BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0137.p (451 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_772512.1| glucarate dehydratase [Bradyrhizobium japonicum... 814 0.0 ref|YP_001242303.1| (D)-glucarate dehydratase 1 [Bradyrhizobium ... 806 0.0 ref|YP_001203683.1| (D)-glucarate dehydratase 1 [Bradyrhizobium ... 803 0.0 ref|YP_002943647.1| glucarate dehydratase [Variovorax paradoxus ... 707 0.0 ref|YP_002502139.1| glucarate dehydratase [Methylobacterium nodu... 701 0.0 >ref|NP_772512.1| glucarate dehydratase [Bradyrhizobium japonicum USDA 110] dbj|BAC51137.1| glucarate dehydratase [Bradyrhizobium japonicum USDA 110] Length = 452 Score = 814 bits (2102), Expect = 0.0, Method: Compositional matrix adjust. Identities = 391/441 (88%), Positives = 416/441 (94%) Query: 11 AGAPVVTAMEVIPVAGRDGMLLNLSGAHAPFFTRNLVILTDNSGHTGVGEVPGGEKIKKT 70 AGAPVVTAM+VIPVAGRD MLLNLSGAHAPFFTRN+VILTDN+GHTGVGEVPGG+KI +T Sbjct: 12 AGAPVVTAMQVIPVAGRDSMLLNLSGAHAPFFTRNIVILTDNAGHTGVGEVPGGQKIWQT 71 Query: 71 LEDARDLVVGQTIGAYNNILASMRQAFADRDADGRGKQTFDLRVTIHAVTAVESALLDLL 130 L+DARDLV+G+T+GA NNILA +R AFADRDA GRGKQTFDLRV IHAVTA+ESALLDLL Sbjct: 72 LQDARDLVIGKTVGAMNNILADVRTAFADRDAGGRGKQTFDLRVMIHAVTAIESALLDLL 131 Query: 131 GQHLNLPVAALLGEGQQRTSVETLGYLFFVGDRRKTSLPYVEGETGKPDWFNLRHQVAMT 190 GQHLNLPVAALLGEGQQR SVETLGYLFFVGD K+ L YV GETGK +WFNLRHQ AMT Sbjct: 132 GQHLNLPVAALLGEGQQRKSVETLGYLFFVGDISKSRLDYVTGETGKAEWFNLRHQEAMT 191 Query: 191 PEAIVRLAEATQAHYGFADFKLKGGVLRGEEEIEAVTAIAKRFPKARVTLDPNGAWSLDE 250 PE +VRLAEAT HYGFADFKLKGGVLRGE+EIEAVTAIAKRFP ARVTLDPNGAWSLDE Sbjct: 192 PETVVRLAEATHDHYGFADFKLKGGVLRGEQEIEAVTAIAKRFPNARVTLDPNGAWSLDE 251 Query: 251 AIRLCSNMHGVLAYAEDPCGAEAGFSGREIMAEFRRATGLPTATNMIATDWRQLSHALRL 310 A+ LC +MHG+LAYAEDPCGAEAGFSGREIMAEFRRATGLPTATNMIATDWRQLSHALRL Sbjct: 252 AVSLCKDMHGILAYAEDPCGAEAGFSGREIMAEFRRATGLPTATNMIATDWRQLSHALRL 311 Query: 311 GAVDIPLADPHFWTMQGSVRVAQTCRDNGLTWGSHSNNHFDISLAMFTHVGAAAPGKVTA 370 GAVDIPLADPHFWTMQGSVRVAQTCRDNGLTWGSHSNNHFDISLAMFTHVGAAAPGKVTA