BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0253.p (331 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_08628273.1| biotin synthase [Bradyrhizobiaceae bacterium ... 572 e-161 ref|NP_768735.1| biotin synthetase [Bradyrhizobium japonicum USD... 558 e-157 ref|YP_575636.1| biotin synthase [Nitrobacter hamburgensis X14] ... 558 e-157 ref|YP_001238815.1| biotin synthetase [Bradyrhizobium sp. BTAi1]... 556 e-156 ref|ZP_07027270.1| biotin synthase [Afipia sp. 1NLS2] >gi|298590... 545 e-153 >ref|ZP_08628273.1| biotin synthase [Bradyrhizobiaceae bacterium SG-6C] gb|EGP09182.1| biotin synthase [Bradyrhizobiaceae bacterium SG-6C] Length = 358 Score = 572 bits (1475), Expect = e-161, Method: Compositional matrix adjust. Identities = 266/313 (84%), Positives = 294/313 (93%) Query: 18 PRKTWERAEAEALYNLPFADLMFQAQGIHRANFNPNHVETASLLSIKTGGCPEDCGYCSQ 77 PR W RA+A+ALY+LPFADL+FQAQ IHR NF+PNHVETASLLSIKTGGCPEDCGYCSQ Sbjct: 15 PRNDWTRAQAQALYDLPFADLIFQAQSIHRRNFDPNHVETASLLSIKTGGCPEDCGYCSQ 74 Query: 78 SAHYDTGLKATRLMPRDEVVAVAQRAKDAGATRFCMAAAWRSPKDRDLDQVCDMVSAVKG 137 SA YDTGLKA++LM +D+VVA A+RA+DAGA RFCMAAAWR+PKD+DLD+VC+M++AVKG Sbjct: 75 SAKYDTGLKASKLMEQDDVVATAKRARDAGAERFCMAAAWRNPKDKDLDKVCEMITAVKG 134 Query: 138 LGMETCVTLGMLTPKQAERLSDAGLDFYNHNVDTSPEFYDKIITTRTLQDRIDTLAHVRD 197 LGMETCVTLGMLT KQA+RL DAGLDFYNHNVDTSPEFY KIITTRT+QDRIDTLAH R+ Sbjct: 135 LGMETCVTLGMLTDKQAQRLHDAGLDFYNHNVDTSPEFYGKIITTRTMQDRIDTLAHARE 194 Query: 198 AGIKVCCGGIIGMGEQVDDRLGMLMLLANLPSHPESVPINLWNEVKGVPVNDSAERPDPI 257 AG+KVCCGGIIGMGEQVDDRLGML LLANLP HPESVPIN+WNEVKGVPVND+AERPDPI Sbjct: 195 AGLKVCCGGIIGMGEQVDDRLGMLALLANLPEHPESVPINMWNEVKGVPVNDTAERPDPI 254 Query: 258 ALVRLVATARIMMPKSVVRLSAGRQYMTDELQAMCFLAGANSIFIGDVLLTTKNPQRDRD 317 ALVR+VA ARIMMP+SVVRLSAGRQYMTDELQA+CF+AGANSIFIGDVLLTTKNPQ +RD Sbjct: 255 ALVRMVAVARIMMPRSVVRLSAGRQYMTDELQALCFVAGANSIFIGDVLLTTKNPQTERD 314 Query: 318 ANLLDRLGINSAL 330 ANLLDRLGI S L Sbjct: 315 ANLLDRLGIKSKL 327 >ref|NP_768735.1| biotin synthetase [Bradyrhizobium japonicum USDA 110] sp|Q9AMS7.1|BIOB_BRAJA RecName: Full=Biotin synthase gb|AAG61070.1|AF322013_189 ID897 [Bradyrhizobium japonicum] dbj|BAC47360.1| biotin synthetase [Bradyrhizobium japonicum USDA 110] Length = 331 Score = 558 bits (1439), Expect = e-157, Method: Compositional matrix adjust. Identities = 264/327 (80%), Positives = 294/327 (89%) Query: 5 VEIERTDSRTNAAPRKTWERAEAEALYNLPFADLMFQAQGIHRANFNPNHVETASLLSIK 64 V+++R + A R W EA+ALY+LPFADLM QAQ HR NF+PNHVETASLLSIK Sbjct: 5 VQVQRKKASNGAQVRNHWNVEEAKALYDLPFADLMLQAQRAHRKNFDPNHVETASLLSIK 64 Query: 65 TGGCPEDCGYCSQSAHYDTGLKATRLMPRDEVVAVAQRAKDAGATRFCMAAAWRSPKDRD 124 TGGCPEDCGYCSQSAHY