BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0073.p (445 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_770239.1| hypothetical protein blr3599 [Bradyrhizobium ja... 875 0.0 ref|YP_002362646.1| type VI secretion protein [Methylocella silv... 705 0.0 ref|YP_002498290.1| type VI secretion protein [Methylobacterium ... 668 0.0 ref|ZP_01546335.1| hypothetical protein SIAM614_12788 [Stappia a... 558 e-157 ref|ZP_05741288.1| type VI secretion protein, family [Silicibact... 456 e-126 >ref|NP_770239.1| hypothetical protein blr3599 [Bradyrhizobium japonicum USDA 110] dbj|BAC48864.1| blr3599 [Bradyrhizobium japonicum USDA 110] Length = 449 Score = 875 bits (2261), Expect = 0.0, Method: Compositional matrix adjust. Identities = 422/445 (94%), Positives = 439/445 (98%) Query: 1 MSWFSKVFWSEGLFLRPHHLQQNDRYLEHLLEKRVRYTTPYPWGFAQLEIDNDLTQQSKF 60 MSW+SKVFWSEGLFLRPHHLQQNDRY+EHLLEKRVRYTTPYPWGFAQLEID DLT+QSKF Sbjct: 5 MSWYSKVFWSEGLFLRPHHLQQNDRYIEHLLEKRVRYTTPYPWGFAQLEIDKDLTEQSKF 64 Query: 61 AVRSATGVMPDGTPFDIPADSPIPEAIEVPDSAAGQIVWLMMPVAAPNTREVDEDRTDSA 120 AVR+A+GVMPDGTPFDIPADSPIPEAI+VP++AAGQIVWLMMPVAAPNTREVDEDRTDSA Sbjct: 65 AVRNASGVMPDGTPFDIPADSPIPEAIDVPETAAGQIVWLMMPVAAPNTREVDEDRTDSA 124 Query: 121 SRYVRTAETFIDSTSALRIEEEIDIVYPRLSFELRKTSKPGYMGLGIARILEVRDKNILF 180 SRYVRT+ETFIDSTSALRIEEEIDI YPRLSFELRKTSKPGYMGLGIARILEVRDKNILF Sbjct: 125 SRYVRTSETFIDSTSALRIEEEIDIAYPRLSFELRKTSKPGYMGLGIARILEVRDKNILF 184 Query: 181 DDKFVPPMLVCAAHPVVDGWLNRIIGWVETKLDELARYAVDPSSGGGLQSVDYLVLQVLN 240 DDKFVPPMLVCAAHP++DGWLNRIIGWVETKLDELARYAVDPSSGGGLQSVDYLVLQVLN Sbjct: 185 DDKFVPPMLVCAAHPIIDGWLNRIIGWVETKLDELARYAVDPSSGGGLQSVDYLVLQVLN 244 Query: 241 RTIPVLTHFKRSGSVHPERLYEELLRFAGELATFATTERRARDYPAYDHDNLEYVFTPLV 300 RTIPVLTHF+RSGSVHPERLYEELLRFAGELATF+T ERRAR+YPAYDHD+LE VFTPLV Sbjct: 245 RTIPVLTHFQRSGSVHPERLYEELLRFAGELATFSTAERRARNYPAYDHDDLENVFTPLV 304 Query: 301 RDIQDFLSARLGRRAIRLEIIERAQNAFVSPIRDRALFRNATLVLEVAARRPLVEIQNEF 360 DIQDFLSARLGRRAIRLEIIERAQNAFVSPIRDRALFRNATLVLEVAARRPLVEIQN+F