BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0063.p (313 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_578197.1| secretion protein HlyD [Nitrobacter hamburgensi... 448 e-124 ref|ZP_01048270.1| hypothetical protein NB311A_08834 [Nitrobacte... 441 e-122 ref|ZP_07026834.1| efflux transporter, RND family, MFP subunit [... 427 e-118 ref|YP_003757061.1| secretion protein HlyD [Hyphomicrobium denit... 411 e-113 ref|YP_001768169.1| secretion protein HlyD family protein [Methy... 405 e-111 >ref|YP_578197.1| secretion protein HlyD [Nitrobacter hamburgensis X14] gb|ABE63737.1| secretion protein HlyD [Nitrobacter hamburgensis X14] Length = 313 Score = 448 bits (1153), Expect = e-124, Method: Compositional matrix adjust. Identities = 219/313 (69%), Positives = 260/313 (83%) Query: 1 MGVAIINTYLVLLFLLVHFKIVRFNLFWKLSPFIVLILVMFGLLVPMNWGAPQGSALVVR 60 M +A++ YL LLF LV ++RFN FWK SP IVL+L+ L +PM WGAP+G ALV R Sbjct: 1 MMIALMVLYLALLFTLVWLGLIRFNAFWKTSPLIVLLLLNVLLFIPMGWGAPEGKALVYR 60 Query: 61 NAVSIVPSVAGEVTEVPVVANAPLKAGDVLFKIDPTPYDAQVKAITAQLKLSKTRLTQMT 120 N+VSIVP+VAGEV +VPV N PLK GDVLFKIDPTPYDAQ+KAI AQLKLS TRL QM Sbjct: 61 NSVSIVPNVAGEVVDVPVAPNTPLKQGDVLFKIDPTPYDAQLKAIEAQLKLSTTRLGQMA 120 Query: 121 TLFERDAGRGFDVEQRQSEVDQLSGQLEAAQWNLDKTVVTAPADGYVTNVALRKGARVTN 180 TL+ER+AGRGFDVEQRQSEVDQ QLE A+WNLDKTVV APADGYVTN+ALRKGARV N Sbjct: 121 TLYEREAGRGFDVEQRQSEVDQFKAQLENARWNLDKTVVRAPADGYVTNLALRKGARVGN 180 Query: 181 LPLSPVMAFIDTSNTIIGVQINQIDARYVAPGQEVEVTFKFAPGQIYSGKVESVLQAIAT 240 L +SPVMAFIDTS T + V+I+QI+ARYVAPGQ+VE+ FKFAPG++++GKVES+LQA+AT Sbjct: 181 LSVSPVMAFIDTSTTNVAVEIDQINARYVAPGQDVEIAFKFAPGRVFTGKVESILQAVAT 240 Query: 241 GQTQTSGTAVAPKAIEAAPFVVRVKLDDATFANRLPAGSAGTAAIYTEHLKPTHVVRRVL 300 GQ Q SG AV PK I PFVVR+ +DD FA LPAG+ GTAAI+T+H+K +HV+RRVL Sbjct: 241 GQAQVSGAAVTPKEITTVPFVVRIAMDDPDFARTLPAGATGTAAIFTDHVKISHVIRRVL 300 Query: 301 LRQVAIINYVNPF 313 LRQVAI++Y+NPF Sbjct: 301 LRQVAILDYINPF 313 >ref|ZP_01048270.1| hypothetical protein NB311A_08834 [Nitrobacter sp. Nb-311A] gb|EAQ33761.1| hypothetical protein NB311A_08834 [Nitrobacter sp. Nb-311A] Length = 313 Score = 441 bits (1135), Expect = e-122, Method: Compositional matrix adjust. Identities = 215/293 (73%), Positives = 259/293 (88%) Query: 21 IVRFNLFWKLSPFIVLILVMFGLLVPMNWGAPQGSALVVRNAVSIVPSVAGEVTEVPVVA 80 IVRFNLFWK+SP ++L L++FGL +PM WGAPQG ALVVR +V+IVP+VAGEV +VPV Sbjct: 21 IVRFNLFWKVSPLLMLFLLLFGLFIPMGWGAPQGPALVVRQSVAIVPNVAGEVVDVPVAV 80 Query: 81 