BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0006.p (355 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_07028559.1| efflux transporter, RND family, MFP subunit [... 568 e-160 ref|NP_770751.1| hypothetical protein blr411 [Bradyrhizobium jap... 524 e-147 ref|YP_001242067.1| putative cation efflux membrane fusion prote... 516 e-144 ref|YP_001207613.1| putative cation efflux membrane fusion prote... 504 e-141 ref|YP_002290418.1| secretion protein HlyD [Oligotropha carboxid... 431 e-118 >ref|ZP_07028559.1| efflux transporter, RND family, MFP subunit [Afipia sp. 1NLS2] gb|EFI49982.1| efflux transporter, RND family, MFP subunit [Afipia sp. 1NLS2] Length = 374 Score = 568 bits (1463), Expect = e-160, Method: Compositional matrix adjust. Identities = 283/355 (79%), Positives = 317/355 (89%) Query: 1 LALIGLPSFAMAEDAIKLTPEQATSLGVRVVHPVASPTDKTLPYPAQIVIPTPQLWVVSS 60 L + LP+ ++AED +KL+PEQATSLG+RVVHPVASPTDKTLPYPAQIVIPTPQLWVVS+ Sbjct: 20 LTFLALPAKSVAEDQLKLSPEQATSLGIRVVHPVASPTDKTLPYPAQIVIPTPQLWVVSA 79 Query: 61 PVAGMVTGLSVARGDRVSAGQPLVVMESPSFVSQQRDYMQAVAQDILATQQLNRNTQLFQ 120 PVAGMVT LSVARGDRV AGQPL MESP FVSQQRDY+ AVAQ+ LA QQLNRNTQLFQ Sbjct: 80 PVAGMVTNLSVARGDRVVAGQPLATMESPGFVSQQRDYLHAVAQEGLAAQQLNRNTQLFQ 139 Query: 121 TNAVAQRVLEASQTEARQASILLAERRQMLRLSGLSDDAISKLTSEAAINATLSVFAPRA 180 NAVAQRVLEASQ EARQA I +AERRQ+LRLSGLSD+AISKL++EAAI+ATL+V PR Sbjct: 140 GNAVAQRVLEASQAEARQAGIAVAERRQVLRLSGLSDEAISKLSNEAAISATLTVATPRP 199 Query: 181 ATVVDIVVSPGQRVEQTASLIKLAQLSPLWIEIAVPASNIRAIRPGAKVEVEGYTVPGQV 240 ATV+DI VSPGQRVEQ+A LIK+AQLSPLW+EIAVPASNI+AIR GAKVEVEG+ PG+V Sbjct: 200 ATVIDIAVSPGQRVEQSAPLIKVAQLSPLWVEIAVPASNIKAIRQGAKVEVEGHETPGEV 259 Query: 241 VLISETADAATQTILVRAEVPNTGELRPGQTAQARIGFASSTESAWELPYSALIRRGEQA 300 VL+SET D ATQT+L+RAEVPN+G+LRPGQTA ARI FAS ESAWE+PYSALIRRGEQA Sbjct: 260 VLVSETTDPATQTVLIRAEVPNSGDLRPGQTAAARISFASKRESAWEVPYSALIRRGEQA 319 Query: 301 TVFVAIPGGFRLVAVKLLEEDQDHVVVSGPITDKDEVAAGGISALRGILLKLGAG 355 +VFV+ GGFRL+ V LLEEDQDHVVVSG +TDKDEVA GISALRGILLKLGAG Sbjct: 320 SVFVSTQGGFRLMPVTLLEEDQDHVVVSGALTDKDEVAVSGISALRGILLKLGAG 374 >ref|NP_770751.1| hypothetical protein blr411 [Bradyrhizobium