BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0026.p (451 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_772136.1| metabolite transport protein [Bradyrhizobium ja... 766 0.0 ref|YP_003908771.1| major facilitator superfamily protein [Burkh... 682 0.0 ref|ZP_08873222.1| major facilitator superfamily protein [Vermin... 635 e-180 ref|YP_001895715.1| major facilitator superfamily protein [Burkh... 630 e-178 ref|YP_995349.1| major facilitator superfamily transporter [Verm... 565 e-159 >ref|NP_772136.1| metabolite transport protein [Bradyrhizobium japonicum USDA 110] dbj|BAC50761.1| hypothetical metabolite transport protein [Bradyrhizobium japonicum USDA 110] Length = 452 Score = 766 bits (1979), Expect = 0.0, Method: Compositional matrix adjust. Identities = 388/448 (86%), Positives = 424/448 (94%) Query: 1 MSMISARIERLPFARFHVHLLLMGGLGYLFDAMDAAVLAFILPVLRTAWGLTNVETGVLG 60 MSMISARIERLPFARFH HLLLMGGLGY+FDAMDAAVLAFILPVLRTAW L++V+ GVLG Sbjct: 1 MSMISARIERLPFARFHKHLLLMGGLGYMFDAMDAAVLAFILPVLRTAWNLSSVQIGVLG 60 Query: 61 SSTFVGYLFGALLAGTLGDLIGRRAVMMSALALYSAASLVSAAVDSWPAFFAARVVAGMG 120 SST++G+LFGALLAGTLGDLIGRRAVMMSALALY AAS+VSA VD+WP+FFAARVVAGMG Sbjct: 61 SSTYIGFLFGALLAGTLGDLIGRRAVMMSALALYCAASIVSAMVDTWPSFFAARVVAGMG 120 Query: 121 TGAESAIIAPYLAEFVARRYRGAFTGALAGFFSFGFVAAALLGYFIVPAYENGWRIVLVI 180 TGAESAI+APYLAEFVARRYRG+FTGALAGFFSFGFVAAALLGYFIVPAYENGWRIVLVI Sbjct: 121 TGAESAIVAPYLAEFVARRYRGSFTGALAGFFSFGFVAAALLGYFIVPAYENGWRIVLVI 180 Query: 181 TAVPVVMLLWWRRSLPESPRWLESRGRAKEAEAVLDRIEASFAREGRALPAPIAEPAAPV 240 TAVPVVMLLWWRR+LPESPRWLESRGR KEAEA+LD IEA FAR+G LP P+ E AP Sbjct: 181 TAVPVVMLLWWRRALPESPRWLESRGREKEAEALLDTIEAGFARQGHVLPHPVVEAVAPQ 240 Query: 241 ASSGTLLSNFAALLAGRQARITTMTWIMWLSITFSYYSFFVWIPGLLVQNGMSITKSFGY 300 ++GTLL+NFAALLAGRQARIT MTWIMWL+ITFSYYSFFVWIPGLLVQNGMSITKSF Y Sbjct: 241 GATGTLLANFAALLAGRQARITIMTWIMWLAITFSYYSFFVWIPGLLVQNGMSITKSFAY 300 Query: 301 SLAMYCAQVPGYFTAAFFNERIGRQATIATYMLLGGASALGLAFAKSDAEIMTAGLLLSF 360 S+A+YCAQ+PGYF+AA+FNERIGRQATIATYM+LGGASALGLAFA+SD +IM AG+ LS