BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0047.0012.p (462 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_767435.1| (dimethylallyl)adenosine tRNA methylthiotransfe... 814 0.0 ref|YP_575383.1| tRNA-i(6)A37 modification enzyme MiaB [Nitrobac... 802 0.0 ref|YP_530362.1| (dimethylallyl)adenosine tRNA methylthiotransfe... 792 0.0 ref|ZP_08628073.1| tRNA-i(6)A37 methylthiotransferase [Bradyrhiz... 786 0.0 ref|YP_001202263.1| (dimethylallyl)adenosine tRNA methylthiotran... 784 0.0 >ref|NP_767435.1| (dimethylallyl)adenosine tRNA methylthiotransferase [Bradyrhizobium japonicum USDA 110] sp|Q89W97.1|MIAB_BRAJA RecName: Full=(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB; AltName: Full=tRNA-i(6)A37 methylthiotransferase dbj|BAC46060.1| bll0795 [Bradyrhizobium japonicum USDA 110] Length = 465 Score = 814 bits (2102), Expect = 0.0, Method: Compositional matrix adjust. Identities = 393/449 (87%), Positives = 417/449 (92%) Query: 1 MTTPRKLHIKSYGCQMNVYDAQRMADTLAGEGFVETASAEDADLVILNTCHIREKASEKV 60 MT PRKLHIKSYGCQMNVYDAQRM DTLA EGFVETASAEDADLVILNTCHIREKASEKV Sbjct: 1 MTPPRKLHIKSYGCQMNVYDAQRMVDTLAPEGFVETASAEDADLVILNTCHIREKASEKV 60 Query: 61 YSELGRLRVAKDEAARAGREMKIAVAGCVAQAEGAEIIHRAPTVDIVVGPQSYHHLPELL 120 YSELGRLRVAKDEAAR GR M+IAVAGCVAQAEG EI+ RAP VD+VVGPQSYHHLPELL Sbjct: 61 YSELGRLRVAKDEAARGGRAMQIAVAGCVAQAEGEEIVRRAPVVDVVVGPQSYHHLPELL 120 Query: 121 KRAKANGRALETEFPVEDKFGFLPQPKPDAIRARGISAFVTVQEGCDKFCTFCVVPYTRG 180 KRA GRA+ETEFP DKFGFL QPKPDAIRARGISAFVTVQEGCDKFCTFCVVPYTRG Sbjct: 121 KRAGNEGRAVETEFPAADKFGFLAQPKPDAIRARGISAFVTVQEGCDKFCTFCVVPYTRG 180 Query: 181 AEVSRPVARIIDDVKQLADNGVREFTLIGQNVNAYHGEGPDGRVWPLGKLLQRLATIPGV 240 AEVSRPVARI+DDVK+LADNGVRE TLIGQNVNAYHG+GPDG+ WPLG+LL+ LA IPG+ Sbjct: 181 AEVSRPVARIVDDVKRLADNGVRELTLIGQNVNAYHGDGPDGKSWPLGRLLEHLAGIPGI 240 Query: 241 MRLRYSTSHPRDVDDALIAAHRDLPELMPFVHLPVQSGADRILAAMNRKHTADDYRRVID 300 RLRYSTSHPRDVDD+LIAAHRDL LMPFVHLPVQSG+DRILAAMNRKHTADDYRRVID Sbjct: 241 ARLRYSTSHPRDVDDSLIAAHRDLAALMPFVHLPVQSGSDRILAAMNRKHTADDYRRVID 300 Query: 301 RFRAARQDIAFSSDFIVGFPGETAEEFSATLALVTQIGYAAAYSFKYSPRPGTPAAEMRE 360 RFRAARQDIAFSSDFIVGFPGE+ ++F ATLALVTQIGYAAAYSFKYS RPGTPAA+M+E