BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0191.p (376 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_001019373.1| hypothetical protein Mpe_A0176 [Methylibium ... 659 0.0 ref|YP_004235456.1| Saccharopine dehydrogenase [Acidovorax avena... 640 0.0 ref|ZP_00994246.1| Saccharopine dehydrogenase [Janibacter sp. HT... 599 e-169 ref|ZP_08198753.1| hypothetical protein NBCG_03925 [Nocardioidac... 592 e-167 ref|YP_700905.1| hypothetical protein RHA1_ro00915 [Rhodococcus ... 508 e-142 >ref|YP_001019373.1| hypothetical protein Mpe_A0176 [Methylibium petroleiphilum PM1] gb|ABM93138.1| conserved hypothetical protein [Methylibium petroleiphilum PM1] Length = 376 Score = 659 bits (1700), Expect = 0.0, Method: Compositional matrix adjust. Identities = 312/375 (83%), Positives = 345/375 (92%) Query: 1 MSEKKPVIVYGASGYTGRLVCEFLREYNIPFIAAGRSAEKLNAAMKSNVAGIETADYEVV 60 MS KKPV+VYGASGYTGRL+CE+LREY IPFIAAGRSA+KL AM+SNV GIETA YE+ Sbjct: 1 MSNKKPVVVYGASGYTGRLICEYLREYGIPFIAAGRSADKLQTAMQSNVPGIETASYEIA 60 Query: 61 SVPHTVAALTELFKGASVVLNTVGPFAKFGGEVVQACLAARCHYTDTTGEQDWLITLEHE 120 VPH+VAALTELF+GASVVLNTVGPFAKFG EVV+ACLAARCHYTDTTGEQDWLITL+ + Sbjct: 61 EVPHSVAALTELFRGASVVLNTVGPFAKFGPEVVEACLAARCHYTDTTGEQDWLITLDEQ 120 Query: 121 FGTRFANAGLLLAPGIAQMYTTGEIAAQLCLETPGLDTLDIAVFWGGSPTIASTQTILVN 180 +G +FA AGLLL+PG+A MYTTGEIAAQLCLETPGLDTLDIAVFWGGSPTIASTQTILVN Sbjct: 121 YGAQFAAAGLLLSPGLAHMYTTGEIAAQLCLETPGLDTLDIAVFWGGSPTIASTQTILVN 180 Query: 181 AAMAKAYYLEQNKYVEHQPDAGLYNLAIPGQHELALALPWGGTSHPVWFKQDPRVANVKV 240 AA +KAYYL+QN+YVE QPDAGLYN+ IPGQHE ALALPWGGTSHPVWFK+DPRVA VKV Sbjct: 181 AATSKAYYLDQNRYVEWQPDAGLYNVTIPGQHEAALALPWGGTSHPVWFKRDPRVATVKV 240 Query: 241 LGGVFNRALMLGVPQIVAAALEATKDMNPDDRYAALAQTAAGVMNTMPPRENPRVNKSLD 300 LGGVFN+ LM GVP IVAAAL+AT+ MNP++RYAALAQTAAGVMNTMPPRENPR+NKS+D Sbjct: 241 LGGVFNKPLMQGVPLIVAAALKATEGMNPEERYAALAQTAAGVMNTMPPRENPRLNKSVD 300 Query: 301 SVYASGPLGRAHCVIYGNSNYKQTGMLQAYAAAHLLQQPPKRVGFASGCQAFGHRELLGQ 360 SV+ASGPL RAHCVI+GN NYKQTG+LQA+AAA LLQQ P+RVGFASGCQAFGH ELLG Sbjct: 301 SVHASGPLARAHCVIFGNCNYKQTGLLQAFAAASLLQQAPRRVGFASGCQAFGHHELLGA 360 Query: 361 LRSFGLVGKPVLTVH 375 LRSFGLV P+LTVH Sbjct: 361 LRSFGLVQAPILTVH 375 >ref|YP_004235456.1| Saccharopine