BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0107.p (312 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_571412.1| amidase [Rhodopseudomonas palustris BisB5] >gi|... 503 e-140 ref|YP_001206373.1| putative amidase [Bradyrhizobium sp. ORS 278... 494 e-138 ref|YP_004234911.1| amidase [Acidovorax avenae subsp. avenae ATC... 384 e-104 ref|ZP_03264706.1| Amidase [Burkholderia sp. H160] >gi|209503692... 373 e-101 ref|YP_004486692.1| Amidase [Delftia sp. Cs1-4] >gi|333743160|gb... 371 e-101 >ref|YP_571412.1| amidase [Rhodopseudomonas palustris BisB5] gb|ABE41511.1| Amidase [Rhodopseudomonas palustris BisB5] Length = 466 Score = 503 bits (1294), Expect = e-140, Method: Compositional matrix adjust. Identities = 257/312 (82%), Positives = 282/312 (90%), Gaps = 2/312 (0%) Query: 1 MPTTDLHYLGLIEIGQQIQAKKLSPVEVTKAMLGRIEQLDGKLKSYAYVMGEAALAEAAA 60 M TTDLHY GL+E+G++IQA KLSPVEVTKAML RIE+LDGKLKSYAYV E AL EAA+ Sbjct: 1 MATTDLHYRGLVEVGREIQANKLSPVEVTKAMLQRIEKLDGKLKSYAYVTPELALEEAAS 60 Query: 61 AEKDIASGKIKGPLHGVPIAVKDLCWAKGAPAAHGMTIHRDFRPSDDATVVARLKDAGAI 120 AEK+IA+GK+KGPLHGVPIAVKDLCW KG PAAHGMTIHRD+RPS+DATVVARLK+AGAI Sbjct: 61 AEKEIAAGKVKGPLHGVPIAVKDLCWVKGVPAAHGMTIHRDYRPSEDATVVARLKNAGAI 120 Query: 121 ILGKLQQTEGAYADHHPKIDPPKNPWNADLWSGASSSGSGVATAAGLCFGSLGTDTGGSI 180 ILGKLQQTEGAYADHHPKIDPPKNPWN +LWSG SSSGSG ATAAGLCFGSLGTDTGGSI Sbjct: 121 ILGKLQQTEGAYADHHPKIDPPKNPWNDELWSGVSSSGSGSATAAGLCFGSLGTDTGGSI 180 Query: 181 RFPSAANGITGLKPTWGRVSRYGAFELAATLDHIGPMARSAADCGAILAVIAGQDPKDTT 240 RFPSAANG+TGLKPTWGRVSRYGA+ELAATLDHIGPMARSA DCGA+L+VIAG DPKDTT Sbjct: 181 RFPSAANGVTGLKPTWGRVSRYGAYELAATLDHIGPMARSAIDCGAMLSVIAGADPKDTT 240 Query: 241 SVPLPVPNYLAGLSGDLRGVTIGVDRRWTSEGTDEAAAKVLSEGLRVAADLGAKIKDITF 300 +VPLPVPNY+A L GDL+G+ IGVDRRWTSEGTDEAA KV + LRVA DLGA I +I F Sbjct: 241 AVPLPVPNYVANLPGDLKGMVIGVDRRWTSEGTDEAATKVFEDALRVAKDLGATITEIVF 300 Query: 301 PDPKAVF--FFP 310 PD +AV +FP Sbjct: 301 PDAQAVTDDWFP 312 >ref|YP_001206373.1| putative amidase [Bradyrhizobium sp. ORS 278] emb|CAL78150.1| putative amidase [Bradyrhizobium sp. ORS 278] Length = 466 Score = 494 bits (1272), Expect = e-138, Method: Compositional matrix adjust. Identities = 263/312 (84%), Positives = 293/312 (93%), Gaps = 2/312 (0%) Query: 1 MPTTDLHYLGLIEIGQQIQAKKLSPVEVTKAMLGRIEQLDGKLKSYAYVMGEAALAEAAA 60 MP TDLHYLGL+++G++IQAK+LSPVEVTKAML RIE+LDG+LKS+AYVM ++A+A+AAA Sbjct: 1 MPATDLHYLGLVDVGRKIQAKELSPVEVTKAMLERIEKLDGQLKSFAYVMADSAVADAAA 60 Query: 61 AEKDIASGKIKGPLHGVPIAVKDLCWAKGAPAAHGMTIHRDFRPSDDATVVARLKDAGAI 