BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0190.p (509 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_001019374.1| putative ligase [Methylibium petroleiphilum ... 766 0.0 ref|YP_002777841.1| fatty-acid--CoA ligase [Rhodococcus opacus B... 748 0.0 ref|YP_700904.1| AMP-dependent acyl-CoA synthetase [Rhodococcus ... 740 0.0 ref|YP_002768605.1| fatty-acid--CoA ligase [Rhodococcus erythrop... 733 0.0 ref|ZP_04382834.1| AMP-dependent synthetase and ligase [Rhodococ... 732 0.0 >ref|YP_001019374.1| putative ligase [Methylibium petroleiphilum PM1] gb|ABM93139.1| putative ligase [Methylibium petroleiphilum PM1] Length = 514 Score = 766 bits (1978), Expect = 0.0, Method: Compositional matrix adjust. Identities = 370/508 (72%), Positives = 419/508 (82%), Gaps = 1/508 (0%) Query: 1 MRFIDYLDKGASLGADAPCLTMDGRDLSYGEVQRLSYRIARGLERSGIAPGEKVAILSGN 60 MR +DYLDKGA LGADAPCLTM DLSY +VQR+SYR+ARGL+RSGIAPG+KVA+LS N Sbjct: 1 MRLVDYLDKGAQLGADAPCLTMGDADLSYADVQRISYRVARGLQRSGIAPGDKVAVLSSN 60 Query: 61 DPLAFACVFGISRAAAVWCPINPRNEAAENKFILDQFDCSLLLFHSSFAPMVDAVRQDLP 120 LAFACVFGI+RA AVWCPINPRNEA+EN+++LD FDC+ ++FHS++APMV+ +R LP Sbjct: 61 HALAFACVFGIARAGAVWCPINPRNEASENRYVLDAFDCACVIFHSNYAPMVEQMRAGLP 120 Query: 121 KLRTLVCLDAELPFAPSFERWLGGLTDEVYQREAIDDLAMIPGTGGTTGKPKGVMLSGRN 180 KLR LVCLD PFA F WL GL DE E DDLAMI GTGGTTG PKGVML+GRN Sbjct: 121 KLRLLVCLDQPAPFAAGFGDWLHGLGDEPLHVEPPDDLAMIAGTGGTTGSPKGVMLTGRN 180 Query: 181 IEAMTALTLMGYPFKGRPIYLALAPLTHAAGVLCFPIMTLGGRVVIMHHPDIGEFLALIE 240 +E M+ALTLMGYPF GRP YLALAPLTHAAGVLC P+M LGGRVVIM PD+GEFLALIE Sbjct: 181 LETMSALTLMGYPFDGRPSYLALAPLTHAAGVLCLPVMALGGRVVIMPKPDLGEFLALIE 240 Query: 241 RYRVTHTFLPPTVIYMLLDHPGLDAADRSSLQCFWYGAAPISATRLAEALKRIGP-MAQL 299 R+R+THTFLPPT+IYMLL H L A SLQCFWYGAAPISA RL EAL +IGP MAQL Sbjct: 241 RHRITHTFLPPTLIYMLLQHERLATARLDSLQCFWYGAAPISAARLEEALTKIGPVMAQL 300 Query: 300 FGQTEAPMMISMMPPADHYNPDGTIAMERLSSAGRIGPLVQAGIMDGDGKLLPAGSRGEI 359 FGQTEAPMMISMM P +H+ DG +A RLSSAGR GPLVQ MDG+G+LLP G GEI Sbjct: 301 FGQTEAPMMISMMSPREHFQTDGAVARARLSSAGRPGPLVQVATMDGEGRLLPTGETGEI 360 Query: 360 VVRGSLVMEGYYKNPEATREASAHGWHHTGDIGYIDGDGYLYIVDRAKDMIITGGFNVYS 419 VVRGSLVM GYYK+P+AT EA HGWHHTGDIG +D DG+L+IVDRAKDMIITGGFNVYS Sbjct: 361 