BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0050.p (538 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_770215.1| phosphoenolpyruvate-protein phosphotransferase ... 909 0.0 ref|NP_105981.1| phosphoenolpyruvate-protein phosphotransferase ... 456 e-126 ref|YP_004608807.1| phosphoenolpyruvate-protein phosphotransfera... 455 e-125 ref|YP_004139438.1| phosphoenolpyruvate-protein phosphotransfera... 444 e-122 ref|YP_001206562.1| phosphotransferase system enzyme I [Bradyrhi... 431 e-118 >ref|NP_770215.1| phosphoenolpyruvate-protein phosphotransferase [Bradyrhizobium japonicum USDA 110] dbj|BAC48840.1| ptsI [Bradyrhizobium japonicum USDA 110] Length = 539 Score = 909 bits (2348), Expect = 0.0, Method: Compositional matrix adjust. Identities = 477/538 (88%), Positives = 498/538 (92%), Gaps = 1/538 (0%) Query: 1 MQGGAAGLAYRGRTASIGFAHGPFVRVDAGAAGERVAGTLVEEALALRNAIDVASGQIAD 60 MQG A GLAYRGRTASIGFAHGP VRV A GERVAG L EEALALR AID ASGQIAD Sbjct: 1 MQGSATGLAYRGRTASIGFAHGPLVRVGADTNGERVAGNLAEEALALRAAIDAASGQIAD 60 Query: 61 LAASAGGEAAQILEFQVALLEDEDLIEAVFASIGEGRPADIAWRSTLDAHIADYNSAPDD 120 LA AGGEAAQILEFQVALLEDED I+A+FASIG+G PAD+AWRS LDA IADYNSA D+ Sbjct: 61 LAGIAGGEAAQILEFQVALLEDEDFIQAIFASIGDGDPADVAWRSALDAQIADYNSAADE 120 Query: 121 YLKARSSDLADLRDRVVHILRGDQGQPLKVPSGGVVCADDLPPSRFLEIDWSGGGGLALL 180 YLKARSSDLADLRDRV++ILRGD G L++PSG VVCADDLPPSRFLEIDWSGGGGLALL Sbjct: 121 YLKARSSDLADLRDRVINILRGDGGPALEIPSGAVVCADDLPPSRFLEIDWSGGGGLALL 180 Query: 181 RGSPTSHVAMLARARGIPMVVQLGTIPDIGTTALLDGEGATLELDPNAEQVRTFEKRRDS 240 RGSPTSHVAMLARARGIPMVVQLG IPD+ ALLDGEGATLELDP+AEQVR FEKRR+S Sbjct: 181 RGSPTSHVAMLARARGIPMVVQLGAIPDVRANALLDGEGATLELDPSAEQVRLFEKRRES 240 Query: 241 HLKSRASARAILRRPTASWRGERIKLFINIQRVEDLEHADAQYADGIGLMRTEFLLAGR- 299 H KSRASARAILRRPTASWRGERIKLFINIQRV+DLEH DAQYADGIGLMRTEFLL R Sbjct: 241 HRKSRASARAILRRPTASWRGERIKLFINIQRVDDLEHPDAQYADGIGLMRTEFLLTERG 300 Query: 300 SVPDEETQFQAYDAVLRWAGQRPVTIRTFDAGGDKPVPGFTLDGEANPFLGVRGLRLCLA 359 S+PDEETQFQAYDAVLRWA QRPVTIRTFDAGGDKPV GFTLDGEANPFLGVRGLRLCLA Sbjct: 301 SLPDEETQFQAYDAVLRWADQRPVTIRTFDAGGDKPVAGFTLDGEANPFLGVRGLRLCLA 360 Query: 360 RPEIFAVQLRALARAAIRGNLKVMFPMVTSADELEAGRKLFADIVQRLQADGIAAMLPEL 419 RPEIFA+QLRALARAA+RGNLKVMFPMVTSA ELEAGRKLFAD+VQRLQADGIAAMLPEL Sbjct: 361 RPEIFAIQLRALARAAVRGNLKVMFPMVTSAAELEAGRKLFADVVQRLQADGIAAMLPEL 