dehydrogenase [Acidovorax avenae subsp. avenae ATCC 19860] gb|ADX46889.1| Saccharopine dehydrogenase [Acidovorax avenae subsp. avenae ATCC 19860] Length = 381 Score = 640 bits (1650), Expect = 0.0, Method: Compositional matrix adjust. Identities = 302/373 (80%), Positives = 340/373 (91%) Query: 4 KKPVIVYGASGYTGRLVCEFLREYNIPFIAAGRSAEKLNAAMKSNVAGIETADYEVVSVP 63 + PV+VYGASGYTGRLVCE+LREY IPF AAGRS EKL AAM+S+V GIETA Y++V VP Sbjct: 9 RHPVVVYGASGYTGRLVCEYLREYGIPFTAAGRSREKLEAAMRSHVPGIETATYDIVEVP 68 Query: 64 HTVAALTELFKGASVVLNTVGPFAKFGGEVVQACLAARCHYTDTTGEQDWLITLEHEFGT 123 H VA+LT LF+GASVVLNTVGPF KFG EVV+ACL+A CHYTDTTGEQDWLITL+ ++GT Sbjct: 69 HDVASLTALFQGASVVLNTVGPFTKFGPEVVEACLSAGCHYTDTTGEQDWLITLDEQYGT 128 Query: 124 RFANAGLLLAPGIAQMYTTGEIAAQLCLETPGLDTLDIAVFWGGSPTIASTQTILVNAAM 183 RFA+AGLLL+PG+AQMYTTGEIAAQL LETPGLDTLDIAVFWGGSPTIASTQTILVNAA Sbjct: 129 RFADAGLLLSPGLAQMYTTGEIAAQLALETPGLDTLDIAVFWGGSPTIASTQTILVNAAT 188 Query: 184 AKAYYLEQNKYVEHQPDAGLYNLAIPGQHELALALPWGGTSHPVWFKQDPRVANVKVLGG 243 AKA+YLEQN+Y E QPDAGLY LAIPGQHE+ALALPWGGTSHPVWF++DPRVANV+VLGG Sbjct: 189 AKAFYLEQNQYKEWQPDAGLYQLAIPGQHEVALALPWGGTSHPVWFRRDPRVANVRVLGG 248 Query: 244 VFNRALMLGVPQIVAAALEATKDMNPDDRYAALAQTAAGVMNTMPPRENPRVNKSLDSVY 303 VFN+ LMLGVP+IV AALEAT+DM+PD RY AL QTAAGVMNTMPPRENPR+NKS+DSV+ Sbjct: 249 VFNKPLMLGVPKIVEAALEATRDMDPDARYQALTQTAAGVMNTMPPRENPRINKSVDSVH 308 Query: 304 ASGPLGRAHCVIYGNSNYKQTGMLQAYAAAHLLQQPPKRVGFASGCQAFGHRELLGQLRS 363 ASGPLGRAHCVI+GN NYKQTG+LQA+AA+ LLQQPP+R GFASGCQAFGH LLG LR+ Sbjct: 309 ASGPLGRAHCVIHGNCNYKQTGLLQAFAASWLLQQPPRRTGFASGCQAFGHHALLGALRA 368 Query: 364 FGLVGKPVLTVHD 376 FGLV P+LTV + Sbjct: 369 FGLVQPPLLTVQN 381 >ref|ZP_00994246.1| Saccharopine dehydrogenase [Janibacter sp. HTCC2649] gb|EAQ00500.1| Saccharopine dehydrogenase [Janibacter sp. HTCC2649] Length = 375 Score = 599 bits (1544), Expect = e-169, Method: Compositional matrix adjust. Identities = 290/373 (77%), Positives = 323/373 (86%) Query: 4 KKPVIVYGASGYTGRLVCEFLREYNIPFIAAGRSAEKLNAAMKSNVAGIETADYEVVSVP 63 KPV+VYGASGYTGRLVCE+LREY +PF+AAGR+ ++LN +M S+VAGIETADYEV V Sbjct: 3 DKPVVVYGASGYTGRLVCEYLREYGVPFVAAGRNEDRLNESMSSHVAGIETADYEVTVVD 62 Query: 64 HTVAALTELFKGASVVLNTVGPFAKFGGEVVQACLAARCHYTDTTGEQDWLITLEHEFGT 