BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0074.p (171 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_004106979.1| aromatic-ring-hydroxylating dioxygenase subu... 182 2e-44 gb|AAB39768.1| 2,4,5-trichlorophenoxyacetic acid oxygenase 2 [Bu... 176 1e-42 dbj|BAB78522.1| 2,4-D oxygenase small subunit of oxygenase compo... 169 1e-40 dbj|BAH86808.1| small subunit of 2,4-D oxygenase [Sphingomonas s... 164 5e-39 ref|YP_496955.1| aromatic-ring-hydroxylating dioxygenase subunit... 140 5e-32 >ref|YP_004106979.1| aromatic-ring-hydroxylating dioxygenase subunit beta [Rhodopseudomonas palustris DX-1] gb|ADU42246.1| aromatic-ring-hydroxylating dioxygenase beta subunit [Rhodopseudomonas palustris DX-1] Length = 166 Score = 182 bits (461), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 83/162 (51%), Positives = 110/162 (67%) Query: 10 DTHAVAAATIFREARLLDTGEWPDWVAMYSEDAVFWVPAWLDEYTTTNDPDTQVSLFYHD 69 D +A +F EARLLD ++ DWV +Y++DA+FWVPAW DEY TT++P+ ++S+ YH+ Sbjct: 5 DYLKIATRVLFTEARLLDQKKFRDWVKLYADDAIFWVPAWKDEYETTSNPNRELSMIYHE 64 Query: 70 ARLGLEERIARIESRKSITALPLPRTVHQISNLEASETGPDEITCHSVFSVHVYDPRVAK 129 GL +RI RI+SRKSITA+PLPRTVH +N+ D I ++ F+VH YDPR K Sbjct: 65 NSYGLSDRIDRIQSRKSITAMPLPRTVHSYTNIMVDAASTDSIEGNACFTVHTYDPRACK 124 Query: 130 EHLRHGRYEHTLRRDGETWKIARKVITLVNDRVPTVLDFYSI 171 H GR E LRR GE W I K I+L ND++ T LDFYS+ Sbjct: 125 SHTNFGRLEFRLRRLGEAWLIGFKKISLTNDQMATALDFYSV 166 >gb|AAB39768.1| 2,4,5-trichlorophenoxyacetic acid oxygenase 2 [Burkholderia cepacia] Length = 166 Score = 176 bits (446), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 83/166 (50%), Positives = 118/166 (71%), Gaps = 2/166 (1%) Query: 8 LTDTHAVAAAT--IFREARLLDTGEWPDWVAMYSEDAVFWVPAWLDEYTTTNDPDTQVSL 65 + D +AVA AT + RE LD +W +W+ +Y+EDA++WVPAW +EY T +PDT++SL Sbjct: 1 MLDQNAVAIATNVLCREGFCLDRRDWNEWLELYAEDAIYWVPAWRNEYEETENPDTEISL 60 Query: 66 FYHDARLGLEERIARIESRKSITALPLPRTVHQISNLEASETGPDEITCHSVFSVHVYDP 125 YH++RLGLEER+ RI+SRKS+TA+PLPRT H +SN+ S + I + + VHVYD Sbjct: 61 IYHESRLGLEERVMRIQSRKSVTAMPLPRTTHFVSNIVGSTKDQEIIEAQASWMVHVYDV 120 Query: 126 RVAKEHLRHGRYEHTLRRDGETWKIARKVITLVNDRVPTVLDFYSI 171 A++H+ G E L+R G++W I+RK I L NDRVPTV+DF ++ Sbjct: 121 HTARKHMHFGWCELQLQRRGDSWLISRKKIHLQNDRVPTVIDFITL 166 >dbj|BAB78522.1| 2,4-D oxygenase small subunit of oxygenase component [Bradyrhizobium sp. HW13] Length = 177 Score = 169 bits (428), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 82/160 (51%), Positives = 108/160 (67%), Gaps = 1/160 (0%) Query: 13 AVAAATIFREARLLDTGEWPDWVAMYSEDAVFWVPAWLDEYTTTNDPDTQVSLFYHDARL 72 AVA+ I EA LD+ W W+A+YS DA FW+PAWLDE ++DP+TQVSL YH++RL Sbjct: 18 AVASDLILTEAHELDSRNWAAWLALYSVDAKFWMPAWLDENAQSDDPNTQVSLIYHESRL 77 Query: 73 GLEERIARIESRKSITALPLPRTVHQISNLEASETGPDEITCHSVFSVHVYDPRVAKEHL 132 LEER+ R+ S KSIT +PLPRT+H I + D I S +VHVYDPR + H+ Sbjct: 78 ELEERVDRVNSLKSITTMPLPRTIHVIGGIRLLAHSEDRIQASSSANVHVYDPRTSDHHI 137 Query: 133 RHGRYEHTLRRDGET-WKIARKVITLVNDRVPTVLDFYSI 171 RY HTL R G+ W I K I+L+NDR+P+V+DFY++ Sbjct: 138 AAVRYSHTLSRTGDGPWLITCKTISLINDRMPSVVDFYTV 177 >dbj|BAH86808.1| small subunit of 2,4-D oxygenase [Sphingomonas sp. 58-1] Length = 177 Score = 164 bits (414), Expect = 5e-39, Method: Compositional matrix adjust. Identities = 78/160 (48%), Positives = 108/160 (67%), Gaps = 1/160 (0%) Query: 13 AVAAATIFREARLLDTGEWPDWVAMYSEDAVFWVPAWLDEYTTTNDPDTQVSLFYHDARL 72 A+A+ +F+EA LD+ +W W+A+Y EDA FW+PAWLDEY T++PDT+VSL YH+ R Sbjct: 18 ALASDLLFQEADALDSQDWTGWLALYHEDARFWMPAWLDEYRNTSNPDTEVSLIYHEMRW 77 Query: 73 GLEERIARIESRKSITALPLPRTVHQISNLEASETGPDEITCHSVFSVHVYDPRVAKEHL 132 LEER+ R+ SRKS+T LPLPRT+H I + + + S +VHVYDPR H+ Sbjct: 78 ELEERVHRLGSRKSVTXLPLPRTLHMIGGSRVTAHSENRLDVRSHATVHVYDPRTFDHHV 137 Query: 133 RHGRYEHTL-RRDGETWKIARKVITLVNDRVPTVLDFYSI 171 RY H+ R + W IA K I L+NDR+P+V+DFY++ Sbjct: 138 AAVRYSHSFVREEAGPWLIASKTIILINDRMPSVVDFYTV 177 >ref|YP_496955.1| aromatic-ring-hydroxylating dioxygenase subunit beta [Novosphingobium aromaticivorans DSM 12444] gb|ABD26121.1| aromatic-ring-hydroxylating dioxygenase, beta subunit [Novosphingobium aromaticivorans DSM 12444] Length = 166 Score = 140 bits (354), Expect = 5e-32, Method: Compositional matrix adjust. Identities = 67/161 (41%), Positives = 99/161 (61%) Query: 11 THAVAAATIFREARLLDTGEWPDWVAMYSEDAVFWVPAWLDEYTTTNDPDTQVSLFYHDA 70 + A A +FREA L+DTG + +W+ +++ D FW+PAW DE TTT DPD ++SL Y+ Sbjct: 6 SRAEAEELLFREAVLIDTGRFAEWLDLFTPDVEFWMPAWRDETTTTEDPDRELSLIYYKG 65 Query: 71 RLGLEERIARIESRKSITALPLPRTVHQISNLEASETGPDEITCHSVFSVHVYDPRVAKE 130 R LE+R+ R+ S S + PLPR VHQ++N+ D + F H +D R+ + Sbjct: 66 RRNLEDRVMRLTSGLSTASTPLPRVVHQVTNVLVDAEEEDAARVSAAFVCHRFDVRMNRA 125 Query: 131 HLRHGRYEHTLRRDGETWKIARKVITLVNDRVPTVLDFYSI 171 GRY++ L R + W+IARK I L+ND +PTV+D +I Sbjct: 126 DCFFGRYDYRLVRGEDGWRIARKKIVLLNDTIPTVVDINAI 166