BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0134.p (221 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_004139906.1| HAD-superfamily hydrolase [Mesorhizobium cic... 304 8e-81 ref|YP_004609287.1| HAD-superfamily hydrolase [Mesorhizobium opp... 301 4e-80 ref|ZP_07478026.1| HAD-superfamily hydrolase, subfamily IA, vari... 285 4e-75 ref|NP_541106.1| hydrolase family protein [Brucella melitensis b... 283 1e-74 ref|YP_002278432.1| HAD-superfamily hydrolase [Rhizobium legumin... 283 2e-74 >ref|YP_004139906.1| HAD-superfamily hydrolase [Mesorhizobium ciceri biovar biserrulae WSM1271] gb|ADV09856.1| HAD-superfamily hydrolase, subfamily IA, variant 3 [Mesorhizobium ciceri biovar biserrulae WSM1271] Length = 222 Score = 304 bits (778), Expect = 8e-81, Method: Compositional matrix adjust. Identities = 149/213 (69%), Positives = 168/213 (78%) Query: 6 AQALVLDFGGVISKTLFETHDLTERTLGLAPCTLKWLGPFAPDSDPLWTSMQRGEISERD 65 AQALVLDFGGV+++TLFETH LTE+ LGLAP TL+W GPF P SDPLW +MQ EISERD Sbjct: 6 AQALVLDFGGVVTRTLFETHALTEQALGLAPGTLQWRGPFDPGSDPLWRAMQADEISERD 65 Query: 66 YWLTRSREVGRLVGEDWRDMETFVKRARGADPDTVIRPEAARAIALAADAGFRLAILSNE 125 YW TR+ EVGRLVGEDW+ METFV+RARGA+P+ V+RPEA I AGFRLAILSNE Sbjct: 66 YWRTRTSEVGRLVGEDWQAMETFVRRARGAEPEKVVRPEADSTIRTVHAAGFRLAILSNE 125 Query: 126 LDLFYGADFRSRLSILKRFDTIVDATHTGILKPDPRAYQSVLDELALEADNCVFVDDQRR 185 LDLFYGADFR RL +L FD I+DAT+TGILKPDPRAY V + L L A CVFVDDQRR Sbjct: 126 LDLFYGADFRQRLPLLGLFDVIIDATYTGILKPDPRAYAFVTEALGLPASACVFVDDQRR 185 Query: 186 NIAGAAACNMRTVLFDVRNPSASYSEALRHFGL 218 N+ G A MRTV FDV P+ SY+EAL HF L Sbjct: 186 NVDGGCAAGMRTVHFDVARPAHSYAEALGHFDL 218 >ref|YP_004609287.1| HAD-superfamily hydrolase [Mesorhizobium opportunistum WSM2075] gb|AEH85193.1| HAD-superfamily hydrolase, subfamily IA, variant 3 [Mesorhizobium opportunistum WSM2075] Length = 222 Score = 301 bits (772), Expect = 4e-80, Method: Compositional matrix adjust. Identities = 150/218 (68%), Positives = 169/218 (77%) Query: 1 MRRQLAQALVLDFGGVISKTLFETHDLTERTLGLAPCTLKWLGPFAPDSDPLWTSMQRGE 60 M LA+ALVLDFGGV+++TLFETH LTE+ LGL P TL+W GPF P SDPLW +MQ E Sbjct: 1 MSAVLAKALVLDFGGVVTRTLFETHALTEQALGLKPGTLQWRGPFDPASDPLWRTMQADE 60 Query: 61 ISERDYWLTRSREVGRLVGEDWRDMETFVKRARGADPDTVIRPEAARAIALAADAGFRLA 120 ISERDYW TR+ EVGRLVGEDW+ METFV+RARGA+P+ V+RPEA AI AGFRLA Sbjct: 61 ISERDYWRTRTSEVGRLVGEDWQAMETFVQRARGAEPEKVVRPEADSAIRAVHAAGFRLA 120 Query: 121 ILSNELDLFYGADFRSRLSILKRFDTIVDATHTGILKPDPRAYQSVLDELALEADNCVFV 180 ILSNELDLFYGA FR +L +L FD IVDATHTGILKPDPRAY V + L L A CVFV Sbjct: 121 ILSNELDLFYGAGFRQKLPLLGLFDVIVDATHTGILKPDPRAYAFVTEALGLPAGACVFV 180 Query: 181 DDQRRNIAGAAACNMRTVLFDVRNPSASYSEALRHFGL 218 DDQ+RNI G A MRTV FDV P+ SY+EAL HF L Sbjct: 181 DDQQRNIDGGRAAGMRTVHFDVARPAFSYAEALGHFDL 218 >ref|ZP_07478026.1| HAD-superfamily hydrolase, subfamily IA, variant 3 [Brucella sp. BO1] gb|EFM56011.1| HAD-superfamily hydrolase, subfamily IA, variant 3 [Brucella sp. BO1] Length = 214 Score = 285 bits (729), Expect = 4e-75, Method: Compositional matrix adjust. Identities = 132/214 (61%), Positives = 170/214 (79%) Query: 5 LAQALVLDFGGVISKTLFETHDLTERTLGLAPCTLKWLGPFAPDSDPLWTSMQRGEISER 64 +++AL+LDFGGV+++TLFETHD+TER LGL +L W GPF P +DPLW SMQ EI+ER Sbjct: 1 MSKALILDFGGVVTRTLFETHDITERALGLPAGSLGWKGPFDPSTDPLWVSMQAREITER 60 Query: 65 DYWLTRSREVGRLVGEDWRDMETFVKRARGADPDTVIRPEAARAIALAADAGFRLAILSN 124 DYW+TR+RE G L+GE+W DM+TFV+RARGA+P+ V+RPEA AI A AG +LAILSN Sbjct: 61 DYWMTRTRETGALLGENWTDMKTFVQRARGAEPELVLRPEARDAILRAKAAGLKLAILSN 120 Query: 125 ELDLFYGADFRSRLSILKRFDTIVDATHTGILKPDPRAYQSVLDELALEADNCVFVDDQR 184 ELDLFYG +FR R +++ FD IVDAT+T ILKPDPRAY+ VL ELAL+ +CVFVDDQ+ Sbjct: 121 ELDLFYGVEFRKRFPLIELFDVIVDATYTKILKPDPRAYEQVLAELALDRADCVFVDDQK 180 Query: 185 RNIAGAAACNMRTVLFDVRNPSASYSEALRHFGL 218 +NI GA A ++ V FDV P+ SY++AL+ G+ Sbjct: 181 KNIEGAEAVHLPHVHFDVTRPAESYAQALKMLGI 214 >ref|NP_541106.1| hydrolase family protein [Brucella melitensis bv. 1 str. 16M] ref|NP_700320.1| HAD superfamily hydrolase [Brucella suis 1330] ref|YP_001595096.1| HAD family hydrolase [Brucella canis ATCC 23365] ref|ZP_03786488.1| HAD-superfamily hydrolase, subfamily IA, variant 3 [Brucella ceti str. Cudo] ref|YP_002734882.1| HAD-superfamily hydrolase [Brucella melitensis ATCC 23457] ref|YP_003105935.1| haloacid dehalogenase-like family hydrolase [Brucella microti CCM 4915] ref|ZP_05464484.1| hydrolase [Brucella melitensis bv. 2 str. 63/9] ref|ZP_05834615.1| hydrolase [Brucella melitensis bv. 1 str. 16M] ref|ZP_05839156.1| hydrolase [Brucella suis bv. 4 str. 40] ref|ZP_05934121.1| hydrolase [Brucella ceti B1/94] ref|ZP_05953119.1| hydrolase [Brucella pinnipedialis M163/99/10] ref|ZP_05958035.1| hydrolase [Brucella pinnipedialis B2/94] ref|ZP_05962954.1| hydrolase [Brucella neotomae 5K33] ref|ZP_05996682.1| hydrolase [Brucella suis bv. 3 str. 686] ref|ZP_05999909.1| hydrolase [Brucella sp. F5/99] ref|ZP_06099713.1| hydrolase [Brucella pinnipedialis M292/94/1] ref|ZP_06101886.1| hydrolase [Brucella melitensis bv. 1 str. Rev.1] ref|ZP_06106130.1| hydrolase [Brucella melitensis bv. 3 str. Ether] ref|ZP_06109375.1| hydrolase [Brucella ceti M490/95/1] ref|ZP_06793669.1| HAD superfamily protein [Brucella sp. NVSL 07-0026] ref|YP_004758386.1| HAD superfamily hydrolase [Brucella pinnipedialis B2/94] gb|AAL53370.1| hydrolase family protein [Brucella melitensis bv. 1 str. 16M] gb|AAN34325.1| hydrolase, haloacid dehalogenase-like family [Brucella suis 1330] gb|ABX64325.1| HAD-superfamily hydrolase, subfamily IA, variant 3 [Brucella canis ATCC 23365] gb|EEH13348.1| HAD-superfamily hydrolase, subfamily IA, variant 3 [Brucella ceti str. Cudo] gb|ACO02928.1| HAD-superfamily hydrolase, subfamily IA, variant 3 [Brucella melitensis ATCC 23457] gb|ACU50273.1| hydrolase, haloacid dehalogenase-like family [Brucella microti CCM 4915] gb|EEW86866.1| hydrolase [Brucella melitensis bv. 1 str. 16M] gb|EEW90433.1| hydrolase [Brucella suis bv. 4 str. 