123 H V ALTELFKGA VVLNTVGPF+K+G VV+A LAA HYTDTTGEQDWLIT + ++G Sbjct: 63 HDVEALTELFKGAKVVLNTVGPFSKWGPPVVEAALAAGVHYTDTTGEQDWLITCDEKWGE 122 Query: 124 RFANAGLLLAPGIAQMYTTGEIAAQLCLETPGLDTLDIAVFWGGSPTIASTQTILVNAAM 183 FA+ GLLL+PGIAQMYTTGEIAAQ+ LE PGLDTLDIAVFWGGSPTIASTQTILVNAA Sbjct: 123 AFADKGLLLSPGIAQMYTTGEIAAQVALEKPGLDTLDIAVFWGGSPTIASTQTILVNAAT 182 Query: 184 AKAYYLEQNKYVEHQPDAGLYNLAIPGQHELALALPWGGTSHPVWFKQDPRVANVKVLGG 243 + A+YLEQN+ V AGL L +PGQHELAL+LPWGGTSHP+WF++DPRVANVK GG Sbjct: 183 STAHYLEQNQLVAFDAAAGLVPLVVPGQHELALSLPWGGTSHPIWFRRDPRVANVKAQGG 242 Query: 244 VFNRALMLGVPQIVAAALEATKDMNPDDRYAALAQTAAGVMNTMPPRENPRVNKSLDSVY 303 VFN ALM GVPQIVAAALEATKDM+ +DR AAL TAA VMN MPPRENPRVNKSLDSV+ Sbjct: 243 VFNTALMHGVPQIVAAALEATKDMSEEDRDAALTATAAQVMNQMPPRENPRVNKSLDSVH 302 Query: 304 ASGPLGRAHCVIYGNSNYKQTGMLQAYAAAHLLQQPPKRVGFASGCQAFGHRELLGQLRS 363 ASGPLGRAHCVI+GNSNYKQTG+LQAYAA LLQ PPKRVGFASGCQAFGHRELLG LR+ Sbjct: 303 ASGPLGRAHCVIHGNSNYKQTGLLQAYAAYSLLQAPPKRVGFASGCQAFGHRELLGVLRT 362 Query: 364 FGLVGKPVLTVHD 376 FGLV +PVLTV D Sbjct: 363 FGLVSEPVLTVQD 375 >ref|ZP_08198753.1| hypothetical protein NBCG_03925 [Nocardioidaceae bacterium Broad-1] gb|EGD41737.1| hypothetical protein NBCG_03925 [Nocardioidaceae bacterium Broad-1] Length = 377 Score = 592 bits (1525), Expect = e-167, Method: Compositional matrix adjust. Identities = 281/373 (75%), Positives = 321/373 (86%) Query: 1 MSEKKPVIVYGASGYTGRLVCEFLREYNIPFIAAGRSAEKLNAAMKSNVAGIETADYEVV 60 MS+ KPV+VYGASGYTGRLVCE+LRE +PF+AAGRS +KL +M S+V G+ETADYE+ Sbjct: 1 MSDNKPVVVYGASGYTGRLVCEYLRELGVPFVAAGRSKDKLVDSMTSHVPGVETADYEIA 60 Query: 61 SVPHTVAALTELFKGASVVLNTVGPFAKFGGEVVQACLAARCHYTDTTGEQDWLITLEHE 120 V HTV ALTEL GA VVLNTVGPF+++G EVV+A LAA HYTDTTGEQDWLIT + + Sbjct: 61 EVEHTVEALTELLTGARVVLNTVGPFSQYGPEVVEAALAAGVHYTDTTGEQDWLITCDEK 120 Query: 121 FGTRFANAGLLLAPGIAQMYTTGEIAAQLCLETPGLDTLDIAVFWGGSPTIASTQTILVN 180 +G +A GLLLAPGIAQMYTTGEIAA++CLE PGLDTLDIAVFWGGSPTIAST+TILVN Sbjct: 121 YGADYAEKGLLLAPGIAQMYTTGEIAAEICLEQPGLDTLDIAVFWGGSPTIASTRTILVN 180 Query: 181 AAMAKAYYLEQNKYVEHQPDAGLYNLAIPGQHELALALPWGGTSHPVWFKQDPRVANVKV 240 AA +KA+YLEQN YVE P AGL L +PGQHELAL+LPWGGTSHPVW+++DP+VAN K Sbjct: 181 