120 AEK+IASGKI+GPLHGVP+AVKDLCWAKGAPAAHGMTIHRDFRP++DATVVARLK AGA+ Sbjct: 61 AEKEIASGKIRGPLHGVPVAVKDLCWAKGAPAAHGMTIHRDFRPNEDATVVARLKAAGAV 120 Query: 121 ILGKLQQTEGAYADHHPKIDPPKNPWNADLWSGASSSGSGVATAAGLCFGSLGTDTGGSI 180 ILGKLQQTEGAYADHHPKIDPPKNPWNADLW GASSSGSG ATAAGLCFGSLGTDTGGSI Sbjct: 121 ILGKLQQTEGAYADHHPKIDPPKNPWNADLWPGASSSGSGAATAAGLCFGSLGTDTGGSI 180 Query: 181 RFPSAANGITGLKPTWGRVSRYGAFELAATLDHIGPMARSAADCGAILAVIAGQDPKDTT 240 RFPSAANG+TGLKPTWGRVSRYGAFELAATLDHIGPMARSAADCGA+L VIAGQDP+DTT Sbjct: 181 RFPSAANGVTGLKPTWGRVSRYGAFELAATLDHIGPMARSAADCGAVLGVIAGQDPRDTT 240 Query: 241 SVPLPVPNYLAGLSGDLRGVTIGVDRRWTSEGTDEAAAKVLSEGLRVAADLGAKIKDITF 300 +VPLPVP+YLA LSGDLRGVTIG+DRRWTSEGTD A VL+E LRVA DLGA+IK+I F Sbjct: 241 AVPLPVPDYLASLSGDLRGVTIGIDRRWTSEGTDSDTANVLTEALRVAVDLGAQIKEINF 300 Query: 301 PDPKAVF--FFP 310 PD +AV +FP Sbjct: 301 PDSQAVIDDWFP 312 >ref|YP_004234911.1| amidase [Acidovorax avenae subsp. avenae ATCC 19860] gb|ADX46344.1| Amidase [Acidovorax avenae subsp. avenae ATCC 19860] Length = 466 Score = 384 bits (985), Expect = e-104, Method: Compositional matrix adjust. Identities = 190/302 (62%), Positives = 237/302 (78%) Query: 1 MPTTDLHYLGLIEIGQQIQAKKLSPVEVTKAMLGRIEQLDGKLKSYAYVMGEAALAEAAA 60 M T+LHYL L+E+G+QIQ+++ S +EVT+AML RI ++D KL SYA +M + AL +A Sbjct: 1 MAHTELHYLELLEVGRQIQSRERSSLEVTQAMLERIGRIDAKLHSYATLMADHALRDARQ 60 Query: 61 AEKDIASGKIKGPLHGVPIAVKDLCWAKGAPAAHGMTIHRDFRPSDDATVVARLKDAGAI 120 A+ DIA+ + +GPLHGVPIAVKDL W +G P+AHGMTIHRD+RP +DAT V RL++AGA+ Sbjct: 61 ADADIAANRARGPLHGVPIAVKDLLWTQGVPSAHGMTIHRDYRPQEDATAVRRLREAGAV 120 Query: 121 ILGKLQQTEGAYADHHPKIDPPKNPWNADLWSGASSSGSGVATAAGLCFGSLGTDTGGSI 180 +LGKLQQTEGA+ADHHP++ P NPW LW GASSSGSGVATAAGLCF SLGTDTGGSI Sbjct: 121 LLGKLQQTEGAFADHHPQVVAPLNPWGDTLWPGASSSGSGVATAAGLCFASLGTDTGGSI 180 Query: 181 RFPSAANGITGLKPTWGRVSRYGAFELAATLDHIGPMARSAADCGAILAVIAGQDPKDTT 240 RFPSAANG+TGLKPTW RVSR+GAFELAA+LDHIGP+ARSAAD A+L VIAG DPKD T Sbjct: 181 RFPSAANGVTGLKPTWSRVSRHGAFELAASLDHIGPIARSAADAAAVLQVIAGADPKDPT 240 Query: 241 SVPLPVPNYLAGLSGDLRGVTIGVDRRWTSEGTDEAAAKVLSEGLRVAADLGAKIKDITF 300 + PVP+YLA ++ L G+ +G+DR WT + D VL L + LG ++++ F Sbjct: 241 ASQEPVPDYLAQMTRGLNGLRVGIDRAWTLDRADAPTRAVLQSVLELVEPLGGSLREVDF 300 Query: 301 PD 302 PD Sbjct: 301 PD 302 >ref|ZP_03264706.1| Amidase [Burkholderia sp. H160] gb|EEA03686.1| Amidase [Burkholderia sp. H160] Length = 465 Score = 373 bits (957), Expect = e-101, Method: Compositional matrix adjust. Identities = 183/306 (59%), Positives = 232/306 (75%), Gaps = 1/306 (0%) Query: 1 MPTTDLHYLGLIEIGQQIQAKKLSPVEVTKAMLGRIEQLDGKLKSYAYVMGEAALAEAAA 60 M TDLHYL + + + I+ +++SPVEVT+AML RIE+LD L SYA VM E AL +AA Sbjct: 1 MSNTDLHYLDIGTLARMIRTREISPVEVTRAMLERIERLDPTLHSYALVMRERALVDAAE 60 Query: 61 AEKDIASGKIKGPLHGVPIAVKDLCWAKGAPAAHGMTIHRDFRPSDDATVVARLKDAGAI 120 AE+ + +G+ G LHGVP+ VKDL W G P A G TIH++F P++DATVV RL++AGA+ Sbjct: 61 AERKVLAGETLGALHGVPLGVKDLFWTAGTPTAAGTTIHKNFVPNEDATVVRRLREAGAV 120 Query: 121 ILGKLQQTEGAYADHHPKIDPPKNPWNADLWSGASSSGSGVATAAGLCFGSLGTDTGGSI 180 +LGKLQ TEGA+A HHP I P NPW+A+ W+GASSSGSGVATAAGLC+G+LGTDTGGSI Sbjct: 121 VLGKLQMTEGAFATHHPSIAEPVNPWHANHWAGASSSGSGVATAAGLCYGALGTDTGGSI 180 Query: 181 RFPSAANGITGLKPTWGRVSRYGAFELAATLDHIGPMARSAADCGAILAVIAGQDPKDTT 240 RFP+AANG+TG+KPTWGRVSRYGAFEL A+LDH+GPMAR+AAD +L VIAG DP D T Sbjct: 181 RFPAAANGLTGIKPTWGRVSRYGAFELGASLDHVGPMARNAADAALMLGVIAGYDPLDPT 240 Query: 241 SVPLPVPNYLAGLSGDLRGVTIGVDRRWTSEGTDEAAAKVLSEGLRVAADLGAKIKDITF 300 S+P + L+G +GDLRG+ IG+D W +EGTD + L L V +LGA I+++ Sbjct: 241 SLPEERLS-LSGQAGDLRGLRIGIDPAWNTEGTDAVIVEALERVLMVLKELGADIREVRV 299 Query: 301 PDPKAV 306 P + V Sbjct: 300 PAVRTV 305 >ref|YP_004486692.1| Amidase [Delftia sp. Cs1-4] gb|AEF88337.1| Amidase [Delftia sp. Cs1-4] Length = 466 Score = 371 bits (952), Expect = e-101, Method: Compositional matrix adjust. Identities = 187/302 (61%), Positives = 238/302 (78%) Query: 1 MPTTDLHYLGLIEIGQQIQAKKLSPVEVTKAMLGRIEQLDGKLKSYAYVMGEAALAEAAA 60 M ++LH+L L+E+G++IQ++++S EVT+ ML RIE +D +L SY VM + AL +A Sbjct: 1 MNDSELHHLELLEVGREIQSRRISSEEVTRHMLARIEAVDARLHSYVTVMAQQALEDARR 60 Query: 61 AEKDIASGKIKGPLHGVPIAVKDLCWAKGAPAAHGMTIHRDFRPSDDATVVARLKDAGAI 120 A+ +IA G+ +G LHGVP+A+KDL W +G P HGMT+HRD RP++DATVV RL++AGA+ Sbjct: 61 ADAEIAQGRRRGALHGVPLALKDLLWTRGVPTTHGMTLHRDHRPTEDATVVRRLREAGAV 120 Query: 121 ILGKLQQTEGAYADHHPKIDPPKNPWNADLWSGASSSGSGVATAAGLCFGSLGTDTGGSI 180 ILGKLQQTEGA+ADHHP+I P NPW A LW GASSSGSGVATAAGLCFGSLGTDTGGSI Sbjct: 121 ILGKLQQTEGAFADHHPEITAPVNPWGAQLWPGASSSGSGVATAAGLCFGSLGTDTGGSI 180 Query: 181 RFPSAANGITGLKPTWGRVSRYGAFELAATLDHIGPMARSAADCGAILAVIAGQDPKDTT 240 RFPSAANGITGLKPTWGRVSR+GAFELAA+LDHIGPMARSAAD A+LA IAG DP D T Sbjct: 181 RFPSAANGITGLKPTWGRVSRHGAFELAASLDHIGPMARSAADAAAMLAAIAGADPLDPT 240 Query: 241 SVPLPVPNYLAGLSGDLRGVTIGVDRRWTSEGTDEAAAKVLSEGLRVAADLGAKIKDITF 300 + VP+YLA ++ G+ +G+DR+W +G D + + + + L VA LGA ++++ F Sbjct: 241 ASQCSVPDYLAMMTRGFSGLRLGMDRQWALDGVDAPSRQAVEQALAVAQRLGASVQEVRF 300 Query: 301 PD 302 PD Sbjct: 301 PD 302