Sbjct: 301 RFRAARQDIAFSSDFIVGFPGESEQDFLATLALVTQIGYAAAYSFKYSARPGTPAADMQE 360 Query: 361 TVSAAEMDERLGRLQELIDSQQSAFNRAAIGTTVDVLFERAARNPGQIVGRTAYLQPAHV 420 TVS AEMD+RL RLQELIDSQQSAFN+AAIG+TVDVLFER AR GQIVGRTA+LQPAHV Sbjct: 361 TVSPAEMDQRLERLQELIDSQQSAFNKAAIGSTVDVLFERPARKDGQIVGRTAFLQPAHV 420 Query: 421 MASPDIIGQVLPVRIDSLERYSLIGELAT 449 MASP IIGQ+LPVRIDSLERYS +GEL T Sbjct: 421 MASPGIIGQILPVRIDSLERYSFLGELVT 449 >ref|YP_575383.1| tRNA-i(6)A37 modification enzyme MiaB [Nitrobacter hamburgensis X14] sp|Q1QS74.1|MIAB_NITHX RecName: Full=(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB; AltName: Full=tRNA-i(6)A37 methylthiotransferase gb|ABE60923.1| tRNA-i(6)A37 thiotransferase enzyme MiaB [Nitrobacter hamburgensis X14] Length = 473 Score = 802 bits (2072), Expect = 0.0, Method: Compositional matrix adjust. Identities = 383/445 (86%), Positives = 412/445 (92%) Query: 1 MTTPRKLHIKSYGCQMNVYDAQRMADTLAGEGFVETASAEDADLVILNTCHIREKASEKV 60 MT PRKLHIKSYGCQMNVYDAQRM DTLA EGFVETASAE+ADLVILNTCHIREKASEKV Sbjct: 1 MTPPRKLHIKSYGCQMNVYDAQRMVDTLAAEGFVETASAEEADLVILNTCHIREKASEKV 60 Query: 61 YSELGRLRVAKDEAARAGREMKIAVAGCVAQAEGAEIIHRAPTVDIVVGPQSYHHLPELL 120 YSELGRLR AKD+AAR GREM + VAGCVAQAEG EII RAP VD+VVGPQSYHHLP+LL Sbjct: 61 YSELGRLRAAKDQAARDGREMSVVVAGCVAQAEGDEIIRRAPVVDVVVGPQSYHHLPQLL 120 Query: 121 KRAKANGRALETEFPVEDKFGFLPQPKPDAIRARGISAFVTVQEGCDKFCTFCVVPYTRG 180 RAKA+GRALETEFPVEDKFGFLPQP AIRARGISAFVTVQEGCDKFCTFCVVPYTRG Sbjct: 121 ARAKADGRALETEFPVEDKFGFLPQPSRQAIRARGISAFVTVQEGCDKFCTFCVVPYTRG 180 Query: 181 AEVSRPVARIIDDVKQLADNGVREFTLIGQNVNAYHGEGPDGRVWPLGKLLQRLATIPGV 240 AEVSRPVA+I++DV++LADNGVRE TLIGQNVNAYHG+GPDGR WP GKLL RLA IPG+ Sbjct: 181 AEVSRPVAKIVEDVQRLADNGVREITLIGQNVNAYHGDGPDGRPWPFGKLLHRLADIPGI 240 Query: 241 MRLRYSTSHPRDVDDALIAAHRDLPELMPFVHLPVQSGADRILAAMNRKHTADDYRRVID 300 +RLRYSTSHPRDV+D+LI AHRDL LMPFVHLPVQSG+DRIL AMNR+HTADDYRRV+D Sbjct: 241 VRLRYSTSHPRDVEDSLIEAHRDLGALMPFVHLPVQSGSDRILEAMNRRHTADDYRRVVD 300 Query: 301 RFRAARQDIAFSSDFIVGFPGETAEEFSATLALVTQIGYAAAYSFKYSPRPGTPAAEMRE 360 RFR RQDIAFSSDFIVGFPGET ++F+ATLALVTQIGYA AYSFKYSPRPGTPAAEM+E Sbjct: 301 RFRHVRQDIAFSSDFIVGFPGETEQDFTATLALVTQIGYAGAYSFKYSPRPGTPAAEMKE 360 Query: 361 