Sbjct: 312 GAVDIPLADPHFWTMQGSVRVAQTCRDNGLTWGSHSNNHFDISLAMFTHVGAAAPGKVTA 371 Query: 371 IDTHWIWQDGQALTRQPLQIKGGKIAIPDRPGLGIELDRGAIEAANALYKQHGLGARDDA 430 IDTHWIWQDGQALTR+PL+IKGGKIA+PDRPGLGIE+DR AI+AA+ LYKQHGLGARDDA Sbjct: 372 IDTHWIWQDGQALTREPLRIKGGKIAVPDRPGLGIEIDRAAIDAAHDLYKQHGLGARDDA 431 Query: 431 LAMQFLIQGWTFDDKRPCLVR 451 +AMQ LI GWTFDDKRPCLVR Sbjct: 432 IAMQDLIPGWTFDDKRPCLVR 452 >ref|YP_001242303.1| (D)-glucarate dehydratase 1 [Bradyrhizobium sp. BTAi1] gb|ABQ38397.1| D-glucarate dehydratase [Bradyrhizobium sp. BTAi1] Length = 451 Score = 806 bits (2082), Expect = 0.0, Method: Compositional matrix adjust. Identities = 387/451 (85%), Positives = 413/451 (91%) Query: 1 MIQDTIRTGFAGAPVVTAMEVIPVAGRDGMLLNLSGAHAPFFTRNLVILTDNSGHTGVGE 60 M + TG +GAPVV MEVIPVAGRD MLLNLSGAH PFFTRNLVILTDN+GHTGVGE Sbjct: 1 MSHEATSTGISGAPVVIGMEVIPVAGRDSMLLNLSGAHGPFFTRNLVILTDNAGHTGVGE 60 Query: 61 VPGGEKIKKTLEDARDLVVGQTIGAYNNILASMRQAFADRDADGRGKQTFDLRVTIHAVT 120 VPGGEKI++TLEDA+ L+VGQ+IGA N ILASMR AFADRDA GRGKQTFDLRV IHAVT Sbjct: 61 VPGGEKIRQTLEDAKSLIVGQSIGACNTILASMRTAFADRDAGGRGKQTFDLRVMIHAVT 120 Query: 121 AVESALLDLLGQHLNLPVAALLGEGQQRTSVETLGYLFFVGDRRKTSLPYVEGETGKPDW 180 AVESALLDLLGQHL LPVAALLGEGQQRTSVE LGYLFF+GDR+KT LPY GE + W Sbjct: 121 AVESALLDLLGQHLGLPVAALLGEGQQRTSVEALGYLFFIGDRKKTDLPYATGEGERDGW 180 Query: 181 FNLRHQVAMTPEAIVRLAEATQAHYGFADFKLKGGVLRGEEEIEAVTAIAKRFPKARVTL 240 F LRH AMTPEA+VRLAEAT YGFADFKLKGGVL+GE EIEAVTAIAKRFP+ARVTL Sbjct: 181 FTLRHHEAMTPEAVVRLAEATHDAYGFADFKLKGGVLKGEAEIEAVTAIAKRFPQARVTL 240 Query: 241 DPNGAWSLDEAIRLCSNMHGVLAYAEDPCGAEAGFSGREIMAEFRRATGLPTATNMIATD 300 DPNGAWSLDEAIRLC +MHG+LAYAEDPCGAEAGFSGREIMAEFRRATGLPTATNMIATD Sbjct: 241 DPNGAWSLDEAIRLCKDMHGILAYAEDPCGAEAGFSGREIMAEFRRATGLPTATNMIATD 300 Query: 301 WRQLSHALRLGAVDIPLADPHFWTMQGSVRVAQTCRDNGLTWGSHSNNHFDISLAMFTHV 360 WRQLSHALRLGAVDIPLADPHFWT+QGSVRVAQTCRDNGLTWGSHSNNHFDISLAMFTHV Sbjct: 301 WRQLSHALRLGAVDIPLADPHFWTLQGSVRVAQTCRDNGLTWGSHSNNHFDISLAMFTHV 360 Query: 361 