BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0008.p (387 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_772231.1| salicylate hydroxylase [Bradyrhizobium japonicu... 538 e-151 ref|YP_001204979.1| putative salicylate hydroxylase [Bradyrhizob... 530 e-148 ref|YP_001241132.1| putative salicylate 1-monooxygenase [Bradyrh... 514 e-144 ref|YP_533010.1| salicylate 1-monooxygenase [Rhodopseudomonas pa... 486 e-135 ref|YP_577826.1| monooxygenase, FAD-binding [Nitrobacter hamburg... 480 e-133 >ref|NP_772231.1| salicylate hydroxylase [Bradyrhizobium japonicum USDA 110] dbj|BAC50856.1| salicylate hydroxylase [Bradyrhizobium japonicum USDA 110] Length = 400 Score = 538 bits (1385), Expect = e-151, Method: Compositional matrix adjust. Identities = 285/399 (71%), Positives = 327/399 (81%), Gaps = 13/399 (3%) Query: 1 VALTGTVIVAGAGIGGLTASLTLAAQGFRVVVLEKAERLEEAGAGIQLAPNASRILVELG 60 +AL+ T+++AGAGIGGLTA+L LAA+GFR+VVLEKAERLEE GAG+QL+PNASR+LV LG Sbjct: 1 MALSRTIVIAGAGIGGLTAALALAARGFRIVVLEKAERLEEVGAGLQLSPNASRVLVGLG 60 Query: 61 LKEPLARRAVTPESVSILSARAGGEIARMPLGQAAEFRAGAPYWVIHRADLQAALQAAAS 120 L E L RAV PE+VSI+SARAGGE+ RMPLG+AA RAGAPYWV+HRADLQ+AL A S Sbjct: 61 LTERLKLRAVVPEAVSIMSARAGGELLRMPLGEAASVRAGAPYWVVHRADLQSALAGAVS 120 Query: 121 DHPDIDLRLGCQFEDVTKHARGLTVVQRRGNSRQEELAVALIGADGIWSSVRGHLFPDVQ 180 DHPDIDL+LG FEDV HA+GLTVV R G R+ +LA ALIGADGIWS+VR HLFP+VQ Sbjct: 121 DHPDIDLKLGATFEDVAPHAKGLTVVHRSGTIRRSDLASALIGADGIWSTVRQHLFPEVQ 180 Query: 181 PQFSGLIAWRGTLDATALPREYTAPRVQLWMGPDAHLVAYPISAGRQINVVAIVSGTWNR 240 P+FSGLIAWRGTLDAT LP++YTA RVQLWMGP+AHLVAYPI+ GRQINVVA++ GTWNR Sbjct: 181 PRFSGLIAWRGTLDATQLPKDYTARRVQLWMGPNAHLVAYPIAGGRQINVVAVLPGTWNR 240 Query: 241 PGWSEPGDINEIKAAFATARWPATARMLIGAVDGWRKWALFTLPDIGRWSEGAVTLLGDA 300 PGWS PGD E+ AFA RWP+ ARM++ AVDGWRKWALF +PD WS+G V LLGDA Sbjct: 241 PGWSTPGDPREVMDAFAAPRWPSQARMMLAAVDGWRKWALFGVPDGCPWSKGPVALLGDA 300 Query: 301 AHAMLPFAAQGAGMAIEDAAVLAKALSDSTGENTAGIPAALKRYARMRRGRVLKVQRLAR 360 HAMLPFAAQGAGMAIEDAAVLA+ LS T E+T GI AALK+Y R R+ RV +VQR AR Sbjct: 301 VHAMLPFAAQGAGMAIEDAAVLAQHLSPETAESTDGIVAALKQYGRARQARVRRVQRTAR 360 Query: 361 QQGR-------------XAIRAIGPERMLARQDWIYDWR 386 QQGR AIRA+GPERMLARQDWIY WR Sbjct: 361 QQGRIYHFSGPLAIARDVAIRALGPERMLARQDWIYGWR 399 >ref|YP_001204979.1| putative salicylate