BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0040.0030.p (410 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003743146.1| major Facilitator Superfamily protein [Erwin... 366 3e-99 ref|YP_001898979.1| major facilitator superfamily protein [Ralst... 363 4e-98 ref|YP_002981407.1| major facilitator superfamily protein [Ralst... 362 5e-98 ref|ZP_07676763.1| purine ribonucleoside efflux pump NepI [Ralst... 361 1e-97 ref|YP_001341617.1| major facilitator transporter [Marinomonas s... 355 5e-96 >ref|YP_003743146.1| major Facilitator Superfamily protein [Erwinia billingiae Eb661] emb|CAX61299.1| Major Facilitator Superfamily protein [Erwinia billingiae Eb661] Length = 398 Score = 366 bits (940), Expect = 3e-99, Method: Compositional matrix adjust. Identities = 192/382 (50%), Positives = 268/382 (70%) Query: 27 SPERPAWGAVAAMMLGVTALLTAEFLPSGLLTPMARDLGVSPGLAGQAVTATSLAGLVGA 86 + E+P WGAV +M LGV L+TAEFLP+ LLTPMA LGV+ G+ GQAVT T++ L+ + Sbjct: 10 AEEKPQWGAVFSMALGVFGLVTAEFLPASLLTPMAASLGVTEGVTGQAVTITAVFALLTS 69 Query: 87 LSTPTLTRRFNRKPVLLSFSILLVISNLLVALAPSITFLLAARIVLGLAIGGFWAMAAAT 146 + + TR +R+ V+L FS++L++SNLLVA +P++ F+LA R++LG+AIGGFW +AAAT Sbjct: 70 FFSASATRNLDRRWVMLMFSVMLIVSNLLVAFSPNLGFILAGRVLLGIAIGGFWTLAAAT 129 Query: 147 TIRLVPPSLVARALSIVMSGIPAAMIIAVPLGSYLGEVASWRSVFLLATALGAAVFVIQA 206 T+RLVP +++ RALSIV SG+ A I+A P+GSY G + WR+VF+ A+ +G Q Sbjct: 130 TMRLVPEAMIPRALSIVFSGVSVATIVAAPMGSYFGHLIGWRNVFVAASVIGLLALAWQV 189 Query: 207 LALPRLAPRGEAGLDTLIDILTRPGIGAGILAATLVFTGHFGYFGYIRPFLESVTGLGAA 266 ALP++ ++ ++ RPG+ +LA LVF+GHF +F Y+RPFLESVTG+G Sbjct: 190 AALPKMPSTQVGKSSSMFALIRRPGMQTALLAIILVFSGHFAFFTYLRPFLESVTGVGVN 249 Query: 267 GVAATLLAFGVANYLGSFASGLRAERKLKLTLIAMPLAISVIGLLLSVLGKDVIPALVLV 326 G++ LL FGVAN+ G+ +G+ ER L TLI MPLA+ ++G+ L V+G + VLV Sbjct: 250 GLSVILLGFGVANFAGTLIAGILLERNLHRTLIGMPLAMGLLGVGLVVMGSAPLVDAVLV 309 Query: 327 ALWGFAFSGVPVATTTWITRMVPDETESGTGLTVASIFLGITIGAAGGGLVLDLMGAAAV 386 ALWG AF VPV +TWITR +PDE ESG GL VASI L ITIGAA GG++ ++ GA V Sbjct: 310 ALWGMAFGAVPVGWSTWITRTMPDEAESGGGLLVASIQLAITIGAAAGGVIFNITGAKGV 369 Query: 387 FVAGAIPLVLAALLIATKVRTR 408 F+ A+ L++A L I +RT+ Sbjct: 370 FMGSAMALLIATLTIYAALRTK 391 >ref|YP_001898979.1| major