BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0070.p (168 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_07032272.1| protein of unknown function DUF417 [Acidobact... 227 4e-58 ref|YP_004675573.1| hypothetical protein HYPMC_1776 [Hyphomicrob... 196 9e-49 ref|YP_001583934.1| hypothetical protein Bmul_3959 [Burkholderia... 94 8e-18 ref|YP_001948930.1| probable membrane spanning protein [Burkhold... 93 1e-17 ref|ZP_03569099.1| hypothetical membrane spanning protein [Burkh... 91 4e-17 >ref|ZP_07032272.1| protein of unknown function DUF417 [Acidobacterium sp. MP5ACTX8] gb|EFI54938.1| protein of unknown function DUF417 [Acidobacterium sp. MP5ACTX8] Length = 164 Score = 227 bits (579), Expect = 4e-58, Method: Compositional matrix adjust. Identities = 110/156 (70%), Positives = 130/156 (83%) Query: 10 NPLARALDRSGLLAEDLDYHVVRAAMVIMFLFFGYQKWFPYEFERLVPFISNGPLIWWLY 69 N L R L ++GLL +LD H+VRA+MVI++ FFGYQKWF YE + LVPF ++GPLI W+Y Sbjct: 2 NWLIRLLSKTGLLKGNLDNHLVRASMVIIYFFFGYQKWFDYEVKGLVPFFTHGPLISWMY 61 Query: 70 PAFGHAGASYFLGASEWTFGSLLLAGFWDKRLGVLGALGSTGTFIATVTIIPFMPDGWDA 129 P FG G+SYFLG SEW FG+LLLAGFW+K+LGVLGALGS TFIATVTIIPFMPDGW Sbjct: 62 PVFGMTGSSYFLGVSEWLFGALLLAGFWNKKLGVLGALGSVFTFIATVTIIPFMPDGWAP 121 Query: 130 AAGGFPAMTGNVPFLMKDVVLLAVSLYLLRQDVVRL 165 +AGGFPAM GNV FLMKDVVLLAVS YLL+QD+ ++ Sbjct: 122 SAGGFPAMVGNVAFLMKDVVLLAVSFYLLKQDLAKV 157 >ref|YP_004675573.1| hypothetical protein HYPMC_1776 [Hyphomicrobium sp. MC1] emb|CCB65001.1| conserved membrane protein of unknown function [Hyphomicrobium sp. MC1] Length = 161 Score = 196 bits (498), Expect = 9e-49, Method: Compositional matrix adjust. Identities = 97/159 (61%), Positives = 125/159 (78%), Gaps = 2/159 (1%) Query: 10 NPLARALDRSGLLAEDLDYHVVRAAMVIMFLFFGYQKWFPYEFERLVPFISNGPLIWWLY 69 N +A+ L R+GLL LDY+++RAAMVI+F +FGY KWF E L+P I++GP I+W Sbjct: 2 NVIAQLLGRTGLL--KLDYNLLRAAMVIIFAWFGYDKWFEAEIRGLLPIITHGPFIFWTI 59 Query: 70 PAFGHAGASYFLGASEWTFGSLLLAGFWDKRLGVLGALGSTGTFIATVTIIPFMPDGWDA 129 P G G S FLGASEWTFG+LL GFW+K+LG+LGALGST TFIAT T++PF+PD WDA Sbjct: 60 PVLGIHGTSVFLGASEWTFGTLLFLGFWNKKLGMLGALGSTFTFIATFTVLPFVPDAWDA 119 Query: 130 AAGGFPAMTGNVPFLMKDVVLLAVSLYLLRQDVVRLIRQ 168 AGGFPAMT N FL+KD+VLLAVS+Y+L++DV R++ + Sbjct: 120 GAGGFPAMTINSAFLLKDLVLLAVSVYILKEDVQRVLNE 158 >ref|YP_001583934.1| hypothetical protein Bmul_3959 [Burkholderia multivorans ATCC 17616] gb|ABX17642.1| protein of unknown function DUF417 [Burkholderia multivorans ATCC 17616] Length = 200 Score = 94.0 bits (232), Expect = 8e-18, Method: Compositional