japonicum USDA 110] dbj|BAC49376.1| blr4111 [Bradyrhizobium japonicum USDA 110] Length = 376 Score = 524 bits (1350), Expect = e-147, Method: Compositional matrix adjust. Identities = 258/355 (72%), Positives = 302/355 (85%) Query: 1 LALIGLPSFAMAEDAIKLTPEQATSLGVRVVHPVASPTDKTLPYPAQIVIPTPQLWVVSS 60 L+L+G P A A+D +KLTP Q SLG+RVVHPV+S TD+TLPYPAQIV+PTPQLWVVS+ Sbjct: 21 LSLVGCPGIAAADDDMKLTPTQIESLGIRVVHPVSSRTDQTLPYPAQIVVPTPQLWVVST 80 Query: 61 PVAGMVTGLSVARGDRVSAGQPLVVMESPSFVSQQRDYMQAVAQDILATQQLNRNTQLFQ 120 PVAGMVT L+VARGDRVSAGQPL+ +ES FVS QRDY+ AVAQ+ LA QQL RN LF+ Sbjct: 81 PVAGMVTNLAVARGDRVSAGQPLLTLESQGFVSLQRDYLHAVAQEALANQQLRRNADLFE 140 Query: 121 TNAVAQRVLEASQTEARQASILLAERRQMLRLSGLSDDAISKLTSEAAINATLSVFAPRA 180 AV QRVLEASQTEARQA+I +AERRQMLRL+GLSDDAIS+L S+AAI+ TL+V AP+ Sbjct: 141 GKAVPQRVLEASQTEARQATIAVAERRQMLRLAGLSDDAISRLASDAAISGTLTVNAPQT 200 Query: 181 ATVVDIVVSPGQRVEQTASLIKLAQLSPLWIEIAVPASNIRAIRPGAKVEVEGYTVPGQV 240 +VV+I VSPGQR+EQ+A L+KLA+LSPLW EIA+P +NIRAIR GA+VEVEGY PG+V Sbjct: 201 GSVVEIAVSPGQRLEQSAPLVKLARLSPLWAEIAIPGANIRAIRTGARVEVEGYVTPGRV 260 Query: 241 VLISETADAATQTILVRAEVPNTGELRPGQTAQARIGFASSTESAWELPYSALIRRGEQA 300 VLISET DAATQTILVRAEVPN+G+L PGQTA R+ F S ESAWE+PYS L+RRGEQ+ Sbjct: 261 VLISETTDAATQTILVRAEVPNSGQLHPGQTASVRLSFLSEGESAWEIPYSGLVRRGEQS 320 Query: 301 TVFVAIPGGFRLVAVKLLEEDQDHVVVSGPITDKDEVAAGGISALRGILLKLGAG 355 +F AI GGFRLV+V LL EDQDHVV+SGPI+DKDEVA GISALRGIL +LGAG Sbjct: 321 WIFKAIEGGFRLVSVTLLAEDQDHVVISGPISDKDEVAIAGISALRGILSRLGAG 375 >ref|YP_001242067.1| putative cation efflux membrane fusion protein [Bradyrhizobium sp. BTAi1] gb|ABQ38161.1| putative cation efflux membrane fusion protein [Bradyrhizobium sp. BTAi1] Length = 375 Score = 516 bits (1330), Expect = e-144, Method: Compositional matrix adjust. Identities = 256/353 (72%), Positives = 306/353 (86%), Gaps = 1/353 (0%) Query: 1 LALIGLPSFAMAEDAIKLTPEQATSLGVRVVHPVASPTDKTLPYPAQIVIPTPQLWVVSS 60 LAL P+ A+AED +KLT EQA +LG+RV HPV SPTDKTLPYPAQIVIPTPQLWVVS+ Sbjct: 23 LALGTSPARAVAED-VKLTAEQARNLGIRVTHPVPSPTDKTLPYPAQIVIPTPQLWVVSA 81 Query: 61 PVAGMVTGLSVARGDRVSAGQPLVVMESPSFVSQQRDYMQAVAQDILATQQLNRNTQLFQ 120 PVAGMVT L+V RGDRV+ GQPL+ +ESPSFVS QR+Y+ A+AQ++LA QQL RN LF Sbjct: 82 PVAGMVTNLAVGRGDRVTTGQPLLTLESPSFVSLQREYLHALAQEVLAAQQLKRNADLFD 141 Query: 121 TNAVAQRVLEASQTEARQASILLAERRQMLRLSGLSDDAISKLTSEAAINATLSVFAPRA 180 AV QRVLE+SQ EARQASI++AERRQML LSGLSDDAI++LT+EAAI+ATL+V AP+A Sbjct: 142 GKAVPQRVLESSQAEARQASIVVAERRQMLHLSGLSDDAIARLTNEAAISATLTVNAPQA 201 Query: 181 ATVVDIVVSPGQRVEQTASLIKLAQLSPLWIEIAVPASNIRAIRPGAKVEVEGYTVPGQV 240 A+VV+I VSPGQR+EQ+A L+KLA+LSPLW+EIA+PA+NI AI+ GAKVE++GY PG+V Sbjct: 202 ASVVEIAVSPGQRMEQSAPLVKLARLSPLWVEIAIPATNIGAIKIGAKVEIDGYPTPGRV 261 Query: 241 VLISETADAATQTILVRAEVPNTGELRPGQTAQARIGFASSTESAWELPYSALIRRGEQA 300 +L+SET DAATQTILVRAE+PN GEL GQTA ARIGF S+ ESAWE+PYS L+RRGEQ Sbjct: 262 ILVSETTDAATQTILVRAEIPNNGELHSGQTAAARIGFISAGESAWEIPYSGLVRRGEQT 321 Query: 301 TVFVAIPGGFRLVAVKLLEEDQDHVVVSGPITDKDEVAAGGISALRGILLKLG 353 ++FVAI GGFR V V LL EDQDHVVVSGPIT++++VA GGISALRGILL+LG Sbjct: 322 SIFVAIEGGFRQVPVTLLAEDQDHVVVSGPITEQNDVAVGGISALRGILLRLG 374 >ref|YP_001207613.1| putative cation efflux membrane fusion protein [Bradyrhizobium sp. ORS 278] emb|CAL79396.1| putative cation efflux membrane fusion protein (CusB-like) [Bradyrhizobium sp. ORS 278] Length = 374 Score = 504 bits (1299), Expect = e-141, Method: Compositional matrix adjust. Identities = 246/347 (70%), Positives = 296/347 (85%), Gaps = 1/347 (0%) Query: 7 PSFAMAEDAIKLTPEQATSLGVRVVHPVASPTDKTLPYPAQIVIPTPQLWVVSSPVAGMV 66 P A AED +KLTP Q +LG+RV HP +S D+TLPYPAQIVIPTPQLWVVS+PV+GMV Sbjct: 28 PCAASAED-VKLTPAQTQNLGIRVAHPTSSRNDQTLPYPAQIVIPTPQLWVVSAPVSGMV 86 Query: 67 TGLSVARGDRVSAGQPLVVMESPSFVSQQRDYMQAVAQDILATQQLNRNTQLFQTNAVAQ 126 T L+VARGDRVS GQPL+ +ESPSFVS QR+Y+ A+AQ++LA QQL RN LF+ A+ Q Sbjct: 87 TNLAVARGDRVSIGQPLLTLESPSFVSLQREYLHALAQEVLAVQQLKRNADLFEGKAIPQ 146 Query: 127 RVLEASQTEARQASILLAERRQMLRLSGLSDDAISKLTSEAAINATLSVFAPRAATVVDI 186 RVLE+SQ EARQASI++AERRQML LSGLSD+A+++LT+EAAI+ATL+V AP+AA+VVDI Sbjct: 147 RVLESSQAEARQASIVVAERRQMLHLSGLSDEAVARLTNEAAISATLTVNAPQAASVVDI 206 Query: 187 VVSPGQRVEQTASLIKLAQLSPLWIEIAVPASNIRAIRPGAKVEVEGYTVPGQVVLISET 246 +VSPGQR+EQ A L+KLA+LSPLW EIA+PA+NI AI+ GA+V++EGY PG+V+L+SET Sbjct: 207 MVSPGQRMEQAAPLVKLARLSPLWAEIAIPATNIAAIKTGARVDIEGYATPGRVILVSET 