facilitator superfamily protein [Ralstonia pickettii 12J] gb|ACD26547.1| major facilitator superfamily MFS_1 [Ralstonia pickettii 12J] Length = 403 Score = 363 bits (931), Expect = 4e-98, Method: Compositional matrix adjust. Identities = 211/383 (55%), Positives = 264/383 (68%) Query: 26 ASPERPAWGAVAAMMLGVTALLTAEFLPSGLLTPMARDLGVSPGLAGQAVTATSLAGLVG 85 A+P PAWGAV AM LGV L+TAEFLP+ LLTPMA LGV+ G+AGQAVTAT+ LV Sbjct: 12 AAPREPAWGAVFAMALGVFGLVTAEFLPASLLTPMAESLGVTEGVAGQAVTATATVALVT 71 Query: 86 ALSTPTLTRRFNRKPVLLSFSILLVISNLLVALAPSITFLLAARIVLGLAIGGFWAMAAA 145 +L T +TR +R+ VLL+FS+LLV SNL VA A S+T +L R+VLG+A+GGFW MA A Sbjct: 72 SLLTAAVTRSIDRRRVLLAFSVLLVASNLAVAFATSLTTILIGRVVLGIALGGFWTMATA 131 Query: 146 TTIRLVPPSLVARALSIVMSGIPAAMIIAVPLGSYLGEVASWRSVFLLATALGAAVFVIQ 205 T +RLVPP+LV RALSI+ SG+ A I A PLGSY G + WR+VFL + LG FV Q Sbjct: 132 TAMRLVPPALVPRALSIIFSGVAVATIAAAPLGSYFGHLIGWRNVFLASAGLGVMAFVSQ 191 Query: 206 ALALPRLAPRGEAGLDTLIDILTRPGIGAGILAATLVFTGHFGYFGYIRPFLESVTGLGA 265 LP +AP G L TLID+L RP +G G+ A LVFTGHF +F Y+RPFLE V G+G Sbjct: 192 VSTLPSMAPSGTTRLRTLIDVLRRPSVGIGMFATILVFTGHFAFFTYLRPFLEQVAGVGV 251 Query: 266 AGVAATLLAFGVANYLGSFASGLRAERKLKLTLIAMPLAISVIGLLLSVLGKDVIPALVL 325 G++A LL +G+AN++G+ +G E +L+ LIAMP + V+G+ L LG+ + VL Sbjct: 252 NGLSAILLGYGIANFVGTSLAGRVLEHRLRPMLIAMPALMVVLGIALVALGRAPVIDAVL 311 Query: 326 VALWGFAFSGVPVATTTWITRMVPDETESGTGLTVASIFLGITIGAAGGGLVLDLMGAAA 385 +ALWG AF GVPVA +TW+TR VPDE ES GL VA++ L I GAA GGLV D GAA Sbjct: 312 IALWGMAFGGVPVAWSTWVTRTVPDEAESAGGLIVAAVQLAIATGAAAGGLVFDANGAAG 371 Query: 386 VFVAGAIPLVLAALLIATKVRTR 408 VF+ A+ L A I T V R Sbjct: 372 VFIGAAVVLATAVATIVTGVPKR 394 >ref|YP_002981407.1| major facilitator superfamily protein [Ralstonia pickettii 12D] gb|ACS62735.1| major facilitator superfamily MFS_1 [Ralstonia pickettii 12D] Length = 403 Score = 362 bits (930), Expect = 5e-98, Method: Compositional matrix adjust. Identities = 211/383 (55%), Positives = 264/383 (68%) Query: 26 ASPERPAWGAVAAMMLGVTALLTAEFLPSGLLTPMARDLGVSPGLAGQAVTATSLAGLVG 85 A+P PAWGAV AM LGV L+TAEFLP+ LLTPMA LGV+ G+AGQAVTAT+ LV Sbjct: 12 AAPREPAWGAVFAMALGVFGLVTAEFLPASLLTPMAESLGVTEGVAGQAVTATATVALVT 71 Query: 86 