Sbjct: 305 SDIQDFLSARLGRRAIRLEIIERAQNAFVSPIRDRALFRNATLVLEVAARRPLVEIQNDF 364 Query: 361 PHLFKVGPNTKMTEIVHANLPGLTLVHLPTPPPHIRAITDHVYFYLDRKSPLWPEFSTAA 420 PHLFKVGPNTKM EIVHANLPGLTLVHLPTPPPHIRAITDHVYFYLDRKSPLWPEFSTAA Sbjct: 365 PHLFKVGPNTKMNEIVHANLPGLTLVHLPTPPPHIRAITDHVYFYLDRKSPLWPEFSTAA 424 Query: 421 SIGMHFSGDWPELELYLWAILEERP 445 SIGMHFSGDWPELELYLWAILE+RP Sbjct: 425 SIGMHFSGDWPELELYLWAILEDRP 449 >ref|YP_002362646.1| type VI secretion protein [Methylocella silvestris BL2] gb|ACK51284.1| type VI secretion protein, VC_A0114 family [Methylocella silvestris BL2] Length = 446 Score = 705 bits (1819), Expect = 0.0, Method: Compositional matrix adjust. Identities = 339/444 (76%), Positives = 385/444 (86%) Query: 1 MSWFSKVFWSEGLFLRPHHLQQNDRYLEHLLEKRVRYTTPYPWGFAQLEIDNDLTQQSKF 60 MSW+SKV WSEGLFLRPHHLQQ DRYLEHL+E R R TPYPWGF+ LEID DL QQSKF Sbjct: 2 MSWYSKVLWSEGLFLRPHHLQQADRYLEHLIEARTRSATPYPWGFSHLEIDRDLAQQSKF 61 Query: 61 AVRSATGVMPDGTPFDIPADSPIPEAIEVPDSAAGQIVWLMMPVAAPNTREVDEDRTDSA 120 AVR A GVMPDGTPFDIP DSP P +EVPD AA Q++WL MP A+ NTREVD + SA Sbjct: 62 AVRRAAGVMPDGTPFDIPNDSPAPPPLEVPDGAAQQLLWLSMPTASSNTREVDSADSSSA 121 Query: 121 SRYVRTAETFIDSTSALRIEEEIDIVYPRLSFELRKTSKPGYMGLGIARILEVRDKNILF 180 SR+V AETFIDSTS+LRIEEEID+ YPRL+F+LRKT+KPG+ GL IARI+EVRDK+I+F Sbjct: 122 SRFVIGAETFIDSTSSLRIEEEIDVAYPRLAFDLRKTAKPGFQGLPIARIVEVRDKSIVF 181 Query: 181 DDKFVPPMLVCAAHPVVDGWLNRIIGWVETKLDELARYAVDPSSGGGLQSVDYLVLQVLN 240 D+KF P +LVC+AHP V+GWL+R+IGW++ KL ELARYA DP++GGGLQSVDYLVLQVLN Sbjct: 182 DEKFAPTVLVCSAHPTVEGWLDRVIGWIDAKLGELARYAADPTAGGGLQSVDYLVLQVLN 241 Query: 241 RTIPVLTHFKRSGSVHPERLYEELLRFAGELATFATTERRARDYPAYDHDNLEYVFTPLV 300 R I V+ H + S VHPERLY EL+R AGELATFATTERRAR YP YDHDNLE F P++ Sbjct: 242 RHITVMQHLRGSRYVHPERLYVELIRLAGELATFATTERRARLYPLYDHDNLEASFAPVL 301 Query: 301 RDIQDFLSARLGRRAIRLEIIERAQNAFVSPIRDRALFRNATLVLEVAARRPLVEIQNEF 360 RD+QDFLSARLGRRAIRL+II RA+NAF+SPIRDR+LFRNA+LVLEVAARRPL EIQ+ F Sbjct: 302 RDLQDFLSARLGRRAIRLDIIARAENAFISPIRDRSLFRNASLVLEVAARRPLTEIQSSF 361 Query: 