matrix adjust. Identities = 65/165 (39%), Positives = 95/165 (57%), Gaps = 10/165 (6%) Query: 1 MTTLANTTHNPLA-RALDRSGLLAEDLDYHVVRAAMVIMFLFFGYQKWFPYEFERLVPFI 59 M+T AN H +A + + LLA ++R A+V++FL+FG K+ YE + PF+ Sbjct: 33 MSTRANQFHLQIAEKGVGSLSLLA------LLRWALVVIFLWFGGMKFTGYEANGIAPFV 86 Query: 60 SNGPLIWWLYPAFGHAGASYFLGASEWTFGSLLLAGFWDKRLGVLGALGSTGTFIATVTI 119 N P++ WL +FG GAS +G E + + L+AG + L LGA S T++ T+T Sbjct: 87 VNSPIMSWLNASFGVQGASNVIGVLELSTAAALIAGAFQPVLSALGAAMSAATYLITLTF 146 Query: 120 IPFMPDGWDAAAGGFPAMTGNVP--FLMKDVVLLAVSLYLLRQDV 162 P +A AGGFPA++ +P FL+KD+VLLA SL LL V Sbjct: 147 FLSTPGVAEATAGGFPAISA-LPGQFLLKDLVLLAASLCLLVASV 190 >ref|YP_001948930.1| probable membrane spanning protein [Burkholderia multivorans ATCC 17616] dbj|BAG46394.1| probable membrane spanning protein [Burkholderia multivorans ATCC 17616] Length = 168 Score = 93.2 bits (230), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 65/165 (39%), Positives = 95/165 (57%), Gaps = 10/165 (6%) Query: 1 MTTLANTTHNPLA-RALDRSGLLAEDLDYHVVRAAMVIMFLFFGYQKWFPYEFERLVPFI 59 M+T AN H +A + + LLA ++R A+V++FL+FG K+ YE + PF+ Sbjct: 1 MSTRANQFHLQIAEKGVGSLSLLA------LLRWALVVIFLWFGGMKFTGYEANGIAPFV 54 Query: 60 SNGPLIWWLYPAFGHAGASYFLGASEWTFGSLLLAGFWDKRLGVLGALGSTGTFIATVTI 119 N P++ WL +FG GAS +G E + + L+AG + L LGA S T++ T+T Sbjct: 55 VNSPIMSWLNASFGVQGASNVIGVLELSTAAALIAGAFQPVLSALGAAMSAATYLITLTF 114 Query: 120 IPFMPDGWDAAAGGFPAMTGNVP--FLMKDVVLLAVSLYLLRQDV 162 P +A AGGFPA++ +P FL+KD+VLLA SL LL V Sbjct: 115 FLSTPGVAEATAGGFPAISA-LPGQFLLKDLVLLAASLCLLVASV 158 >ref|ZP_03569099.1| hypothetical membrane spanning protein [Burkholderia multivorans CGD2M] ref|ZP_03575746.1| hypothetical membrane spanning protein [Burkholderia multivorans CGD2] gb|EEE09089.1| hypothetical membrane spanning protein [Burkholderia multivorans CGD2] gb|EEE15006.1| hypothetical membrane spanning protein [Burkholderia multivorans CGD2M] Length = 185 Score = 91.3 bits (225), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 56/135 (41%), Positives = 82/135 (60%), Gaps = 3/135 (2%) Query: 30 VVRAAMVIMFLFFGYQKWFPYEFERLVPFISNGPLIWWLYPAFGHAGASYFLGASEWTFG 89 ++R A+V++FL+FG K+ YE + PF+ N P++ WL +FG GAS +G E + Sbjct: 42 LLRWALVVIFLWFGGMKFTGYEANGIAPFVVNSPIMSWLNASFGVQGASNVIGVLELSTA 101 Query: 90 SLLLAGFWDKRLGVLGALGSTGTFIATVTIIPFMPDGWDAAAGGFPAMTGNVP--FLMKD 147 + L+AG + L LGA S T++ T+T P +A AGGFPA++ +P FL+KD Sbjct: 102 AALIAGAFQPVLSALGAAMSAATYLITLTFFLSTPGVAEATAGGFPAISA-LPGQFLLKD 160 Query: 148 VVLLAVSLYLLRQDV 162 +VLLA SL LL V Sbjct: 161 LVLLAASLCLLVASV 175