BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0034.p (336 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAR97436.1| nitrilase [uncultured organism] 540 e-151 ref|YP_001888465.1| nitrilase/cyanide hydratase and apolipoprote... 538 e-151 ref|YP_001862268.1| Nitrilase/cyanide hydratase and apolipoprote... 537 e-150 gb|AAR97418.1| nitrilase [uncultured organism] 527 e-147 gb|AAR97407.1| nitrilase [uncultured organism] 519 e-145 >gb|AAR97436.1| nitrilase [uncultured organism] Length = 330 Score = 540 bits (1391), Expect = e-151, Method: Compositional matrix adjust. Identities = 258/314 (82%), Positives = 282/314 (89%), Gaps = 2/314 (0%) Query: 1 MPVIKAAAVQISPVLYSREGTVGKVVKKIIELGRQGVQFATFPETVIPYYPYFSFVEAPY 60 M ++KAAAVQISPVLYSREGTV KVV+KI+ELG+QGVQFATFPETV+PYYPYFSFV++ Y Sbjct: 1 MTIVKAAAVQISPVLYSREGTVDKVVQKILELGKQGVQFATFPETVVPYYPYFSFVQSGY 60 Query: 61 LM--GAEHYKLLDQAVTVPSDVTRAIGEAAKQAGMVVSIGVNERDGGTIYNAQILFDADG 118 + G EH +LL+Q+VTVPS T AIGEA KQAGMVVSIGVNERDG TIYN Q+LFDADG Sbjct: 61 ALKVGKEHLRLLEQSVTVPSATTLAIGEACKQAGMVVSIGVNERDGSTIYNTQLLFDADG 120 Query: 119 MLIQHRRKISPTYHERMVWGQGDGSGLRCVDSAVGRIGQLACWEHYNPLARYALMADGEQ 178 LIQ RRKISPT+HERMVWGQGDGSGLR VDSAVGRIGQLACWEHYNPLARYA+MADGEQ Sbjct: 121 TLIQRRRKISPTFHERMVWGQGDGSGLRAVDSAVGRIGQLACWEHYNPLARYAMMADGEQ 180 Query: 179 IHSAMYPGSFAGDGFSEQISVNIRQHALEAGCFVVCATAWLDAEQQARIMTDTGSRIEPI 238 IHSAMYPGSFAGD FSEQI VNIRQHALEAGCFVV ATAWLDA+QQA+IM DTG I PI Sbjct: 181 IHSAMYPGSFAGDAFSEQIQVNIRQHALEAGCFVVNATAWLDADQQAQIMQDTGCAIGPI 240 Query: 239 SSGCFTAIVSPEGELMGAPLRSREGEVIADLDFWAIDKRKRLMDSRGHYSRPELLSLLID 298 SSGCFTAIVSP+G L+G PLRS EGEVIADLDF IDKRK++MDSRGHY+RPELLSLLID Sbjct: 241 SSGCFTAIVSPDGVLLGEPLRSGEGEVIADLDFTLIDKRKQMMDSRGHYARPELLSLLID 300 Query: 299 RTPKSHVLERNAHP 312 RT +HV ER+AHP Sbjct: 301 RTATAHVHERSAHP 314 >ref|YP_001888465.1| nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase [Burkholderia phytofirmans PsJN] gb|ACD19095.1| Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase [Burkholderia phytofirmans PsJN] Length = 328 Score = 538 bits (1386), Expect = e-151, Method: Compositional matrix adjust. Identities = 254/309 (82%), Positives = 283/309 (91%), Gaps = 2/309 (0%) Query: 3 VIKAAAVQISPVLYSREGTVGKVVKKIIELGRQGVQFATFPETVIPYYPYFSFVEAPYLM 62 +I+AAAVQI+PVLYSREGTV KVV+KI+ELG+QGVQFATFPETV+PYYPYFSF+E+P+ M Sbjct: 4 IIRAAAVQIAPVLYSREGTVDKVVRKILELGKQGVQFATFPETVVPYYPYFSFIESPFAM 63 Query: 63 GAEHYKLLDQAVTVPSDVTRAIGEAAKQAGMVVSIGVNERDGGTIYNAQILFDADGMLIQ 122 GAEHYKLLDQAVTVPS+ TRAIGEAA+QAGMVVSIGVNERDGGTIYN Q+LFDADG L+Q Sbjct: 64 GAEHYKLLDQAVTVPSETTRAIGEAARQAGMVVSIGVNERDGGTIYNTQLLFDADGTLVQ 123 Query: 123 HRRKISPTYHERMVWGQGDGSGLRCVDSAVGRIGQLACWEHYNPLARYALMADGEQIHSA 182 RRK+SPTYHERMVWG GDGSGLR VD+AVGR+GQLACWEHYNPLARYALMADGE+IHSA Sbjct: 124 RRRKLSPTYHERMVWGMGDGSGLRAVDTAVGRVGQLACWEHYNPLARYALMADGEEIHSA 183 Query: 183 MYPGSFAGDGFSEQISVNIRQHALEAGCFVVCATAWLDAEQQARIMTDTG-SRIEPISSG 241 MYPGSFAGD FSEQISVNIRQHALEAG FVV ATAWL EQQ +I+ DT S++ PISSG Sbjct: 184 MYPGSFAGDLFSEQISVNIRQHALEAGAFVVNATAWLTPEQQQQILHDTNTSQVGPISSG 243 Query: 242 CFTAIVSPEGELMGA-PLRSREGEVIADLDFWAIDKRKRLMDSRGHYSRPELLSLLIDRT 300 CFTAIVSP+GE MG PLR EGE+IADLDFW IDKRKR+MDSRGHYSRP++L L+IDRT Sbjct: 244 CFTAIVSPDGEYMGGQPLREGEGEIIADLDFWQIDKRKRMMDSRGHYSRPDVLGLVIDRT 303 Query: 301 PKSHVLERN 309 PK+HVLER Sbjct: 304 PKAHVLERT 312 >ref|YP_001862268.1| Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase [Burkholderia phymatum STM815] gb|ACC75222.1| Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase [Burkholderia phymatum STM815] Length = 328 Score = 537 bits (1383), Expect = e-150, Method: Compositional matrix adjust. Identities = 252/309 (81%), Positives = 284/309 (91%), Gaps = 2/309 (0%) Query: 3 VIKAAAVQISPVLYSREGTVGKVVKKIIELGRQGVQFATFPETVIPYYPYFSFVEAPYLM 62 +I+AAAVQI+PVLYSREGTV KVV+KI+ELGRQGVQFATFPET++PYYPYFSF+E+P+ M Sbjct: 4 IIRAAAVQIAPVLYSREGTVDKVVRKILELGRQGVQFATFPETIVPYYPYFSFIESPFAM 63 Query: 63 GAEHYKLLDQAVTVPSDVTRAIGEAAKQAGMVVSIGVNERDGGTIYNAQILFDADGMLIQ 122 GAEHYKLL+QAVTVPSD TRAIG+AA+QAGMVVSIGVNERDGGTIYN Q+LFDADG L+Q Sbjct: 64 GAEHYKLLEQAVTVPSDTTRAIGDAARQAGMVVSIGVNERDGGTIYNTQLLFDADGTLVQ 123 Query: 123 HRRKISPTYHERMVWGQGDGSGLRCVDSAVGRIGQLACWEHYNPLARYALMADGEQIHSA 182 RRK+SPTYHERMVWG GDGSGLR +D+AVGR+GQLACWEHYNPLARYALMADGE+IHSA Sbjct: 124 RRRKLSPTYHERMVWGMGDGSGLRAIDTAVGRVGQLACWEHYNPLARYALMADGEEIHSA 183 Query: 183 MYPGSFAGDGFSEQISVNIRQHALEAGCFVVCATAWLDAEQQARIMTDTG-SRIEPISSG 241 MYPGSFAGD FSEQISVNIRQHALEAG FVV ATAWL EQQ +I+ DT ++I PISSG Sbjct: 184 MYPGSFAGDLFSEQISVNIRQHALEAGAFVVNATAWLTPEQQQQILQDTNTTQIGPISSG 243 Query: 242 CFTAIVSPEGELM-GAPLRSREGEVIADLDFWAIDKRKRLMDSRGHYSRPELLSLLIDRT 300 CFTAI+SPEGE M GAPLR EGE+IADLDFW IDKRKR+MDSRGHYSRP++L L+IDRT Sbjct: 244 CFTAIISPEGEYMGGAPLREGEGEIIADLDFWQIDKRKRMMDSRGHYSRPDVLGLVIDRT 303 Query: 301 PKSHVLERN 309 PK HV+ER+ Sbjct: 304 PKLHVIERS 312 >gb|AAR97418.1| nitrilase [uncultured organism] Length = 325 Score = 527 bits (1357), Expect = e-147, Method: Compositional matrix adjust. Identities = 250/311 (80%), Positives = 275/311 (88%) Query: 1 MPVIKAAAVQISPVLYSREGTVGKVVKKIIELGRQGVQFATFPETVIPYYPYFSFVEAPY 60 MP+IKAAAVQISPVLYSREGTV KV ++II+LGRQGVQFA FPETV+PYYPYFSFV+ + Sbjct: 1 MPIIKAAAVQISPVLYSREGTVDKVCQQIIDLGRQGVQFAVFPETVVPYYPYFSFVQPAF 60 Query: 61 LMGAEHYKLLDQAVTVPSDVTRAIGEAAKQAGMVVSIGVNERDGGTIYNAQILFDADGML 120 MGA+H KLLDQ+VTVPS T AIGEA KQAG+VVSIGVNERDGGTIYNAQ+LFDADG L Sbjct: 61 AMGAQHLKLLDQSVTVPSAATLAIGEACKQAGIVVSIGVNERDGGTIYNAQLLFDADGSL 120 Query: 121 IQHRRKISPTYHERMVWGQGDGSGLRCVDSAVGRIGQLACWEHYNPLARYALMADGEQIH 180 IQHRRKI+PTYHERMVWGQGDGSGLR +DSAVGRIG LACWEHYNPLARYALMADGEQIH Sbjct: 121 IQHRRKITPTYHERMVWGQGDGSGLRAIDSAVGRIGSLACWEHYNPLARYALMADGEQIH 180 Query: 181 SAMYPGSFAGDGFSEQISVNIRQHALEAGCFVVCATAWLDAEQQARIMTDTGSRIEPISS 240 +AM+PGS GD F+EQI V IR HALE+GCFVV ATAWLDA+QQ +IM DTG + PIS Sbjct: 181 AAMFPGSLVGDIFAEQIEVTIRHHALESGCFVVNATAWLDADQQGQIMQDTGCSLGPISG 240 Query: 241 GCFTAIVSPEGELMGAPLRSREGEVIADLDFWAIDKRKRLMDSRGHYSRPELLSLLIDRT 300 GCFTAIVSPEG+L+G PLRS EG VIADLD IDKRKR+MDS GHYSRPELLSLLIDRT Sbjct: 241 GCFTAIVSPEGKLLGEPLRSGEGVVIADLDLALIDKRKRMMDSVGHYSRPELLSLLIDRT 300 Query: 301 PKSHVLERNAH 311 P +HV ER+AH Sbjct: 301 PTAHVHERSAH 311 >gb|AAR97407.1| nitrilase [uncultured organism] Length = 326 Score = 519 bits (1336), Expect = e-145, Method: Compositional matrix adjust. Identities = 246/311 (79%), Positives = 271/311 (87%) Query: 1 MPVIKAAAVQISPVLYSREGTVGKVVKKIIELGRQGVQFATFPETVIPYYPYFSFVEAPY 60 M +IKAAAVQISPVLYSREGTV KV ++II LG+QGVQFA FPETV+PYYPYFSFV+ P+ Sbjct: 1 MAIIKAAAVQISPVLYSREGTVDKVCQQIIALGQQGVQFAVFPETVVPYYPYFSFVQPPF 60 Query: 61 LMGAEHYKLLDQAVTVPSDVTRAIGEAAKQAGMVVSIGVNERDGGTIYNAQILFDADGML 120 MG EH KLL+Q+V VPS T AIGEA KQAGMVVSIGVNERDGGTIYNAQ+LFDADG L Sbjct: 61 AMGKEHLKLLEQSVIVPSAATLAIGEACKQAGMVVSIGVNERDGGTIYNAQLLFDADGSL 120 Query: 121 IQHRRKISPTYHERMVWGQGDGSGLRCVDSAVGRIGQLACWEHYNPLARYALMADGEQIH 180 IQHRRKI+PTYHERM+WGQGDGSGLR +DSAVGRIG LACWEHYNPLARYALMADGEQIH Sbjct: 121 IQHRRKITPTYHERMIWGQGDGSGLRAIDSAVGRIGSLACWEHYNPLARYALMADGEQIH 180 Query: 181 SAMYPGSFAGDGFSEQISVNIRQHALEAGCFVVCATAWLDAEQQARIMTDTGSRIEPISS 240 +AM+PGS GD F++QI V IR HALE+GCFVV +TAWLDA+QQ +IM DTG I PIS Sbjct: 181 AAMFPGSLVGDIFADQIEVTIRHHALESGCFVVNSTAWLDADQQGQIMQDTGCSIGPISG 240 Query: 241 GCFTAIVSPEGELMGAPLRSREGEVIADLDFWAIDKRKRLMDSRGHYSRPELLSLLIDRT 300 GCFTAIVSPEG+L+G PLRS EG VIADLD IDKRKR+MDS GHYSRPELLSLLIDRT Sbjct: 241 GCFTAIVSPEGKLLGEPLRSGEGAVIADLDMALIDKRKRMMDSVGHYSRPELLSLLIDRT 300 Query: 301 PKSHVLERNAH 311 P +HV ER AH Sbjct: 301 PTAHVHERGAH 311