BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0075.p (457 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value dbj|BAH86807.1| large subunit of 2,4-D oxygenase [Sphingomonas s... 572 e-161 ref|YP_004106978.1| aromatic-ring-hydroxylating dioxygenase subu... 560 e-157 dbj|BAD15048.1| hypothetical protein [Bradyrhizobium sp. RD5-C2] 558 e-157 dbj|BAB78521.1| 2,4-D oxygenase large subunit of oxygenase compo... 556 e-156 gb|AAB39767.1| 2,4,5-trichlorophenoxyacetic acid oxygenase 1 [Bu... 527 e-147 >dbj|BAH86807.1| large subunit of 2,4-D oxygenase [Sphingomonas sp. 58-1] Length = 448 Score = 572 bits (1473), Expect = e-161, Method: Compositional matrix adjust. Identities = 272/448 (60%), Positives = 329/448 (73%), Gaps = 10/448 (2%) Query: 13 SEISRLIDDRPGERIFEVDRRIFTDPAVFDAELRHIFEATWNFIGLESQIARPNDFVTTH 72 + ++ LIDDRP + +F VD +FTD A+F+AE+ IFE TW FIGLESQ+ +P+ F TT+ Sbjct: 5 ASLAALIDDRPEDGVFAVDSAVFTDEAIFEAEMTAIFEGTWIFIGLESQLPKPHSFFTTY 64 Query: 73 IGRHPILVMRSTEGKVGAFLNTCRHRGTIVCPFKQGRQKFHVCRYHGWAYDSGGRNISVT 132 IGR P++VM+ EG++G FLNTCRHRGT++CP K GRQKFH CRYHGW+YDSGG N+ VT Sbjct: 65 IGRQPVIVMKDGEGQIGCFLNTCRHRGTLLCPLKHGRQKFHTCRYHGWSYDSGGENVHVT 124 Query: 133 EEASGQYPPSFSNSNHDLLPVARLGSYRGFLFASLSADVPPLEQFLGDTRAFLDLIIDQS 192 EA+GQY SF+ ++H+L+ V R SYRG LFASL+ DVP L+ LGD + F+DL++DQS Sbjct: 125 AEATGQYAASFTEADHNLIRVPRFESYRGLLFASLNPDVPTLDDHLGDAKVFIDLVVDQS 184 Query: 193 DSGEVEFVPGASTYTFDANWKLQFENGLDYYHFATTHSSYVDILKKRNASAGPDAGFTVE 252 GE+E+VPG TYTF NWKLQFENGLD+YHF+TTHS+YVDIL +R + Sbjct: 185 PVGEIEYVPGEVTYTFKGNWKLQFENGLDFYHFSTTHSAYVDILAQRGKRGTLG---VLT 241 Query: 253 PEGEQEGQGSFSFDYGHAVNWSIKRTQLYGRPLGMDPARLDAVRQRIGSTRAKWMLRQRN 312 E E E QGSF+F YGHAVN+SI + L+ RPL ++ LDAVR+R+G RAKWMLRQRN Sbjct: 242 SEDEPEAQGSFNFHYGHAVNFSILQQSLFSRPLCLEQDALDAVRERVGPVRAKWMLRQRN 301 Query: 313 LTIFPNLQVIDILSAQVRTWRPLAPDKTEMVSHCLAPVGESAAARTLRIRNYEDFFNASG 372 LTI+PNLQ+IDI SAQ+RTWRPL+ +KTEM SHCL VGE AAR RIR YEDFFN G Sbjct: 302 LTIYPNLQIIDINSAQIRTWRPLSANKTEMTSHCLGIVGERPAARRFRIRGYEDFFNPPG 361 Query: 373 LASSDDNVMYEFCQTGYEATTAAPTQGYLRGVAEVGHGESAHTRELGITASEWS------ 426 LASSDDN MYE CQ GY A A P QGY RG+A A ELGIT E S Sbjct: 362 LASSDDNAMYELCQAGY-AAQAGPRQGYGRGLAGTPGAGDAQAAELGITPVESSMGVGKA 420 Query: 427 FGPTSFGGETNFHPGYREWRRLLQHAAG 454 FG +FGGETNF PGYREW+RLLQ G Sbjct: 421 FGNEAFGGETNFFPGYREWKRLLQRGNG 448 >ref|YP_004106978.1| aromatic-ring-hydroxylating dioxygenase subunit alpha-like protein [Rhodopseudomonas