NAPLKAGDVLFKIDPTPYDAQVKAITAQLKLSKTRLTQMTTLFERDAGRGFDVEQRQSEV 140 N PLK GDVLFKIDP PYDA+V+ I+A+LKL+ TRL+QMTTL+ER+AGR FDVEQRQSEV Sbjct: 81 NTPLKTGDVLFKIDPAPYDAKVQTISAKLKLASTRLSQMTTLYEREAGRRFDVEQRQSEV 140 Query: 141 DQLSGQLEAAQWNLDKTVVTAPADGYVTNVALRKGARVTNLPLSPVMAFIDTSNTIIGVQ 200 DQL GQL+AAQWNL+KT+V AP++GYVTNVALR GARV+N+PLSPVMAFIDT++T+IGV+ Sbjct: 141 DQLKGQLQAAQWNLEKTIVRAPSEGYVTNVALRTGARVSNIPLSPVMAFIDTTDTLIGVE 200 Query: 201 INQIDARYVAPGQEVEVTFKFAPGQIYSGKVESVLQAIATGQTQTSGTAVAPKAIEAAPF 260 ++QI AR++ P Q VEVTFKF PGQIY+GKVE+VLQAIATGQ QTSG AVAPK+I+A PF Sbjct: 201 VDQIYARFLKPSQPVEVTFKFRPGQIYTGKVETVLQAIATGQVQTSGAAVAPKSIQANPF 260 Query: 261 VVRVKLDDATFANRLPAGSAGTAAIYTEHLKPTHVVRRVLLRQVAIINYVNPF 313 VVRV+LDDA FA LPAGS G AAI+T+ +K THV+R+VLLRQ+AI NYVNPF Sbjct: 261 VVRVRLDDAAFAASLPAGSIGVAAIFTDRVKVTHVIRKVLLRQIAITNYVNPF 313 >ref|ZP_07026834.1| efflux transporter, RND family, MFP subunit [Afipia sp. 1NLS2] gb|EFI51677.1| efflux transporter, RND family, MFP subunit [Afipia sp. 1NLS2] Length = 429 Score = 427 bits (1099), Expect = e-118, Method: Compositional matrix adjust. Identities = 201/283 (71%), Positives = 237/283 (83%) Query: 21 IVRFNLFWKLSPFIVLILVMFGLLVPMNWGAPQGSALVVRNAVSIVPSVAGEVTEVPVVA 80 IVRFNLFWK+SP +VL L+MFGL +PM WGAP G LVVR +++IVP VAGEV +VPV Sbjct: 39 IVRFNLFWKVSPLLVLCLLMFGLFIPMGWGAPSGPTLVVRQSIAIVPDVAGEVIDVPVEP 98 Query: 81 NAPLKAGDVLFKIDPTPYDAQVKAITAQLKLSKTRLTQMTTLFERDAGRGFDVEQRQSEV 140 N LKAGDVLFKIDP PY AQV A+ AQLKLS+ RL QMT L ERD+GR FDV+QRQSEV Sbjct: 99 NTRLKAGDVLFKIDPVPYQAQVGALEAQLKLSELRLAQMTQLLERDSGRAFDVQQRQSEV 158 Query: 141 DQLSGQLEAAQWNLDKTVVTAPADGYVTNVALRKGARVTNLPLSPVMAFIDTSNTIIGVQ 200 DQL QLE A+WNLDKTVV AP+DGYVTNVALRKGARV+NLPLSPVMAFIDTS+T+IG++ Sbjct: 159 DQLKAQLEGAKWNLDKTVVRAPSDGYVTNVALRKGARVSNLPLSPVMAFIDTSDTLIGIE 218 Query: 201 INQIDARYVAPGQEVEVTFKFAPGQIYSGKVESVLQAIATGQTQTSGTAVAPKAIEAAPF 260 I+QI ARY+ GQ VEVTFKF PGQ+Y+GKVE+VLQAI+ GQ Q SGTAV PK I+A P Sbjct: 219 IDQIYARYLKAGQPVEVTFKFRPGQVYTGKVETVLQAISAGQVQVSGTAVTPKGIQAGPL 278 Query: 261 VVRVKLDDATFANRLPAGSAGTAAIYTEHLKPTHVVRRVLLRQ 303 VVR++L+DA A LPAGSAG AAI+T+H+K H++R+VLLR+ Sbjct: 279 VVRIRLNDAAVAEDLPAGSAGAAAIFTDHIKSAHIIRKVLLRR 321 >ref|YP_003757061.1| secretion protein HlyD [Hyphomicrobium denitrificans ATCC 51888] gb|ADJ24740.1| secretion protein HlyD family protein [Hyphomicrobium denitrificans ATCC 51888] Length = 313 Score = 411 bits (1057), Expect = e-113, Method: Compositional matrix adjust. Identities = 192/294 (65%), Positives = 240/294 (81%) Query: 20 