hydroxylase [Bradyrhizobium sp. ORS 278] emb|CAL76742.1| putative Salicylate hydroxylase (Salicylate 1-monooxygenase) [Bradyrhizobium sp. ORS 278] Length = 399 Score = 530 bits (1365), Expect = e-148, Method: Compositional matrix adjust. Identities = 279/394 (70%), Positives = 322/394 (81%), Gaps = 13/394 (3%) Query: 6 TVIVAGAGIGGLTASLTLAAQGFRVVVLEKAERLEEAGAGIQLAPNASRILVELGLKEPL 65 TV VAGAGIGGLTA+L LA +GFRVVVLEKAERLEE GAG+QL+PNASRIL++LGL L Sbjct: 5 TVTVAGAGIGGLTAALALATKGFRVVVLEKAERLEEVGAGLQLSPNASRILIDLGLGPRL 64 Query: 66 ARRAVTPESVSILSARAGGEIARMPLGQAAEFRAGAPYWVIHRADLQAALQAAASDHPDI 125 RAV PE+VSI+SARAGGEIAR+PLG A AGAPYWVIHRADLQAAL A A HPDI Sbjct: 65 TTRAVVPEAVSIMSARAGGEIARLPLGTTASESAGAPYWVIHRADLQAALAAEAMAHPDI 124 Query: 126 DLRLGCQFEDVTKHARGLTVVQRRGNSRQEELAVALIGADGIWSSVRGHLFPDVQPQFSG 185 +L+LGCQFEDV HA+GLTVV RRG+ R++++A+ALIGADG+WS+VR HLFP V+ FSG Sbjct: 125 ELKLGCQFEDVAAHAKGLTVVHRRGDERRQDVALALIGADGVWSAVRHHLFPQVRAAFSG 184 Query: 186 LIAWRGTLDATALPREYTAPRVQLWMGPDAHLVAYPISAGRQINVVAIVSGTWNRPGWSE 245 LIAWRGTL+A LPR+YT+ RVQLWMGP AHLVAYPISAGRQ+NVVA+V GTWNRPGWS Sbjct: 185 LIAWRGTLEARQLPRDYTSARVQLWMGPSAHLVAYPISAGRQVNVVAVVPGTWNRPGWST 244 Query: 246 PGDINEIKAAFATARWPATARMLIGAVDGWRKWALFTLPDIGRWSEGAVTLLGDAAHAML 305 GD E+KAAFA WPATAR+L+ AVDGWRKWALF +P+ WS+G V LLGDAAHAML Sbjct: 245 DGDPAELKAAFAPPGWPATARLLLNAVDGWRKWALFAVPEGIEWSKGNVALLGDAAHAML 304 Query: 306 PFAAQGAGMAIEDAAVLAKALSDSTGENTAGIPAALKRYARMRRGRVLKVQRLARQQGR- 364 PFAAQGAGMAIEDAAVLAKALS++ + AGI ALKRYA++RR RV +VQ AR+QGR Sbjct: 305 PFAAQGAGMAIEDAAVLAKALSEARPDGLAGIEGALKRYAKLRRPRVGQVQTTARKQGRI 364 Query: 365 ------------XAIRAIGPERMLARQDWIYDWR 386 +I+A+GPER+ ARQ WIYDWR Sbjct: 365 YHMSGPLALARDISIKALGPERLSARQRWIYDWR 398 >ref|YP_001241132.1| putative salicylate 1-monooxygenase [Bradyrhizobium sp. BTAi1] gb|ABQ37226.1| putative Salicylate 1-monooxygenase [Bradyrhizobium sp. BTAi1] Length = 399 Score = 514 bits (1324), Expect = e-144, Method: Compositional matrix adjust. Identities = 275/394 (69%), Positives = 320/394 (81%), Gaps = 13/394 (3%) Query: 6 TVIVAGAGIGGLTASLTLAAQGFRVVVLEKAERLEEAGAGIQLAPNASRILVELGLKEPL 65 T+ VAGAGIGGLTA+L LA +GFRVVVLEKAERLEE GAG+QL+PNASRIL++LGL L Sbjct: 5 TIFVAGAGIGGLTAALALATKGFRVVVLEKAERLEEVGAGLQLSPNASRILIDLGLGPRL 64 Query: 66 