palustris DX-1] gb|ADU42245.1| Aromatic-ring-hydroxylating dioxygenase, alpha subunit-like protein [Rhodopseudomonas palustris DX-1] Length = 471 Score = 560 bits (1444), Expect = e-157, Method: Compositional matrix adjust. Identities = 264/446 (59%), Positives = 326/446 (73%), Gaps = 9/446 (2%) Query: 15 ISRLIDDRPGERIFEVDRRIFTDPAVFDAELRHIFEATWNFIGLESQIARPNDFVTTHIG 74 +S+L+DDRP + IF VDR +FTD +F+ EL H+FE TW F+GLESQ+ +PNDFVTT+IG Sbjct: 29 VSKLLDDRPDDGIFRVDRTVFTDQDIFERELTHVFEGTWVFVGLESQLRKPNDFVTTYIG 88 Query: 75 RHPILVMRSTEGKVGAFLNTCRHRGTIVCPFKQGRQKFHVCRYHGWAYDSGGRNISVTEE 134 RHP+L+ + G++ F NTCRHRG IVCPFK+G QKFHVCRYHGW+YDS G+N+S+T+E Sbjct: 89 RHPVLITKDGTGEIRCFFNTCRHRGAIVCPFKKGNQKFHVCRYHGWSYDSAGKNVSMTDE 148 Query: 135 ASGQYPPSFSNSNHDLLPVARLGSYRGFLFASLSADVPPLEQFLGDTRAFLDLIIDQSDS 194 GQYPPSF +H L PV ++ SYRG LFAS+S DVP LE+ LGD RAF+DL++DQ Sbjct: 149 KDGQYPPSFLADDHGLKPVPKIASYRGLLFASVSPDVPTLEEHLGDARAFIDLVVDQGIE 208 Query: 195 GEVEFVPGASTYTFDANWKLQFENGLDYYHFATTHSSYVDILKKRNASAGPDAGFTVEP- 253 G +EFV G +YTFDANWKLQFENGLDYYHFA+THSSY+D+LKKR GP V+P Sbjct: 209 G-LEFVDGNVSYTFDANWKLQFENGLDYYHFASTHSSYIDVLKKREVRRGP---LEVKPW 264 Query: 254 ---EGEQEGQGSFSFDYGHAVNWSIKRTQLYG-RPLGMDPARLDAVRQRIGSTRAKWMLR 309 E + + QGSFS GHA+ WSI +++Y RPL D L V+ + + KWM R Sbjct: 265 NPTETDPDAQGSFSLARGHAMMWSIHASRVYKLRPLNQDGKLLSTVQGQTREDKLKWMFR 324 Query: 310 QRNLTIFPNLQVIDILSAQVRTWRPLAPDKTEMVSHCLAPVGESAAARTLRIRNYEDFFN 369 QRNLTI+PNLQ++DI + Q+RTWRPLAPDKTE+ SHCLAPVGES AR +RIR YEDFFN Sbjct: 325 QRNLTIYPNLQIVDIGTLQLRTWRPLAPDKTEITSHCLAPVGESDEARRVRIRLYEDFFN 384 Query: 370 ASGLASSDDNVMYEFCQTGYEATTAAPTQGYLRGVAEVGHGESAHTRELGITASEWSFGP 429 SGLA+SDDNVMYE CQTGY A A PTQGY RG+ ++ EL ++ W++G Sbjct: 385 PSGLATSDDNVMYELCQTGYGARFAGPTQGYARGLRPKSGEALNYSSELQVSPDRWTYGA 444 Query: 430 TSFGGETNFHPGYREWRRLLQHAAGE 455 FG ET FH GYREW RL+ E Sbjct: 445 PLFGDETCFHSGYREWLRLMTRDPSE 470 >dbj|BAD15048.1| hypothetical protein [Bradyrhizobium sp. RD5-C2] Length = 448 Score = 558 bits (1439), Expect = e-157, Method: Compositional matrix adjust. Identities = 264/445 (59%), Positives = 329/445 (73%), Gaps = 10/445 (2%) Query: 12 LSEISRLIDDRPGERIFEVDRRIFTDPAVFDAELRHIFEATWNFIGLESQIARPNDFVTT 71 + ++ L++DRP + IF V+ +FTD A+F AE+ IFE TW ++GL+SQ+ P+ F TT Sbjct: 3 VERLNTLVEDRPEDDIFAVNSAVFTDEAIFAAEMAAIFEGTWIYVGLDSQLPEPHSFFTT 62 Query: 72 HIGRHPILVMRSTEGKVGAFLNTCRHRGTIVCPFKQGRQKFHVCRYHGWAYDSGGRNISV 131 +IGR P++VM+ +G+VG FLNTCRHRGT++CP+K G QK