420 Query: 420 GIMVEVPAAALAVSSFKAAFFSIGSNDLAQYVLACDRSNGALAPLMDPLHPAVLELIART 479 GIMVEVPAAALA++SFK +FFSIGSNDLAQYVLACDRSNGALAPLMDPLHPAVLELIART Sbjct: 421 GIMVEVPAAALAITSFKTSFFSIGSNDLAQYVLACDRSNGALAPLMDPLHPAVLELIART 480 Query: 480 AEHGRRAGISVSLCGDMAGDPRCLPALLNCGLRELSVNASALAQIKHTIDRLSSGGSV 537 AEHGRRA ISVSLCGDMA DPRCLPALLNCGLRELSVNASALAQIK TIDRLSSGG + Sbjct: 481 AEHGRRAAISVSLCGDMASDPRCLPALLNCGLRELSVNASALAQIKQTIDRLSSGGGL 538 >ref|NP_105981.1| phosphoenolpyruvate-protein phosphotransferase [Mesorhizobium loti MAFF303099] dbj|BAB51767.1| phosphoenolpyruvate-protein phosphotransferase [Mesorhizobium loti MAFF303099] Length = 527 Score = 456 bits (1173), Expect = e-126, Method: Compositional matrix adjust. Identities = 258/523 (49%), Positives = 336/523 (64%), Gaps = 6/523 (1%) Query: 12 GRTASIGFAHGPFVRVDAGAAGERVAGTLVEEALALRNAIDVASGQIADLAASAGGEAAQ 71 G AS G+A GP +D A TL EE +L+ AI A +++ +A +A +AA Sbjct: 8 GVPASAGYAEGPLFDLDRPPAVYAGKATLAEEKTSLQAAIGTAVSRLSAMAEAADSDAAG 67 Query: 72 ILEFQVALLEDEDLIEAVFASIGEGRPADIAWRSTLDAHIADYNSAPDDYLKARSSDLAD 131 ILEF +A+LED+ L FASI G+ AD+AWR LDA IA Y ++ DY +AR++DL D Sbjct: 68 ILEFHIAMLEDDALRGPAFASITSGQTADVAWREALDAEIAGYEASDQDYFRARATDLRD 127 Query: 132 LRDRVVHILRGDQGQPLKVPSGGVVCADDLPPSRFLEIDWSGGGGLALLRGSPTSHVAML 191 +RD+V+ L D P+G ++C +D+ P+RFLE DWS GGG+AL GS SHVAML Sbjct: 128 IRDQVLRALTEDVDS--AAPTGAILCGEDIAPTRFLETDWSHGGGIALKAGSTASHVAML 185 Query: 192 ARARGIPMVVQLGTIP-DIGTTALLDGEGATLELDPNAEQVRTFEKRRDSHLKSRASARA 250 AR+RG+PMVV L + ALLD E ++ L P+ ++ F + S + A+ Sbjct: 186 ARSRGVPMVVGLREMSASPAGMALLDAEHGSIVLAPSPAEIGAFRQSSASFAARQGKAQR 245 Query: 251 ILRRPTASWRGERIKLFINIQRVEDLEHADAQYADGIGLMRTEFLLAGRSVPDEETQFQA 310 L RP + G +++ +NI D++ D DG+GLMRTEFL G+++PDEETQ++A Sbjct: 246 FLARPAVTKAGTAVRVQVNIADPSDVDGIDVATCDGVGLMRTEFLF-GKNLPDEETQYRA 304 Query: 311 YDAVLRWAGQRPVTIRTFDAGGDKPVPGFTLDGEANPFLGVRGLRLCLARPEIFAVQLRA 370 Y VL WAG++PVTIRT DAGGDKPVPGFT++ E NPFLG+RG+RL LAR +IF +Q+RA Sbjct: 305 YRKVLEWAGEKPVTIRTVDAGGDKPVPGFTVE-EGNPFLGLRGIRLSLARLDIFRIQIRA 363 Query: 371 LARAAIRGNLKVMFPMVTSADELEAGRKLFADIVQRLQADGIAAMLPELGIMVEVPAAAL 430 L RAA GNL+VMFPM+ ADE E LFA+ L A G+A +P LGIMVEVP+ A+ Sbjct: 364 LLRAAPHGNLRVMFPMIAMADEYERAAALFAEEQAALAAGGVAQKMPPLGIMVEVPSVAI 423 Query: 431 AVSSFK-AAFFSIGSNDLAQYVLACDRSNGALAPLMDPLHPAVLELIARTAEHGRRAGIS 