ALSTPTLTRRFNRKPVLLSFSILLVISNLLVALAPSITFLLAARIVLGLAIGGFWAMAAA 145 +L T +TR +R+ VLL+FS+LLV SNL VA A ++T +L R+VLG+A+GGFW MA A Sbjct: 72 SLLTAAVTRSIDRRRVLLAFSVLLVASNLAVAFATNLTTILIGRVVLGIALGGFWTMATA 131 Query: 146 TTIRLVPPSLVARALSIVMSGIPAAMIIAVPLGSYLGEVASWRSVFLLATALGAAVFVIQ 205 T +RLVPP+LV RALSI+ SG+ A I A PLGSY G + WR+VFL A LG FV Q Sbjct: 132 TAMRLVPPALVPRALSIIFSGVAVATIAAAPLGSYFGHLIGWRNVFLAAAGLGVMAFVSQ 191 Query: 206 ALALPRLAPRGEAGLDTLIDILTRPGIGAGILAATLVFTGHFGYFGYIRPFLESVTGLGA 265 LP +AP G L TLID+L RP +G G+ A LVFTGHF +F Y+RPFLE V G+G Sbjct: 192 VSTLPSMAPSGTTRLRTLIDVLRRPSVGIGMFATILVFTGHFAFFTYLRPFLEQVAGVGV 251 Query: 266 AGVAATLLAFGVANYLGSFASGLRAERKLKLTLIAMPLAISVIGLLLSVLGKDVIPALVL 325 G++A LL +G+AN++G+ +G E +L+ LIAMP + V+G+ L LG+ + VL Sbjct: 252 NGLSAILLGYGIANFVGTSLAGRVLEHRLRPMLIAMPALMVVLGIALVSLGRAPVIDAVL 311 Query: 326 VALWGFAFSGVPVATTTWITRMVPDETESGTGLTVASIFLGITIGAAGGGLVLDLMGAAA 385 +ALWG AF GVPVA +TW+TR VPDE ES GL VA++ L I GAA GGLV D GAA Sbjct: 312 IALWGMAFGGVPVAWSTWVTRTVPDEAESAGGLIVAAVQLAIATGAAAGGLVFDANGAAG 371 Query: 386 VFVAGAIPLVLAALLIATKVRTR 408 VF+ A+ L A I T V R Sbjct: 372 VFIGAAVVLATAVATIVTGVPKR 394 >ref|ZP_07676763.1| purine ribonucleoside efflux pump NepI [Ralstonia sp. 5_7_47FAA] gb|EFP64766.1| purine ribonucleoside efflux pump NepI [Ralstonia sp. 5_7_47FAA] Length = 403 Score = 361 bits (927), Expect = 1e-97, Method: Compositional matrix adjust. Identities = 210/383 (54%), Positives = 265/383 (69%) Query: 26 ASPERPAWGAVAAMMLGVTALLTAEFLPSGLLTPMARDLGVSPGLAGQAVTATSLAGLVG 85 A+P PAWGAV AM LGV L+TAEFLP+ LLTPMA LGV+ G+AGQAVTAT+ LV Sbjct: 12 AAPREPAWGAVFAMALGVFGLVTAEFLPASLLTPMAESLGVTEGVAGQAVTATATVALVT 71 Query: 86 ALSTPTLTRRFNRKPVLLSFSILLVISNLLVALAPSITFLLAARIVLGLAIGGFWAMAAA 145 +L T +TR +R+ VLL+FS+LLV SNL VA A ++T +L R+VLG+A+GGFW MA A Sbjct: 72 SLLTAAVTRSIDRRRVLLAFSVLLVASNLAVAFATNLTTILIGRVVLGIALGGFWTMATA 131 Query: 146 TTIRLVPPSLVARALSIVMSGIPAAMIIAVPLGSYLGEVASWRSVFLLATALGAAVFVIQ 205 T +RLVPP+LV RALSI+ SG+ A I A PLGSY G + WR+VFL A LG FV Q Sbjct: 132 TAMRLVPPALVPRALSIIFSGVAVATIAAAPLGSYFGHLIGWRNVFLAAAGLGVIAFVSQ 191 Query: 206 ALALPRLAPRGEAGLDTLIDILTRPGIGAGILAATLVFTGHFGYFGYIRPFLESVTGLGA 265 LP +AP G L TLID+L RP +G G+ A LVFTGHF +F Y+RPFLE V G+G Sbjct: 192 