Sbjct: 301 SIAIYCAQIPGYFSAAYFNERIGRQATIATYMVLGGASALGLAFAQSDQQIMAAGIFLSL 360 Query: 361 FMNGTYAGVYAYTAEVFPTSIRTTGAGLASAIGRIGAIAAPILVGYLYPNFGFAGVFGVT 420 FMNGTYAGVYAYTAEVFPT +RTTGAGLASAIGRIGAI +PILVGYLYPNFGFAGVFGVT Sbjct: 361 FMNGTYAGVYAYTAEVFPTPVRTTGAGLASAIGRIGAIVSPILVGYLYPNFGFAGVFGVT 420 Query: 421 TSVLLIGALTVVLMGVPTRGRSLEDIAS 448 T+VLL+GA+TVVLMGVPTRGRSLE+IA+ Sbjct: 421 TTVLLLGAVTVVLMGVPTRGRSLEEIAA 448 >ref|YP_003908771.1| major facilitator superfamily protein [Burkholderia sp. CCGE1003] gb|ADN59480.1| major facilitator superfamily MFS_1 [Burkholderia sp. CCGE1003] Length = 454 Score = 682 bits (1760), Expect = 0.0, Method: Compositional matrix adjust. Identities = 360/456 (78%), Positives = 400/456 (87%), Gaps = 7/456 (1%) Query: 1 MSMISARIERLPFARFHVHLLLMGGLGYLFDAMDAAVLAFILPVLRTAWGLTNVETGVLG 60 MS +SARIERLPF RFH HLLLMGGLGY FDAMDAA +AF+LPVLR W L++V+TGVLG Sbjct: 1 MSNVSARIERLPFGRFHGHLLLMGGLGYAFDAMDAAAVAFVLPVLRHDWSLSSVQTGVLG 60 Query: 61 SSTFVGYLFGALLAGTLGDLIGRRAVMMSALALYSAASLVSAAVDSWPAFFAARVVAGMG 120 S F+G+ FGALLAGTLGDLIGRR VMMSAL +Y AAS+VSA DSWP F AAR+VAGMG Sbjct: 61 SGNFIGFFFGALLAGTLGDLIGRRRVMMSALLIYCAASVVSAMTDSWPTFLAARIVAGMG 120 Query: 121 TGAESAIIAPYLAEFVARRYRGAFTGALAGFFSFGFVAAALLGYFIVPAYENGWRIVLVI 180 TGAESAIIAPYLAEFVA+RYRG FTGALAGFFSFGFVAAALLGYFIVPA++ GWR+VL+I Sbjct: 121 TGAESAIIAPYLAEFVAKRYRGIFTGALAGFFSFGFVAAALLGYFIVPAFDAGWRVVLLI 180 Query: 181 TAVPVVMLLWWRRSLPESPRWLESRGRAKEAEAVLDRIEASFAREGRALPAPIAEP---- 236 TAVP++MLLWWRRSLPESPRWLESRGR EA VLD IEASF G ALPAP EP Sbjct: 181 TAVPILMLLWWRRSLPESPRWLESRGRTDEATRVLDAIEASFTARGIALPAP--EPVEAR 238 Query: 237 AAPVASSGTLLSNFAALLAGRQARITTMTWIMWLSITFSYYSFFVWIPGLLVQNGMSITK 296 AAP +GTL+SN AL GRQARITTMTW+MWLSITFSYY+FF WIPGLLVQ+GMSIT+ Sbjct: 239 AAPALEAGTLISNLRALWTGRQARITTMTWLMWLSITFSYYAFFTWIPGLLVQHGMSITR 298 Query: 297 SFGYSLAMYCAQVPGYFTAAFFNERIGRQATIATYMLLGGASALGLAFAKSDAEIMTAGL 356 SFGYS+AMY AQVPGYF+A++FNERIGRQATIATYML G A ALG+AFA ++ +IM AG+ Sbjct: 299 SFGYSVAMYAAQVPGYFSASWFNERIGRQATIATYMLFGCACALGMAFAHTNGQIMAAGI 358 Query: 357 