BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0033.p (333 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_002502162.1| HpcH/HpaI aldolase [Methylobacterium nodulan... 350 2e-94 ref|YP_001756322.1| Citryl-CoA lyase [Methylobacterium radiotole... 350 2e-94 ref|YP_001022442.1| Citryl-CoA lyase [Methylibium petroleiphilum... 348 8e-94 ref|YP_743876.1| malyl-CoA lyase [Granulibacter bethesdensis CGD... 345 7e-93 ref|YP_001773249.1| citrate (pro-3S)-lyase [Methylobacterium sp.... 345 8e-93 >ref|YP_002502162.1| HpcH/HpaI aldolase [Methylobacterium nodulans ORS 2060] gb|ACL61859.1| HpcH/HpaI aldolase [Methylobacterium nodulans ORS 2060] Length = 324 Score = 350 bits (897), Expect = 2e-94, Method: Compositional matrix adjust. Identities = 170/321 (52%), Positives = 224/321 (69%) Query: 1 MSFTVTKPRTRRVQRSELAVPATSTHFFERAASSAADFIFLDLEDAVAPPRKPEAREIAI 60 MSFT+ R+ RSELAVP ++ F E++A SAAD IFLDLEDAVAP K +AR+ I Sbjct: 1 MSFTLIPQAKARLHRSELAVPGSNPTFMEKSAQSAADVIFLDLEDAVAPDDKEQARKNII 60 Query: 61 EALNRIDWGNKTLAVRINGLDTEWAHRDIIEIVSRAPRLDLVMVPKVGTAFDVRFVDALL 120 +ALN IDWGNKT+ +RINGLDT + +RD+++IV PRLD++++PKVG DV +D L+ Sbjct: 61 QALNEIDWGNKTMMIRINGLDTHYMYRDVVDIVEACPRLDMILIPKVGVPADVYAIDVLV 120 Query: 121 TGLEREFPRARPLGIEILIETTLGLANVEDIVQASERLEAVIFGIGDYSIDLRTCGEIIG 180 T +E+ R + +G E+LIET LG+ANVE I Q+S+RLEA+ FG+ DY+ R +IG Sbjct: 121 TQIEQAKKREKKIGFEVLIETALGMANVEAIAQSSKRLEAMSFGVADYAASTRARSTVIG 180 Query: 181 ASDRRYAVLADAGEGGARRVHWNDQWHFALARIANACRAHGLRAIDGPYANFGDAAGFRA 240 + YAVL D G R HW D W FA R+ ACRA+GLR IDGP+ +F D GFRA Sbjct: 181 GVNPDYAVLTDKDAEGRRETHWADPWLFAQNRMLVACRAYGLRPIDGPFGDFSDPDGFRA 240 Query: 241 SAARAAALGFEGKWAIHPSQLETANEVFSPTAREIGWAREITAKIQSASAGGDGAFGQDG 300 +A R A LGFEGKWAIHPSQ+E ANEVF+PT E+ AR I ++ A+ G GA DG Sbjct: 241 AAKRCAVLGFEGKWAIHPSQIELANEVFTPTEAEVTKARRILQAMEEAAKAGRGAVSLDG 300 Query: 301 VLIDLAVLKRAQAIIDRNDAI 321 LID+A ++ A+A+I++ DA+ Sbjct: 301 RLIDIASIRMAEALIEKADAM 321 >ref|YP_001756322.1| Citryl-CoA lyase [Methylobacterium radiotolerans JCM 2831] gb|ACB25639.1| Citryl-CoA lyase [Methylobacterium radiotolerans JCM 2831] Length = 323 Score = 350 bits (897), Expect = 2e-94, Method: Compositional matrix adjust. Identities = 167/322 (51%), Positives = 230/322 (71%) Query: 1 MSFTVTKPRTRRVQRSELAVPATSTHFFERAASSAADFIFLDLEDAVAPPRKPEAREIAI 60 MSFT+ + R+ RSELAVP ++ F E++ASSAAD IFLDLEDAVAP K +AR I Sbjct: 1 MSFTLIQQAKPRLHRSELAVPGSNPTFMEKSASSAADVIFLDLEDAVAPDDKEKARTNII 60 Query: 61 EALNRIDWGNKTLAVRINGLDTEWAHRDIIEIVSRAPRLDLVMVPKVGTAFDVRFVDALL 120 +ALN +DWGNKT+ +RINGLDT + +RD+++IV