GAAAPGKVTAIDTHWIWQDGQALTRQPLQIKGGKIAIPDRPGLGIELDRGAIEAANALYK 420 AAAPGKVTAIDTHWIWQDGQALT+ PLQIKGG+IA+P++PGLGIE+DRGAIE A+ALYK Sbjct: 361 AAAAPGKVTAIDTHWIWQDGQALTKAPLQIKGGRIAVPEQPGLGIEVDRGAIEKAHALYK 420 Query: 421 QHGLGARDDALAMQFLIQGWTFDDKRPCLVR 451 HGLGARDDA+AMQ+LI GWTFDDKRPCLVR Sbjct: 421 SHGLGARDDAVAMQYLIPGWTFDDKRPCLVR 451 >ref|YP_001203683.1| (D)-glucarate dehydratase 1 [Bradyrhizobium sp. ORS 278] emb|CAL75446.1| (D)-glucarate dehydratase 1 [Bradyrhizobium sp. ORS 278] Length = 451 Score = 803 bits (2074), Expect = 0.0, Method: Compositional matrix adjust. Identities = 383/451 (84%), Positives = 411/451 (91%) Query: 1 MIQDTIRTGFAGAPVVTAMEVIPVAGRDGMLLNLSGAHAPFFTRNLVILTDNSGHTGVGE 60 M + TG +GAPVVT MEV+P AGRD MLLNLSGAH PFFTRNLVILTDNSGHTGVGE Sbjct: 1 MSHEATSTGISGAPVVTGMEVVPAAGRDSMLLNLSGAHGPFFTRNLVILTDNSGHTGVGE 60 Query: 61 VPGGEKIKKTLEDARDLVVGQTIGAYNNILASMRQAFADRDADGRGKQTFDLRVTIHAVT 120 VPGGEKI++TLEDA+ L+VGQ+IGA N ILA MR FADRDA GRGKQTFDLRV IHAVT Sbjct: 61 VPGGEKIRQTLEDAKGLIVGQSIGACNTILAKMRSTFADRDAGGRGKQTFDLRVMIHAVT 120 Query: 121 AVESALLDLLGQHLNLPVAALLGEGQQRTSVETLGYLFFVGDRRKTSLPYVEGETGKPDW 180 AVESALLDLLGQHL LPVAALLGEGQQR SVE LGYLFFVGDR+KT LPY G+ + W Sbjct: 121 AVESALLDLLGQHLGLPVAALLGEGQQRKSVEALGYLFFVGDRKKTDLPYATGDGEREGW 180 Query: 181 FNLRHQVAMTPEAIVRLAEATQAHYGFADFKLKGGVLRGEEEIEAVTAIAKRFPKARVTL 240 + LRHQ AMTPEA+VRLAEAT YGFADFKLKGGVL+GE EIEAVTAIAKRFP+ARVTL Sbjct: 181 YTLRHQEAMTPEAVVRLAEATHDAYGFADFKLKGGVLKGEAEIEAVTAIAKRFPQARVTL 240 Query: 241 DPNGAWSLDEAIRLCSNMHGVLAYAEDPCGAEAGFSGREIMAEFRRATGLPTATNMIATD 300 DPNGAWSLDEAIRLC +MHG+LAYAEDPCGAEAGFSGREIMAEFRRATGLPTATNMIATD Sbjct: 241 DPNGAWSLDEAIRLCKDMHGILAYAEDPCGAEAGFSGREIMAEFRRATGLPTATNMIATD 300 Query: 301 WRQLSHALRLGAVDIPLADPHFWTMQGSVRVAQTCRDNGLTWGSHSNNHFDISLAMFTHV 360 WRQLSHALRLGAVDIPLADPHFWTMQGSVRVAQTC+DNGLTWGSHSNNHFDISLAMFTHV Sbjct: 301 WRQLSHALRLGAVDIPLADPHFWTMQGSVRVAQTCKDNGLTWGSHSNNHFDISLAMFTHV 360 Query: 361 GAAAPGKVTAIDTHWIWQDGQALTRQPLQIKGGKIAIPDRPGLGIELDRGAIEAANALYK 420 AAAPGKVTAIDTHWIWQDGQALT+QPLQIKGG+IA+P++PGLG+E+DRGAIE