KIVRFNLFWKLSPFIVLILVMFGLLVPMNWGAPQGSALVVRNAVSIVPSVAGEVTEVPVV 79 K+V FNLFWK+SP +V++ ++ GL +PM WGAP G +V R++V+I P VAGEV +VPV Sbjct: 20 KVVPFNLFWKISPVLVMLALLVGLFIPMGWGAPSGQLVVGRHSVAITPDVAGEVVDVPVQ 79 Query: 80 ANAPLKAGDVLFKIDPTPYDAQVKAITAQLKLSKTRLTQMTTLFERDAGRGFDVEQRQSE 139 NA LK GD+LF+IDP P++AQVKA+ AQLKLS RL QMT L E+ AGR FDVEQR++E Sbjct: 80 PNAQLKQGDILFRIDPVPFEAQVKALEAQLKLSTQRLGQMTELVEKAAGRQFDVEQRRAE 139 Query: 140 VDQLSGQLEAAQWNLDKTVVTAPADGYVTNVALRKGARVTNLPLSPVMAFIDTSNTIIGV 199 VDQL+ Q+E A+WNL+KT+V APADGYVTN+ALRKGARV +LPL+PVMAFIDTS+TI+ Sbjct: 140 VDQLNAQIEGAKWNLEKTIVRAPADGYVTNLALRKGARVASLPLAPVMAFIDTSDTIVAA 199 Query: 200 QINQIDARYVAPGQEVEVTFKFAPGQIYSGKVESVLQAIATGQTQTSGTAVAPKAIEAAP 259 +I QI ARYV PGQ EVTFKF PGQ+++GKVE+VLQA++TGQTQ SG AV PK + +AP Sbjct: 200 EIPQIYARYVMPGQSAEVTFKFLPGQVFTGKVETVLQAVSTGQTQVSGLAVTPKQVASAP 259 Query: 260 FVVRVKLDDATFANRLPAGSAGTAAIYTEHLKPTHVVRRVLLRQVAIINYVNPF 313 FVVR+ LDD + A LPAGS G AAI+T+H K THV+RRV+LR AI+NYVNP+ Sbjct: 260 FVVRIALDDPSLAASLPAGSTGDAAIFTDHAKATHVIRRVMLRMSAIMNYVNPY 313 >ref|YP_001768169.1| secretion protein HlyD family protein [Methylobacterium sp. 4-46] gb|ACA15735.1| secretion protein HlyD family protein [Methylobacterium sp. 4-46] Length = 311 Score = 405 bits (1040), Expect = e-111, Method: Compositional matrix adjust. Identities = 205/313 (65%), Positives = 246/313 (78%), Gaps = 2/313 (0%) Query: 1 MGVAIINTYLVLLFLLVHFKIVRFNLFWKLSPFIVLILVMFGLLVPMNWGAPQGSALVVR 60 M V ++N+Y+ LL L V + + FNLFWKLSP +VLIL++ GL +PM WGAP G A V+R Sbjct: 1 MIVFLLNSYIALLVLFVWLRFIPFNLFWKLSPALVLILLLVGLFIPMGWGAPSGPAAVIR 60 Query: 61 NAVSIVPSVAGEVTEVPVVANAPLKAGDVLFKIDPTPYDAQVKAITAQLKLSKTRLTQMT 120 N+V IVPSVAGEV +VPV AN PLK GDVLF+IDPTPY QV+AI AQLKL++TRL +M+ Sbjct: 61 NSVQIVPSVAGEVIDVPVKANTPLKEGDVLFRIDPTPYQTQVEAIGAQLKLAETRLAEMS 120 Query: 121 TLFERDAGRGFDVEQRQSEVDQLSGQLEAAQWNLDKTVVTAPADGYVTNVALRKGARVTN 180 L R GR FDVEQRQ+E DQL QLE A+WNLDKT V APA GYVTN+ LRKGARVT Sbjct: 121 QLQSRGTGRAFDVEQRQAEADQLRAQLEGAKWNLDKTTVRAPAAGYVTNLTLRKGARVTA 180 Query: 181 LPLSPVMAFIDTSNTIIGVQINQIDARYVAPGQEVEVTFKFAPGQIYSGKVESVLQAIAT 240 SPVMAFIDT +T++G +I QI ARY+ PGQ VE+TFK PG ++SG+VE+VLQAIAT Sbjct: 181 --QSPVMAFIDTGDTLLGAEIPQIYARYIEPGQPVEITFKTLPGTVHSGRVEAVLQAIAT 238 Query: 241 GQTQTSGTAVAPKAIEAAPFVVRVKLDDATFANRLPAGSAGTAAIYTEHLKPTHVVRRVL 300 GQ Q G+AVAP I++APFVVR LDDA A RLPAGS GTAAIYT+H++ HV+R+VL Sbjct: 239 GQAQFGGSAVAPTDIQSAPFVVRFTLDDAEVARRLPAGSTGTAAIYTDHVQQAHVIRKVL 298 Query: 301 LRQVAIINYVNPF 313 LRQ AI+NY NPF Sbjct: 299 LRQSAILNYANPF 311