BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0005.p (404 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_768633.1| polygalacturonase [Bradyrhizobium japonicum USD... 662 0.0 ref|YP_001691269.1| hypothetical protein M446_7020 [Methylobacte... 458 e-127 ref|YP_003519390.1| Peh [Pantoea ananatis LMG 20103] >gi|2911516... 396 e-108 ref|YP_002755698.1| endo-polygalacturonase/pectinesterase [Acido... 330 3e-88 ref|YP_001907325.1| Endo-polygalacturonase [Erwinia tasmaniensis... 322 8e-86 >ref|NP_768633.1| polygalacturonase [Bradyrhizobium japonicum USDA 110] gb|AAG60962.1|AF322013_81 ID636 [Bradyrhizobium japonicum] dbj|BAC47258.1| blr1993 [Bradyrhizobium japonicum USDA 110] Length = 510 Score = 662 bits (1709), Expect = 0.0, Method: Compositional matrix adjust. Identities = 338/400 (84%), Positives = 360/400 (90%) Query: 1 MTKHFREWTLMSIVATMVVCPSAKAQDRRIVAEPVAPHTVCDRPVPSGSNDTARLQDAID 60 MTK FR W L+SI+A+MVV P+AKAQDRR +AEPVAP TVCDRPVPSG NDT LQ+AI+ Sbjct: 79 MTKRFRNWALLSILASMVVGPAAKAQDRRTIAEPVAPDTVCDRPVPSGKNDTVSLQEAIE 138 Query: 61 HCPAGAAVYLGRGEFQSHPLEMKSGVTLWIGRGATLLAIPEPVAYDKGRGQCGRIAGKGD 120 HCP GAAVYLGRGEF S PLEMKSGVTLW+GRGATL+AIPEP AYDKG GQCGRIA KGD Sbjct: 139 HCPDGAAVYLGRGEFHSGPLEMKSGVTLWVGRGATLIAIPEPAAYDKGLGQCGRIAEKGD 198 Query: 121 GCRPFISFSKTRGGGIYGDGIIDGQGGAPMVGSAETWWQLARRAQAEGGSQNAPRLIQID 180 GCRPFISFSKTRGGGIYG+GIIDGQGGA + G AETWWQLARRAQA+GG+QNAPRLIQ+D Sbjct: 199 GCRPFISFSKTRGGGIYGEGIIDGQGGALIGGGAETWWQLARRAQAQGGNQNAPRLIQVD 258 Query: 181 HAQDITLSGVTLRNAPNFHVAMNRVEGATVWGLTIDTPADARNTDGIDPGASQDVTIIHS 240 HAQDIT SGVTLRNA NFHVAMNRVEGAT W LTID+PADARNTDGID GASQDVTI HS Sbjct: 259 HAQDITFSGVTLRNAANFHVAMNRVEGATFWALTIDSPADARNTDGIDLGASQDVTITHS 318 Query: 241 FIRTGDDNVAIKAGDNGSTRHISITDNYFGWGHGMSIGSEVNSGASDILVSNLTLDGTTS 300 +IRTGDDNVAIKAGDNGS+ HISI DNYFGWGHGMSIGSEVNSGA DILV NLTLDGTTS Sbjct: 319 WIRTGDDNVAIKAGDNGSSSHISIIDNYFGWGHGMSIGSEVNSGARDILVRNLTLDGTTS 378 Query: 301 GLRIKSDVSRGGLVERVTYENVCLRGNRWPVAFDTKYDPHAQGSRIPVYRQIVLRHVRGD 360 GLRIKSDV RGGLVE VTYE VCLRGNRWP+AFD KYDP AQG+RIPVYRQIVLRHV GD Sbjct: 379 GLRIKSDVGRGGLVENVTYEYVCLRGNRWPLAFDAKYDPRAQGTRIPVYRQIVLRHVHGD 438 Query: 361 NGALLMRGVDEGHALDVTLEDVRFADSATWQLEHANXECD 400 G LLMRG+DE HALDVTLEDVRFA+SATWQLEHAN + Sbjct: 439 