A+ LYK Sbjct: 361 AAAAPGKVTAIDTHWIWQDGQALTKQPLQIKGGRIAVPEQPGLGVEIDRGAIEKAHELYK 420 Query: 421 QHGLGARDDALAMQFLIQGWTFDDKRPCLVR 451 HGLGARDDA+AMQ+LI GWTFDDKRPCLVR Sbjct: 421 SHGLGARDDAVAMQYLIPGWTFDDKRPCLVR 451 >ref|YP_002943647.1| glucarate dehydratase [Variovorax paradoxus S110] gb|ACS18381.1| glucarate dehydratase [Variovorax paradoxus S110] Length = 451 Score = 707 bits (1824), Expect = 0.0, Method: Compositional matrix adjust. Identities = 332/442 (75%), Positives = 384/442 (86%) Query: 10 FAGAPVVTAMEVIPVAGRDGMLLNLSGAHAPFFTRNLVILTDNSGHTGVGEVPGGEKIKK 69 +GAPVVTAM V+PVAG D ML+NLSGAH PFFTRNL+ILTD++GHTGVGEVPGGEKI++ Sbjct: 10 ISGAPVVTAMRVVPVAGHDSMLMNLSGAHGPFFTRNLLILTDSAGHTGVGEVPGGEKIRQ 69 Query: 70 TLEDARDLVVGQTIGAYNNILASMRQAFADRDADGRGKQTFDLRVTIHAVTAVESALLDL 129 TLEDAR L+VGQ +G ++ +L SMR AFA+RD+ GRG+QTFDLRVTIHAVTAVE+ALLDL Sbjct: 70 TLEDARGLIVGQPLGKHHAVLNSMRSAFANRDSGGRGQQTFDLRVTIHAVTAVEAALLDL 129 Query: 130 LGQHLNLPVAALLGEGQQRTSVETLGYLFFVGDRRKTSLPYVEGETGKPDWFNLRHQVAM 189 LGQHL +PVAALLGEGQQR +V+ LGYLF+VGDR +T LPY G DWF LRH+ AM Sbjct: 130 LGQHLEVPVAALLGEGQQRDAVQMLGYLFYVGDRTRTDLPYESDPGGADDWFRLRHEEAM 189 Query: 190 TPEAIVRLAEATQAHYGFADFKLKGGVLRGEEEIEAVTAIAKRFPKARVTLDPNGAWSLD 249 TPEAIVRLAEAT A YGF DFKLKGGVLRGEEE+EA+ A+ +RFPKARVTLDPNG W L Sbjct: 190 TPEAIVRLAEATHARYGFTDFKLKGGVLRGEEEVEAIRALHERFPKARVTLDPNGGWLLA 249 Query: 250 EAIRLCSNMHGVLAYAEDPCGAEAGFSGREIMAEFRRATGLPTATNMIATDWRQLSHALR 309 +AIRLC ++HGV+AYAEDPCGAE FSGRE+MAEFRRATGLPTATNM+ATDWR++ H+L Sbjct: 250 DAIRLCRDLHGVMAYAEDPCGAEGVFSGREVMAEFRRATGLPTATNMVATDWREMVHSLS 309 Query: 310 LGAVDIPLADPHFWTMQGSVRVAQTCRDNGLTWGSHSNNHFDISLAMFTHVGAAAPGKVT 369 L +VDIPLADPHFWTMQGSVRVAQ C+ GLTWGSHSNNHFD+SLAMFTHV AAAPGKVT Sbjct: 310 LQSVDIPLADPHFWTMQGSVRVAQLCQAWGLTWGSHSNNHFDVSLAMFTHVAAAAPGKVT 369 Query: 370 AIDTHWIWQDGQALTRQPLQIKGGKIAIPDRPGLGIELDRGAIEAANALYKQHGLGARDD 429 AIDTHWIWQDGQ LT+ PLQI+GG + +P R GLG+ELD +E A+ LY +HGLGAR+D Sbjct: 370 AIDTHWIWQDGQRLTKAPLQIEGGYVKVPTRGGLGVELDMDEVEKAHQLYLKHGLGARND 429 Query: 