PRLD+V++PKVG DV +D L+ Sbjct: 61 QALNDLDWGNKTMMIRINGLDTHYMYRDVVDIVEACPRLDMVLIPKVGVPADVYAIDVLV 120 Query: 121 TGLEREFPRARPLGIEILIETTLGLANVEDIVQASERLEAVIFGIGDYSIDLRTCGEIIG 180 T +E+ R + +G E+LIET LG+ANVE I Q+S+RLEA+ FG+ DY+ R +IG Sbjct: 121 TQIEQAKKREKQIGFEVLIETALGMANVEAIAQSSKRLEAMSFGVADYAASTRARSTVIG 180 Query: 181 ASDRRYAVLADAGEGGARRVHWNDQWHFALARIANACRAHGLRAIDGPYANFGDAAGFRA 240 + ++VL D E G+R+ HW D W FA R+ ACRA+GLR IDGP+ +F D G+R+ Sbjct: 181 GVNPDFSVLTDKDESGSRQTHWQDPWLFAQNRMLVACRAYGLRPIDGPFGDFSDPDGYRS 240 Query: 241 SAARAAALGFEGKWAIHPSQLETANEVFSPTAREIGWAREITAKIQSASAGGDGAFGQDG 300 +A R AALGFEGKWAIHPSQ+E ANEVF+P+ E+ AR I ++ A+ G GA DG Sbjct: 241 AARRCAALGFEGKWAIHPSQIELANEVFTPSEAEVTKARRILEAMEEAAKAGRGAVSLDG 300 Query: 301 VLIDLAVLKRAQAIIDRNDAIQ 322 LID+A ++ A+A+I++ +A++ Sbjct: 301 RLIDIASIRMAEALIEKANAMK 322 >ref|YP_001022442.1| Citryl-CoA lyase [Methylibium petroleiphilum PM1] gb|ABM96207.1| Citryl-CoA lyase [Methylibium petroleiphilum PM1] Length = 327 Score = 348 bits (892), Expect = 8e-94, Method: Compositional matrix adjust. Identities = 168/326 (51%), Positives = 227/326 (69%) Query: 1 MSFTVTKPRTRRVQRSELAVPATSTHFFERAASSAADFIFLDLEDAVAPPRKPEAREIAI 60 MSFT+ T R+ RSELAVP T+T FE+AA+SAAD +FLDLEDAVAP KP+AR+ I Sbjct: 1 MSFTIIDQATPRLHRSELAVPGTNTAMFEKAAASAADIVFLDLEDAVAPDDKPQARKNII 60 Query: 61 EALNRIDWGNKTLAVRINGLDTEWAHRDIIEIVSRAPRLDLVMVPKVGTAFDVRFVDALL 120 + LN +DWG KT+ +RINGLDT + +RD+++IV PRLD++++PK G A DV +D L+ Sbjct: 61 QGLNEVDWGTKTMMIRINGLDTHYMYRDVVDIVEACPRLDMILIPKAGVAADVYAIDMLV 120 Query: 121 TGLEREFPRARPLGIEILIETTLGLANVEDIVQASERLEAVIFGIGDYSIDLRTCGEIIG 180 T +E R++ +G E+LIET LG+ANVE I Q+S RLEA+ FG+ DY+ R +IG Sbjct: 121 TQIENAKGRSKRIGFEVLIETALGMANVEAIAQSSRRLEAMSFGVADYAASTRARTTVIG 180 Query: 181 ASDRRYAVLADAGEGGARRVHWNDQWHFALARIANACRAHGLRAIDGPYANFGDAAGFRA 240 ++ VL D E G R+ W D WH A R+ ACRA+GLR IDGP+ +FGD G+RA Sbjct: 181 GVNKDSVVLTDKDEQGNRQAFWTDPWHAAQTRMMVACRAYGLRPIDGPFGDFGDPDGYRA 240 Query: 241 SAARAAALGFEGKWAIHPSQLETANEVFSPTAREIGWAREITAKIQSASAGGDGAFGQDG 300 +A RAA LG+EGKWAIHPSQ+E AN VF+P+ E+ AR I + A+ G GA DG Sbjct: 241 AANRAAVLGYEGKWAIHPSQIELANGVFTPSEAEVTKARRILEAMAQAAKEGRGAVSLDG 300 Query: 301 VLIDLAVLKRAQAIIDRNDAIQAAGR 326 LID+A ++ A+A++ + D+I AA + Sbjct: 301 RLIDIASIRMAEALLAKADSISAAAK 326 >ref|YP_743876.1| malyl-CoA lyase [Granulibacter bethesdensis CGDNIH1] gb|ABI60953.1| malyl-CoA lyase [Granulibacter bethesdensis CGDNIH1] Length = 331 Score = 345 bits (884), Expect = 7e-93, Method: Compositional matrix adjust. Identities = 172/321 (53%), Positives = 229/321 (71%) Query: 