BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0001.p (407 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_298987.1| phthalate 4,5-dioxygenase [Ralstonia eutropha J... 361 1e-97 ref|ZP_03266801.1| Phthalate 4,5-dioxygenase [Burkholderia sp. H... 355 1e-95 ref|ZP_03265564.1| Rieske (2Fe-2S) domain protein [Burkholderia ... 354 1e-95 ref|ZP_03265572.1| Phthalate 4,5-dioxygenase [Burkholderia sp. H... 343 4e-92 ref|YP_001535493.1| phthalate 4,5-dioxygenase [Salinispora areni... 290 4e-76 >ref|YP_298987.1| phthalate 4,5-dioxygenase [Ralstonia eutropha JMP134] gb|AAZ64143.1| Phthalate 4,5-dioxygenase [Ralstonia eutropha JMP134] Length = 412 Score = 361 bits (926), Expect = 1e-97, Method: Compositional matrix adjust. Identities = 188/413 (45%), Positives = 250/413 (60%), Gaps = 12/413 (2%) Query: 2 VTREENELLCRVTGDAPMGRMLRQHYWLPAVLSVRVRKGEAPVRVKLFGRNFVAFRGKDG 61 +TRE+NELL RV APMG M+RQHYWLPAV S ++ APVRV+L G N+VAFR DG Sbjct: 3 MTREDNELLTRVENGAPMGEMIRQHYWLPAVPSAKLVADGAPVRVRLLGSNYVAFRRTDG 62 Query: 62 SVGFLDEACPHRGASLALARVDDCSLTCLFHGWKINAQGEVLDVPNEPNNPKEFAKTVKA 121 +VG LDE CPHR SL +AR +D L C+FHGWK+ GEV++ PN + +F K V+ Sbjct: 63 AVGVLDELCPHRKTSLLMARNEDNGLRCIFHGWKLGTTGEVMEAPNHVGDQAQFCKHVRV 122 Query: 122 KAYPVREGAGLIWVWLGEG-AAPPAPDFEWMDLPPDQVYAAGIELNANWLQGVEATIDSS 180 Y V+E G++WVWLG+G AP PD + LP +Q E+ NW+QGVEA++DSS Sbjct: 123 NRYGVQERGGIVWVWLGKGEQAPKFPDLPFTSLPEEQRSVTSQEVPTNWVQGVEASMDSS 182 Query: 181 HVALLHQSWTATSSSDFGATRVHTKVA----YEFDKKSYGFRAAALRTAPDGSCLARVTE 236 HV +LH+S T ++ G R+H A EF+ + YG+R AALR D ARV Sbjct: 183 HVGVLHESTTQITAGS-GNQRMHMTQALAPRLEFEDRPYGYRYAALRPLADDKLYARVNN 241 Query: 237 FVMPYYGLI-APIFSGPQQDRTAIIAVPIDDENLIQWYIFYNPEKPVDSWRKVQRANQWP 295 FVMP+YG+I AP GP T + P+DD W++ +NP +P+ + W Sbjct: 242 FVMPWYGIICAPDEKGPS---TVFFSTPVDDVTHRAWFVHFNPHRPLGMTIMSATPDVWN 298 Query: 296 MAGGLPGDQSNNWGQNRQIMDAGNSTGF-HEIVIEDFVVMVSMGPIVDRSLEYLCSADQA 354 PG ++NWGQNR +M G++TGF + EDF + + GPI DR+ E LCSAD A Sbjct: 299 FPPLPPGTPADNWGQNRDLMRRGHATGFPQHLGTEDFAMFIGQGPIADRTTEQLCSADGA 358 Query: 355 IVRVRRQLLDEVRGFMNGKAPKSGQSESMSYKDIRAVGGRLASAAEDWRQIPR 407 ++RVR+ LL VR F GK P + + Y I +VGG L A EDWR + + Sbjct: 359 VLRVRQALLKSVREFQAGKTPTLADNPDLDYSQIFSVGGVL-PAGEDWRALTK 410 >ref|ZP_03266801.1| Phthalate 