BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0151.p (265 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_770829.1| acetyl-hydrolase [Bradyrhizobium japonicum USDA... 390 e-106 ref|ZP_08198514.1| lipase/esterase [Nocardioidaceae bacterium Br... 236 2e-60 gb|ADR31550.1| EST1 [uncultured microorganism] 214 1e-53 gb|ACL67843.1| lipolytic enzyme [uncultured bacterium] 212 5e-53 ref|YP_004170539.1| alpha/beta hydrolase fold-3 [Deinococcus mar... 211 1e-52 >ref|NP_770829.1| acetyl-hydrolase [Bradyrhizobium japonicum USDA 110] dbj|BAC49454.1| bll4189 [Bradyrhizobium japonicum USDA 110] Length = 325 Score = 390 bits (1001), Expect = e-106, Method: Compositional matrix adjust. Identities = 209/264 (79%), Positives = 229/264 (86%) Query: 1 VQGAAGKLGARLYRNGRGTAPTVIFFHGGGWVAGDLETHDRQARNLVIDTGAVVISVDYR 60 V+G AG L ARLYR G APTV+FFHGGGWVAGDLETHDRQARNL I+TGAVV+SVDYR Sbjct: 61 VKGGAGPLDARLYRIGSDPAPTVVFFHGGGWVAGDLETHDRQARNLAIETGAVVVSVDYR 120 Query: 61 QPPETRFPGAFEDAFAAIRDVVTRIGEFGGDLARIGVAGDSAGGNLAATTALACRDASIR 120 +PPETRFPGAFEDAFAA RD+ R+ EFGGD R+GVAGDSAGGNLAA TA+ACRDA I+ Sbjct: 121 RPPETRFPGAFEDAFAAARDIFNRVAEFGGDAKRLGVAGDSAGGNLAAATAIACRDAGIK 180 Query: 121 LAGQLLVYPVTDVAGNFADAAENAKFPSRAENADGYFLTRATMQWFCGHYLADRAEGTDW 180 LA QLLVYPVTDVAG++ADA ENA+FPSRAENADGYFL+RA M+WFCGHYLAD G DW Sbjct: 181 LAAQLLVYPVTDVAGHYADARENARFPSRAENADGYFLSRAVMEWFCGHYLADPGHGADW 240 Query: 181 RVSPLRAKSLKGVAPAVVTTAWFDPLRDEGHAYAKALQAAGVPVAYHSGEGLIHGYFGLG 240 RVSPLRA SL G+APA+V TA FDPLRDEG AYA+AL+AAGV V +H G GLIHGYFGLG Sbjct: 241 RVSPLRAASLAGLAPAIVATASFDPLRDEGAAYARALEAAGVRVKHHEGPGLIHGYFGLG 300 Query: 241 DASDAARAEAQRARADFKVLLERG 264 DASD ARAEAQRARADFK LL RG Sbjct: 301 DASDVARAEAQRARADFKALLARG 324 >ref|ZP_08198514.1| lipase/esterase [Nocardioidaceae bacterium Broad-1] gb|EGD42000.1| lipase/esterase [Nocardioidaceae bacterium Broad-1] Length = 313 Score = 236 bits (603), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 136/263 (51%), Positives = 172/263 (65%), Gaps = 13/263 (4%) Query: 1 VQGAAGKLGARLYR-NGRGTAPTVIFFHGGGWVAGDLETHDRQARNLVIDTGAVVISVDY 59 + G AG L R+YR +GT PT++ FHGGGWV GDL+TH+ QARNL AVV++VDY Sbjct: 62 IPGPAGDLAVRIYRPTTQGTLPTIVMFHGGGWVVGDLDTHEDQARNLSTICDAVVVAVDY 121 Query: 60 RQPPETRFPGAFEDAFAAIRDVVTRIGEFGGDLARIGVAGDSAGGNLAATTALACRDASI 119 R PE FP A++DA AA R V GGD R+ VAGDSAGGNL+A A A R Sbjct: 122 RLAPEHPFPAAYDDALAAARWVSDNTATLGGD-GRVAVAGDSAGGNLSAAVAQAFRAEGR 180 Query: 120 RLAGQLLVYPVTDVAGNFADAAENAKFPSRAENADGYFLTRATMQWFCGHYLADRAEGTD 179 LAGQLL+YP TD+ + +PS AEN GYFL TM+WF +Y+ D A+ D Sbjct: 181 ELAGQLLIYPATDM---------TSGYPSYAENGHGYFLELPTMKWFSDNYVGD-ADRAD 230 Query: 180 WRVSPLRAKSLKGVAPAVVTTAWFDPLRDEGHAYAKALQAAGVPVAYHSGEGLIHGYFGL 239 R+SP++ SL+G+APAVV TA FDPLRDEG AYAKAL+++GVP YH +GLIHG+F + Sbjct: 231 PRLSPIKG-SLEGLAPAVVVTAEFDPLRDEGIAYAKALESSGVPTTYHHCDGLIHGFFAM 289 Query: 240 GDASDAARAEAQRARADFKVLLE 262 G S AA+ + A F+ +L Sbjct: 290 GHHSQAAQLAIEETCALFRTVLH 312 >gb|ADR31550.1| EST1 [uncultured microorganism] Length = 311 Score = 214 bits (544), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 124/263 (47%), Positives = 151/263 (57%), Gaps = 15/263 (5%) Query: 