TGVLLMRGIDERHALDVTLEDVRFANSATWQLEHANVSAN 478 >ref|YP_001691269.1| hypothetical protein M446_7020 [Methylobacterium sp. 4-46] gb|ACA21244.1| hypothetical protein M446_7020 [Methylobacterium sp. 4-46] Length = 449 Score = 458 bits (1178), Expect = e-127, Method: Compositional matrix adjust. Identities = 231/391 (59%), Positives = 282/391 (72%), Gaps = 12/391 (3%) Query: 10 LMSIVATMVVCPSAKAQDRRIVAEPVAPHTVCD----RPVPSGSNDTARLQDAIDHCPAG 65 LM++ AT SA++QDRR V EPV P VC PS A+LQ A+D C G Sbjct: 16 LMTVGATH----SAQSQDRRNVQEPVPPTQVCAVLSADDAPSAKVGQAKLQAALDGCRTG 71 Query: 66 AAVYLG----RGEFQSHPLEMKSGVTLWIGRGATLLAIPEPVAYDKGRGQCGRIAGKGDG 121 AV L + F S P+EM++GV LW+ GA L A P+P YD G G+CG I G G Sbjct: 72 QAVRLTPSRTKAVFASGPIEMRAGVVLWLDAGAVLAAEPDPRLYDLGDGRCGLINNAGGG 131 Query: 122 CRPFISFSKTRGGGIYGDGIIDGQGGAPMVGSAETWWQLARRAQAEGGSQNAPRLIQIDH 181 CRPF++F K G G+ GDG IDGQGGA M+G AETWW LA RAQ EG QN PRL+QID Sbjct: 132 CRPFLNFHKANGAGLMGDGTIDGQGGAVMIGKAETWWALASRAQVEGREQNVPRLVQIDS 191 Query: 182 AQDITLSGVTLRNAPNFHVAMNRVEGATVWGLTIDTPADARNTDGIDPGASQDVTIIHSF 241 + +IT+ +TLRNAPN H+AMN+V+G TVWG+TI+TPADARNTDGIDPGA+ DVTI +F Sbjct: 192 SNNITVYRITLRNAPNVHIAMNKVQGVTVWGVTINTPADARNTDGIDPGAAMDVTIARTF 251 Query: 242 IRTGDDNVAIKAGDNGSTRHISITDNYFGWGHGMSIGSEVNSGASDILVSNLTLDGTTSG 301 I TGDD++AIKAGDNG+TRH+SI D + GHG+SIGSE NSG SD+LV N+T+DG SG Sbjct: 252 ISTGDDDIAIKAGDNGATRHVSILDIHIYSGHGLSIGSETNSGVSDVLVRNVTIDGAVSG 311 Query: 302 LRIKSDVSRGGLVERVTYENVCLRGNRWPVAFDTKYDPHAQGSRIPVYRQIVLRHVRGDN 361 LRIKSD SRGGLV+ V YENVC+RG+R P+ FDT+YDP AQG++IPVY IVLR V G+ Sbjct: 312 LRIKSDPSRGGLVQAVRYENVCVRGSRRPIDFDTRYDPRAQGTKIPVYSNIVLRQVAGEG 371 Query: 362 GALLMRGVDEGHALDVTLEDVRFADSATWQL 392 G L+M G D H L V+L+ VRF D+ATW + Sbjct: 372 GRLVMHGYDAAHPLGVSLDGVRFVDNATWDV 402 >ref|YP_003519390.1| Peh [Pantoea ananatis LMG 20103] gb|ADD76262.1| Peh [Pantoea ananatis LMG 20103] Length = 417 Score = 396 bits (1017), Expect = e-108, Method: Compositional matrix adjust. Identities = 205/395 (51%), Positives = 264/395 (66%), Gaps = 7/395 (1%) Query: 6 REWTLMSIVATMVVCPSAKAQDRRIVAEPVAPHTVCDRPVPSG-SNDTARLQDAIDHCPA 64 R +++ V ++++ +A+A DRR V +PV+P VC R S SN +Q A+++CP Sbjct: 10 RSASILFAVLSVLMSFTAQAGDRRRVTQPVSP-PVCQRIYASNQSNIGPHIQQALNNCPP 68 Query: 65 GAAVYL----GRGEFQSHPLEMKSGVTLWIGRGATLLAIPEPVAYDKGRGQCGRIAGKGD 