AATSKAHYLEQNAYVEFDPTAGLVPLVVPGQHELALSLPWGGTSHPVWYRRDPKVANCKA 240 Query: 241 LGGVFNRALMLGVPQIVAAALEATKDMNPDDRYAALAQTAAGVMNTMPPRENPRVNKSLD 300 GGVFN ALM GVPQIVA ALEATKDM+P+ R AL TAA VMN MPPRENPR+NKSLD Sbjct: 241 QGGVFNAALMNGVPQIVAGALEATKDMDPEQRDEALTATAAQVMNQMPPRENPRLNKSLD 300 Query: 301 SVYASGPLGRAHCVIYGNSNYKQTGMLQAYAAAHLLQQPPKRVGFASGCQAFGHRELLGQ 360 SV+ASGPLGRAHCVI+GN NYKQTG+LQAY A LLQQPP+RVGFASGCQAFGHRELLGQ Sbjct: 301 SVHASGPLGRAHCVIHGNQNYKQTGLLQAYVAYSLLQQPPRRVGFASGCQAFGHRELLGQ 360 Query: 361 LRSFGLVGKPVLT 373 L++FGLV +PV+T Sbjct: 361 LQAFGLVSEPVVT 373 >ref|YP_700905.1| hypothetical protein RHA1_ro00915 [Rhodococcus jostii RHA1] gb|ABG92747.1| conserved hypothetical protein [Rhodococcus jostii RHA1] Length = 373 Score = 508 bits (1308), Expect = e-142, Method: Compositional matrix adjust. Identities = 249/375 (66%), Positives = 288/375 (76%), Gaps = 3/375 (0%) Query: 1 MSEKKPVIVYGASGYTGRLVCEFLREYNIPFIAAGRSAEKLNAAMKSNVAGIETADYEVV 60 M++KKPVIVYG SGYTGRLVCE+LRE+NIPFIAAGR +++ + + G++T D+EVV Sbjct: 1 MTDKKPVIVYGVSGYTGRLVCEYLREFNIPFIAAGRDKKRIQEVL-DRIPGLDTVDHEVV 59 Query: 61 SVPHTVAALTELFKGASVVLNTVGPFAKFGGEVVQACLAARCHYTDTTGEQDWLITLEHE 120 V HTV ALTELF GA VV N VGPF K G VV+A LAA CHYTDTTGEQDW++ ++ + Sbjct: 60 EVEHTVPALTELFSGARVVSNMVGPFIKHGAIVVEAALAAGCHYTDTTGEQDWVLDVQAK 119 Query: 121 FGTRFANAGLLLAPGIAQMYTTGEIAAQLCLETPGLDTLDIAVFWGGSPTIASTQTILVN 180 FG +FA GLLL+PG+AQMYTTGEIAA + LETPGLDTLDI V W G PT ASTQTI Sbjct: 120 FGEKFAEKGLLLSPGVAQMYTTGEIAANIALETPGLDTLDILVLWKGFPTYASTQTIF-T 178 Query: 181 AAMAKAYYLEQNKYVEHQPDAGLYNLAIPGQHELALALPWGGTSHPVWFKQDPRVANVKV 240 A YYLEQN+Y + P + + +PGQH++ALALPWGGTSHPVWFK DPRVANVK Sbjct: 179 ILKADWYYLEQNRYEKWDPGT-TFEVVVPGQHQMALALPWGGTSHPVWFKDDPRVANVKA 237 Query: 241 LGGVFNRALMLGVPQIVAAALEATKDMNPDDRYAALAQTAAGVMNTMPPRENPRVNKSLD 300 GGVF+R++M GV E K + D++ AALA+ A V MPPRENPRVN SLD Sbjct: 238 AGGVFDRSVMEGVVATQKMVEEQIKTLPADEQDAALAEIAGSVQAGMPPRENPRVNTSLD 297 Query: 301 SVYASGPLGRAHCVIYGNSNYKQTGMLQAYAAAHLLQQPPKRVGFASGCQAFGHRELLGQ 360 SVYASGPLGRAHCVI+GN NYKQTG+LQAYAA LLQQPPKR G AS CQAFGHRELLG Sbjct: 298 SVYASGPLGRAHCVIHGNCNYKQTGLLQAYAAYSLLQQPPKRTGLASACQAFGHRELLGI 357 Query: 361 LRSFGLVGKPVLTVH 375 LRSFGLV +PVLTV Sbjct: 358 LRSFGLVMEPVLTVQ 372