4,5-dioxygenase [Burkholderia sp. H160] gb|EEA01599.1| Phthalate 4,5-dioxygenase [Burkholderia sp. H160] Length = 412 Score = 355 bits (910), Expect = 1e-95, Method: Compositional matrix adjust. Identities = 182/410 (44%), Positives = 248/410 (60%), Gaps = 10/410 (2%) Query: 2 VTREENELLCRVTGDAPMGRMLRQHYWLPAVLSVRVRKGEAPVRVKLFGRNFVAFRGKDG 61 +TRE+NELL RV APMG M+RQHYW+PAV S ++ APV V+L G N+VAFR DG Sbjct: 3 MTREDNELLTRVENGAPMGDMIRQHYWIPAVPSAKLEADGAPVLVRLLGTNYVAFRATDG 62 Query: 62 SVGFLDEACPHRGASLALARVDDCSLTCLFHGWKINAQGEVLDVPNEPNNPKEFAKTVKA 121 VG +DE CPHR SL +AR +D L C+FHGWKI+A G V++ PN+ + +F K VK Sbjct: 63 HVGVVDELCPHRRTSLMMARNEDNGLRCIFHGWKIDATGAVVEAPNQVGDQAQFCKRVKT 122 Query: 122 KAYPVREGAGLIWVWLGEGAAPPA-PDFEWMDLPPDQVYAAGIELNANWLQGVEATIDSS 180 Y V E GL+WVWLG+G PA P+ +MDLPP+ +E+ NW+Q VEA++DSS Sbjct: 123 GQYRVEERGGLVWVWLGKGDTSPAFPELPFMDLPPEHRSVTSVEVPTNWVQAVEASMDSS 182 Query: 181 HVALLHQSWTATSSSDFGATRVHTKVA---YEFDKKSYGFRAAALRTAPDGSCLARVTEF 237 HV +LH+S T ++ + TK +EF+ + YG+R AALR PD ARV F Sbjct: 183 HVGVLHESTTQITAGGGNERMIMTKALAPRFEFEDRPYGYRYAALRDMPDEKVYARVNNF 242 Query: 238 VMPYYGLIAPI-FSGPQQDRTAIIAVPIDDENLIQWYIFYNPEKPVDSWRKVQRANQWPM 296 VMP+YG+I P GP T + P+DD W++ YNP +P+ + W Sbjct: 243 VMPWYGIICPPDAKGPT---TVFFSTPVDDVTHRAWFVQYNPHRPLGMTIMSATPDVWNF 299 Query: 297 AGGLPGDQSNNWGQNRQIMDAGNSTGFHE-IVIEDFVVMVSMGPIVDRSLEYLCSADQAI 355 PG + ++GQ+R +M G++TGF++ + EDF + + GPI DR+ E LCS+D A+ Sbjct: 300 PPLPPGTREESFGQSRDLMKRGHATGFYQHLGTEDFAMFLGQGPIYDRTQEQLCSSDGAV 359 Query: 356 VRVRRQLLDEVRGFMNGKAPKSGQSESMSYKDIRAVGGRLASAAEDWRQI 405 +RVR LL R FM GK P S + Y +VGG L + A DWR + Sbjct: 360 LRVRALLLKAAREFMAGKTPTLADSPELDYSKAVSVGGVLQAGA-DWRSL 408 >ref|ZP_03265564.1| Rieske (2Fe-2S) domain protein [Burkholderia sp. H160] gb|EEA02833.1| Rieske (2Fe-2S) domain protein [Burkholderia sp. H160] Length = 402 Score = 354 bits (909), Expect = 1e-95, Method: Compositional matrix adjust. Identities = 176/396 (44%), Positives = 249/396 (62%), Gaps = 10/396 (2%) Query: 2 VTREENELLCRVTGDAPMGRMLRQHYWLPAVLSVRVRKGEAPVRVKLFGRNFVAFRGKDG 61 +T++ENELL RV+GDAPMG+M+RQ++W+PA+ S + G P RV+LFGRNFVAFRG +G Sbjct: 1 MTKDENELLTRVSGDAPMGQMIRQNWWIPALRSASLVAGGRPERVRLFGRNFVAFRGDNG 60 Query: 62 SVGFLDEACPHRGASLALARVDDCSLTCLFHGWKINAQGEVLDVPNEPNNPKEFAKTVKA 