40] gb|EEX85077.1| hydrolase [Brucella ceti B1/94] gb|EEY01558.1| hydrolase [Brucella pinnipedialis B2/94] gb|EEY03234.1| hydrolase [Brucella neotomae 5K33] gb|EEY06445.1| hydrolase [Brucella pinnipedialis M163/99/10] gb|EEY24180.1| hydrolase [Brucella sp. F5/99] gb|EEY30652.1| hydrolase [Brucella suis bv. 3 str. 686] gb|EEZ07276.1| hydrolase [Brucella ceti M490/95/1] gb|EEZ10475.1| hydrolase [Brucella melitensis bv. 3 str. Ether] gb|EEZ12688.1| hydrolase [Brucella melitensis bv. 1 str. Rev.1] gb|EEZ15968.1| hydrolase [Brucella melitensis bv. 2 str. 63/9] gb|EEZ29614.1| hydrolase [Brucella pinnipedialis M292/94/1] gb|EFG35652.1| HAD superfamily protein [Brucella sp. NVSL 07-0026] gb|ADZ68399.1| HAD-superfamily hydrolase [Brucella melitensis M28] gb|ADZ89263.1| HAD-superfamily hydrolase [Brucella melitensis M5-90] gb|AEK56618.1| HAD superfamily hydrolase [Brucella pinnipedialis B2/94] gb|AEM20601.1| HAD superfamily hydrolase [Brucella suis 1330] Length = 214 Score = 283 bits (725), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 131/214 (61%), Positives = 169/214 (78%) Query: 5 LAQALVLDFGGVISKTLFETHDLTERTLGLAPCTLKWLGPFAPDSDPLWTSMQRGEISER 64 +++AL+LDFGGV+++TLFETHD+TER LGL +L W GPF P +DPLW SMQ EI+ER Sbjct: 1 MSKALILDFGGVVTRTLFETHDITERALGLPAGSLGWKGPFDPSTDPLWVSMQAREITER 60 Query: 65 DYWLTRSREVGRLVGEDWRDMETFVKRARGADPDTVIRPEAARAIALAADAGFRLAILSN 124 DYW+TR+RE G L+GE+W DM+TFV+RARGA+P+ V+RPEA AI AG +LAILSN Sbjct: 61 DYWMTRTRETGALLGENWTDMKTFVQRARGAEPELVLRPEARDAILRTKAAGLKLAILSN 120 Query: 125 ELDLFYGADFRSRLSILKRFDTIVDATHTGILKPDPRAYQSVLDELALEADNCVFVDDQR 184 ELDLFYG +FR R +++ FD IVDAT+T ILKPDPRAY+ VL ELAL+ +CVFVDDQ+ Sbjct: 121 ELDLFYGVEFRKRFPLIELFDVIVDATYTKILKPDPRAYEQVLAELALDRADCVFVDDQK 180 Query: 185 RNIAGAAACNMRTVLFDVRNPSASYSEALRHFGL 218 +NI GA A ++ V FDV P+ SY++AL+ G+ Sbjct: 181 KNIEGAEAVHLPHVHFDVTRPAESYAQALKMLGI 214 >ref|YP_002278432.1| HAD-superfamily hydrolase [Rhizobium leguminosarum bv. trifolii WSM2304] gb|ACI57692.1| HAD-superfamily hydrolase, subfamily IA, variant 3 [Rhizobium leguminosarum bv. trifolii WSM2304] Length = 216 Score = 283 bits (723), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 133/212 (62%), Positives = 166/212 (78%) Query: 7 QALVLDFGGVISKTLFETHDLTERTLGLAPCTLKWLGPFAPDSDPLWTSMQRGEISERDY 66 +AL+LDFGGV+++TLFETH++TERTLGL +L WLGPF +DPLW +MQ EI+ERDY Sbjct: 5 RALILDFGGVVTRTLFETHEITERTLGLPQGSLTWLGPFDTVTDPLWVAMQNREITERDY 64 Query: 67 WLTRSREVGRLVGEDWRDMETFVKRARGADPDTVIRPEAARAIALAADAGFRLAILSNEL 126 WLTR+ E+GR+VGE+W DM+TFV+RARGA+P+ V+RPEA AI A +AG +LAILSNEL Sbjct: 65 WLTRAAEIGRMVGENWSDMQTFVRRARGAEPELVLRPEARDAILRAKEAGLKLAILSNEL 124 Query: 127 DLFYGADFRSRLSILKRFDTIVDATHTGILKPDPRAYQSVLDELALEADNCVFVDDQRRN 186 DLFYGA+FR R ++ FD IVDAT+T ILKPDPRAY+ VL EL L + CVFVDDQ++N Sbjct: 125 DLFYGAEFRKRFPLIDLFDVIVDATYTKILKPDPRAYEQVLSELGLPREACVFVDDQKKN 184 Query: 187 IAGAAACNMRTVLFDVRNPSASYSEALRHFGL 218 I GA A + V FDV P+ SY+ AL GL Sbjct: 185 IEGAEAVGLPHVHFDVTRPAESYARALAMLGL 216