BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0043.0016.p (444 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_767615.1| acetyl-CoA synthetase [Bradyrhizobium japonicum... 739 0.0 ref|YP_003318793.1| CoA-binding domain-containing protein [Sphae... 243 7e-62 ref|YP_046482.1| pimeloyl-CoA synthetase [Acinetobacter sp. ADP1... 222 9e-56 ref|YP_846581.1| CoA-binding domain-containing protein [Syntroph... 222 1e-55 ref|YP_584744.1| pimeloyl-CoA synthetase [Cupriavidus metallidur... 220 3e-55 >ref|NP_767615.1| acetyl-CoA synthetase [Bradyrhizobium japonicum USDA 110] dbj|BAC46240.1| bll0975 [Bradyrhizobium japonicum USDA 110] Length = 739 Score = 739 bits (1907), Expect = 0.0, Method: Compositional matrix adjust. Identities = 383/442 (86%), Positives = 408/442 (92%) Query: 1 MEAFASTASHVSHKWAPPADASDIVKSIHAMLHPRNIVLVGATDKPGNYAERIWNNLVKY 60 MEA S A W+P DASDIVK IHAMLHP NIVLVGATDKPGNYAERIWNNLVKY Sbjct: 1 MEARVSAAFSSPRPWSPSPDASDIVKGIHAMLHPHNIVLVGATDKPGNYAERIWNNLVKY 60 Query: 61 GYAGGLYPINAKREAIWGVTCYKDFASLPEKPDHVLVLVPARFAVQVIRDAAAAGARSAT 120 G+ GGLYP+NAKRE IWGV CYKDFA+LPEKPDHVLVLVPARFAVQVIRDAAAAGARSAT Sbjct: 61 GFEGGLYPVNAKRETIWGVPCYKDFAALPEKPDHVLVLVPARFAVQVIRDAAAAGARSAT 120 Query: 121 IVTSGFSELQDEESQRLAVELKQAIKETGLAVTGPNCLGNLSAGENLFTNIDDRIVTMEQ 180 IVTSGFSELQD+ESQ+LA EL+ AI+ETGLAVTGPNCLGNLSAGE LFTNIDDRIVTMEQ Sbjct: 121 IVTSGFSELQDDESQQLAAELQAAIRETGLAVTGPNCLGNLSAGEKLFTNIDDRIVTMEQ 180 Query: 181 GAVAIAGQSGAIVMAIRQALEDRGVGVGYMVTTGNESGLETPDLMSYFAADPSIRVIVVY 240 GAVAIAGQSGAIVMAIRQALEDRGVGVGYMVTTGNE+GLETPDLM YFA DPS++VIVVY Sbjct: 181 GAVAIAGQSGAIVMAIRQALEDRGVGVGYMVTTGNEAGLETPDLMRYFAEDPSVKVIVVY 240 Query: 241 LEGVRNTKVFRDACKAARAAGKPVIALKLGASEGGRAAAMAHTGALAGSIETFDAISTRE 300 LEGVRNTK FRDACKAARAA KPVIALKLGASEGGRAAAMAHTGALAGSIETFDAI+TRE Sbjct: 241 LEGVRNTKAFRDACKAARAASKPVIALKLGASEGGRAAAMAHTGALAGSIETFDAIATRE 300 Query: 301 GVIRVRGLDELIETTECFVHADPPKGNRLAAVSLSGGKRGLLIDAFYSAGLNFAPLSPNA 360 GVIRV GLDELIETTECFVHA P G+RLAAV+LSGGKRG+LIDAFY+ GLNFAPLSP+ Sbjct: 301 GVIRVGGLDELIETTECFVHAAVPNGDRLAAVTLSGGKRGMLIDAFYAEGLNFAPLSPHV 360 Query: 361 SEQLAKMLGPGSIVGNPLDAGFAAVVDPSVYMKSIKIMIDDPDTDIVIIDAELPKAPHEL 420 S +LA+MLGPGSIVGNPLDAGFAAVVDPSVYMKSIK+MIDDPD DIVIIDAELPKAPHEL Sbjct: 361 