ARRAVTPESVSILSARAGGEIARMPLGQAAEFRAGAPYWVIHRADLQAALQAAASDHPDI 125 A R V PE+VSILSARAGGEIAR+PLG AA AGAPYWV+HRADLQAAL A A HPD+ Sbjct: 65 ASRVVVPEAVSILSARAGGEIARLPLGAAASEAAGAPYWVVHRADLQAALAAEAMAHPDV 124 Query: 126 DLRLGCQFEDVTKHARGLTVVQRRGNSRQEELAVALIGADGIWSSVRGHLFPDVQPQFSG 185 +LRLGCQFEDV HA+GLTVV RRG+ R++++A+ALIGADG+WS+VR HLFP V+ +FSG Sbjct: 125 ELRLGCQFEDVAAHAKGLTVVHRRGDERRQDVALALIGADGVWSAVRHHLFPQVRAEFSG 184 Query: 186 LIAWRGTLDATALPREYTAPRVQLWMGPDAHLVAYPISAGRQINVVAIVSGTWNRPGWSE 245 LIAWRGTLDA LPR++ + RVQLWMGP AHLVAYPISAGRQ+NVVA+V GTWNRPGWS Sbjct: 185 LIAWRGTLDARQLPRDHASARVQLWMGPGAHLVAYPISAGRQVNVVAVVPGTWNRPGWSA 244 Query: 246 PGDINEIKAAFATARWPATARMLIGAVDGWRKWALFTLPDIGRWSEGAVTLLGDAAHAML 305 GD E+KAAF RWPATAR+L+ AVDGWRKWALF +P+ W+ G V LLGDAAHAML Sbjct: 245 EGDPAELKAAFGPPRWPATARLLLNAVDGWRKWALFGVPEGIAWTAGNVALLGDAAHAML 304 Query: 306 PFAAQGAGMAIEDAAVLAKALSDSTGENTAGIPAALKRYARMRRGRVLKVQRLARQQGRX 365 PFAAQGAGMAIEDAAVLAK LS++ + I AL+RYAR+RR RV +VQR ARQQGR Sbjct: 305 PFAAQGAGMAIEDAAVLAKTLSEARPDGPGAIEGALQRYARLRRPRVGRVQRTARQQGRV 364 Query: 366 -------------AIRAIGPERMLARQDWIYDWR 386 +I+A+GP+R+ ARQ WIYDWR Sbjct: 365 YHMTGPLALARDLSIKALGPQRLSARQSWIYDWR 398 >ref|YP_533010.1| salicylate 1-monooxygenase [Rhodopseudomonas palustris BisB18] gb|ABD88691.1| monooxygenase, FAD-binding [Rhodopseudomonas palustris BisB18] Length = 398 Score = 486 bits (1251), Expect = e-135, Method: Compositional matrix adjust. Identities = 250/394 (63%), Positives = 301/394 (76%), Gaps = 15/394 (3%) Query: 6 TVIVAGAGIGGLTASLTLAAQGFRVVVLEKAERLEEAGAGIQLAPNASRILVELGLKEPL 65 T++VAGAGIGGLTASLTLAA+GFRV+VLEKA+RLEEAGAG+QL+PNASR+L++LGL+ L Sbjct: 6 TIVVAGAGIGGLTASLTLAAKGFRVIVLEKAQRLEEAGAGLQLSPNASRVLIDLGLRPRL 65 Query: 66 ARRAVTPESVSILSARAGGEIARMPLGQAAEFRAGAPYWVIHRADLQAALQAAASDHPDI 125 +TP++V+I+SAR GEI R+PLG A FRAGAPYWVIHRADLQ AL A DH +I Sbjct: 66 EPSVITPDAVTIMSARGQGEIVRLPLGDEARFRAGAPYWVIHRADLQTALAAQVRDHRNI 125 Query: 126 DLRLGCQFEDVTKHARGLTVVQRRGNSRQEELAVALIGADGIWSSVRGHLFPDVQPQFSG 185 +LRLG QFE+V+ A G++V QR G SR E A+AL+GADGIWS+VR LFPD QP+FSG Sbjct: 126 ELRLGWQFEEVSSTADGVSVTQRNGLSRLHEPALALVGADGIWSAVRRQLFPDAQPKFSG 185 Query: 186 LIAWRGTLDATALPREYTAPRVQLWMGPDAHLVAYPISAGRQINVVAIVSGTWNRPGWSE 245 LIAWRGT +A LP + A VQLWMG +AHL+AYPISAGR+IN+VAIV+G+WNRPGWS Sbjct: 186 LIAWRGTFEADRLPAGFAARNVQLWMGGNAHLIAYPISAGRRINIVAIVAGSWNRPGWSA 