1 MSFTVTKPRTRRVQRSELAVPATSTHFFERAASSAADFIFLDLEDAVAPPRKPEAREIAI 60 MSFT+ + T R+ RSELAVP ++T FE++A SAAD IFLD EDAVAP K +AR+ I Sbjct: 9 MSFTLVEQATPRLHRSELAVPGSNTALFEKSARSAADIIFLDCEDAVAPDEKDQARKNII 68 Query: 61 EALNRIDWGNKTLAVRINGLDTEWAHRDIIEIVSRAPRLDLVMVPKVGTAFDVRFVDALL 120 +ALN IDWGNKT+ VRINGLDT + +RD+++IV PRLD++++PKVGTA DV VD L+ Sbjct: 69 QALNEIDWGNKTMMVRINGLDTHYMYRDVVDIVEACPRLDMILIPKVGTAADVYAVDMLV 128 Query: 121 TGLEREFPRARPLGIEILIETTLGLANVEDIVQASERLEAVIFGIGDYSIDLRTCGEIIG 180 T +E+ R + +G E+LIET LG+ANVE I Q+S+RLEA+ FG+ DY+ R +IG Sbjct: 129 TQIEQAKKREKKIGFEVLIETALGMANVEAIAQSSKRLEAMSFGVADYAASTRARTTVIG 188 Query: 181 ASDRRYAVLADAGEGGARRVHWNDQWHFALARIANACRAHGLRAIDGPYANFGDAAGFRA 240 + Y VL D E G R +W DQWHFA +R+ ACRA+GLR IDGP+ +F D G+RA Sbjct: 189 GVNPDYGVLTDRDESGNRDYYWMDQWHFAQSRMMVACRAYGLRPIDGPFGDFNDPEGYRA 248 Query: 241 SAARAAALGFEGKWAIHPSQLETANEVFSPTAREIGWAREITAKIQSASAGGDGAFGQDG 300 +A RAA LG+EGKWAIHPSQ+ ANEVFSP+ E+ AR I ++ A+ G GA DG Sbjct: 249 AARRAAVLGYEGKWAIHPSQIALANEVFSPSEAEVTKARRILEAMEQAAKEGRGAVSLDG 308 Query: 301 VLIDLAVLKRAQAIIDRNDAI 321 LID+A ++ A+A++ + + I Sbjct: 309 RLIDIASIRMAEALLAKANQI 329 >ref|YP_001773249.1| citrate (pro-3S)-lyase [Methylobacterium sp. 4-46] gb|ACA20815.1| Citrate (pro-3S)-lyase [Methylobacterium sp. 4-46] Length = 323 Score = 345 bits (884), Expect = 8e-93, Method: Compositional matrix adjust. Identities = 167/322 (51%), Positives = 225/322 (69%) Query: 1 MSFTVTKPRTRRVQRSELAVPATSTHFFERAASSAADFIFLDLEDAVAPPRKPEAREIAI 60 MSFT+ R+ RSELAVP ++ F E++A SAAD IFLDLEDAVAP K +AR I Sbjct: 1 MSFTLIPQAKARLHRSELAVPGSNPTFMEKSAKSAADVIFLDLEDAVAPDDKEQARRNII 60 Query: 61 EALNRIDWGNKTLAVRINGLDTEWAHRDIIEIVSRAPRLDLVMVPKVGTAFDVRFVDALL 120 +ALN +DWG+KT+ +RINGLDT + +RD+++IV PRLD++++PKVG DV +D L+ Sbjct: 61 QALNDLDWGSKTMMIRINGLDTHYMYRDVVDIVEACPRLDMILIPKVGVPADVYAIDVLV 120 Query: 121 TGLEREFPRARPLGIEILIETTLGLANVEDIVQASERLEAVIFGIGDYSIDLRTCGEIIG 180 T +E+ R + +G E+LIET LG+ANVE I Q+S+RLEA+ FG+ DY+ R +IG Sbjct: 121 TQIEQAKKRDKRIGFEVLIETALGMANVEAIAQSSKRLEAMSFGVADYAASTRARSTVIG 180 Query: 181 ASDRRYAVLADAGEGGARRVHWNDQWHFALARIANACRAHGLRAIDGPYANFGDAAGFRA 240 + YAVL D G R+ HW D W FA R+ ACRA+GLR IDGP+ +F D GF+A Sbjct: 181 GVNPDYAVLTDKDAEGGRQTHWQDPWLFAQNRMLVACRAYGLRPIDGPFGDFSDPDGFKA 240 Query: 241 SAARAAALGFEGKWAIHPSQLETANEVFSPTAREIGWAREITAKIQSASAGGDGAFGQDG 300 +A R A LGFEGKWAIHPSQ+E ANEVF+PT E+ AR I ++ A+ G GA DG Sbjct: 241 AAKRCAVLGFEGKWAIHPSQIELANEVFTPTEAEVTKARRILQAMEEAARAGRGAVSLDG 300 Query: 301 VLIDLAVLKRAQAIIDRNDAIQ 322 LID+A ++ A+A+I++ DA++ Sbjct: 301 RLIDIASIRMAEALIEKADAMK 322