SSELARMLGPGSIVGNPLDAGFAAVVDPSVYMKSIKLMIDDPDIDIVIIDAELPKAPHEL 420 Query: 421 RERNLRIVNEMAGQANKPFFFL 442 RERNLRIV+EMA +A+KP ++ Sbjct: 421 RERNLRIVDEMASRASKPVIYI 442 >ref|YP_003318793.1| CoA-binding domain-containing protein [Sphaerobacter thermophilus DSM 20745] gb|ACZ37971.1| CoA-binding domain protein [Sphaerobacter thermophilus DSM 20745] Length = 743 Score = 243 bits (619), Expect = 7e-62, Method: Compositional matrix adjust. Identities = 140/399 (35%), Positives = 216/399 (54%), Gaps = 9/399 (2%) Query: 20 DASDIVKSIHAMLHPRNIVLVGATDKPGNYAERIWNNLVKYGYAGGLYPINAKREAIWGV 79 D + ++ A+LHPR I +VGA+ K G +A I LV G+ G +YP+N + I G+ Sbjct: 17 DDAAFASAMRALLHPRRIAIVGASPKRG-FANNIQRRLVDCGFEGEVYPVNPNYDEILGL 75 Query: 80 TCYKDFASLPEKPDHVLVLVPARFAVQVIRDAAAAGARSATIVTSGFSELQDEESQRLAV 139 CY ++P D +V+VP+R V+ AG + I+TSGF+E Q++E+ Sbjct: 76 PCYPSLEAIPGGVDLAVVVVPSRLIPGVLESCERAGVGAVNIITSGFAEKQEDET---GP 132 Query: 140 ELKQAIKE----TGLAVTGPNCLGNLSAGENLFTNIDDRIVTMEQGAVAIAGQSGAIVMA 195 E ++AI++ TG+ V GPNCLGN+S + + + +G +A+A QSG + + Sbjct: 133 ERQRAIRDFARRTGIRVVGPNCLGNISVPNRMVAS-SGAYPPLNRGPIALALQSGLLAYS 191 Query: 196 IRQALEDRGVGVGYMVTTGNESGLETPDLMSYFAADPSIRVIVVYLEGVRNTKVFRDACK 255 + DRG+G Y+VT GNE+ ++ D++ Y+ D RVI ++E R + + + Sbjct: 192 LVLPAHDRGIGFTYVVTLGNEADIDVVDMIRYYLDDEETRVIGCFVEQFRRLERLLEMAE 251 Query: 256 AARAAGKPVIALKLGASEGGRAAAMAHTGALAGSIETFDAISTREGVIRVRGLDELIETT 315 A GKP++ LK+G SE GR AA+AHTG+L GS E DA+ + G+IRV LDE+IET Sbjct: 252 RAADLGKPIVVLKVGRSEAGRRAALAHTGSLVGSDEIVDAVMRQHGIIRVYSLDEMIETL 311 Query: 316 ECFVHADPPKGNRLAAVSLSGGKRGLLIDAFYSAGLNFAPLSPNASEQLAKMLGPGSIVG 375 F P G + +SGG GL+ D G+ F L+P E++ ++ VG Sbjct: 312 ALFHSRRRPAGRGVGIAVVSGGAAGLIADLGADIGVEFPSLAPETVEKMNAVIPEYGTVG 371 Query: 376 NPLDAGFAAVVDPSVYMKSIKIMIDDPDTDIVIIDAELP 414 NPLD A P + + M +DP+ +V+ P Sbjct: 372 NPLDYTGQAAQQPEILDGCLAAMAEDPNIHVVVYGQAYP 410 >ref|YP_046482.1| pimeloyl-CoA synthetase [Acinetobacter sp. ADP1] emb|CAG68660.1| pimeloyl-CoA synthetase [Acinetobacter sp. ADP1] Length = 701 Score = 222 bits (566), Expect = 9e-56, Method: Compositional matrix adjust. Identities = 133/380 (35%), Positives = 208/380 (54%), Gaps = 8/380 (2%) Query: 28 IHAMLHPRNIVLVGATDKPGNYAERIWNNLVKYGYAGGLYPINAKREAIWGVTCYKDFAS 87 + A+L+P ++ L+GA+D P