121 VGF DEACPHRGASLALAR +D +L C+FHGWK N++G VP +PNN E+ K V Sbjct: 61 KVGFFDEACPHRGASLALARNEDNALRCIFHGWKFNSEGVTFTVPTQPNNEAEYCKHVPL 120 Query: 122 KAYPVREGAGLIWVWLGEGAAPPAPDFEWMDLPPDQVYAAGIELNANWLQGVEATIDSSH 181 K YP +E G+ WV+LG+ P D ++ LP DQV + NWLQ VE T+DS+H Sbjct: 121 KHYPTQEAGGIAWVYLGQSEPPKIADLPFVGLPADQVVVHRQRIECNWLQAVETTMDSAH 180 Query: 182 VALLHQSWTATSSSDFGATRVHTKVAYEFDKKSYGFRAAALRTAPDGSCLARVTEFVMPY 241 V +LHQSW ++ V+ Y+ +K YGFR AA+R DG R FVMP+ Sbjct: 181 VGVLHQSWLEHEMTN---ASVNQAPVYKIKQKEYGFRYAAVRALEDGRSYVRTNSFVMPW 237 Query: 242 YGLIAPIFSGPQQDRTAIIAVPIDDENLIQWYIFYNPEKPVDSWRKVQRANQ--WPMAGG 299 YG+I+P +G +VPIDDEN W++ + +KPV + A++ WP Sbjct: 238 YGMISPRKAGDTTRSQMFFSVPIDDENTWYWHMTFILDKPVPHEDYIFPADRDNWP---P 294 Query: 300 LPGDQSNN-WGQNRQIMDAGNSTGFHEIV-IEDFVVMVSMGPIVDRSLEYLCSADQAIVR 357 +P ++ WGQ+R+ M G+ +GF + + EDF +++SMG + DR+ EYL + D A+++ Sbjct: 295 IPSRSDDDLWGQDREAMKNGHFSGFPQTLGTEDFAILLSMGTLQDRTKEYLGAGDGAVMQ 354 Query: 358 VRRQLLDEVRGFMNGKAPKSGQSESMSYKDIRAVGG 393 VRR L++ + F G PK Q E + Y+ + +GG Sbjct: 355 VRRCLMNSLTEFSAGSVPKLAQHEKIDYRSVMPLGG 390 >ref|ZP_03265572.1| Phthalate 4,5-dioxygenase [Burkholderia sp. H160] gb|EEA02841.1| Phthalate 4,5-dioxygenase [Burkholderia sp. H160] Length = 405 Score = 343 bits (879), Expect = 4e-92, Method: Compositional matrix adjust. Identities = 178/415 (42%), Positives = 248/415 (59%), Gaps = 21/415 (5%) Query: 1 MVTREENELLCRVTGDAPMGRMLRQHYWLPAVLSVRVRKGEAPVRVKLFGRNFVAFRGKD 60 M+++ +N+LL RV GDAPMG+MLRQH+W+P LS ++ P R++LFG N+VAFR Sbjct: 1 MLSKADNDLLTRVCGDAPMGKMLRQHWWMPVALSDKLEADGKPARIRLFGENYVAFRDTH 60 Query: 61 GSVGFLDEACPHRGASLALARVDDCSLTCLFHGWKINAQGEVLDVPNEPNNPKEFAKTVK 120 G +GF DEACPHRGASLAL R + L C+FHGWK NA+G + VP +PNN E+ K V Sbjct: 61 GKIGFFDEACPHRGASLALGRNEKNGLRCIFHGWKFNAEGVTVAVPTQPNNEAEYCKHVP 120 Query: 121 AKAYPVREGAGLIWVWLGEGAAPPA-PDFEWMDLPPDQVYAAGIELNANWLQGVEATIDS 179 K YPVRE G++W WLG+G PA P+ + LP + ++NANWLQGVE T+DS Sbjct: 121 LKHYPVREEGGMVWAWLGQGDQAPAFPELPCVGLPRENFVVFRQKVNANWLQGVETTMDS 180 Query: 180 SHVALLHQSWTATSSSDFGATRVHTKVAYEFDKKSYGFRAAALRTAPDGSCLARVTEFVM 239 +H+ +LHQ W D + + Y +K++GFR AA+R + RV FVM Sbjct: 181 AHLGVLHQEWL-KGFGDIELSSANMAPVYHIVQKAFGFRYAAVRALKENRSYVRVNSFVM 239 Query: 240 PYYGLIAPIFSGPQQDRTAIIAVPIDDENLIQWYIFY--------NPEKPVDSWRKVQRA 