246 RFPLAAGENIASHAGFAEVLSEPVLGIVQPDIAKWGGLSACAGIARDILASGKMFCPHYL 305 + P+AAGENI+S F L + VLG++QPDIAKWGGL+ C +AR IL GK FCPHYL Sbjct: 245 KMPIAAGENISSVEDFEAALGDDVLGVIQPDIAKWGGLTVCVELARQILRVGKTFCPHYL 304 Query: 306 GAGIGLLASAHLLAGVGGEGLLEVDSNENPLRDQFCGPVLDVSDGMIELGDGAGLGITPD 365 G GIGLLASAHLLA VG +G LEVD+N+NPLRD FCGPV DV +G IEL GLGI PD Sbjct: 305 GGGIGLLASAHLLAAVGRDGWLEVDANDNPLRDLFCGPVADVREGTIELNQNPGLGIVPD 364 Query: 366 LASIERYRT 374 L++IERYR+ Sbjct: 365 LSAIERYRS 373 >ref|YP_002947379.1| Mandelate racemase/muconate lactonizing protein [Variovorax paradoxus S110] gb|ACS22113.1| Mandelate racemase/muconate lactonizing protein [Variovorax paradoxus S110] Length = 381 Score = 393 bits (1010), Expect = e-107, Method: Compositional matrix adjust. Identities = 204/372 (54%), Positives = 251/372 (67%), Gaps = 1/372 (0%) Query: 5 PFTIRSVQAFGFRYPLTTPVITSFGRMGDRPAVFVRVEDTDGYAGWGEVWCNFPSPGAEH 64 P + ++AF FR P+ TPV TSFG M DRPAVFVRVED DG GWGEVWCNFPS GAEH Sbjct: 8 PVHVARIEAFVFRCPIATPVRTSFGTMHDRPAVFVRVEDRDGATGWGEVWCNFPSCGAEH 67 Query: 65 RVRLVNEVLAPALVGFRASEPDAAFERLTRGTSVLALQSGEAGPFAQSIAGIDLAVWDLH 124 R +LV+ V+AP L P AF+ LT+ T+VLA+QSGE GP AQ IAGIDLA+WDL Sbjct: 68 RAKLVDTVMAPLLASRAFDGPAEAFDWLTQRTAVLAIQSGEPGPMAQVIAGIDLALWDLC 127 Query: 125 ARRRNVALWKLLGGSRKRIKVYASGINPVGSERAAEAALGRGYRALKLKIGFDPADDRAN 184 ARR LW+ LGG+ + VYASGINP + A A L GYRA KLKIGF D AN Sbjct: 128 ARRAGQPLWRYLGGAHDGVGVYASGINPDRPQDLAAARLAEGYRAFKLKIGFGRERDLAN 187 Query: 185 LAALRRLVGDGM-LAADVNQGWTIAQALERAPQLEPFGLAWLEEPIRADRPRQEWKQLHE 243 LAA+R ++G G+ L D NQ W++ +A APQLE F L WLEEP+RADRP EW+QL + Sbjct: 188 LAAMREVLGPGLPLMTDANQAWSLDEARAMAPQLEAFNLGWLEEPLRADRPWHEWQQLSQ 247 Query: 244 TIRFPLAAGENIASHAGFAEVLSEPVLGIVQPDIAKWGGLSACAGIARDILASGKMFCPH 303 PLAAGENIA A F +++ VL ++QPD AKWGG+S C + A+G +CPH Sbjct: 248 ATGIPLAAGENIAGPAAFDAAIAQGVLSVLQPDSAKWGGISGCRPVIARGQAAGLRYCPH 307 Query: 304 YLGAGIGLLASAHLLAGVGGEGLLEVDSNENPLRDQFCGPVLDVSDGMIELGDGAGLGIT 363 YLG GIGLL SAHLLA GG G+LE+D+N NPLR + V++G LG+ G+G+ Sbjct: 308 YLGGGIGLLHSAHLLAAAGGGGILEIDANPNPLRTALAPALSRVTEGRCSLGEQPGIGVA 367 Query: 364 PDLASIERYRTA 375 PDL ++ + A Sbjct: 368 PDLNAVAQLAAA 379 >ref|ZP_02191360.1| putative mandelate racemase/muconate lactonizing enzyme [alpha proteobacterium BAL199] gb|EDP61884.1| putative mandelate racemase/muconate lactonizing enzyme [alpha proteobacterium BAL199] Length = 376 Score = 392 bits (1008), Expect = e-107, Method: Compositional matrix adjust. Identities = 214/371 (57%), Positives = 251/371 (67%), Gaps = 1/371 (0%) Query: 3 SPPFTIRSVQAFGFRYPLTTPVITSFGRMGDRPAVFVRVEDTDGYAGWGEVWCNFPSPGA 62 +P IRSV A FRYPL T V TSFG M DRP + VRV D G +GWGEVWCNFP GA Sbjct: 2 NPELAIRSVDAHVFRYPLETVVQTSFGVMRDRPMLLVRVTDAGGASGWGEVWCNFPGVGA 