120 G V L S PL + SGV+L + +GA L AIP+P YD GRG CG++ +G+ Sbjct: 69 GRMVLLVPSPTVKTLTSGPLSLPSGVSLGVDKGAVLSAIPDPRLYDNGRGSCGKLDNQGN 128 Query: 121 GCRPFISFSKTRGGGIYGDGIIDGQGGAPMVGSAETWWQLARRAQAEGGSQNAPRLIQID 180 GCR FI+ + G GIYG G+IDGQG A M G +TWWQLA A+ + QNAPRLIQID Sbjct: 129 GCRAFITLTHGHGSGIYGQGVIDGQGAASMAGEGQTWWQLAASARNDEAKQNAPRLIQID 188 Query: 181 HAQDITLSGVTLRNAPNFHVAMNRVEGATVWGLTIDTPADARNTDGIDPGASQDVTIIHS 240 HA DITL +TL+NAPNFHV +N +G T+WG+TIDTPA ARNTDG+DP S DVT+I+S Sbjct: 189 HASDITLYQITLKNAPNFHVVINDSQGITLWGITIDTPATARNTDGVDPMGSSDVTLINS 248 Query: 241 FIRTGDDNVAIKAGDNGSTRHISITDNYFGWGHGMSIGSEVNSGASDILVSNLTLDGTTS 300 I TGDDNVAIKAG N HISI +N FG+GHGMSIGSE+N G SD+LV LTL GTT+ Sbjct: 249 RISTGDDNVAIKAG-NAPASHISILNNQFGFGHGMSIGSEINRGVSDVLVDGLTLTGTTN 307 Query: 301 GLRIKSDVSRGGLVERVTYENVCLRGNRWPVAFDTKYDPHAQGSRIPVYRQIVLRHVRGD 360 GLRIKSD SRGG V + Y+NVC++ PV DT YD HA G+ IP YR I +V+ Sbjct: 308 GLRIKSDRSRGGQVRAIRYQNVCMQNVDNPVVMDTHYDTHASGTMIPDYRDITFDNVKTG 367 Query: 361 NGALLMRGVDEGHALDVTLEDVRFADSATWQLEHA 395 G L + G E + L++TL++V + AT + A Sbjct: 368 KGKLTLDGYSEVYPLEMTLKNVTVGEQATIEERFA 402 >ref|YP_002755698.1| endo-polygalacturonase/pectinesterase [Acidobacterium capsulatum ATCC 51196] gb|ACO32486.1| endo-polygalacturonase/pectinesterase [Acidobacterium capsulatum ATCC 51196] Length = 794 Score = 330 bits (845), Expect = 3e-88, Method: Compositional matrix adjust. Identities = 180/396 (45%), Positives = 243/396 (61%), Gaps = 19/396 (4%) Query: 5 FREWTLMSIVATMVVCPSA-KAQDRRIVAEPVAPHT----VCDRPVPSGS--------ND 51 R ++ + A + +CP+ AQD R V EP P P G D Sbjct: 6 LRRASVALVSALIALCPAMIHAQDTRHVVEPHFPAACVTLTAHLSAPGGKLTAFEEQQED 65 Query: 52 TARLQDAIDHCPAGAAVYL-GRGE---FQSHPLEMKSGVTLWIGRGATLLAIPEPVAYDK 107 T R+Q AI+HC G AV L GE F S PL+++ G+TL + G L A P YD Sbjct: 66 TTRIQQAINHCTLGHAVELRSSGEDDIFLSGPLQLRRGITLRVDSGTALYASRNPRDYDL 125 Query: 108 GRGQCGRIAGKGDGCRPFISFSKTRGGGIYGDGIIDGQGGAPMVGSAETWWQLARRAQAE 167 G CG + +G GC+P I+ G GI G+G+IDG+GGA ++G TWW LA +A+ Sbjct: 126 TPGSCGVVNQRGHGCKPLITADHAPGSGIMGNGVIDGRGGATLLGQKVTWWDLAHQAKIR 185 Query: 168 GGSQNAPRLIQIDHAQDITLSGVTLRNAPNFHVAMNRVEGATVWGLTIDTPADARNTDGI 227 +Q+ PR++ I+ + + L +TLRN+PNFHV +N G T WG+ IDTPA ARNTDGI Sbjct: 186 DLNQSCPRILVINRSNNFILYRITLRNSPNFHVLLNHTNGFTAWGVRIDTPATARNTDGI 