VATLPSMAPSGTTRLRTLIDVLRRPSVGIGMFATILVFTGHFAFFTYLRPFLEQVAGVGV 251 Query: 266 AGVAATLLAFGVANYLGSFASGLRAERKLKLTLIAMPLAISVIGLLLSVLGKDVIPALVL 325 G++A LL +G+AN++G+ +G E +L+ LIAMP + V+G+ L LG+ + VL Sbjct: 252 NGLSAILLGYGIANFVGTSLAGRVLEHRLRPMLIAMPALMVVLGIALVSLGRAPVIDAVL 311 Query: 326 VALWGFAFSGVPVATTTWITRMVPDETESGTGLTVASIFLGITIGAAGGGLVLDLMGAAA 385 +ALWG AF GVPVA +TW+TR VPDE ES GL VA++ L I GAA GG+V D GAA Sbjct: 312 IALWGMAFGGVPVAWSTWVTRTVPDEAESAGGLIVAAVQLAIATGAAAGGVVFDANGAAG 371 Query: 386 VFVAGAIPLVLAALLIATKVRTR 408 VF+ A+ L +A I T V R Sbjct: 372 VFIGAAVVLAIAVATIVTGVPKR 394 >ref|YP_001341617.1| major facilitator transporter [Marinomonas sp. MWYL1] gb|ABR71682.1| major facilitator superfamily MFS_1 [Marinomonas sp. MWYL1] Length = 401 Score = 355 bits (912), Expect = 5e-96, Method: Compositional matrix adjust. Identities = 202/369 (54%), Positives = 258/369 (69%), Gaps = 1/369 (0%) Query: 20 KVGDKPASPER-PAWGAVAAMMLGVTALLTAEFLPSGLLTPMARDLGVSPGLAGQAVTAT 78 V PA + PAWGAV AM LGV L+TAEFLP+ LLTPMA DL ++ G+AGQAV+AT Sbjct: 4 TVNASPAQVQDVPAWGAVIAMTLGVFGLVTAEFLPASLLTPMASDLSITEGMAGQAVSAT 63 Query: 79 SLAGLVGALSTPTLTRRFNRKPVLLSFSILLVISNLLVALAPSITFLLAARIVLGLAIGG 138 ++ + +L T T+TR +R+ VLL FS+LL+ISN+LVALAP+ LLA R++LG+A+GG Sbjct: 64 AILAVFASLLTATVTRSMDRRYVLLGFSVLLIISNILVALAPNFFLLLAGRVLLGIALGG 123 Query: 139 FWAMAAATTIRLVPPSLVARALSIVMSGIPAAMIIAVPLGSYLGEVASWRSVFLLATALG 198 FW MAAA +RLVP + RAL+I+ SG+ AA I A PLGSYLG WR+VFLL LG Sbjct: 124 FWTMAAAVVMRLVPDKDIPRALAILFSGVSAATIFAAPLGSYLGGTIGWRNVFLLVALLG 183 Query: 199 AAVFVIQALALPRLAPRGEAGLDTLIDILTRPGIGAGILAATLVFTGHFGYFGYIRPFLE 258 V +Q LPR+ PR A L TLI ++ RP + G+L L+FTGHF F Y+RPFLE Sbjct: 184 ILVLAVQFATLPRMQPRSRATLGTLIAVMNRPRMKFGLLMLVLIFTGHFALFTYVRPFLE 243 Query: 259 SVTGLGAAGVAATLLAFGVANYLGSFASGLRAERKLKLTLIAMPLAISVIGLLLSVLGKD 318 +VTG+ AG++ LL FGVAN++G++ +G R L+LTL MPL I ++G+ + + Sbjct: 244 NVTGVATAGISGILLGFGVANFIGTYIAGALIARSLRLTLTLMPLIIGLVGIGMVSIQGA 303 Query: 319 VIPALVLVALWGFAFSGVPVATTTWITRMVPDETESGTGLTVASIFLGITIGAAGGGLVL 378 V P VLVA+WG AF GVPVA +TWITR VPDE ESG GL VA+I +GI GAA GGLV Sbjct: 304 VTPTAVLVAIWGMAFGGVPVAWSTWITRSVPDEAESGGGLLVAAIQVGIATGAAAGGLVF 363 Query: 379 DLMGAAAVF 387 D+ GA VF Sbjct: 364 DVSGALGVF 372