61 Query: 63 EHRVRLVNEVLAPALVGFRASEPDAAFERLTRGTSVLALQSGEAGPFAQSIAGIDLAVWD 122 EHR RL++ VLAP LVG + AAF LT T VLALQ E GPFAQ+IAG+D A+WD Sbjct: 62 EHRARLIDSVLAPLLVGRTFEDAAAAFRELTAATEVLALQCAEPGPFAQAIAGLDTALWD 121 Query: 123 LHARRRNVALWKLLGGSRKRIKVYASGINPVGSERAAEAALGRGYRALKLKIGFDPADDR 182 L R LW+LLGG+ VYASG+NP E A GYRA KLKIGF D Sbjct: 122 LSGRLAGAPLWRLLGGTEPSFGVYASGLNPQAPEVLAARRHAEGYRAFKLKIGFGAERDL 181 Query: 183 ANLAALRRLVG-DGMLAADVNQGWTIAQALERAPQLEPFGLAWLEEPIRADRPRQEWKQL 241 ANLAALR +G D L D NQGW++ AL+ AP+LE FGL WLEEP+RADRP EW++L Sbjct: 182 ANLAALRETLGPDVRLMVDANQGWSLDAALDMAPRLEAFGLDWLEEPLRADRPWPEWRRL 241 Query: 242 HETIRFPLAAGENIASHAGFAEVLSEPVLGIVQPDIAKWGGLSACAGIARDILASGKMFC 301 ET PLA GEN+ A F L+E VL +VQPD+AKWGGLSAC +A+ I A+G+ +C Sbjct: 242 KETTSIPLAGGENLMGDAAFDSALAEGVLAVVQPDLAKWGGLSACLPLAKRIRAAGRRYC 301 Query: 302 PHYLGAGIGLLASAHLLAGVGGEGLLEVDSNENPLRDQFCGPVLDVSDGMIELGDGAGLG 361 PHYLG GIGLLASAHLLA VGG+G+LE+D N NPLR+ CGP+ V G L AGLG Sbjct: 302 PHYLGGGIGLLASAHLLAAVGGDGILEIDGNPNPLRELLCGPLAAVEGGRAALTGAAGLG 361 Query: 362 ITPDLASIERY 372 + PDL ++ Y Sbjct: 362 VEPDLKALAPY 372 >gb|ADI19035.1| l-alanine-dl-glutamate epimerase and related enzymes of enolase superfamily [uncultured alpha proteobacterium HF0070_05I22] Length = 372 Score = 383 bits (984), Expect = e-104, Method: Compositional matrix adjust. Identities = 185/368 (50%), Positives = 245/368 (66%), Gaps = 1/368 (0%) Query: 8 IRSVQAFGFRYPLTTPVITSFGRMGDRPAVFVRVEDTDGYAGWGEVWCNFPSPGAEHRVR 67 I++V+ + +RYPL PV TSFG M DRP V V++ D D +G+GE+WCN+P+ GAEHR + Sbjct: 4 IKAVETYVYRYPLEAPVQTSFGTMNDRPMVIVKLVDQDNVSGFGEIWCNYPAVGAEHRAK 63 Query: 68 LVNEVLAPALVGFRASEPDAAFERLTRGTSVLALQSGEAGPFAQSIAGIDLAVWDLHARR 127 +V VL+ + +P+ AFE LT T VL LQ+GE GP AQ IAG+D+A+ DL ARR Sbjct: 64 IVKSVLSNLICSQSFEKPEDAFELLTSKTWVLCLQTGEYGPIAQCIAGVDIAMHDLCARR 123 Query: 128 RNVALWKLLGGSRKRIKVYASGINPVGSERAAEAALGRGYRALKLKIGFDPADDRANLAA 187 + LWK LGG+R ++ YASGI+P + + AEAAL G+ +LKLKIGFD D N+ Sbjct: 124 LSQPLWKYLGGTRNKVSTYASGISPATARKTAEAALAAGHDSLKLKIGFDKDTDLKNIKD 183 Query: 188 LRRLVG-DGMLAADVNQGWTIAQALERAPQLEPFGLAWLEEPIRADRPRQEWKQLHETIR 246 LR L+G DG L D NQ WT+ QA + PF ++W+EEPI ADRP +EW L E Sbjct: 184 LRDLLGSDGKLMLDANQSWTMDQAKHMIQNINPFNVSWVEEPIAADRPDEEWYGLIEISN 243 Query: 247 FPLAAGENIASHAGFAEVLSEPVLGIVQPDIAKWGGLSACAGIARDILASGKMFCPHYLG 306 PLAAGENI S F ++S LGIVQPD+AKWGGL+ +A+ I+A+ K +CPH+LG Sbjct: 244 MPLAAGENIFSKDRFKHIISTGYLGIVQPDLAKWGGLTETVPLAKAIMAAKKSYCPHFLG 303 Query: 307 AGIGLLASAHLLAGVGGEGLLEVDSNENPLRDQFCGPVLDVSDGMIELGDGAGLGITPDL 366 G+GL+ASAH LA VGG+G LE+D N NPLR S ++LGD G+G+ P+L Sbjct: 304 GGVGLMASAHALAAVGGDGFLELDCNPNPLRTFIASDFFKSSMSCMDLGDEPGIGVVPEL 363 Query: 367 ASIERYRT 374 +I+ YR Sbjct: 364 NAIDEYRV 371