489 A +F AFFSIGSNDL QYV+A R N A+A HPAVL LI A GR GI Sbjct: 424 APEAFAGVAFFSIGSNDLTQYVMAAARDNAAVAHFNSVRHPAVLRLIGSVAAFGRENGIP 483 Query: 490 VSLCGDMAGDPRCLPALLNCGLRELSVNASALAQIKHTIDRLS 532 VSLCGD GDP +P+LL GLR+LSV + LA K I +S Sbjct: 484 VSLCGDAGGDPAAIPSLLEAGLRDLSVAPAQLAMAKAAIADVS 526 >ref|YP_004608807.1| phosphoenolpyruvate-protein phosphotransferase [Mesorhizobium opportunistum WSM2075] gb|AEH84713.1| phosphoenolpyruvate-protein phosphotransferase [Mesorhizobium opportunistum WSM2075] Length = 524 Score = 455 bits (1170), Expect = e-125, Method: Compositional matrix adjust. Identities = 258/523 (49%), Positives = 330/523 (63%), Gaps = 6/523 (1%) Query: 12 GRTASIGFAHGPFVRVDAGAAGERVAGTLVEEALALRNAIDVASGQIADLAASAGGEAAQ 71 G AS G+A GP +D A T EE +LR AI A ++ + +A +AA Sbjct: 5 GVPASAGYAEGPLFELDRPPAVYASKATPEEEKTSLREAIGKAVSRLMAMVETADSDAAG 64 Query: 72 ILEFQVALLEDEDLIEAVFASIGEGRPADIAWRSTLDAHIADYNSAPDDYLKARSSDLAD 131 +LEF +A+LED+ L FASI GR AD AWR LD IA Y ++ DY +AR+ DL D Sbjct: 65 VLEFHIAMLEDDALSGPAFASIDSGRTADAAWRQALDGEIAGYEASDQDYFRARAGDLRD 124 Query: 132 LRDRVVHILRGDQGQPLKVPSGGVVCADDLPPSRFLEIDWSGGGGLALLRGSPTSHVAML 191 +RD+V+H L D G P+G ++ +D+ P+RFLE DWS GGG+AL GS SHVAML Sbjct: 125 IRDQVLHALTDDGGS--AAPAGAILYGEDIAPTRFLETDWSSGGGIALRAGSTASHVAML 182 Query: 192 ARARGIPMVVQLGTIP-DIGTTALLDGEGATLELDPNAEQVRTFEKRRDSHLKSRASARA 250 AR+RG+PMVV L P + ALLD E + L P+ ++ +F + S + A+ Sbjct: 183 ARSRGVPMVVSLREPPAGLAGMALLDAEHGNIVLAPSPAEIGSFRQSSSSFAARQGKAQT 242 Query: 251 ILRRPTASWRGERIKLFINIQRVEDLEHADAQYADGIGLMRTEFLLAGRSVPDEETQFQA 310 L RP + G +++ +NI D++ D DG+GLMRTEFL G+++PDEETQ++ Sbjct: 243 FLARPALTKAGTAVRVQVNIADPSDVDAIDIATCDGVGLMRTEFLF-GKTLPDEETQYRV 301 Query: 311 YDAVLRWAGQRPVTIRTFDAGGDKPVPGFTLDGEANPFLGVRGLRLCLARPEIFAVQLRA 370 Y VL WAG +PVTIRT DAGGDKPVPGFT++ E NPFLG+RG+RL LAR +IF VQ+RA Sbjct: 302 YRKVLEWAGDKPVTIRTVDAGGDKPVPGFTVE-EGNPFLGLRGIRLSLARLDIFRVQIRA 360 Query: 371 LARAAIRGNLKVMFPMVTSADELEAGRKLFADIVQRLQADGIAAMLPELGIMVEVPAAAL 430 L RAA GNL+VMFPM+ +A+E E LF + L A G A +P LGIMVEVP+ A+ Sbjct: 361 LLRAAPHGNLRVMFPMIATAEEYERAAALFTEEQAALTARGTAQRMPPLGIMVEVPSVAI 420 Query: 431 AVSSF-KAAFFSIGSNDLAQYVLACDRSNGALAPLMDPLHPAVLELIARTAEHGRRAGIS 489 A +F AAFFSIGSNDL QYV+A R N A+A L HPAVL LI GR GI Sbjct: 421 APEAFANAAFFSIGSNDLTQYVMAAARDNAAVAHLNSVRHPAVLRLIGSVTAFGRDNGIP 