BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0001.p (277 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_771572.1| cation efflux system protein [Bradyrhizobium ja... 450 e-125 ref|YP_001220420.1| secretion protein HlyD precursor [Bradyrhizo... 407 e-111 ref|YP_001239427.1| cation efflux system protein cusB [Bradyrhiz... 403 e-110 ref|YP_571781.1| secretion protein HlyD [Nitrobacter hamburgensi... 394 e-107 ref|YP_001239632.1| putative cation efflux system protein cusB [... 390 e-106 >ref|NP_771572.1| cation efflux system protein [Bradyrhizobium japonicum USDA 110] dbj|BAC50197.1| blr4932 [Bradyrhizobium japonicum USDA 110] Length = 478 Score = 450 bits (1158), Expect = e-125, Method: Compositional matrix adjust. Identities = 220/273 (80%), Positives = 242/273 (88%) Query: 1 VTKGQPLMEIYSPAISSAAAEYVATITSKATVGIEPYGRGSKQRLTNLDVPEPVIAEMEK 60 VTKGQ LMEIYSPA+SSAAAEY+ATITSK T GIEPYGRGS+QRL NLDVPEPVIAEMEK Sbjct: 194 VTKGQSLMEIYSPAVSSAAAEYLATITSKTTAGIEPYGRGSRQRLVNLDVPEPVIAEMEK 253 Query: 61 SRTVPIAIQWLSPRDGIVLQRAAIEGMRAQPGDVLFRIADVSVVWALVDVAERDLGNIVV 120 S VPI IQW SPRDGIVLQR AI+GMRAQPGD+LFRIAD+S+VWA VDVAERDLG I V Sbjct: 254 SHAVPITIQWSSPRDGIVLQRNAIDGMRAQPGDILFRIADISLVWANVDVAERDLGGIAV 313 Query: 121 GQKVTVRARSYPGRSFAGQISVIYPQVNKETRTARVRIELQNADLALLPDMYVDAEIDTG 180 GQ VTVRARSYPGR F G+ISVIYPQ+NKETRTAR+RIEL N DLALLPDMYVDA IDTG Sbjct: 314 GQPVTVRARSYPGRDFGGRISVIYPQLNKETRTARIRIELANDDLALLPDMYVDAAIDTG 373 Query: 181 GAAPVLTVADNSILDTGSRQSVLIDRGNGRFEPREVKLGRRGEGYVEIRDGLAEGDAVVT 240 APVL+VA++++LDTGSRQ+VL+D+G GRFEPREVKLGRRG+GYVEIRDGLAEGD +VT Sbjct: 374 NPAPVLSVAESAVLDTGSRQAVLVDKGQGRFEPREVKLGRRGDGYVEIRDGLAEGDVIVT 433 Query: 241 SATFLIDAESNLKAAIKGFAEAGAAQAGEGEKT 273 SA FLIDAESNLKAA+KG AE G AQ + T Sbjct: 434 SANFLIDAESNLKAALKGLAEGGNAQGTNAQGT 466 >ref|YP_001220420.1| secretion protein HlyD precursor [Bradyrhizobium sp. BTAi1] gb|ABQ39748.1| Secretion protein HlyD precursor [Bradyrhizobium sp. BTAi1] Length = 477 Score = 407 bits (1045), Expect = e-111, Method: Compositional matrix adjust. Identities = 204/278 (73%), Positives = 238/278 (85%), Gaps = 1/278 (0%) Query: 1 VTKGQPLMEIYSPAISSAAAEYVATITSKATVGIEPYGRGSKQRLTNLDVPEPVIAEMEK 60 V GQPLME+YS AI+SAAAEY+AT+ SK G+E YGRGS+QRLTNLDVPEPVI MEK Sbjct: 200 VKAGQPLMEVYSSAIASAAAEYLATLNSKVVGGVEAYGRGSRQRLTNLDVPEPVITAMEK 259 Query: 61 