TVSAAEMDERLGRLQELIDSQQSAFNRAAIGTTVDVLFERAARNPGQIVGRTAYLQPAHV 420 TVS A+MDERL RLQ LIDSQQSAFNRAA+GTTVDVLFERAARNPGQIVGRTAYLQPAHV Sbjct: 361 TVSTADMDERLVRLQNLIDSQQSAFNRAAVGTTVDVLFERAARNPGQIVGRTAYLQPAHV 420 Query: 421 MASPDIIGQVLPVRIDSLERYSLIG 445 +AS DIIGQ+LPV + SLERYSL+G Sbjct: 421 VASADIIGQILPVTVASLERYSLLG 445 >ref|YP_530362.1| (dimethylallyl)adenosine tRNA methylthiotransferase [Rhodopseudomonas palustris BisB18] sp|Q21C43.1|MIAB_RHOPB RecName: Full=(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB; AltName: Full=tRNA-i(6)A37 methylthiotransferase gb|ABD86043.1| tRNA-i(6)A37 thiotransferase enzyme MiaB [Rhodopseudomonas palustris BisB18] Length = 473 Score = 792 bits (2046), Expect = 0.0, Method: Compositional matrix adjust. Identities = 382/448 (85%), Positives = 411/448 (91%) Query: 1 MTTPRKLHIKSYGCQMNVYDAQRMADTLAGEGFVETASAEDADLVILNTCHIREKASEKV 60 MT PRKLHIKS+GCQMNVYDAQRM D LA EGFVETA+A++ADLVILNTCHIREKASEKV Sbjct: 1 MTQPRKLHIKSFGCQMNVYDAQRMVDALAPEGFVETANADEADLVILNTCHIREKASEKV 60 Query: 61 YSELGRLRVAKDEAARAGREMKIAVAGCVAQAEGAEIIHRAPTVDIVVGPQSYHHLPELL 120 YSELGRLRVAKDEAA GR M IAVAGCVAQAEGAEII R P VD+VVGPQSYHHLP+LL Sbjct: 61 YSELGRLRVAKDEAALQGRRMNIAVAGCVAQAEGAEIIRRQPAVDVVVGPQSYHHLPQLL 120 Query: 121 KRAKANGRALETEFPVEDKFGFLPQPKPDAIRARGISAFVTVQEGCDKFCTFCVVPYTRG 180 A GRALETEFPV+DKFGFLP P+PDAIRARGISAFVTVQEGCDKFCTFCVVPYTRG Sbjct: 121 AEAARGGRALETEFPVDDKFGFLPPPQPDAIRARGISAFVTVQEGCDKFCTFCVVPYTRG 180 Query: 181 AEVSRPVARIIDDVKQLADNGVREFTLIGQNVNAYHGEGPDGRVWPLGKLLQRLATIPGV 240 AEVSRPV +I++DV++LADNGVRE TLIGQNVNAYHG+GPDGR WPLG LL LA IPG+ Sbjct: 181 AEVSRPVDKIVEDVRRLADNGVREITLIGQNVNAYHGDGPDGRPWPLGALLHHLAKIPGI 240 Query: 241 MRLRYSTSHPRDVDDALIAAHRDLPELMPFVHLPVQSGADRILAAMNRKHTADDYRRVID 300 +RLRYSTSHPRDVD +LI AHRDLP LMPFVHLPVQSG+D ILAAMNRKH+ADDYRR++D Sbjct: 241 VRLRYSTSHPRDVDQSLIDAHRDLPALMPFVHLPVQSGSDAILAAMNRKHSADDYRRLVD 300 Query: 301 RFRAARQDIAFSSDFIVGFPGETAEEFSATLALVTQIGYAAAYSFKYSPRPGTPAAEMRE 360 RFR+A IAFSSDFIVGFPGET E+F+ATLALVTQIGYA AYSFKYSPRPGTPAAE++E Sbjct: 301 RFRSANPAIAFSSDFIVGFPGETDEDFAATLALVTQIGYAGAYSFKYSPRPGTPAAELQE 360 Query: 361 TVSAAEMDERLGRLQELIDSQQSAFNRAAIGTTVDVLFERAARNPGQIVGRTAYLQPAHV 420 TVSAA MDERL