VVRGSLVMAGYYKDPKATAEAGRHGWHHTGDIGRLDADGFLFIVDRAKDMIITGGFNVYS 420 Query: 420 IEVENALRAHEAVQDCAVIGLPDDKWGERIVAVVQPRAGHTVEATALAAFVKQQIGSVKT 479 +EVE AL H VQD AVIGLPDDKWGER+VAV+Q AG +V+ + AFVK +IGSVK+ Sbjct: 421 VEVEQALMQHPDVQDSAVIGLPDDKWGERVVAVLQLHAGRSVKPEDIQAFVKARIGSVKS 480 Query: 480 PKQIEVWDDLPRSKVGKVLKPDIRARLV 507 PKQ+E+W DLPRSKVGKVLK DIRA L+ Sbjct: 481 PKQVEIWSDLPRSKVGKVLKKDIRASLL 508 >ref|YP_002777841.1| fatty-acid--CoA ligase [Rhodococcus opacus B4] dbj|BAH48896.1| putative fatty-acid--CoA ligase [Rhodococcus opacus B4] Length = 506 Score = 748 bits (1930), Expect = 0.0, Method: Compositional matrix adjust. Identities = 364/508 (71%), Positives = 416/508 (81%), Gaps = 4/508 (0%) Query: 1 MRFIDYLDKGASLGADAPCLTMDGRDLSYGEVQRLSYRIARGLERSGIAPGEKVAILSGN 60 MR DYLDKGASLG +PCLTM R LSY +VQ LS R+A L RSGIA G+KVA+LSGN Sbjct: 1 MRLTDYLDKGASLGPSSPCLTMGERTLSYADVQDLSRRVAAALARSGIAAGDKVAVLSGN 60 Query: 61 DPLAFACVFGISRAAAVWCPINPRNEAAENKFILDQFDCSLLLFHSSFAPMVDAVRQDLP 120 DP AF+CVFGISRA AVWCPINPRNEAAEN+ +LD FDC L+F +FAP+VD +R+ LP Sbjct: 61 DPTAFSCVFGISRAGAVWCPINPRNEAAENRDLLDLFDCRCLIFQKAFAPLVDRIREQLP 120 Query: 121 KLRTLVCLDAELPFAPSFERWLGGLTDEVYQREAIDDLAMIPGTGGTTGKPKGVMLSGRN 180 L TLVCLD + +A SF+ WLG E +DDLA+I GTGGTTG+PKGVML+GRN Sbjct: 121 LLTTLVCLDGDETYAESFDTWLGAAR---LDAEPVDDLALIVGTGGTTGRPKGVMLTGRN 177 Query: 181 IEAMTALTLMGYPFKGRPIYLALAPLTHAAGVLCFPIMTLGGRVVIMHHPDIGEFLALIE 240 IE M+ALTLM YPF GRP+YLALAPLTHAAGVLCFPI+TLGG+VVIM PD+G+FLA I+ Sbjct: 178 IETMSALTLMSYPFDGRPVYLALAPLTHAAGVLCFPILTLGGQVVIMPAPDLGQFLAHID 237 Query: 241 RYRVTHTFLPPTVIYMLLDHPGLDAADRSSLQCFWYGAAPISATRLAEALKRIGP-MAQL 299 R RVTHTFLPPT+IYMLLDH L+ D SLQCFWYGAAPIS TRL+EAL RIGP MAQL Sbjct: 238 RDRVTHTFLPPTLIYMLLDHADLETTDLGSLQCFWYGAAPISPTRLSEALTRIGPVMAQL 297 Query: 300 FGQTEAPMMISMMPPADHYNPDGTIAMERLSSAGRIGPLVQAGIMDGDGKLLPAGSRGEI 359 FGQ+EAPMMI+ M P DH++ DG++A RLSSAGR PLV IMDG G LLPAG RGE+ Sbjct: 298 FGQSEAPMMIATMAPKDHFHEDGSVATARLSSAGRPSPLVTVAIMDGQGILLPAGERGEV 357 Query: 360 VVRGSLVMEGYYKNPEATREASAHGWHHTGDIGYIDGDGYLYIVDRAKDMIITGGFNVYS 419 VVR SLVM GYYKNP AT+EASAHGWHHTGDIGY+D D +LYIVDRAKDMIITGGFNVYS Sbjct: 358 VVRSSLVMAGYYKNPVATQEASAHGWHHTGDIGYLDEDNFLYIVDRAKDMIITGGFNVYS 417 Query: 420 IEVENALRAHEAVQDCAVIGLPDDKWGERIVAVVQPRAGHTVEATALAAFVKQQIGSVKT 