HVCRYHGW+Y++GG N+ V Sbjct: 63 YIGRQPVIVMKDGDGQVGCFLNTCRHRGTLLCPYKHGCQKIHVCRYHGWSYNTGGENVFV 122 Query: 132 TEEASGQYPPSFSNSNHDLLPVARLGSYRGFLFASLSADVPPLEQFLGDTRAFLDLIIDQ 191 T EA+GQY SF+ ++HDL+ V R SYRG LFASL+ADVP L + LGD + F+DL++DQ Sbjct: 123 TAEATGQYAASFTEADHDLVKVPRFESYRGLLFASLNADVPSLSEHLGDAKVFIDLVVDQ 182 Query: 192 SDSGEVEFVPGASTYTFDANWKLQFENGLDYYHFATTHSSYVDILKKRNASAGPDAGFTV 251 S G++E+VPG +TYTF+ NWKLQFENGLD+YHF++THSSYVDIL +R A G + Sbjct: 183 SPVGKIEYVPGETTYTFNGNWKLQFENGLDFYHFSSTHSSYVDILAER-AKRGTIG--VL 239 Query: 252 EPEGEQEGQGSFSFDYGHAVNWSIKRTQLYGRPLGMDPARLDAVRQRIGSTRAKWMLRQR 311 E E E QGSF+F GHAVNWSI ++ RPL ++ LDAVR R+G+TRAKWMLRQR Sbjct: 240 PSEDEPEAQGSFNFHRGHAVNWSILSQSIFSRPLCLEQDALDAVRARVGATRAKWMLRQR 299 Query: 312 NLTIFPNLQVIDILSAQVRTWRPLAPDKTEMVSHCLAPVGESAAARTLRIRNYEDFFNAS 371 NLTI+PNLQ+IDI SAQ+RTWRPL+ KTEM SHCL VGESAAAR RIR YEDFFN Sbjct: 300 NLTIYPNLQIIDINSAQIRTWRPLSAHKTEMTSHCLGIVGESAAARRFRIRGYEDFFNPP 359 Query: 372 GLASSDDNVMYEFCQTGYEATTAAPTQGYLRGVAEVGHGESAHTRELGITASE------W 425 GLASSDDN MYE CQ GY A A P QGY RG+A EL I+ E Sbjct: 360 GLASSDDNAMYELCQAGY-AAEAGPRQGYARGMASRPGAAQKQADELNISPVESAIGVGK 418 Query: 426 SFGPTSFGGETNFHPGYREWRRLLQ 450 +FG +FGGETNF PGYREW+RLLQ Sbjct: 419 AFGAKAFGGETNFFPGYREWKRLLQ 443 >dbj|BAB78521.1| 2,4-D oxygenase large subunit of oxygenase component [Bradyrhizobium sp. HW13] Length = 448 Score = 556 bits (1432), Expect = e-156, Method: Compositional matrix adjust. Identities = 264/445 (59%), Positives = 328/445 (73%), Gaps = 10/445 (2%) Query: 12 LSEISRLIDDRPGERIFEVDRRIFTDPAVFDAELRHIFEATWNFIGLESQIARPNDFVTT 71 + ++ L++DRP + IF V+ +FTD AVF AE+ IFE TW ++GL+SQ+ P+ F TT Sbjct: 3 VERLNTLVEDRPEDDIFAVNSAVFTDEAVFAAEMAAIFEGTWIYVGLDSQLPEPHSFFTT 62 Query: 72 HIGRHPILVMRSTEGKVGAFLNTCRHRGTIVCPFKQGRQKFHVCRYHGWAYDSGGRNISV 131 +IGR P++VM+ +G+VG FLNTCRHRGT++CP+K G QK HVCRYHGW+Y++GG N+ V Sbjct: 63 YIGRQPVIVMKDGDGQVGCFLNTCRHRGTLLCPYKHGCQKIHVCRYHGWSYNTGGENVFV 122 Query: 132 TEEASGQYPPSFSNSNHDLLPVARLGSYRGFLFASLSADVPPLEQFLGDTRAFLDLIIDQ 191 T EA+GQY SF+ ++H L+ V R SYRG LFASL+ADVP L + LGD + F+DL++DQ Sbjct: 123 TAEATGQYAASFTEADHHLVKVPRFESYRGLLFASLNADVPSLSEHLGDAKVFIDLVVDQ 182 Query: 192 SDSGEVEFVPGASTYTFDANWKLQFENGLDYYHFATTHSSYVDILKKRNASAGPDAGFTV 251 S G++E+VPG +TYTF+ NWKLQFENGLD+YHF++THSSYVDIL +R A G + Sbjct: 183 SPVGKIEYVPGETTYTFNGNWKLQFENGLDFYHFSSTHSSYVDILAER-AKRGTIG--VL 239 Query: 252 EPEGEQEGQGSFSFDYGHAVNWSIKRTQLYGRPLGMDPARLDAVRQRIGSTRAKWMLRQR 311 E E E QGSF+F