RLQELIDSQQ+AFN A IGTTV+VLFERAARNPGQIVGRTAYLQPAHV Sbjct: 361 TVSAAVMDERLVRLQELIDSQQAAFNAAVIGTTVEVLFERAARNPGQIVGRTAYLQPAHV 420 Query: 421 MASPDIIGQVLPVRIDSLERYSLIGELA 448 MA+PDIIGQVLPVRIDSLERYSLIGELA Sbjct: 421 MAAPDIIGQVLPVRIDSLERYSLIGELA 448 >ref|ZP_08628073.1| tRNA-i(6)A37 methylthiotransferase [Bradyrhizobiaceae bacterium SG-6C] gb|EGP08982.1| tRNA-i(6)A37 methylthiotransferase [Bradyrhizobiaceae bacterium SG-6C] Length = 466 Score = 786 bits (2030), Expect = 0.0, Method: Compositional matrix adjust. Identities = 378/448 (84%), Positives = 406/448 (90%) Query: 1 MTTPRKLHIKSYGCQMNVYDAQRMADTLAGEGFVETASAEDADLVILNTCHIREKASEKV 60 MTTPRKLHIKSYGCQMNVYDAQRM DTL EG+VETA+AEDADLVILNTCHIREKASEKV Sbjct: 1 MTTPRKLHIKSYGCQMNVYDAQRMVDTLTPEGYVETANAEDADLVILNTCHIREKASEKV 60 Query: 61 YSELGRLRVAKDEAARAGREMKIAVAGCVAQAEGAEIIHRAPTVDIVVGPQSYHHLPELL 120 YSELGRLR AKD+AAR GREM+IAVAGCVAQAEG+EII RAP VDIVVGPQSYHHLP+LL Sbjct: 61 YSELGRLRAAKDDAARNGREMRIAVAGCVAQAEGSEIIQRAPVVDIVVGPQSYHHLPQLL 120 Query: 121 KRAKANGRALETEFPVEDKFGFLPQPKPDAIRARGISAFVTVQEGCDKFCTFCVVPYTRG 180 +A+++GRA+ETEFPVEDKF LP PKP IRARGIS+FVTVQEGCDKFCTFCVVPYTRG Sbjct: 121 AKARSDGRAIETEFPVEDKFAALPDPKPAMIRARGISSFVTVQEGCDKFCTFCVVPYTRG 180 Query: 181 AEVSRPVARIIDDVKQLADNGVREFTLIGQNVNAYHGEGPDGRVWPLGKLLQRLATIPGV 240 EVSRPVARIIDDVK+LADNGVRE TLIGQNVNA+HGEG +G W LG+LL RLA IPG+ Sbjct: 181 MEVSRPVARIIDDVKRLADNGVREITLIGQNVNAFHGEGENGEPWSLGRLLHRLADIPGI 240 Query: 241 MRLRYSTSHPRDVDDALIAAHRDLPELMPFVHLPVQSGADRILAAMNRKHTADDYRRVID 300 +RLRYSTSHPRDVDDALI AHRD+P +MPFVHLPVQSG+DRIL AMNRKHTA DY R + Sbjct: 241 LRLRYSTSHPRDVDDALIEAHRDIPAVMPFVHLPVQSGSDRILEAMNRKHTAADYARTVA 300 Query: 301 RFRAARQDIAFSSDFIVGFPGETAEEFSATLALVTQIGYAAAYSFKYSPRPGTPAAEMRE 360 RFR R+DIAFSSDFIVGFPGET E+FSATLALV QI YA AYSFKYSPRPGTPAA+M+E Sbjct: 301 RFRKVREDIAFSSDFIVGFPGETEEDFSATLALVRQISYAGAYSFKYSPRPGTPAADMQE 360 Query: 361 TVSAAEMDERLGRLQELIDSQQSAFNRAAIGTTVDVLFERAARNPGQIVGRTAYLQPAHV 420 V+A MDERL RLQELIDSQQ+AFN AAIG TVDVLFERAARNPGQIVGRTAYLQPAHV Sbjct: 361 MVAATVMDERLARLQELIDSQQAAFNAAAIGETVDVLFERAARNPGQIVGRTAYLQPAHV 420 Query: 421 MASPDIIGQVLPVRIDSLERYSLIGELA 448 MASPDIIGQVLPV IDSLERYSLIG LA Sbjct: 421 MASPDIIGQVLPVAIDSLERYSLIGRLA 