479 +EVE AL A+ +QDC VIGLPD+KWGER+VAVVQ RAG +EA AL AFVK +IGSVKT Sbjct: 418 VEVEQALMAYPGIQDCGVIGLPDEKWGERVVAVVQARAGTPIEADALTAFVKARIGSVKT 477 Query: 480 PKQIEVWDDLPRSKVGKVLKPDIRARLV 507 PK+I VW DLPRSK+GKVLK +IR +L+ Sbjct: 478 PKEILVWPDLPRSKLGKVLKNEIRQQLL 505 >ref|YP_700904.1| AMP-dependent acyl-CoA synthetase [Rhodococcus jostii RHA1] gb|ABG92746.1| probable AMP-dependent acyl-CoA synthetase [Rhodococcus jostii RHA1] Length = 506 Score = 740 bits (1910), Expect = 0.0, Method: Compositional matrix adjust. Identities = 362/508 (71%), Positives = 413/508 (81%), Gaps = 4/508 (0%) Query: 1 MRFIDYLDKGASLGADAPCLTMDGRDLSYGEVQRLSYRIARGLERSGIAPGEKVAILSGN 60 MR DYLDKGASLG +PCLTM R LSY +VQ LS R+A L RSGIA G+KVA+LSGN Sbjct: 1 MRLTDYLDKGASLGPSSPCLTMGDRTLSYADVQDLSRRVAAALARSGIAAGDKVAVLSGN 60 Query: 61 DPLAFACVFGISRAAAVWCPINPRNEAAENKFILDQFDCSLLLFHSSFAPMVDAVRQDLP 120 DP AF+CVFGISRA AVWCPINPRNEAAEN+ +L FDC L+F +FAP+VD +R+ LP Sbjct: 61 DPTAFSCVFGISRAGAVWCPINPRNEAAENRDLLALFDCRCLIFQKAFAPLVDRIRRQLP 120 Query: 121 KLRTLVCLDAELPFAPSFERWLGGLTDEVYQREAIDDLAMIPGTGGTTGKPKGVMLSGRN 180 L TLVCLD + +A F+ WLG E E +DDLA+I GTGGTTG+PKGVML+GRN Sbjct: 121 LLTTLVCLDGDEEYAHPFDDWLG---TERLDVEPVDDLALIVGTGGTTGRPKGVMLTGRN 177 Query: 181 IEAMTALTLMGYPFKGRPIYLALAPLTHAAGVLCFPIMTLGGRVVIMHHPDIGEFLALIE 240 IE M+ALTLM YPF GRP+YLALAPLTHAAGVLCFPI+ LGG+VVIM PD+G+FLA I+ Sbjct: 178 IETMSALTLMSYPFDGRPVYLALAPLTHAAGVLCFPILALGGQVVIMPTPDLGQFLAHID 237 Query: 241 RYRVTHTFLPPTVIYMLLDHPGLDAADRSSLQCFWYGAAPISATRLAEALKRIGP-MAQL 299 R RVTHTFLPPT+IYMLLDH L D SSLQCFWYGAAPIS TRL EAL RIGP MAQL Sbjct: 238 RDRVTHTFLPPTLIYMLLDHADLATTDLSSLQCFWYGAAPISPTRLEEALTRIGPVMAQL 297 Query: 300 FGQTEAPMMISMMPPADHYNPDGTIAMERLSSAGRIGPLVQAGIMDGDGKLLPAGSRGEI 359 FGQ+EAPMMI+ M P DH++ DG++A RLSSAGR PLV IMDG G LLP+G RGE+ Sbjct: 298 FGQSEAPMMIATMAPKDHFHADGSVATARLSSAGRPSPLVTVAIMDGQGTLLPSGERGEV 357 Query: 360 VVRGSLVMEGYYKNPEATREASAHGWHHTGDIGYIDGDGYLYIVDRAKDMIITGGFNVYS 419 VVR SLVM GYYKNP AT+EASAHGWHHTGDIGY+D D +LYIVDRAKDMIITGGFNVYS Sbjct: 358 VVRSSLVMTGYYKNPVATQEASAHGWHHTGDIGYLDDDNFLYIVDRAKDMIITGGFNVYS 417 Query: 420 IEVENALRAHEAVQDCAVIGLPDDKWGERIVAVVQPRAGHTVEATALAAFVKQQIGSVKT 479 +EVE AL A+ +QDC VIGLPD+KWGER+VAVVQ RAG +EA AL AFVK +IGSVKT Sbjct: 418 