SRTVPIAIQWLSPRDGIVLQRAAIEGMRAQPGDVLFRIADVSVVWALVDVAERDLGNIVV 120 S+ P+ +QW +PRDGIVL+R AIEGMRA PGDVLFRIAD SVVWALVDVAERDLGNIVV Sbjct: 260 SKVAPVTVQWTAPRDGIVLERNAIEGMRANPGDVLFRIADTSVVWALVDVAERDLGNIVV 319 Query: 121 GQKVTVRARSYPGRSFAGQISVIYPQVNKETRTARVRIELQNADLALLPDMYVDAEIDTG 180 GQ VT+RARS+ GR+F+G+I+VIYPQVN++TRTARVRIEL N D ALLPDMYVDA+IDT Sbjct: 320 GQPVTIRARSFAGRTFSGKIAVIYPQVNRDTRTARVRIELSNPDAALLPDMYVDADIDTA 379 Query: 181 GAAPVLTVADNSILDTGSRQSVLIDRGNGRFEPREVKLGRRGEGYVEIRDGLAEGDAVVT 240 +PVL+V D+S+LDTG+RQ+VL+D+G GRFEPREVKLGRRG GYVEIRDGL +G++VVT Sbjct: 380 DGSPVLSVPDSSVLDTGARQAVLVDKGEGRFEPREVKLGRRGGGYVEIRDGLKDGESVVT 439 Query: 241 SATFLIDAESNLKAAIKGFAEAG-AAQAGEGEKTGDRR 277 SA FLIDAESNLKAA+KGFAEA A A G G+ + Sbjct: 440 SANFLIDAESNLKAALKGFAEANPAPDAASGHAHGEHK 477 >ref|YP_001239427.1| cation efflux system protein cusB [Bradyrhizobium sp. BTAi1] gb|ABQ35521.1| Cation efflux system protein cusB precursor [Bradyrhizobium sp. BTAi1] Length = 474 Score = 403 bits (1035), Expect = e-110, Method: Compositional matrix adjust. Identities = 200/274 (72%), Positives = 231/274 (84%) Query: 1 VTKGQPLMEIYSPAISSAAAEYVATITSKATVGIEPYGRGSKQRLTNLDVPEPVIAEMEK 60 V GQPLMEIYS AI++AAAEY++TI S YGRGS+QRLTNLDVPEPVI MEK Sbjct: 196 VKAGQPLMEIYSSAIAAAAAEYLSTINSNIVGSNAAYGRGSRQRLTNLDVPEPVITAMEK 255 Query: 61 SRTVPIAIQWLSPRDGIVLQRAAIEGMRAQPGDVLFRIADVSVVWALVDVAERDLGNIVV 120 SR P+ +QW +PRDGIVL+R A+EGMRA PGDVLFRIAD+S+VWALVDVAERDLG+I V Sbjct: 256 SRIAPVTVQWSAPRDGIVLERNAVEGMRANPGDVLFRIADISLVWALVDVAERDLGSIAV 315 Query: 121 GQKVTVRARSYPGRSFAGQISVIYPQVNKETRTARVRIELQNADLALLPDMYVDAEIDTG 180 GQ VT+RARS+PGR+F G I+VIYPQVNK+TRTARVRIEL N+DLALLPDMYVDAEIDT Sbjct: 316 GQPVTIRARSFPGRTFTGSIAVIYPQVNKDTRTARVRIELANSDLALLPDMYVDAEIDTA 375 Query: 181 GAAPVLTVADNSILDTGSRQSVLIDRGNGRFEPREVKLGRRGEGYVEIRDGLAEGDAVVT 240 APVL V +++LDTGSRQ+VLID+G+GR+EPR VKLGRRG GYVE+RDG+A+GDAVVT Sbjct: 376 APAPVLAVPTDAVLDTGSRQAVLIDKGDGRYEPRAVKLGRRGAGYVEVRDGVADGDAVVT 435 Query: 241 SATFLIDAESNLKAAIKGFAEAGAAQAGEGEKTG 274 SA FLIDAESNLKAA+K FAEAG Q + G Sbjct: 436 SANFLIDAESNLKAALKSFAEAGEPQPAGNDHAG 469 >ref|YP_571781.1| secretion protein HlyD [Nitrobacter hamburgensis X14] gb|ABE64949.1| Secretion protein HlyD [Nitrobacter hamburgensis X14] Length = 472 Score = 394 bits (1011), Expect = e-107, Method: Compositional matrix adjust. Identities = 190/264 (71%), Positives = 225/264 (85%) Query: 1 VTKGQPLMEIYSPAISSAAAEYVATITSKATVGIEPYGRGSKQRLTNLDVPEPVIAEMEK 60 V KGQPLMEIYSP++SSAAAEY+ TITSK T G YGRGS+QRL NLDVP+ I+ +EK Sbjct: 207 VVKGQPLMEIYSPSVSSAAAEYLTTITSKTTSGNVQYGRGSRQRLMNLDVPDAAISAIEK 266 Query: 61 SRTVPIAIQWLSPRDGIVLQRAAIEGMRAQPGDVLFRIADVSVVWALVDVAERDLGNIVV 120 SRTVP +I+W +PRDGIVL+R AIEGMR QPG VLFRIAD SVVWAL+DVAERDLG I V Sbjct: 267 SRTVPTSIEWSAPRDGIVLERNAIEGMRVQPGGVLFRIADHSVVWALIDVAERDLGAIAV 326 Query: 121 GQKVTVRARSYPGRSFAGQISVIYPQVNKETRTARVRIELQNADLALLPDMYVDAEIDTG 180 GQ VTV+ARS+PGR F G++ VIYP++NKETRTARVR+EL+NADL LL DMYVDAEI+TG Sbjct: 327 GQPVTVKARSFPGREFTGKVDVIYPEINKETRTARVRVELKNADLILLHDMYVDAEINTG 386 Query: 181 GAAPVLTVADNSILDTGSRQSVLIDRGNGRFEPREVKLGRRGEGYVEIRDGLAEGDAVVT 240 VL + ++++LDTG+RQSVLID+G GRFEPREVKL +RG GYVE+R G+A+G+ VV Sbjct: 387 SPEAVLAIPESAVLDTGARQSVLIDKGQGRFEPREVKLDQRGNGYVEVRSGVADGEPVVV 446 Query: 241 SATFLIDAESNLKAAIKGFAEAGA 264 SA FLIDAESNLKAA+KGF+E GA Sbjct: 447 SANFLIDAESNLKAALKGFSETGA 470 >ref|YP_001239632.1| putative cation efflux system protein cusB [Bradyrhizobium sp. BTAi1] gb|ABQ35726.1| putative Cation efflux system protein cusB precursor [Bradyrhizobium sp. BTAi1] Length = 475 Score = 390 bits (1001), Expect = e-106, Method: Compositional matrix adjust. Identities = 192/278 (69%), Positives = 236/278 (84%), Gaps = 1/278 (0%) Query: 1 VTKGQPLMEIYSPAISSAAAEYVATITSKATVGIEPYGRGSKQRLTNLDVPEPVIAEMEK 60 V+ GQPLMEIYS AI++AAAEY+ATI SK G+E YGRGS+QRLTNLDVPEPVI MEK Sbjct: 198 VSAGQPLMEIYSSAIAAAAAEYLATINSKIVGGVEFYGRGSRQRLTNLDVPEPVITAMEK 257 Query: 61 SRTVPIAIQWLSPRDGIVLQRAAIEGMRAQPGDVLFRIADVSVVWALVDVAERDLGNIVV 120 S P+ +QW +PRDGIVL+R A+EGMRA PGDVLFRIAD+S+VWALVDVAERDLG + V Sbjct: 258 SHVAPVTVQWTAPRDGIVLERNAVEGMRANPGDVLFRIADISLVWALVDVAERDLGTVAV 317 Query: 121 GQKVTVRARSYPGRSFAGQISVIYPQVNKETRTARVRIELQNADLALLPDMYVDAEIDTG 180 GQ V +RARS+ GR+F+G+I+VIYPQVN++TRTARVRIEL N D+ALLPDMYVDAEI+T Sbjct: 318 GQPVLIRARSFAGRTFSGKIAVIYPQVNRDTRTARVRIELSNPDVALLPDMYVDAEINTA 377 Query: 181 GAAPVLTVADNSILDTGSRQSVLIDRGNGRFEPREVKLGRRGEGYVEIRDGLAEGDAVVT 240 A+ V+ V D+++LDTG+RQ+VL+D+G G+FEPR+VKLGRRG GY+EIR GL EG+AVVT Sbjct: 378 DASSVVAVPDSAVLDTGARQAVLVDKGEGKFEPRDVKLGRRGGGYIEIRGGLKEGEAVVT 437 Query: 241 SATFLIDAESNLKAAIKGFAEAG-AAQAGEGEKTGDRR 277 SA FLIDAESNLKAA++GFA+A ++ A G G+ + Sbjct: 438 SANFLIDAESNLKAALRGFADAHPSSDAAGGHAHGEHK 475