361 PHLFKVGPNTKMTEIVHANLPGLTLVHLPTPPPHIRAITDHVYFYLDRKSPLWPEFSTAA 420 PHLFKVGP +KM EIVHA+LPG+ LVHLPTPPP IRAITDHVYFYLDR SPLWPEFSTA+ Sbjct: 362 PHLFKVGPGSKMNEIVHAHLPGVPLVHLPTPPPQIRAITDHVYFYLDRTSPLWPEFSTAS 421 Query: 421 SIGMHFSGDWPELELYLWAILEER 444 SIGMHFSGDWPEL L LWA+LE+R Sbjct: 422 SIGMHFSGDWPELSLELWAVLEDR 445 >ref|YP_002498290.1| type VI secretion protein [Methylobacterium nodulans ORS 2060] gb|ACL57987.1| type VI secretion protein, VC_A0114 family [Methylobacterium nodulans ORS 2060] Length = 445 Score = 668 bits (1724), Expect = 0.0, Method: Compositional matrix adjust. Identities = 308/444 (69%), Positives = 372/444 (83%) Query: 1 MSWFSKVFWSEGLFLRPHHLQQNDRYLEHLLEKRVRYTTPYPWGFAQLEIDNDLTQQSKF 60 MSW SKV WSEGLFLRP HLQQ DRY+EHL+E RVR TPYPWGF+ LEID+DL QQ +F Sbjct: 1 MSWTSKVAWSEGLFLRPQHLQQADRYVEHLVEARVRPVTPYPWGFSVLEIDHDLAQQGRF 60 Query: 61 AVRSATGVMPDGTPFDIPADSPIPEAIEVPDSAAGQIVWLMMPVAAPNTREVDEDRTDSA 120 +R A G++PDGTPFD+P +P+P +EVP+ A ++WL +PVAA NTRE+D + D+A Sbjct: 61 GLRRAAGILPDGTPFDVPGHAPLPAPVEVPEGAGQHLLWLTLPVAAANTRELDAEEADTA 120 Query: 121 SRYVRTAETFIDSTSALRIEEEIDIVYPRLSFELRKTSKPGYMGLGIARILEVRDKNILF 180 SRYV E IDST+ LR+EEEI+I +PRL+ +LR+T+KPGY+ L +AR+LEVRD ++ Sbjct: 121 SRYVHATELLIDSTAPLRVEEEIEIAHPRLALDLRRTAKPGYVVLPVARVLEVRDHTVVL 180 Query: 181 DDKFVPPMLVCAAHPVVDGWLNRIIGWVETKLDELARYAVDPSSGGGLQSVDYLVLQVLN 240 D++FVPP+L CAAHPV DGW++R++GW+ET+LDELARYA DP++GGGLQS DY VLQ+LN Sbjct: 181 DERFVPPVLSCAAHPVADGWIDRVVGWIETRLDELARYAADPTAGGGLQSADYFVLQLLN 240 Query: 241 RTIPVLTHFKRSGSVHPERLYEELLRFAGELATFATTERRARDYPAYDHDNLEYVFTPLV 300 R +PVL HF+ S VHPERLYEE LR AGELATFAT ERRAR YP Y+ ++LE VF PL+ Sbjct: 241 RHLPVLRHFRASRCVHPERLYEEFLRIAGELATFATIERRARAYPVYNQNDLEGVFGPLL 300 Query: 301 RDIQDFLSARLGRRAIRLEIIERAQNAFVSPIRDRALFRNATLVLEVAARRPLVEIQNEF 360 RDIQDFLSA++GRRAIRLEI+ERA NAFVS IRDR LFRNA LVLEV+ARRPL EIQ +F Sbjct: 301 RDIQDFLSAQIGRRAIRLEIVERAPNAFVSTIRDRNLFRNANLVLEVSARRPLTEIQAQF 360 Query: 361 PHLFKVGPNTKMTEIVHANLPGLTLVHLPTPPPHIRAITDHVYFYLDRKSPLWPEFSTAA 420 PHLFK+GPNTKM