VEVEQALMAYPGIQDCGVIGLPDEKWGERVVAVVQSRAGTPIEADALTAFVKARIGSVKT 477 Query: 480 PKQIEVWDDLPRSKVGKVLKPDIRARLV 507 PK+I VW DLPRSK+GKVLK +IR +L+ Sbjct: 478 PKEILVWPDLPRSKLGKVLKNEIRQQLL 505 >ref|YP_002768605.1| fatty-acid--CoA ligase [Rhodococcus erythropolis PR4] dbj|BAH35866.1| putative fatty-acid--CoA ligase [Rhodococcus erythropolis PR4] Length = 506 Score = 733 bits (1892), Expect = 0.0, Method: Compositional matrix adjust. Identities = 353/508 (69%), Positives = 406/508 (79%), Gaps = 4/508 (0%) Query: 1 MRFIDYLDKGASLGADAPCLTMDGRDLSYGEVQRLSYRIARGLERSGIAPGEKVAILSGN 60 MR DYLDKGASLG+ APCLTM R LSY EVQ+ S R A L SGIA GEKVAILSGN Sbjct: 1 MRLTDYLDKGASLGSTAPCLTMGERTLSYSEVQQFSRRFAASLAASGIAAGEKVAILSGN 60 Query: 61 DPLAFACVFGISRAAAVWCPINPRNEAAENKFILDQFDCSLLLFHSSFAPMVDAVRQDLP 120 DP++F+CVFGISRA AVWCPINPRNEAAEN+ +LD FDC L F S+AP+VD ++ LP Sbjct: 61 DPISFSCVFGISRAGAVWCPINPRNEAAENRDLLDLFDCRALFFQKSYAPLVDQIKDALP 120 Query: 121 KLRTLVCLDAELPFAPSFERWLGGLTDEVYQREAIDDLAMIPGTGGTTGKPKGVMLSGRN 180 +L TLVC+D E A S E WLG +E A DD+ M+ GTGGTTG+PKGVML+G N Sbjct: 121 QLVTLVCIDGEWDSAQSLESWLG---EEELDVAAPDDVTMLVGTGGTTGRPKGVMLTGHN 177 Query: 181 IEAMTALTLMGYPFKGRPIYLALAPLTHAAGVLCFPIMTLGGRVVIMHHPDIGEFLALIE 240 IE MTA+TLM YPF+GRP+YLALAPLTHAAGVLCFPIM LGG VVIM PD+G FLA ++ Sbjct: 178 IETMTAITLMSYPFEGRPVYLALAPLTHAAGVLCFPIMALGGEVVIMPKPDLGYFLANVQ 237 Query: 241 RYRVTHTFLPPTVIYMLLDHPGLDAADRSSLQCFWYGAAPISATRLAEALKRIGP-MAQL 299 +++VTHTFLPPT+IYMLL+H L DRSSLQCFWYGAAPIS TRL EA+ +IGP MAQL Sbjct: 238 KHKVTHTFLPPTLIYMLLEHEDLAVTDRSSLQCFWYGAAPISPTRLEEAIDKIGPVMAQL 297 Query: 300 FGQTEAPMMISMMPPADHYNPDGTIAMERLSSAGRIGPLVQAGIMDGDGKLLPAGSRGEI 359 FGQ+EAPMMIS M P +H+ PDGT+A RLSSAGR PLV GIMD G LL G RGE+ Sbjct: 298 FGQSEAPMMISTMSPKEHFGPDGTVATSRLSSAGRPSPLVTVGIMDDKGALLGRGERGEV 357 Query: 360 VVRGSLVMEGYYKNPEATREASAHGWHHTGDIGYIDGDGYLYIVDRAKDMIITGGFNVYS 419 V+R SLVM GYYKNP+AT EASAHGWHHTGDIGY+D D +L+IVDRAKDMIITGGFNVYS Sbjct: 358 VIRSSLVMAGYYKNPDATAEASAHGWHHTGDIGYLDEDNFLFIVDRAKDMIITGGFNVYS 417 Query: 420 IEVENALRAHEAVQDCAVIGLPDDKWGERIVAVVQPRAGHTVEATALAAFVKQQIGSVKT 479 +EVE AL A+ +QDC VIGLPDDKWGER+VAVVQP G ++ + AFVK +IGSVKT Sbjct: 418 VEVEQALMAYPGIQDCGVIGLPDDKWGERVVAVVQPHLGADIDGAEVMAFVKSRIGSVKT 477 Query: 480 PKQIEVWDDLPRSKVGKVLKPDIRARLV 