TGLKATRLM +VVA AQRAKDAGATRFCMAAAWR+PKDRD Sbjct: 65 TGGCPEDCGYCSQSAHYATGLKATRLMRCADVVATAQRAKDAGATRFCMAAAWRTPKDRD 124 Query: 125 LDQVCDMVSAVKGLGMETCVTLGMLTPKQAERLSDAGLDFYNHNVDTSPEFYDKIITTRT 184 LD VCDMV+AVKGLGMETCVTLG LTPK A RL++AGLDFYNHNVDTSPEFY KIITTR+ Sbjct: 125 LDSVCDMVNAVKGLGMETCVTLGTLTPKHAARLAEAGLDFYNHNVDTSPEFYSKIITTRS 184 Query: 185 LQDRIDTLAHVRDAGIKVCCGGIIGMGEQVDDRLGMLMLLANLPSHPESVPINLWNEVKG 244 LQDRIDTLAHVRDAGIK+CCGGIIGMGE+V+DRLGML+LLANLP++PESVPINLWN+++G Sbjct: 185 LQDRIDTLAHVRDAGIKICCGGIIGMGERVEDRLGMLVLLANLPNYPESVPINLWNKIEG 244 Query: 245 VPVNDSAERPDPIALVRLVATARIMMPKSVVRLSAGRQYMTDELQAMCFLAGANSIFIGD 304 VPV D+AE PDPIALVRL+ATAR+MMP+SVVRLSAGRQYMTDELQA+CFLAGANSIF+GD Sbjct: 245 VPVEDTAEPPDPIALVRLLATARVMMPRSVVRLSAGRQYMTDELQALCFLAGANSIFVGD 304 Query: 305 VLLTTKNPQRDRDANLLDRLGINSALA 331 VLLTT NP+ DRDA+LL RLGI S LA Sbjct: 305 VLLTTNNPKVDRDADLLARLGITSGLA 331 >ref|YP_575636.1| biotin synthase [Nitrobacter hamburgensis X14] sp|Q1QRH1.1|BIOB_NITHX RecName: Full=Biotin synthase gb|ABE61176.1| biotin synthase [Nitrobacter hamburgensis X14] Length = 340 Score = 558 bits (1437), Expect = e-157, Method: Compositional matrix adjust. Identities = 259/316 (81%), Positives = 289/316 (91%) Query: 15 NAAPRKTWERAEAEALYNLPFADLMFQAQGIHRANFNPNHVETASLLSIKTGGCPEDCGY 74 ++ PR W R+EAE LYNLPFADL+FQAQ +HR +F+PNHVETA+LLSIKTGGCPEDCGY Sbjct: 15 SSQPRHDWTRSEAETLYNLPFADLIFQAQTLHRRHFDPNHVETANLLSIKTGGCPEDCGY 74 Query: 75 CSQSAHYDTGLKATRLMPRDEVVAVAQRAKDAGATRFCMAAAWRSPKDRDLDQVCDMVSA 134 CSQSAHYD+G+KAT+LM R+ V+ A+RA+DAGA+RFCMAAAWR+PKDRDLD+VCDMVSA Sbjct: 75 CSQSAHYDSGVKATKLMDREAVIETARRARDAGASRFCMAAAWRNPKDRDLDRVCDMVSA 134 Query: 135 VKGLGMETCVTLGMLTPKQAERLSDAGLDFYNHNVDTSPEFYDKIITTRTLQDRIDTLAH 194 VK LG+ETC TLGMLTP QA RL DAGLDFYNHNVDTSPEFY IITTRT+QDRIDTLAH Sbjct: 135 VKELGLETCATLGMLTPDQARRLHDAGLDFYNHNVDTSPEFYGNIITTRTMQDRIDTLAH 194 Query: 195 VRDAGIKVCCGGIIGMGEQVDDRLGMLMLLANLPSHPESVPINLWNEVKGVPVNDSAERP 254 R+AG+KVCCGGIIG+GEQV DRLGMLMLLANLP+HPESVPIN+WNEVK VPVND+AERP Sbjct: 195 AREAGLKVCCGGIIGLGEQVGDRLGMLMLLANLPAHPESVPINMWNEVKSVPVNDTAERP 254 Query: 255 DPIALVRLVATARIMMPKSVVRLSAGRQYMTDELQAMCFLAGANSIFIGDVLLTTKNPQR 314 DPIALVR++A ARIMMP+SVVRLSAGRQYMTDELQA+CFLAGANSIFIGDVLLTTKNPQ Sbjct: 255 DPIALVRMIAVARIMMPRSVVRLSAGRQYMTDELQALCFLAGANSIFIGDVLLTTKNPQT 314 Query: 315 DRDANLLDRLGINSAL 330 RDA LLDRLGI S L Sbjct: 315 ARDAALLDRLGIRSRL 330 >ref|YP_001238815.1| biotin synthetase [Bradyrhizobium sp. BTAi1] sp|A5EFG5.1|BIOB_BRASB RecName: Full=Biotin synthase gb|ABQ34909.1| biotin synthetase (Biotin synthase) [Bradyrhizobium sp. BTAi1] Length = 350 Score = 556 bits (1433), Expect = e-156, Method: Compositional matrix