245 Query: 246 PGDINEIKAAFATARWPATARMLIGAVDGWRKWALFTLPDIGRWSEGAVTLLGDAAHAML 305 PGD EI + FA WP AR+LI AV GWR+WALFT+ D G W+ GA +LGDAAH ML Sbjct: 246 PGDPGEINSQFAPPHWPDQARVLIDAVQGWRRWALFTMQDGGVWNHGAAAMLGDAAHGML 305 Query: 306 PFAAQGAGMAIEDAAVLAKALSDSTGENTAGIPAALKRYARMRRGRVLKVQRLARQQGR- 364 PFAAQGAGMAIEDAAVLA L D + +PAAL+RYA +R+ RV +VQR AR G+ Sbjct: 306 PFAAQGAGMAIEDAAVLAACLGDISAPEA--VPAALQRYAELRQPRVGRVQRTARLNGQI 363 Query: 365 ------------XAIRAIGPERMLARQDWIYDWR 386 +RA+G R+LARQ WIYDWR Sbjct: 364 YHLAGAAALARDLTMRALGGPRLLARQRWIYDWR 397 >ref|YP_577826.1| monooxygenase, FAD-binding [Nitrobacter hamburgensis X14] gb|ABE63366.1| monooxygenase, FAD-binding protein [Nitrobacter hamburgensis X14] Length = 400 Score = 480 bits (1236), Expect = e-133, Method: Compositional matrix adjust. Identities = 247/394 (62%), Positives = 299/394 (75%), Gaps = 13/394 (3%) Query: 6 TVIVAGAGIGGLTASLTLAAQGFRVVVLEKAERLEEAGAGIQLAPNASRILVELGLKEPL 65 TV+VAGAGIGGLTASL LAA+GFR++++E AERLEEAGAG+QL+PNASRIL+ LGL L Sbjct: 6 TVVVAGAGIGGLTASLALAAKGFRILIIETAERLEEAGAGLQLSPNASRILIGLGLHARL 65 Query: 66 ARRAVTPESVSILSARAGGEIARMPLGQAAEFRAGAPYWVIHRADLQAALQAAASDHPDI 125 + RA+ PE++SI+SAR GGEI R+PLG+AA RAGAPYWV+HRA LQAAL A DHP+I Sbjct: 66 SARALVPEAISIMSARHGGEICRLPLGEAAALRAGAPYWVVHRAGLQAALLEQARDHPEI 125 Query: 126 DLRLGCQFEDVTKHARGLTVVQRRGNSRQEELAVALIGADGIWSSVRGHLFPDVQPQFSG 185 +L LG E V H G+T+ R G + ++E A ALIGADG+WS++R LFPD +P FSG Sbjct: 126 ELCLGAAIESVAAHGNGVTLGVRHGMTSRQETAWALIGADGVWSALRTRLFPDARPLFSG 185 Query: 186 LIAWRGTLDATALPREYTAPRVQLWMGPDAHLVAYPISAGRQINVVAIVSGTWNRPGWSE 245 L AWRGT++A LP + TA RV+LWMGPDAHLVAYP+S GRQIN+VA+V WN+PGWS Sbjct: 186 LTAWRGTIEAAQLPHDMTARRVRLWMGPDAHLVAYPMSGGRQINIVAVVPDAWNQPGWSA 245 Query: 246 PGDINEIKAAFATARWPATARMLIGAVDGWRKWALFTLPDIGRWSEGAVTLLGDAAHAML 305 GD EIK F+ ARW ARM+I AVDGWRKWALFT+P + W A+ LLGDAAHAML Sbjct: 246 AGDAAEIKHRFSQARWSEPARMMIDAVDGWRKWALFTIPPMAAWHRDAMALLGDAAHAML 305 Query: 306 PFAAQGAGMAIEDAAVLAKALSDSTGENTAGIPAALKRYARMRRGRVLKVQRLARQQGR- 364 PFAAQGAGMAIEDAAVLA+ L ++ E+ G AAL RY +RR RV +VQ LA++ GR Sbjct: 306 PFAAQGAGMAIEDAAVLAQCLGEAANESAHGAAAALARYTSLRRARVARVQALAQRNGRI 365 Query: 365 ------------XAIRAIGPERMLARQDWIYDWR 386 AIRA+GP R+LARQDWIY+WR Sbjct: 366 YHLGGAAALARDLAIRAMGPRRLLARQDWIYNWR 399