LLSFFMNGTYAGVYAYTAEVFPTSIRTTGAGLASAIGRIGAIAAPILVGYLYPNFGFAGV 416 LSFFMNGTYAGVYAYTAEVFPT++RTTGAG ASAIGRIGAI +PILVGYLYPNFGFAGV Sbjct: 359 GLSFFMNGTYAGVYAYTAEVFPTAVRTTGAGTASAIGRIGAIVSPILVGYLYPNFGFAGV 418 Query: 417 FGVTTSVLLIGALTVVLMGVPTRGRSLEDIAS-EAA 451 FGVTT VLL+GA+ VV+MGVPTRGRSLEDIA+ EAA Sbjct: 419 FGVTTVVLLLGAIAVVVMGVPTRGRSLEDIAAGEAA 454 >ref|ZP_08873222.1| major facilitator superfamily protein [Verminephrobacter sp. At4] Length = 455 Score = 635 bits (1637), Expect = e-180, Method: Compositional matrix adjust. Identities = 320/452 (70%), Positives = 376/452 (83%), Gaps = 3/452 (0%) Query: 1 MSMISARIERLPFARFHVHLLLMGGLGYLFDAMDAAVLAFILPVLRTAWGLTNVETGVLG 60 M+++SARIERLPF+ FH LLL+GGLGY FDAMDAAV+AF+LPVLRTAW L + + GVL Sbjct: 1 MTVLSARIERLPFSGFHRRLLLIGGLGYTFDAMDAAVIAFVLPVLRTAWNLDSGQVGVLA 60 Query: 61 SSTFVGYLFGALLAGTLGDLIGRRAVMMSALALYSAASLVSAAVDSWPAFFAARVVAGMG 120 S+ F+G+ FGALLAG+LGDLIGRR VMMSALA+Y AS SA V+ W +F RV+AG+G Sbjct: 61 SANFIGFFFGALLAGSLGDLIGRRMVMMSALAVYCVASFFSAMVNDWNSFLVLRVLAGLG 120 Query: 121 TGAESAIIAPYLAEFVARRYRGAFTGALAGFFSFGFVAAALLGYFIVPAYENGWRIVLVI 180 TGAES IIAPYL+EFVARRYRG FTGALAGFFSFGFVAAALLGY IVPA E+GWRIV++I Sbjct: 121 TGAESVIIAPYLSEFVARRYRGRFTGALAGFFSFGFVAAALLGYLIVPASEDGWRIVILI 180 Query: 181 TAVPVVMLLWWRRSLPESPRWLESRGRAKEAEAVLDRIEASFAREGRALPA--PIAEPAA 238 TA PV+MLLWWRR LPESPRWLES+GR EA+A++ +E + R G+ LPA P AA Sbjct: 181 TAAPVLMLLWWRRVLPESPRWLESQGRNAEADAIVAAMEHAIVRGGQPLPALDPHDVAAA 240 Query: 239 PVA-SSGTLLSNFAALLAGRQARITTMTWIMWLSITFSYYSFFVWIPGLLVQNGMSITKS 297 P + G LL N+A LL+ R T+MTW+MWLSITFSYY+FF WIP LLVQNGM++TKS Sbjct: 241 PPSRERGGLLKNYATLLSRRLLPTTSMTWLMWLSITFSYYTFFTWIPSLLVQNGMTMTKS 300 Query: 298 FGYSLAMYCAQVPGYFTAAFFNERIGRQATIATYMLLGGASALGLAFAKSDAEIMTAGLL 357 FGYSL MY AQVPGYF+AA+ N+RIGRQATI +YML+G +ALG+AFA+ DA+IM AG+L Sbjct: 301 FGYSLVMYLAQVPGYFSAAWLNDRIGRQATIVSYMLMGMLAALGMAFARGDAQIMAAGVL 360 Query: 358 LSFFMNGTYAGVYAYTAEVFPTSIRTTGAGLASAIGRIGAIAAPILVGYLYPNFGFAGVF 417 LSFF+NGTYAGVYAYT EVFPT +R TGAGLASAIGRIGAI APILVGY+YP GFAGVF Sbjct: 361 LSFFLNGTYAGVYAYTPEVFPTQVRATGAGLASAIGRIGAITAPILVGYVYPKLGFAGVF 