adjust. Identities = 267/329 (81%), Positives = 289/329 (87%), Gaps = 3/329 (0%) Query: 6 EIERTDSRTNAAP---RKTWERAEAEALYNLPFADLMFQAQGIHRANFNPNHVETASLLS 62 E+ R S AA R W R EAEALY LPF DLMF+AQ +HR +F+P +ETASLLS Sbjct: 3 EVRRPSSSQPAADGAIRHDWTREEAEALYALPFPDLMFRAQSVHRRHFDPTRLETASLLS 62 Query: 63 IKTGGCPEDCGYCSQSAHYDTGLKATRLMPRDEVVAVAQRAKDAGATRFCMAAAWRSPKD 122 IKTGGCPEDCGYCSQSAHYDTGLKAT+LM RD VVA AQRAK AGA RFCMAAAWRSPKD Sbjct: 63 IKTGGCPEDCGYCSQSAHYDTGLKATKLMDRDTVVATAQRAKAAGAERFCMAAAWRSPKD 122 Query: 123 RDLDQVCDMVSAVKGLGMETCVTLGMLTPKQAERLSDAGLDFYNHNVDTSPEFYDKIITT 182 RDLDQVCDMVSAVK LGMETCVTLGMLTP QA+RL AGLDFYNHNVDTSPEFYD+IITT Sbjct: 123 RDLDQVCDMVSAVKDLGMETCVTLGMLTPPQAQRLKQAGLDFYNHNVDTSPEFYDRIITT 182 Query: 183 RTLQDRIDTLAHVRDAGIKVCCGGIIGMGEQVDDRLGMLMLLANLPSHPESVPINLWNEV 242 RTLQDRIDTLAHVRDAGIKVCCGGIIGMGE VDDRLGM++LLANL +HP+SVPIN+WNEV Sbjct: 183 RTLQDRIDTLAHVRDAGIKVCCGGIIGMGEAVDDRLGMMVLLANLGAHPDSVPINMWNEV 242 Query: 243 KGVPVNDSAERPDPIALVRLVATARIMMPKSVVRLSAGRQYMTDELQAMCFLAGANSIFI 302 KGVPVND+A RPDPIALVRL+A RIMMP+SVVRLSAGRQYM+DELQ++C LAGANSIFI Sbjct: 243 KGVPVNDTAARPDPIALVRLIAVTRIMMPQSVVRLSAGRQYMSDELQSLCLLAGANSIFI 302 Query: 303 GDVLLTTKNPQRDRDANLLDRLGINSALA 331 GDVLLTT NPQ DRDA LL RLG+ SAL+ Sbjct: 303 GDVLLTTANPQADRDAALLARLGMTSALS 331 >ref|ZP_07027270.1| biotin synthase [Afipia sp. 1NLS2] gb|EFI51026.1| biotin synthase [Afipia sp. 1NLS2] Length = 351 Score = 545 bits (1404), Expect = e-153, Method: Compositional matrix adjust. Identities = 251/311 (80%), Positives = 281/311 (90%) Query: 18 PRKTWERAEAEALYNLPFADLMFQAQGIHRANFNPNHVETASLLSIKTGGCPEDCGYCSQ 77 PR W R EA+ALY+LPFADLMFQAQ IHRANF+PNHVETASLLSIKTGGCPEDCGYCSQ Sbjct: 15 PRHDWTRDEAQALYDLPFADLMFQAQSIHRANFDPNHVETASLLSIKTGGCPEDCGYCSQ 74 Query: 78 SAHYDTGLKATRLMPRDEVVAVAQRAKDAGATRFCMAAAWRSPKDRDLDQVCDMVSAVKG 137 SAHY T +KAT+LM D+V+A A+RAK++GA RFCMAAAWR+PKD+DLD+VCDMVS VK Sbjct: 75 SAHYKTDVKATKLMDHDDVIATAKRAKESGANRFCMAAAWRNPKDKDLDKVCDMVSEVKA 134 Query: 138 LGMETCVTLGMLTPKQAERLSDAGLDFYNHNVDTSPEFYDKIITTRTLQDRIDTLAHVRD 197 LG+ETC TLGMLT +QA+RL AGLDFYNHNVDTSPEFY IITTRT+QDRI+TLAH R+ Sbjct: 135 LGLETCATLGMLTREQADRLRAAGLDFYNHNVDTSPEFYGHIITTRTMQDRIETLAHARE 194 Query: 198 AGIKVCCGGIIGMGEQVDDRLGMLMLLANLPSHPESVPINLWNEVKGVPVNDSAERPDPI 257 +G+KVCCGGI+GMGEQV+DRLGMLMLLANL HPESVPINLWNEVKGVPVN +A PDPI Sbjct: 195 SGLKVCCGGIVGMGEQVEDRLGMLMLLANLAEHPESVPINLWNEVKGVPVNGTANAPDPI 254 Query: 258 ALVRLVATARIMMPKSVVRLSAGRQYMTDELQAMCFLAGANSIFIGDVLLTTKNPQRDRD 317 +VR++A ARIMMPKSVVRLSAGRQYMTDE+QA+CFLAGANSIFIGDVLLTTKNPQ D+D Sbjct: 255 EMVRMIAVARIMMPKSVVRLSAGRQYMTDEMQALCFLAGANSIFIGDVLLTTKNPQTDKD 314 Query: 318 ANLLDRLGINS 328 A LLDRLGI S Sbjct: 315 AALLDRLGIKS 325