EIVHA+LPG+ LVHLPTPPP IRA+ DHVYF LDR+SPLWPEFS A+ Sbjct: 361 PHLFKIGPNTKMNEIVHAHLPGVGLVHLPTPPPQIRAMADHVYFLLDRQSPLWPEFSVAS 420 Query: 421 SIGMHFSGDWPELELYLWAILEER 444 S+GMHFSGDWP+L L LWA+LE R Sbjct: 421 SLGMHFSGDWPDLALELWAVLEGR 444 >ref|ZP_01546335.1| hypothetical protein SIAM614_12788 [Stappia aggregata IAM 12614] gb|EAV44891.1| hypothetical protein SIAM614_12788 [Stappia aggregata IAM 12614] Length = 444 Score = 558 bits (1438), Expect = e-157, Method: Compositional matrix adjust. Identities = 263/444 (59%), Positives = 332/444 (74%) Query: 1 MSWFSKVFWSEGLFLRPHHLQQNDRYLEHLLEKRVRYTTPYPWGFAQLEIDNDLTQQSKF 60 MSW+SKV W+EGLFLRPHHLQQ+DRY EHL+++RVR+ +PYPWGF++LEID D+ QQ +F Sbjct: 1 MSWYSKVAWTEGLFLRPHHLQQSDRYAEHLVDERVRFASPYPWGFSELEIDQDVGQQCRF 60 Query: 61 AVRSATGVMPDGTPFDIPADSPIPEAIEVPDSAAGQIVWLMMPVAAPNTREVDEDRTDSA 120 A+R G+ DGT F+ PAD P AI+VP+ AAGQIVWL +P N EV R D+A Sbjct: 61 ALRYGRGIFSDGTVFNFPADGTPPPAIDVPEDAAGQIVWLGIPETTVNAVEVANGRDDAA 120 Query: 121 SRYVRTAETFIDSTSALRIEEEIDIVYPRLSFELRKTSKPGYMGLGIARILEVRDKNILF 180 +RY++ ET IDSTS +R EEEID+ +PRL + +R T PG + L +ARI E++D+ ++ Sbjct: 121 TRYIQGRETIIDSTSTIRQEEEIDVAHPRLQYSIRSTPHPGQVCLPVARITEIKDRIVIT 180 Query: 181 DDKFVPPMLVCAAHPVVDGWLNRIIGWVETKLDELARYAVDPSSGGGLQSVDYLVLQVLN 240 D F PP+L C HPVV GW++ +IGWVE KL ELARYA D S+GGGLQ+ DYL LQ+LN Sbjct: 181 DGTFAPPVLACHVHPVVTGWIDSVIGWVERKLSELARYAADTSAGGGLQNFDYLTLQLLN 240 Query: 241 RTIPVLTHFKRSGSVHPERLYEELLRFAGELATFATTERRARDYPAYDHDNLEYVFTPLV 300 RT P+L H ++S VHPERLY L+ AGELATFAT ERRAR+YP YDHD L VF P++ Sbjct: 241 RTYPLLMHLRKSAYVHPERLYSVFLQLAGELATFATEERRARNYPVYDHDKLHDVFKPVL 300 Query: 301 RDIQDFLSARLGRRAIRLEIIERAQNAFVSPIRDRALFRNATLVLEVAARRPLVEIQNEF 360 DIQ FLSA RRAIRL + + A NAF S I DR LF A V+EVAARRPL EIQNEF Sbjct: 301 ADIQAFLSAGYDRRAIRLPLEQLAPNAFKSTIVDRNLFSQAGFVIEVAARRPLTEIQNEF 360 Query: 361 PHLFKVGPNTKMTEIVHANLPGLTLVHLPTPPPHIRAITDHVYFYLDRKSPLWPEFSTAA 420 P LFKVGP +M +IV++NLPG+ L+H+P PP IRA+TD VYF LD+ +PLW EFS + Sbjct: 361 PRLFKVGPFGQMRQIVYSNLPGIPLIHMPVPPAQIRALTDRVYFRLDKTTPLWREFSQDS 