R L + G+ G ++P+N R+ + G+ Y D AS Sbjct: 6 LDALLNPASVALIGASDNPARIGGRPLRYLKESGFEGEIFPVNPNRDRVQGMLAYADLAS 65 Query: 88 LPEKPDHVLVLVPARFAVQVIRDAAAAGARSATIVTSGFSELQDEESQRLAVELKQAIKE 147 LP+ D L+ VPA VQ +R+ A G ++A + ++GF+E E+ ++ E+ A KE Sbjct: 66 LPKAADVALLAVPASATVQAVRECAQKGIQTAIVFSAGFAE-AGEDGHQMQQEMLNAAKE 124 Query: 148 TGLAVTGPNCLGNLSAGENLF----TNIDDRIVTMEQGAVAIAGQSGAIVMAIRQALEDR 203 + L + GPNCLG +A + + T +D + E GAV+I QSGA I R Sbjct: 125 SNLRLLGPNCLGVFNAYKKFYGTFSTVLDGDFI--EPGAVSIVSQSGAYGSHIAHLCRQR 182 Query: 204 GVGVGYMVTTGNESGLETPDLMSYFAADPSIRVIVVYLEGVRNTKVFRDACKAARAAGKP 263 G+G+GY VTTGNE ++ D +++ P ++V++ Y EG+R+ F A + A+ Sbjct: 183 GLGIGYWVTTGNECDIDLSDALNWVVDQPEVKVVLAYAEGIRHRDRFIQALEKAQRNNVA 242 Query: 264 VIALKLGASEGGRAAAMAHTGALAGSIETFDAISTREGVIRVRGLDELIETTECFVHADP 323 +I +K+G SE G AA +HT ALAGS FDA+ + GVIR + E I+ A Sbjct: 243 IIFMKVGRSEVGAKAASSHTAALAGSNAVFDAVLRQYGVIRAKTTTEQIDIAYAVTRAGH 302 Query: 324 PKGNRLAAVSLSGGKRGLLIDAFYSAGLNFAPLSPNASEQLAKMLGPGSIVGNPLDAGFA 383 + NRL + SGG + D +AGL+ AP+ A ++L ++L S V NP+DA Sbjct: 303 IEHNRLGVFTFSGGFGIQMADDAEAAGLDVAPMPEAAQDELKQLLPYASPV-NPVDATAQ 361 Query: 384 AVVDPSVYMKSIKIMIDDPD 403 A+ D + I+ M+ D Sbjct: 362 ALTDLPMMTSFIQTMLKKGD 381 >ref|YP_846581.1| CoA-binding domain-containing protein [Syntrophobacter fumaroxidans MPOB] gb|ABK18146.1| CoA-binding domain protein [Syntrophobacter fumaroxidans MPOB] Length = 669 Score = 222 bits (566), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 130/390 (33%), Positives = 201/390 (51%), Gaps = 27/390 (6%) Query: 30 AMLHPRNIVLVGATDKPGNYAERIWNNLVKYGYAGGLYPINAKREAIWGVTCYKDFASLP 89 + HP ++ ++GA+D G I NL++ G+ G LYPIN K E I G+TC+K+ +P Sbjct: 8 SFFHPSSVAVIGASDSAGKLGHEILKNLIEGGFPGALYPINPKAEKILGLTCFKNVKDIP 67 Query: 90 EKPDHVLVLVPARFAVQVIRDAAAAGARSATIVTSGFSELQDEESQRLAVELKQAIKETG 149 ++ D +V++PAR Q +RD G + A IVT GFSE E +L EL + Sbjct: 68 DQVDMAVVIIPARMVPQAVRDCGEKGVKGAVIVTGGFSE-AGAEGAKLQEELVRVASNYD 126 Query: 150 LAVTGPNCLG------NLSAGENLFTNIDDRIVTMEQGAVAIAGQSGAIVMAIRQALEDR 203 + + GPNC G + A L T+ +G VA+ QSG + A+ Sbjct: 127 IRLVGPNCQGVNNPHHPMCASWPLLTH---------KGRVAVISQSGTVGAAMMDWFSVE 177 Query: 204 GVGVGYMVTTGNESGLETPDLMSYFAADPSIRVIVVYLEGVRNTKVFRDACKAARAAGKP 263 +GV V+ GN + ++ DL+ YF DP+ +VI VY+EG+++ + FRD KP Sbjct: 178 ELGVSSFVSLGNRADIDEVDLIEYFEGDPNTKVIAVYIEGIKDPQRFRDVLS---TLSKP 234 Query: 264 VIALKLGASEGGRAAAMAHTGALAGSIETFDAISTREGVIRVRGLDELIETTECFVHADP 323 ++ LK G + GGR AA +HT +LAG+ + ++ R GV R ++E + + F + P Sbjct: 235 LVVLKSGRTPGGRVAAESHTKSLAGADAIYASLFRRHGVCRAETIEEFFDFAKGFAYLKP 294 Query: 324 PKGNRLAAVSLSGGKRGLLIDAFYSAGLNFAPLSPNASEQLAKMLGPGSIVGNPLDAG-- 381 P GNR+ ++ SGG L DA GL+ + L + QL ++ P +I NPLD Sbjct: 295 PSGNRILYITTSGGSAILATDAAEQEGLSVSALPEELAGQLEGVIPPHAIRSNPLDLTGD 354 Query: 382 -----FAAVVDPSV-YMKSIKIMIDDPDTD 405 F V+ + Y ++ I+ DP D Sbjct: 355 ANAKMFQEVIQRARPYYDTLGIIFGDPILD 384 >ref|YP_584744.1| pimeloyl-CoA synthetase [Cupriavidus metallidurans CH34] gb|ABF09475.1| pimeloyl-CoA synthetase [Cupriavidus metallidurans CH34] Length = 708 Score = 220 bits (561), Expect = 3e-55, Method: Compositional matrix adjust. Identities = 136/386 (35%), Positives = 201/386 (52%), Gaps = 4/386 (1%) Query: 26 KSIHAMLHPRNIVLVGATDKPGNYAERIWNNLVKYG-YAGGLYPINAKREAIWGVTCYKD 84 + + ++ PR+I LVGA+D+ G+ R NL+ + + G +Y +NA + + G + D Sbjct: 5 QDLSRLVSPRSIALVGASDRSGSIGARALENLMDHSDFKGDVYLVNATKPEVAGRKAWPD 64 Query: 85 FASLPEKPDHVLVLVPARFAVQVIRDAAAAGARSATIVTSGFSELQDEESQRLAVELKQA 144 ASLP PD LV VPA + V+ G R A I+TSGF E EE + +K+ Sbjct: 65 VASLPSVPDTALVAVPAEHVLAVLEQCGEKGVRFAIILTSGFGE-AGEEGKAAEQTMKEI 123 Query: 145 IKETGLAVTGPNCLGNLSAGENL-FTNIDDRIVTMEQGAVAIAGQSGAIVMAIRQALEDR 203 + TG+ + GPNC G + E L FT + G V +A Q G + + QA+ DR Sbjct: 124 VARTGMRIYGPNCPGLTNITERLGFTFSPSFRHDLRGGPVGLATQGGGLGRNVLQAM-DR 182 Query: 204 GVGVGYMVTTGNESGLETPDLMSYFAADPSIRVIVVYLEGVRNTKVFRDACKAARAAGKP 263 G+GV ++GNE L+ D + Y A P I+VI LEGV + + F A + A GKP Sbjct: 183 GIGVALWSSSGNEVDLQVSDFIHYMADAPDIKVIATLLEGVNDGRKFVAAVQHAARKGKP 242 Query: 264 VIALKLGASEGGRAAAMAHTGALAGSIETFDAISTREGVIRVRGLDELIETTECFVHADP 323 V+ LK+G SE GR AA +HT ++ GS E A+ + GV+ V +DELI+T F P Sbjct: 243 VVVLKVGRSEYGRKAAQSHTASITGSAEVNSAVFAQLGVVEVDDIDELIDTAWLFARGMP 302 Query: 324 PKGNRLAAVSLSGGKRGLLIDAFYSAGLNFAPLSPNASEQLAKMLGPGSIVGNPLDAGFA 383 +G LA S SGG L D +AGL A +P L L + + NP+D A Sbjct: 303 RQGGALAIYSGSGGTAALCADMVGNAGLKLAEFAPETLAVLRDALPSYAAIDNPIDTTTA 362 Query: 384 AVVDPSVYMKSIKIMIDDPDTDIVII 409 + P + ++ + +DP +V++ Sbjct: 363 VMAKPELARTCLQAVCNDPSVSLVLL 388