480 Query: 490 VSLCGDMAGDPRCLPALLNCGLRELSVNASALAQIKHTIDRLS 532 VSLCGD GDP +PALL GLR+LSV + LA K I +S Sbjct: 481 VSLCGDAGGDPTLIPALLEAGLRDLSVAPAQLAMAKAAIADIS 523 >ref|YP_004139438.1| phosphoenolpyruvate-protein phosphotransferase [Mesorhizobium ciceri biovar biserrulae WSM1271] gb|ADV09388.1| phosphoenolpyruvate-protein phosphotransferase [Mesorhizobium ciceri biovar biserrulae WSM1271] Length = 528 Score = 444 bits (1143), Expect = e-122, Method: Compositional matrix adjust. Identities = 256/527 (48%), Positives = 340/527 (64%), Gaps = 6/527 (1%) Query: 8 LAYRGRTASIGFAHGPFVRVDAGAAGERVAGTLVEEALALRNAIDVASGQIADLAASAGG 67 L +G +AS G+A GP +D T +E AL AI VA+ ++ L+ A G Sbjct: 5 LRLQGISASAGYAEGPLFDLDQTVVSYVGKETADDEKAALEAAIAVATSRLTALSEMAEG 64 Query: 68 EAAQILEFQVALLEDEDLIEAVFASIGEGRPADIAWRSTLDAHIADYNSAPDDYLKARSS 127 +AA ILEFQ+A+L D+ L FA+I G PAD AWR LD IA Y+++ DY +AR++ Sbjct: 65 DAADILEFQIAMLRDDALSSPAFAAIRTGLPADKAWRQALDTEIAGYDASDQDYFRARAA 124 Query: 128 DLADLRDRVVHILRGDQGQPLKVPSGGVVCADDLPPSRFLEIDWSGGGGLALLRGSPTSH 187 D+ D+RD+V+ L D ++ P+G ++ D+ P+RFLE DWS GGG+AL GS SH Sbjct: 125 DIRDIRDQVLRALSEDGD--VQAPTGAILYGWDIAPTRFLETDWSRGGGIALKAGSTASH 182 Query: 188 VAMLARARGIPMVVQLGT-IPDIGTTALLDGEGATLELDPNAEQVRTFEKRRDSHLKSRA 246 VAMLAR+RG+PMVV LGT + + TALLD E + L P+ ++ F + S Sbjct: 183 VAMLARSRGVPMVVGLGTSLARLTGTALLDAEHGGIVLSPSKTEIAAFRQSSSSFRARSD 242 Query: 247 SARAILRRPTASWRGERIKLFINIQRVEDLEHADAQYADGIGLMRTEFLLAGRSVPDEET 306 A L RP + G +++ +NI D++ D DG+GLMRTEFL G+++PDEET Sbjct: 243 RAGTFLARPAVTKAGTAVRVQVNIADPSDVDLIDITTCDGVGLMRTEFLF-GKTLPDEET 301 Query: 307 QFQAYDAVLRWAGQRPVTIRTFDAGGDKPVPGFTLDGEANPFLGVRGLRLCLARPEIFAV 366 Q++AY VL WAG++PVTIRT DAGGDKPVPGFT++ E+NPFLG+RG+RL LARPE+F V Sbjct: 302 QYRAYRKVLEWAGEKPVTIRTVDAGGDKPVPGFTVE-ESNPFLGLRGIRLSLARPEVFRV 360 Query: 367 QLRALARAAIRGNLKVMFPMVTSADELEAGRKLFADIVQRLQADGIAAMLPELGIMVEVP 426 Q+RAL RAA+ G+LKVMFPM+ +E +FA+ L A G+A +P LGIMVEVP Sbjct: 361 QIRALLRAAVHGSLKVMFPMIAIVEEYRQAAAMFAEEQTALAARGVAHNMPPLGIMVEVP 420 Query: 427 AAALAVSSFK-AAFFSIGSNDLAQYVLACDRSNGALAPLMDPLHPAVLELIARTAEHGRR 485 + A+ +F AAFFSIGSNDL QYV+A R N A+A L HPAVL LIA G+R Sbjct: 421 SVAIVPEAFTDAAFFSIGSNDLTQYVMAAARDNAAVAHLNSVRHPAVLRLIAAVTAFGQR 480 Query: 486 AGISVSLCGDMAGDPRCLPALLNCGLRELSVNASALAQIKHTIDRLS 532 I VSLCGD AGDP +P+L+ GLR+LSV + LA K + +S Sbjct: 481 EEIPVSLCGDAAGDPASIPSLIEAGLRDLSVAPAQLAMAKAAVADVS 527 >ref|YP_001206562.1| phosphotransferase system enzyme I [Bradyrhizobium sp. ORS 278] emb|CAL78343.1| Phosphotransferase system enzyme I (Phosphoenolpyruvate-protein phosphotransferase) (major [Bradyrhizobium sp. ORS 278] Length = 535 Score = 431 bits (1108), Expect = e-118, Method: Compositional matrix adjust. Identities = 264/531 (49%), Positives = 331/531 (62%), Gaps = 7/531 (1%) Query: 11 RGRTASIGFAHGPFVRVDAGAAGERVAGTLVEEALALRNAIDVASGQIADLAASAGGEAA 70 RGR AS G A GP V A R G++ EE AL A+ A +A+L AS G+AA Sbjct: 7 RGRAASEGLAMGPAVIRRALEGASRAKGSIAEEHAALAAAVARARDAVAELIASLQGDAA 66 Query: 71 QILEFQVALLEDEDLIEAVFASIGEGRPADIAWRSTLDAHIADYNSAPDDYLKARSSDLA 130 +++ FQ+ALLED+ L EA FA I G A+ AWR+ + IA Y SA D+Y +AR+SDLA Sbjct: 67 ELIGFQLALLEDDALTEAAFAQIERGTAAEDAWRAAMAQEIAGYESADDEYFRARASDLA 126 Query: 131 DLRDRVVHILRG--DQGQPLKVPSGGVVCADDLPPSRFLEIDWSGGGGLALLRGSPTSHV 188 D+RDRVV L G D GQ +PSG ++ A DL PS FL +DWS GGG+AL GSPTSHV Sbjct: 127 DIRDRVVRHLFGLADDGQ---LPSGAIIVARDLAPSAFLGVDWSKGGGIALGEGSPTSHV 183 Query: 189 AMLARARGIPMVVQLGT-IPDIGTTALLDGEGATLELDPNAEQVRTFEKRRDSHLKSRAS 247 AMLAR RG+PMVV LG + L+DG A + +DP+ E++ + D + +A Sbjct: 184 AMLARGRGVPMVVGLGRGWERLAGELLVDGTRAEVVVDPDPERIAAARREIDEFRRRKAE 243 Query: 248 ARAILRRPTASWRGERIKLFINIQRVEDLEHADAQYADGIGLMRTEFLLAGRSVPDEETQ 307 A A P + G + + +NI D+ A A DG+GL RTEFL A + DEE Q Sbjct: 244 AIARQHEPALTRDGTPVAVLVNISGPNDMAIAPASAGDGVGLARTEFLTAN-LLHDEEAQ 302 Query: 308 FQAYDAVLRWAGQRPVTIRTFDAGGDKPVPGFTLDGEANPFLGVRGLRLCLARPEIFAVQ 367 ++AY ++RWA RPVTIRT DAGGDKP+PG+T+DGE+NPFLG+RG+RL L PEIF VQ Sbjct: 303 YRAYAELMRWADGRPVTIRTLDAGGDKPIPGYTIDGESNPFLGLRGIRLSLRHPEIFRVQ 362 Query: 368 LRALARAAIRGNLKVMFPMVTSADELEAGRKLFADIVQRLQADGIAAMLPELGIMVEVPA 427 LRAL+RAA G LKVM PMVT+A EL+A R ++ L D +A P GIM+EVPA Sbjct: 363 LRALSRAAALGPLKVMLPMVTNAGELDAARGHLRAVLDDLAKDSLAFAEPAFGIMIEVPA 422 Query: 428 AALAVSSFKAAFFSIGSNDLAQYVLACDRSNGALAPLMDPLHPAVLELIARTAEHGRRAG 487 AALA F A FFSIG+NDL QY A R +A D LHP VL +IA R G Sbjct: 423 AALAAQDFAADFFSIGTNDLTQYATAASRDGAEVADYADVLHPGVLAMIAHVVRAARARG 482 Query: 488 ISVSLCGDMAGDPRCLPALLNCGLRELSVNASALAQIKHTIDRLSSGGSVG 538 + VS+CGD AGD +PALL G+R LSV+ L Q+K I L VG Sbjct: 483 VDVSICGDAAGDVSAIPALLRAGVRALSVSPGLLPQVKAAIRELDLARPVG 533