291 P+YG+I+P S + + +VPIDDEN+ + + Y NP D A Sbjct: 240 PWYGVISPNNS-ETRGGSMFFSVPIDDENIWYYQLTYRTDGKLVLNPTLVFDD------A 292 Query: 292 NQWPMAGGLPGDQSNNWGQNRQIMDAGNSTGFHEIV-IEDFVVMVSMGPIVDRSLEYLCS 350 + WP PG +NWGQ+R+ M G+ +GF +++ EDF V++SMGPI DR+ EYL + Sbjct: 293 DSWPPLP--PGPAEDNWGQDREAMKNGHFSGFTQMLGTEDFAVVMSMGPISDRTKEYLGA 350 Query: 351 ADQAIVRVRRQLLDEVRGFMNGKAPKSGQSESMSYKDIRAVGGRLASAAEDWRQI 405 D A++ VRR L++ V+ FMNG PK + + YK G + A WR + Sbjct: 351 GDGAVLAVRRCLMNSVKEFMNGDTPKLARHAEIDYKSATPASGTFENEA-GWRAL 404 >ref|YP_001535493.1| phthalate 4,5-dioxygenase [Salinispora arenicola CNS-205] gb|ABV96502.1| Phthalate 4,5-dioxygenase [Salinispora arenicola CNS-205] Length = 406 Score = 290 bits (741), Expect = 4e-76, Method: Compositional matrix adjust. Identities = 174/420 (41%), Positives = 238/420 (56%), Gaps = 33/420 (7%) Query: 1 MVTREENELLCRVTGDAPMGRMLRQHYWLPAVLSVRVRK-GEAPVRVKLFGRNFVAFRGK 59 M T + N+ L RV PMGR+LR+ YW+P + S R+ + G AP+ V+L G FV FR Sbjct: 1 MTTSKMNDQLTRVGPGTPMGRLLRE-YWMPVMRSGRLTEPGGAPMAVELLGEKFVVFRAD 59 Query: 60 DGSVGFLDEACPHRGASLALARVDDCSLTCLFHGWKINAQGEVLDVPNEPNNPKEFAKTV 119 DG++G EACPHRGASL LAR +DC+L C++HGWK + G+VL+ P+EP FA V Sbjct: 60 DGTLGCFAEACPHRGASLTLARNEDCALRCIYHGWKFSVTGQVLETPSEPAELTRFASRV 119 Query: 120 KAKAYPVREGAGLIWVWLGEG--AAPPAPDFEWMDLPPDQVYAAGIELNANWLQGVEATI 177 K + +PV E G+IWVW+G +AP P F + LPP+QV+ + NWLQG+EA I Sbjct: 120 KLRHHPVIESGGVIWVWIGGTGRSAPAPPAFTFTKLPPEQVFGLVAVVECNWLQGLEADI 179 Query: 178 DSSHVALLHQSWT-ATSSSDFGATRVHTKVAYEFDKKSYGFRAAALRTAPDGSCLARVTE 236 DS+HV+LLH++ A + D R E D++ YG R ++RT G+ L RV Sbjct: 180 DSAHVSLLHETEARAGALRDLLDDRTPRD---EIDEQPYGLRFGSVRTLSSGAELVRVKP 236 Query: 237 FVMPYYGLIAPIFSGPQQDRTAIIAVPIDDENLIQWYIFYNPEKPVDSWRKVQRANQWPM 296 F MP+Y ++ + SG DR VPI+D I WY++YN E+PVD A+Q+ Sbjct: 237 FAMPWYTVVPELPSG---DRLWHAWVPINDHRTIMWYLWYNEERPVD---PAVFADQF-- 288 Query: 297 AGGLPGD------------QSNNWGQNRQIMDAGNS-TGFHEIVIEDFVVMVSMGPIVDR 343 GL D + NNWGQ+R+ M S +G +V++D V SMGPIVDR Sbjct: 289 --GLNLDTMNPDNIREGFTRENNWGQDRRQMRENQSFSGIRGLVLQDIAVQESMGPIVDR 346 Query: 344 SLEYLCSADQAIVRVRRQLLDEVRGFMNGKAPKSGQSESMSYKDIRAVGGRLASAAEDWR 403 + E +D AIV RR LLD ++ G+ P G Y +R+ LA DWR Sbjct: 347 TGENPGRSDTAIVATRRYLLDAIKRHERGETPP-GLGPEADYDRVRSAEITLAPGV-DWR 404