420 Query: 418 GVTTSVLLIGALTVVLMGVPTRGRSLEDIASE 449 G TT+ LL+G+ V +MGVPTRGRSLEDIA+E Sbjct: 421 GTTTAALLMGSAAVWIMGVPTRGRSLEDIATE 452 >ref|YP_001895715.1| major facilitator superfamily protein [Burkholderia phytofirmans PsJN] gb|ACD16491.1| major facilitator superfamily MFS_1 [Burkholderia phytofirmans PsJN] Length = 456 Score = 630 bits (1626), Expect = e-178, Method: Compositional matrix adjust. Identities = 318/451 (70%), Positives = 374/451 (82%), Gaps = 3/451 (0%) Query: 1 MSMISARIERLPFARFHVHLLLMGGLGYLFDAMDAAVLAFILPVLRTAWGLTNVETGVLG 60 MSMI+ARIERLPF+ FH LLLMGGLGY FDAMDAAVLAF+LPVLRT W LT+V+TGVLG Sbjct: 1 MSMIAARIERLPFSGFHRRLLLMGGLGYTFDAMDAAVLAFLLPVLRTQWVLTSVQTGVLG 60 Query: 61 SSTFVGYLFGALLAGTLGDLIGRRAVMMSALALYSAASLVSAAVDSWPAFFAARVVAGMG 120 S TF+GY GA+LAG LGD+IGRR VMM AL +Y ASL SA + W F A R+VAG+G Sbjct: 61 SGTFMGYFVGAMLAGMLGDVIGRRKVMMYALVIYCVASLASALANDWGFFLATRIVAGLG 120 Query: 121 TGAESAIIAPYLAEFVARRYRGAFTGALAGFFSFGFVAAALLGYFIVPAYENGWRIVLVI 180 TGAESAI+AP+L+EFVARRYRG+FTG+LAGFFSFGFVAAALLGY ++P N WR V+VI Sbjct: 121 TGAESAIVAPFLSEFVARRYRGSFTGSLAGFFSFGFVAAALLGYLVIPLAPNAWRAVMVI 180 Query: 181 TAVPVVMLLWWRRSLPESPRWLESRGRAKEAEAVLDRIEASFAREGRAL-PAPIA-EPAA 238 TA+P+VMLLWWRR+LPESPRWLE+R R EA+A++ R+EA + G L P P A E A Sbjct: 181 TALPIVMLLWWRRALPESPRWLEARDRHAEADAIVSRMEAEVLKAGVQLEPLPAAREEAV 240 Query: 239 PVAS-SGTLLSNFAALLAGRQARITTMTWIMWLSITFSYYSFFVWIPGLLVQNGMSITKS 297 P+A+ ++++N AL A + ARIT MTW+MWLSITFSYY+FF WIPGLLVQNGM+IT+S Sbjct: 241 PLAAGRASIIANVKALWAAKLARITAMTWLMWLSITFSYYAFFTWIPGLLVQNGMTITRS 300 Query: 298 FGYSLAMYCAQVPGYFTAAFFNERIGRQATIATYMLLGGASALGLAFAKSDAEIMTAGLL 357 F YSL +Y AQ+PGYF+ A+ NE+IGRQATIA+YM+LGG SALGLA +D IM +G+L Sbjct: 301 FSYSLVIYIAQIPGYFSGAWLNEKIGRQATIASYMILGGISALGLALTGTDTGIMVSGIL 360 Query: 358 LSFFMNGTYAGVYAYTAEVFPTSIRTTGAGLASAIGRIGAIAAPILVGYLYPNFGFAGVF 417 LSFFMNGTYAGVYAYT EVFPT +R TG GLAS+IGR+GAIAAPILVGY+YP GFAGVF Sbjct: 361 LSFFMNGTYAGVYAYTPEVFPTDVRATGTGLASSIGRLGAIAAPILVGYVYPRLGFAGVF 420 Query: 418 GVTTSVLLIGALTVVLMGVPTRGRSLEDIAS 