1 VQGAAGKLGARLYRN-GRGTAPTVIFFHGGGWVAGDLETHDRQARNLVIDTGAVVISVDY 59 + G AG++ R+Y G G P ++FFHGGGWV DL+THD R+L G VV+SVDY Sbjct: 56 IPGPAGEIPIRIYTPAGSGPFPALVFFHGGGWVICDLDTHDSLCRSLCNGAGCVVVSVDY 115 Query: 60 RQPPETRFPGAFEDAFAAIRDVVTRIGEFGGDLARIGVAGDSAGGNLAATTALACRDAS- 118 R PE +FP A ED +AA + V E D I V GDSAGGNL A A RD Sbjct: 116 RLAPEHKFPAAPEDCYAATQWVAGHAAEINADPDSIAVGGDSAGGNLTAVVAQMARDQDG 175 Query: 119 IRLAGQLLVYPVTDVAGNFADAAENAKFPSRAENADGYFLTRATMQWFCGHYLADRAEGT 178 LA QLL+YP TD + PS ENA+GYFLT M WF HYL A+ T Sbjct: 176 PALAFQLLIYPATDFTFD---------GPSLRENAEGYFLTSDDMDWFTNHYLNSNADRT 226 Query: 179 DWRVSPLRAKSLKGVAPAVVTTAWFDPLRDEGHAYAKALQAAGVPVAYHSGEGLIHGYFG 238 + SP++A L + PA+V TA +DPLRDEG +Y K LQ AGVPV +G+IHG+ Sbjct: 227 NPLASPMQADDLSELPPALVITAEYDPLRDEGESYGKQLQEAGVPVTISRYDGMIHGFLS 286 Query: 239 LGDASDA---ARAE-AQRARADF 257 L +D ARAE AQ RA F Sbjct: 287 LEPMTDKGQQARAECAQALRAAF 309 >gb|ACL67843.1| lipolytic enzyme [uncultured bacterium] Length = 311 Score = 212 bits (539), Expect = 5e-53, Method: Compositional matrix adjust. Identities = 115/241 (47%), Positives = 142/241 (58%), Gaps = 11/241 (4%) Query: 1 VQGAAGKLGARLYR-NGRGTAPTVIFFHGGGWVAGDLETHDRQARNLVIDTGAVVISVDY 59 + G AG++ R+Y G P ++FFHGGG+V GDLETHD + R L +V+SVDY Sbjct: 54 IPGPAGEIPIRVYTPKGDTPLPVLVFFHGGGFVIGDLETHDAECRALANAADCIVVSVDY 113 Query: 60 RQPPETRFPGAFEDAFAAIRDVVTRIGEFGGDLARIGVAGDSAGGNLAATTALACRD-AS 118 R PE +FP A +DAFAA V + G D RI V GDSAGG+LA + +D Sbjct: 114 RLAPEHKFPAALDDAFAATEWVASNASAIGADPNRIAVGGDSAGGSLATVVSQMAKDRGG 173 Query: 119 IRLAGQLLVYPVTDVAGNFADAAENAKFPSRAENADGYFLTRATMQWFCGHYLADRAEGT 178 RLA QLLVYP T + A S AENADGYFLTR M WF Y +G+ Sbjct: 174 PRLAFQLLVYPPTQYGFDTA---------SHAENADGYFLTRDMMDWFLAQYFTGEVDGS 224 Query: 179 DWRVSPLRAKSLKGVAPAVVTTAWFDPLRDEGHAYAKALQAAGVPVAYHSGEGLIHGYFG 238 D R+SPLR L G+ PA+V TA FDPLRD+G AYA L AGVP +G+IHG+F Sbjct: 225 DPRISPLRTADLSGLPPALVITAEFDPLRDDGEAYAARLAEAGVPAKNTRYDGMIHGFFS 284 Query: 239 L 239 + Sbjct: 285 M 285 >ref|YP_004170539.1| alpha/beta hydrolase fold-3 [Deinococcus maricopensis DSM 21211] gb|ADV66874.1| alpha/beta hydrolase fold-3 [Deinococcus maricopensis DSM 21211] Length = 315 Score = 211 bits (536), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 122/241 (50%), Positives = 147/241 (60%), Gaps = 10/241 (4%) Query: 1 VQGAAGKLGARLYRN-GRGTAPTVIFFHGGGWVAGDLETHDRQARNLVIDTGAVVISVDY 59 + G G L AR Y G G P V+FFHGGG+VA D++THD R L GA+V+SV Y Sbjct: 55 IPGPGGALPARAYTPAGDGPFPVVVFFHGGGFVAYDIDTHDHVCRELCQGAGALVVSVAY 114 Query: 60 RQPPETRFPGAFEDAFAAIRDVVTRIGEFGGDLARIGVAGDSAGGNLAATTALACRD-AS 118 R PE +FP A +DA AA+R V + GGD AR+GVAGDSAG NLA TAL RD Sbjct: 115 RLAPEHKFPAATDDALAAVRWVGDHARDLGGDPARLGVAGDSAGANLATVTALRVRDEGG 174 Query: 119 IRLAGQLLVYPVTDVAGNFADAAENAKFPSRAENADGYFLTRATMQWFCGHYLADRAEGT 178 RL+ QLL+YP D+A + PS ENA+GYFLT ++ F YL + Sbjct: 175 PRLSAQLLIYPAVDMA--------DETSPSMRENANGYFLTEERLRSFGDAYLRTPDDAR 226 Query: 179 DWRVSPLRAKSLKGVAPAVVTTAWFDPLRDEGHAYAKALQAAGVPVAYHSGEGLIHGYFG 238 SPLRA SL G+ PA++ TA FDPLRD+G AYA AL AAGVP Y G GLIHG+ Sbjct: 227 HPHASPLRAPSLHGLPPALIVTAEFDPLRDQGRAYADALNAAGVPARYLPGPGLIHGFAN 286 Query: 239 L 239 L Sbjct: 287 L 287