430 ALAMQFLIQGWTFDDKRPCLVR 451 A AMQ+LI GWTFD+K+PC+VR Sbjct: 430 AQAMQYLIPGWTFDNKKPCMVR 451 >ref|YP_002502139.1| glucarate dehydratase [Methylobacterium nodulans ORS 2060] gb|ACL61836.1| glucarate dehydratase [Methylobacterium nodulans ORS 2060] Length = 450 Score = 701 bits (1810), Expect = 0.0, Method: Compositional matrix adjust. Identities = 339/440 (77%), Positives = 387/440 (87%), Gaps = 1/440 (0%) Query: 12 GAPVVTAMEVIPVAGRDGMLLNLSGAHAPFFTRNLVILTDNSGHTGVGEVPGGEKIKKTL 71 G P +TAM V+PVAGRD MLLNLSGAH PFFTRNLV+LTD++G TG+GEVPGGE+I++TL Sbjct: 12 GTPTITAMRVVPVAGRDSMLLNLSGAHGPFFTRNLVVLTDSTGATGLGEVPGGERIRRTL 71 Query: 72 EDARDLVVGQTIGAYNNILASMRQAFADRDADGRGKQTFDLRVTIHAVTAVESALLDLLG 131 E+ARDLV+GQ +GA+N +L +MR FADRDA GRG QTFDLRVTIHAVTAVESA LDLLG Sbjct: 72 EEARDLVLGQPLGAWNGVLNAMRTRFADRDAGGRGLQTFDLRVTIHAVTAVESAFLDLLG 131 Query: 132 QHLNLPVAALLGEGQQRTSVETLGYLFFVGDRRKTSLPYVEGETGKPDWFNLRHQVAMTP 191 + L +PVAALLGEGQQR +VE LGYLF+VGDRR+T+LPY E E + W LR + A+TP Sbjct: 132 RFLGVPVAALLGEGQQRDAVEMLGYLFYVGDRRRTNLPYREPE-ARDGWLRLRDEEALTP 190 Query: 192 EAIVRLAEATQAHYGFADFKLKGGVLRGEEEIEAVTAIAKRFPKARVTLDPNGAWSLDEA 251 E +VRLAEA YGF DFKLKGGVL GE+EIEAVTAIAKRFP+ARVTLDPNGAWSL EA Sbjct: 191 ETVVRLAEAAHERYGFNDFKLKGGVLPGEQEIEAVTAIAKRFPQARVTLDPNGAWSLAEA 250 Query: 252 IRLCSNMHGVLAYAEDPCGAEAGFSGREIMAEFRRATGLPTATNMIATDWRQLSHALRLG 311 IRLC VLAYAEDPCGAEAGFSGREIMAEFRRATGLPTATNMIATDWRQ+ HAL+LG Sbjct: 251 IRLCKGRGDVLAYAEDPCGAEAGFSGREIMAEFRRATGLPTATNMIATDWRQMVHALQLG 310 Query: 312 AVDIPLADPHFWTMQGSVRVAQTCRDNGLTWGSHSNNHFDISLAMFTHVGAAAPGKVTAI 371 AVDIPLADPHFWT+ G+VRVAQTCRD+GLTWGSHSNNHFD+SLAMFTH AAAPG VTAI Sbjct: 311 AVDIPLADPHFWTLSGAVRVAQTCRDHGLTWGSHSNNHFDVSLAMFTHAAAAAPGTVTAI 370 Query: 372 DTHWIWQDGQALTRQPLQIKGGKIAIPDRPGLGIELDRGAIEAANALYKQHGLGARDDAL 431 DTHWIWQDGQ LT++PL+I+GG++ +P++PGLGI++D A+EAA+ALY++HGLGARDDA Sbjct: 371 DTHWIWQDGQRLTKEPLRIEGGRVKVPEQPGLGIDIDWAAVEAAHALYERHGLGARDDAA 430 Query: 432 AMQFLIQGWTFDDKRPCLVR 451 AMQFLI GWTFD KRPCLVR Sbjct: 431 AMQFLIPGWTFDPKRPCLVR 450