266 Query: 247 ADAATQTILVRAEVPNTGELRPGQTAQARIGFASSTESAWELPYSALIRRGEQATVFVAI 306 D ATQTILVRAE+PN G L GQTA ARIGF S+ ESAWE+PYS L+RRGEQ ++FVAI Sbjct: 267 TDTATQTILVRAEIPNDGGLHSGQTAAARIGFVSAGESAWEIPYSGLVRRGEQTSIFVAI 326 Query: 307 PGGFRLVAVKLLEEDQDHVVVSGPITDKDEVAAGGISALRGILLKLG 353 GGFR V V LL EDQDHVVVSGPI+DKDEVA GGISALRGIL +LG Sbjct: 327 EGGFRQVPVTLLAEDQDHVVVSGPISDKDEVAVGGISALRGILSRLG 373 >ref|YP_002290418.1| secretion protein HlyD [Oligotropha carboxidovorans OM5] ref|YP_004631646.1| membrane fusion protein [Oligotropha carboxidovorans OM5] gb|ACI94553.1| secretion protein HlyD [Oligotropha carboxidovorans OM5] gb|AEI01830.1| membrane fusion protein [Oligotropha carboxidovorans OM4] gb|AEI05405.1| membrane fusion protein [Oligotropha carboxidovorans OM5] Length = 373 Score = 431 bits (1108), Expect = e-118, Method: Compositional matrix adjust. Identities = 209/343 (60%), Positives = 268/343 (78%) Query: 13 EDAIKLTPEQATSLGVRVVHPVASPTDKTLPYPAQIVIPTPQLWVVSSPVAGMVTGLSVA 72 E + L PEQ SLG+R HPV SPTD+T+P+ A IVIPTP LWVVS+P+AGMVT L+ A Sbjct: 30 EQELMLNPEQIHSLGIRTAHPVPSPTDQTVPFSAAIVIPTPHLWVVSTPIAGMVTSLAAA 89 Query: 73 RGDRVSAGQPLVVMESPSFVSQQRDYMQAVAQDILATQQLNRNTQLFQTNAVAQRVLEAS 132 RG++V GQPLV MESP+FVS QRDY+ A+AQD L QL RN +L AV QRV+EAS Sbjct: 90 RGEQVLRGQPLVTMESPAFVSLQRDYLHALAQDALQNLQLRRNRELLAGRAVPQRVVEAS 149 Query: 133 QTEARQASILLAERRQMLRLSGLSDDAISKLTSEAAINATLSVFAPRAATVVDIVVSPGQ 192 +TEARQA I L ERRQMLRLSG+S+ A+++LT EAAI+ +L+V AP + T+VD +SPG Sbjct: 150 ETEARQADIALTERRQMLRLSGMSEAALARLTDEAAISPSLTVVAPESGTIVDFTISPGV 209 Query: 193 RVEQTASLIKLAQLSPLWIEIAVPASNIRAIRPGAKVEVEGYTVPGQVVLISETADAATQ 252 R+EQ++ L+K+A+LSPLW+EIAVPA+ + A++ GA+V+VEGY PG+VVL+S T D ATQ Sbjct: 210 RLEQSSPLLKIARLSPLWVEIAVPATTVGAVKVGARVDVEGYDTPGKVVLVSATTDPATQ 269 Query: 253 TILVRAEVPNTGELRPGQTAQARIGFASSTESAWELPYSALIRRGEQATVFVAIPGGFRL 312 T+LVRAE+ N G LRPGQ A ARIG+AS ESAW++P A+ RRGE A+VFVA GFR+ Sbjct: 270 TVLVRAEIANDGTLRPGQAASARIGYASQAESAWQIPAGAMARRGETASVFVATEAGFRI 329 Query: 313 VAVKLLEEDQDHVVVSGPITDKDEVAAGGISALRGILLKLGAG 355 V VK++ EDQDH+ V G +T +D +A G+SALRG+LL LG+G Sbjct: 330 VPVKVIAEDQDHLHVFGALTSEDALAVSGLSALRGMLLGLGSG 372