507 PKQ+ VWDDLPRSKVGKVLK +I++ L Sbjct: 478 PKQVIVWDDLPRSKVGKVLKNEIKSELT 505 >ref|ZP_04382834.1| AMP-dependent synthetase and ligase [Rhodococcus erythropolis SK121] gb|EEN90227.1| AMP-dependent synthetase and ligase [Rhodococcus erythropolis SK121] Length = 506 Score = 732 bits (1889), Expect = 0.0, Method: Compositional matrix adjust. Identities = 354/508 (69%), Positives = 407/508 (80%), Gaps = 4/508 (0%) Query: 1 MRFIDYLDKGASLGADAPCLTMDGRDLSYGEVQRLSYRIARGLERSGIAPGEKVAILSGN 60 MR DYLDKGASLG+ APCLTM R LSYGEVQ+ S R A L S IAPGEKVAILSGN Sbjct: 1 MRLTDYLDKGASLGSTAPCLTMGERTLSYGEVQQFSRRFAASLAASDIAPGEKVAILSGN 60 Query: 61 DPLAFACVFGISRAAAVWCPINPRNEAAENKFILDQFDCSLLLFHSSFAPMVDAVRQDLP 120 DP++F+CVFGISRA VWCPINPRNEAAEN+ +LD FDC L F S+AP+VD ++ LP Sbjct: 61 DPISFSCVFGISRAGGVWCPINPRNEAAENRDLLDLFDCRALFFQKSYAPLVDQIKDALP 120 Query: 121 KLRTLVCLDAELPFAPSFERWLGGLTDEVYQREAIDDLAMIPGTGGTTGKPKGVMLSGRN 180 +L TLVC+D E A S E WLG DE+ A DD+ M+ GTGGTTG+PKGVML+G N Sbjct: 121 QLVTLVCIDGESESAQSLESWLG--EDEL-DVAAPDDVTMLVGTGGTTGRPKGVMLTGHN 177 Query: 181 IEAMTALTLMGYPFKGRPIYLALAPLTHAAGVLCFPIMTLGGRVVIMHHPDIGEFLALIE 240 IE MTA+TLM YPF+GRP+YLALAPLTHAAGVLCFPIM LGG VVIM PD+G FLA ++ Sbjct: 178 IETMTAITLMSYPFEGRPVYLALAPLTHAAGVLCFPIMALGGEVVIMPKPDLGYFLANVQ 237 Query: 241 RYRVTHTFLPPTVIYMLLDHPGLDAADRSSLQCFWYGAAPISATRLAEALKRIGP-MAQL 299 +++VTHTFLPPT+IYMLL+H L A D SSLQCFWYGAAPIS TRLAEA+ +IGP MAQL Sbjct: 238 KHKVTHTFLPPTLIYMLLEHEALSATDLSSLQCFWYGAAPISPTRLAEAIDKIGPVMAQL 297 Query: 300 FGQTEAPMMISMMPPADHYNPDGTIAMERLSSAGRIGPLVQAGIMDGDGKLLPAGSRGEI 359 FGQ+EAPMMIS M P +H+ DGT+A RLSSAGR PLV GIMD G LL G RGE+ Sbjct: 298 FGQSEAPMMISTMSPKEHFGSDGTVATSRLSSAGRPSPLVTVGIMDDKGALLGHGERGEV 357 Query: 360 VVRGSLVMEGYYKNPEATREASAHGWHHTGDIGYIDGDGYLYIVDRAKDMIITGGFNVYS 419 V+R SLVM GYYKNP+AT EASAHGWHHTGDIGY+D D +L+IVDRAKDMIITGGFNVYS Sbjct: 358 VIRSSLVMAGYYKNPDATAEASAHGWHHTGDIGYLDDDNFLFIVDRAKDMIITGGFNVYS 417 Query: 420 IEVENALRAHEAVQDCAVIGLPDDKWGERIVAVVQPRAGHTVEATALAAFVKQQIGSVKT 479 +EVE AL A+ +QDC VIGLPDDKWGER+VAVVQP G ++ + AFVK +IGSVKT Sbjct: 418 VEVEQALMAYPGIQDCGVIGLPDDKWGERVVAVVQPHLGADIDGAEVMAFVKARIGSVKT 477 Query: 480 PKQIEVWDDLPRSKVGKVLKPDIRARLV 507 PKQ+ VWDDLPRSKVGKVLK +I++ L Sbjct: 478 PKQVIVWDDLPRSKVGKVLKNEIKSELT 505