420 Query: 421 SIGMHFSGDWPELELYLWAILEER 444 +IG+ FSG+WP+L+L +A+ E R Sbjct: 421 AIGLQFSGNWPDLQLEFYAVREGR 444 >ref|ZP_05741288.1| type VI secretion protein, family [Silicibacter sp. TrichCH4B] gb|EEW58089.1| type VI secretion protein, family [Silicibacter sp. TrichCH4B] Length = 444 Score = 456 bits (1174), Expect = e-126, Method: Compositional matrix adjust. Identities = 226/443 (51%), Positives = 298/443 (67%), Gaps = 2/443 (0%) Query: 1 MSWFSKVFWSEGLFLRPHHLQQNDRYLEHLLEKRVRYTTPYPWGFAQLEIDNDLTQQSKF 60 MS FSKV W EGLFL+PHHLQQ DRYLEH L +R TPYPWG ++L D DL QQ K Sbjct: 1 MSNFSKVAWKEGLFLQPHHLQQADRYLEHQLRERTATITPYPWGVSELRFDRDLAQQGKI 60 Query: 61 AVRSATGVMPDGTPFDIPADSPIPEAIEV-PDSAAGQIVWLMMPVAAPNTREVDEDRTDS 119 +R+ G+MPDG FD P P+P + V D A G VWL +P A+ + ++V D+ Sbjct: 61 GLRAVAGIMPDGIAFDAPGSVPLPVPVSVPEDEAQGLDVWLTLPDASVSGQDVSPAAADA 120 Query: 120 ASRYVRTAETFIDSTSALRIEEEIDIVYPRLSFELRKTSKPGYMGLGIARILEVRDKNIL 179 A+RYV AET +++ S R E ++I PRL LRKT +PGY + + RI+E+RD + Sbjct: 121 ATRYVLDAETVVNNASNSRSEHLVEIAQPRLELVLRKTPRPGYQNIRLGRIIEMRDGIVT 180 Query: 180 FDDKFVPPMLVCAAHPVVDGWLNRIIGWVETKLDELARYAVDPSSGGGLQSVDYLVLQVL 239 DD P LV HP +G+L+R+IGW+E KLD LAR+A DP+SGGG+Q+ DYL+L VL Sbjct: 181 LDDTVPPVGLVTRVHPAYEGYLSRVIGWIEAKLDTLARFAADPTSGGGMQATDYLMLMVL 240 Query: 240 NRTIPVLTHFKRSGSVHPERLYEELLRFAGELATFATTERRARDYPAYDHDNLEYVFTPL 299 NR +PVL H + ++HPERLYE+LL AGEL+TF R+A DY YDH FT L Sbjct: 241 NRELPVLRHMQGLANLHPERLYEKLLALAGELSTFDHGARKAPDYARYDHQASRSGFTAL 300 Query: 300 VRDIQDFLSARLGRRAIRLEIIERAQNAFVSPIRDRALFRNATLVLEVAARRPLVEIQNE 359 V+DIQ L+ +G RA+RL + E N++ + + DR+LF AT V+EV++ PL ++Q E Sbjct: 301 VQDIQQLLARDVG-RAVRLPLNEVRANSYAALVNDRSLFAKATFVIEVSSGLPLTQVQQE 359 Query: 360 FPHLFKVGPNTKMTEIVHANLPGLTLVHLPTPPPHIRAITDHVYFYLDRKSPLWPEFSTA 419 FP L KVGP+T M +IV NLPG+ LVHLP PP IR ++ VYF LD+ S LW EFSTA Sbjct: 360 FPRLCKVGPSTAMKQIVSNNLPGIGLVHLPNPPRQIRVVSTSVYFLLDKSSDLWREFSTA 419 Query: 420 ASIGMHFSGDWPELELYLWAILE 442 +IGMHF+G+WP+++L WA+ E Sbjct: 420 PAIGMHFAGNWPDMKLECWAVPE 442