448 G TT VLLIGA V+LMGVPTRGRSLEDIA+ Sbjct: 421 GATTLVLLIGAAAVLLMGVPTRGRSLEDIAA 451 >ref|YP_995349.1| major facilitator superfamily transporter [Verminephrobacter eiseniae EF01-2] gb|ABM56331.1| major facilitator superfamily MFS_1 [Verminephrobacter eiseniae EF01-2] Length = 456 Score = 565 bits (1457), Expect = e-159, Method: Compositional matrix adjust. Identities = 283/452 (62%), Positives = 352/452 (77%), Gaps = 4/452 (0%) Query: 1 MSMISARIERLPFARFHVHLLLMGGLGYLFDAMDAAVLAFILPVLRTAWGLTNVETGVLG 60 MS +SARIERLPF ++H HLLL+GGLGY FDAMDAAV+AF+LPVL+++W L + G++ Sbjct: 1 MSAVSARIERLPFGKYHRHLLLLGGLGYAFDAMDAAVIAFVLPVLKSSWQLDGPQLGIVA 60 Query: 61 SSTFVGYLFGALLAGTLGDLIGRRAVMMSALALYSAASLVSAAVDSWPAFFAARVVAGMG 120 S+ F+G+ FGAL AG LGDLIGRR +MM AL +Y AS SA + W F A R++AG+G Sbjct: 61 SANFIGFFFGALSAGALGDLIGRRRIMMWALVVYCVASFFSAMSNEWGIFIALRIIAGIG 120 Query: 121 TGAESAIIAPYLAEFVARRYRGAFTGALAGFFSFGFVAAALLG-YFIVPAYENGWRIVLV 179 TGAESAIIAPYL+EFV RRYRG FTGALAGFFSFGF+AAALLG Y I P +E GWR+V++ Sbjct: 121 TGAESAIIAPYLSEFVPRRYRGIFTGALAGFFSFGFIAAALLGRYIITPDFEQGWRVVIL 180 Query: 180 ITAVPVVMLLWWRRSLPESPRWLESRGRAKEAEAVLDRIEASFAREGRALPAPIAEP--- 236 IT+ PV+MLLWWRR L ESPRWLES+GR EA V+ ++E RE LP AE Sbjct: 181 ITSTPVLMLLWWRRRLLESPRWLESQGRTDEALEVVAKMEQQVMREFPVLPQVDAEELDT 240 Query: 237 AAPVASSGTLLSNFAALLAGRQARITTMTWIMWLSITFSYYSFFVWIPGLLVQNGMSITK 296 PV +L + +A LL+ + IT MTW MWLSITF YYSFF WIP LL+QNGM++TK Sbjct: 241 PPPVVKQESLFAKYAFLLSKKLRPITIMTWCMWLSITFCYYSFFSWIPTLLIQNGMTLTK 300 Query: 297 SFGYSLAMYCAQVPGYFTAAFFNERIGRQATIATYMLLGGASALGLAFAKSDAEIMTAGL 356 SF Y+L MY AQ PGYF+AA+FNE+IGRQAT+ +YM+LG +A+G+A+A+SDA++MT G+ Sbjct: 301 SFTYALVMYIAQTPGYFSAAWFNEKIGRQATVVSYMVLGSLAAIGMAYARSDAQVMTMGI 360 Query: 357 LLSFFMNGTYAGVYAYTAEVFPTSIRTTGAGLASAIGRIGAIAAPILVGYLYPNFGFAGV 416 LLSFFMNG YAG+YAYT EVFPT +RT G GLASAIGRIG I API+VG+ Y +GF GV Sbjct: 361 LLSFFMNGAYAGIYAYTPEVFPTHVRTIGTGLASAIGRIGGITAPIMVGFTYETYGFIGV 420 Query: 417 FGVTTSVLLIGALTVVLMGVPTRGRSLEDIAS 448 FG TT +L +GA V++MG+PT+G+SLE IA+ Sbjct: 421 FGATTVILFLGAGAVLIMGIPTKGKSLEQIAA 452