GHAVNWSI ++ RPL ++ LDAVR R+G+TRAKWMLRQR Sbjct: 240 PSEDEPEAQGSFNFHRGHAVNWSILSQSIFSRPLCLEQDALDAVRARVGATRAKWMLRQR 299 Query: 312 NLTIFPNLQVIDILSAQVRTWRPLAPDKTEMVSHCLAPVGESAAARTLRIRNYEDFFNAS 371 NLTI+PNLQ+IDI SAQ+RTWRPL+ KTEM SHCL VGESAAAR RIR YEDFFN Sbjct: 300 NLTIYPNLQIIDINSAQIRTWRPLSAHKTEMTSHCLGIVGESAAARRFRIRGYEDFFNPP 359 Query: 372 GLASSDDNVMYEFCQTGYEATTAAPTQGYLRGVAEVGHGESAHTRELGITASE------W 425 GLASSDDN MYE CQ GY A A P QGY RG+A EL I+ E Sbjct: 360 GLASSDDNAMYELCQAGY-AAEAGPRQGYARGMASRPGAAQKQADELNISPVESAIGVGK 418 Query: 426 SFGPTSFGGETNFHPGYREWRRLLQ 450 +FG +FGGETNF PGYREW+RLLQ Sbjct: 419 AFGAKAFGGETNFFPGYREWKRLLQ 443 >gb|AAB39767.1| 2,4,5-trichlorophenoxyacetic acid oxygenase 1 [Burkholderia cepacia] Length = 467 Score = 527 bits (1358), Expect = e-147, Method: Compositional matrix adjust. Identities = 253/440 (57%), Positives = 317/440 (72%), Gaps = 10/440 (2%) Query: 18 LIDDRPGERIFEVDRRIFTDPAVFDAELRHIFEATWNFIGLESQIARPNDFVTTHIGRHP 77 L+DDR + IF V R F DP +F+ E+ IFE+TW FIGLESQ+A+P+DF+T IGR P Sbjct: 21 LVDDRATDGIFRVHREAFLDPRIFELEMSRIFESTWVFIGLESQVAKPHDFITADIGRQP 80 Query: 78 ILVMRSTEGKVGAFLNTCRHRGTIVCPFKQGRQKFHVCRYHGWAYDSGGRNISVTEEASG 137 +++ R+ EGK+ FLN+CRHRG ++CPF +G QKFHVCRYHGW+YDS GRNI++T++ G Sbjct: 81 VILTRNAEGKLACFLNSCRHRGALLCPFSKGNQKFHVCRYHGWSYDSSGRNIAITDQKDG 140 Query: 138 QYPPSFSNSNHDLLPVARLGSYRGFLFASLSADVPPLEQFLGDTRAFLDLIIDQSDSGEV 197 QYP +F NHDL+ VARL SYRGF+FASLS DVP L++ LG FLDL+ DQ+ +G + Sbjct: 141 QYPSAFDKENHDLVSVARLESYRGFVFASLSPDVPTLQEHLGGASVFLDLVADQAPNG-L 199 Query: 198 EFVPGASTYTFDANWKLQFENGLDYYHFATTHSSYVDILKKRNASA-GPDA---GFTVEP 253 E+V G YTFDANWKLQFENGLD+YHF +THSSYVDILK R A PDA G + Sbjct: 200 EYVSGTVDYTFDANWKLQFENGLDFYHFGSTHSSYVDILKLRAQRAVAPDATQTGVKLAS 259 Query: 254 EGEQEGQGSFSFDYGHAVNWSIKRTQLYGRPLGMDPARLDAVRQRIGSTRAKWMLRQRNL 313 E ++GQG+FSF GH+V WS + R +++A + R G R KWM+RQRNL Sbjct: 260 EPNEQGQGTFSFPRGHSVMWSTFQPGQEQRLSDGKGLQMNA-KARAGEVRWKWMMRQRNL 318 Query: 314 TIFPNLQVIDI----LSAQVRTWRPLAPDKTEMVSHCLAPVGESAAARTLRIRNYEDFFN 369 TIFPNLQ+ID+ LS Q+RTW+PLA DKT M+SHCLAP+GES R RIR YEDFFN Sbjct: 319 TIFPNLQIIDLQSMQLSLQLRTWQPLAVDKTRMLSHCLAPIGESFDERRSRIRRYEDFFN 378 Query: 370 ASGLASSDDNVMYEFCQTGYEATTAAPTQGYLRGVAEVGHGESAHTRELGITASEWSFGP 429 SGLA+SDDNVMYEFCQ+GY A A TQGY+RG+ + + + ELG+ E +G Sbjct: 379 PSGLATSDDNVMYEFCQSGYRAIAAGATQGYVRGLGKPTENQRDYAGELGLDNCESVYGS 438 Query: 430 TSFGGETNFHPGYREWRRLL 449 +FG ET FH GYREW+RLL Sbjct: 439 MAFGNETCFHAGYREWQRLL 458