448 >ref|YP_001202263.1| (dimethylallyl)adenosine tRNA methylthiotransferase [Bradyrhizobium sp. ORS 278] sp|A4YJD7.1|MIAB_BRASO RecName: Full=(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB; AltName: Full=tRNA-i(6)A37 methylthiotransferase emb|CAL74013.1| Conserved hypothetical protein; putative MiaB-like tRNA modifying enzyme [Bradyrhizobium sp. ORS 278] Length = 467 Score = 784 bits (2024), Expect = 0.0, Method: Compositional matrix adjust. Identities = 378/454 (83%), Positives = 407/454 (89%), Gaps = 1/454 (0%) Query: 1 MTTPRKLHIKSYGCQMNVYDAQRMADTLAGEGFVETASAEDADLVILNTCHIREKASEKV 60 MT PRKLHIKSYGCQMNVYDAQRM DTL EGFVETA A DADLVILNTCHIREKASEKV Sbjct: 1 MTPPRKLHIKSYGCQMNVYDAQRMVDTLGAEGFVETADAGDADLVILNTCHIREKASEKV 60 Query: 61 YSELGRLRVAKDEAARAGREMKIAVAGCVAQAEGAEIIHRAPTVDIVVGPQSYHHLPELL 120 YSELGRLRVAK+EAAR+GR M+IAVAGCVAQAEG EII RAPTVD+VVGPQSYHHLP+LL Sbjct: 61 YSELGRLRVAKEEAARSGRAMQIAVAGCVAQAEGVEIISRAPTVDVVVGPQSYHHLPQLL 120 Query: 121 KRAKANGRALETEFPVEDKFGFLPQPKPDAIRARGISAFVTVQEGCDKFCTFCVVPYTRG 180 +A RA+ETEFP EDKFGFL +P +AIRARG+SAFVTVQEGCDKFCTFCVVPYTRG Sbjct: 121 AQASRGERAIETEFPAEDKFGFLAKPSREAIRARGVSAFVTVQEGCDKFCTFCVVPYTRG 180 Query: 181 AEVSRPVARIIDDVKQLADNGVREFTLIGQNVNAYHGEGPDGRVWPLGKLLQRLATIPGV 240 AE+SRPVARI+DDV QL D+GVRE TLIGQNVNAYHGEGPDGR W LG+LL R+A IPGV Sbjct: 181 AEMSRPVARIVDDVMQLTDSGVREITLIGQNVNAYHGEGPDGRTWTLGRLLYRIAEIPGV 240 Query: 241 MRLRYSTSHPRDVDDALIAAHRDLPELMPFVHLPVQSGADRILAAMNRKHTADDYRRVID 300 R+RYSTSHP DVDD LIAAHRDL +MPFVHLPVQSG+DRILAAMNRKH+A DYRRV+D Sbjct: 241 ARIRYSTSHPNDVDDGLIAAHRDLTAVMPFVHLPVQSGSDRILAAMNRKHSAADYRRVVD 300 Query: 301 RFRAARQDIAFSSDFIVGFPGETAEEFSATLALVTQIGYAAAYSFKYSPRPGTPAAEMRE 360 RFR AR DIAFSSDFIVGFPGET E+F ATLAL+ QIGYAAAYSFKYSPRPGTPAA+M+E Sbjct: 301 RFRGARDDIAFSSDFIVGFPGETEEDFRATLALIDQIGYAAAYSFKYSPRPGTPAADMQE 360 Query: 361 TVSAAEMDERLGRLQELIDSQQSAFNRAAIGTTVDVLFERAARNPGQIVGRTAYLQPAHV 420 VSA EMDERL RLQ LIDSQQ+AFN+AAIG+ VDVLFER AR PGQ+VGRTAYLQPAHV Sbjct: 361 MVSATEMDERLERLQSLIDSQQAAFNKAAIGSVVDVLFEREARKPGQLVGRTAYLQPAHV 420 Query: 421 MASPDIIGQVLPVRIDSLERYSLIGEL-ATRPAP 453 MAS DIIGQVLPVRIDSLERYSL+GEL AT AP Sbjct: 421 MASSDIIGQVLPVRIDSLERYSLLGELVATTAAP 454