245 Query: 228 DPGASQDVTIIHSFIRTGDDNVAIKAGDNGSTRHISITDNYFGWGHGMSIGSEVNSGASD 287 DP AS++VTI HS+IR GDD+VAIKAG GS+ H++I+D++F GHGMSIGSE N G D Sbjct: 246 DPAASENVTITHSYIRDGDDDVAIKAGTGGSSSHMTISDDHFYSGHGMSIGSETNGGVRD 305 Query: 288 ILVSNLTLDGTTSGLRIKSDVSRGGLVERVTYENVCLRGNRWPVAFDTKYDPHAQGSRIP 347 ILV NL+LDGTT+G+RIKSD+SRGGLV+ VTY N+C+R P+ Y G+ +P Sbjct: 306 ILVENLSLDGTTNGIRIKSDLSRGGLVDHVTYRNICMRDVPHPILLTPHY-ARKHGNLVP 364 Query: 348 VYRQIVLRHVR-GDNGALLMRGVDEGHALDVTLEDV 382 YR I+L++V +G + G D H+L V ++V Sbjct: 365 QYRDILLQNVHILTHGDYTLLGEDAVHSLAVQFDNV 400 >ref|YP_001907325.1| Endo-polygalacturonase [Erwinia tasmaniensis Et1/99] emb|CAO96432.1| Endo-polygalacturonase [Erwinia tasmaniensis Et1/99] Length = 406 Score = 322 bits (825), Expect = 8e-86, Method: Compositional matrix adjust. Identities = 180/399 (45%), Positives = 246/399 (61%), Gaps = 15/399 (3%) Query: 4 HFREWTLMSIVATMVVCPSAKAQDRRIVAEPVAPHTVCDRPVPSGSN-DTARLQDAIDHC 62 + + +L+ I +T + ++A+D R V+EP P VC +P+G+ T +Q A++ C Sbjct: 12 NLTKISLLIIFSTGI----SEAKDLRNVSEPKYP-AVC-SVLPAGAAVSTDAIQKALNAC 65 Query: 63 PAGAAVYL----GRGEFQSHPLEMKSGVTLWIGRGATLLAIPEPVAYDKGRGQCGRIAGK 118 P G AV L G F S PL + SG +LW+ G TL A+ A+DK + CG + Sbjct: 66 PQGQAVKLQKSAGGAVFLSGPLSLPSGRSLWVDGGVTLKAVNSAAAFDKVKNSCGVLDKS 125 Query: 119 GDGCRPFISFSKTRGGGIYGDGIIDGQGGAPMVGSAETWWQLARRAQAEGGSQNAPRLIQ 178 G GC FI+ S R GIYG GIIDGQGG + TWWQLA +A+ + QNAPRLIQ Sbjct: 126 GKGCEAFITVSNARNSGIYGKGIIDGQGGVTLQDKKMTWWQLAAQAKEKSMKQNAPRLIQ 185 Query: 179 IDHAQDITLSGVTLRNAPNFHVAMNRVEGATVWGLTIDTPADARNTDGIDPGASQDVTII 238 ID++ D TL +TL N+PNFHV N+ G TVW TI+TP DARNTDGIDP +S++VTI Sbjct: 186 IDNSTDFTLYDITLTNSPNFHVVFNKSNGLTVWNTTINTPRDARNTDGIDPISSKNVTIA 245 Query: 239 HSFIRTGDDNVAIKA-GDNGSTRHISITDNYFGWGHGMSIGSEVNSGASDILVSNLTLDG 297 HS I TGDDNVAIKA D GS+++IS+ N FG+GHGMSIGSE N G D+LV +LTL G Sbjct: 246 HSNISTGDDNVAIKAYSDKGSSQNISVIHNTFGFGHGMSIGSETN-GIYDVLVDDLTLSG 304 Query: 298 TTSGLRIKSDVSRGGLVERVTYENVCLRGNRWPVAFDTKYDPHAQGSRIPVYRQIVLRHV 357 T +GLRIKSD S G V+ VTY+NV + + P+ DT Y+ A GS+ ++ I + + Sbjct: 305 TDNGLRIKSDKSNAGEVDGVTYKNVTMTNVKRPIVIDTVYENKA-GSKKADWKNISYQDI 363 Query: 358 RGDN-GALLMRGVDEGHALDVTLEDVRFADSATWQLEHA 395 G + + G + + V + +V+ + + L +A Sbjct: 364 TSKGAGVVNLNGQNAVRKIVVKMSNVKLDSATKYTLNNA 402