BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0004.p (330 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_768634.1| pectinesterase [Bradyrhizobium japonicum USDA 1... 542 e-152 ref|YP_004087203.1| pectinesterase [Asticcacaulis excentricus CB... 295 8e-78 ref|ZP_08265027.1| pectinesterase family protein [Asticcacaulis ... 290 3e-76 ref|YP_002755698.1| endo-polygalacturonase/pectinesterase [Acido... 286 2e-75 ref|YP_004217013.1| pectinesterase [Acidobacterium sp. MP5ACTX9]... 275 7e-72 >ref|NP_768634.1| pectinesterase [Bradyrhizobium japonicum USDA 110] gb|AAG60963.1|AF322013_82 ID637 [Bradyrhizobium japonicum] dbj|BAC47259.1| blr1994 [Bradyrhizobium japonicum USDA 110] Length = 346 Score = 542 bits (1396), Expect = e-152, Method: Compositional matrix adjust. Identities = 256/322 (79%), Positives = 286/322 (88%) Query: 1 MRIFALFAAVLAGCFGISAARAQPLLVSHAERAAYHSLQAAIDALPAQGGDIQIAPGIYR 60 MRIF AA+LAGCF I+AA A PLLVSHA A YHS+Q AIDALPA+GG+++IAPGIYR Sbjct: 1 MRIFVSSAALLAGCFCIAAAYAHPLLVSHAGNADYHSVQQAIDALPAEGGNVRIAPGIYR 60 Query: 61 EKVKVFKSGVHIKGSGRMPGDTVIVYGDGAINVGGTIRSATLDASGDDFRLDNLTIQNDY 120 EKVK+ KSGVH+ G+G P DTVIVYGDGA+NVGGT RSATLDA DDFRL+NLTIQNDY Sbjct: 61 EKVKITKSGVHLAGTGNKPEDTVIVYGDGAVNVGGTARSATLDAPSDDFRLENLTIQNDY 120 Query: 121 ALNSANPASQAVALSVTGDRDIISRVRLLGAQDTLFAGKGQNGRMSRQYFSDCYIEGHVD 180 ALN ANP SQAVALSVTGDRD+I+RVRLLGAQDTLFAGKG NGRMSRQYFS+CYIEGHVD Sbjct: 121 ALNPANPPSQAVALSVTGDRDVITRVRLLGAQDTLFAGKGPNGRMSRQYFSNCYIEGHVD 180 Query: 181 FIFGNAKAYFRHCELHGIANQAVVYTAQSKAAPDEDSAYVFEHCILTADSAARDVALGRP 240 FIFGNAK +FR CELHGIANQAV+YTAQSKA PDEDS YVF+HC L+AD AAR +ALGRP Sbjct: 181 FIFGNAKVWFRQCELHGIANQAVIYTAQSKATPDEDSGYVFDHCRLSADPAARYIALGRP 240 Query: 241 WRPYATVVFLSTKLDAPVVAEGWREWAPGKTNSLRTAYYAEYKSTGVGANPAGREPYSHQ 300 WR YATVVFLSTK+DA V+AEGWREWAPG+TN L T+YYAEY+S G+GANP+GREP+SHQ Sbjct: 241 WRSYATVVFLSTKIDARVIAEGWREWAPGETNKLSTSYYAEYRSFGIGANPSGREPHSHQ 300 Query: 301 LTDGEAAKWSLKAFFAGATDWL 322 L DGEAA+WSL+ FFAG TDWL Sbjct: 301 LKDGEAARWSLRVFFAGVTDWL 322 >ref|YP_004087203.1| pectinesterase [Asticcacaulis excentricus CB 48] gb|ADU13052.1| Pectinesterase [Asticcacaulis excentricus CB 48] Length = 327 Score = 295 bits (754), Expect = 8e-78, Method: Compositional matrix adjust. Identities = 149/282 (52%), Positives = 188/282 (66%), Gaps = 3/282 (1%) Query: 35 YHSLQAAIDALPAQGGDIQIAPGIYREKVKVFKSGVHIKGSGRMPGDTVIVYGDGAINVG 94 Y +L A ALP GG ++IAPG YREK+ + K GV + G G+ P D VIV+GD A G Sbjct: 41 YKTLAEAFAALPEAGGVVEIAPGTYREKLSLSKPGVQLIGKGKKPEDVVIVWGDSAKMAG 100 Query: 95 GTIRSATLDASGDDFRLDNLTIQNDYALNSANPASQAVALSVTGDRDIISRVRLLGAQDT 154 GT +SA+ SGD FR NLTIQNDY L + SQAVALS++ DR ++ VRLLGAQDT Sbjct: 101 GTGKSASFTVSGDGFRASNLTIQNDYHLTQPDNPSQAVALSISADRAVLRNVRLLGAQDT 160 Query: 155 LFAGKGQNGRMSRQYFSDCYIEGHVDFIFGNAKAYFRHCELHGIANQAVVYTAQSKAAPD 214 L+AG + SRQY+ DCYIEGHVDFIFGNA A+F C LH I TA S+ A Sbjct: 161 LYAGSKKPTVPSRQYYKDCYIEGHVDFIFGNALAFFDRCHLHIIKRDGAFITAHSRTADS 220 Query: 215 EDSAYVFEHC-ILTADSAARDVALGRPWRPYATVVFLSTKLDAPVVAEGWREWAPGKTNS 273 E +AYVF+HC I TA + A GR WRPYA V+FL T++D + EGWREW PGKT + Sbjct: 221 ETTAYVFDHCRITTAGTGA--YYFGRAWRPYAQVIFLDTRIDGQIHPEGWREWTPGKTET 278 Query: 274 LRTAYYAEYKSTGVGANPAGREPYSHQLTDGEAAKWSLKAFF 315 TA++AEY S+G GA+ + R ++ +LT +AAKW L++ F Sbjct: 279 YGTAHFAEYNSSGPGADVSQRVFWAKRLTADQAAKWRLESVF 320 >ref|ZP_08265027.1| pectinesterase family protein [Asticcacaulis biprosthecum C19] gb|EGF91662.1| pectinesterase family protein [Asticcacaulis biprosthecum C19] Length = 323 Score = 290 bits (741), Expect = 3e-76, Method: Compositional matrix adjust. Identities = 146/283 (51%), Positives = 190/283 (67%), Gaps = 2/283 (0%) Query: 33 AAYHSLQAAIDALPAQGGDIQIAPGIYREKVKVFKSGVHIKGSGRMPGDTVIVYGDGAIN 92 A Y ++ A++ALP GG ++I+PG YREK + K+ VH+ G+G P D V+V+GD A Sbjct: 33 ADYKTVIEALNALPETGGTVEISPGTYREKWALSKTDVHLIGTGAKPEDVVLVWGDSAKM 92 Query: 93 VGGTIRSATLDASGDDFRLDNLTIQNDYALNSANPASQAVALSVTGDRDIISRVRLLGAQ 152 GGT +SA+L +GD FR N+TIQNDY LN + P SQAVAL +T DR ++ VRLLGAQ Sbjct: 93 AGGTGKSASLTITGDGFRASNVTIQNDYHLNRSEP-SQAVALYLTSDRAVLQNVRLLGAQ 151 Query: 153 DTLFAGKGQNGRMSRQYFSDCYIEGHVDFIFGNAKAYFRHCELHGIANQAVVYTAQSKAA 212 DTL+A + SRQY+ DCYIEGHVDFIFGNA A+F +H IA + TA S+ A Sbjct: 152 DTLYAASKKPTEPSRQYYRDCYIEGHVDFIFGNALAFFDRFVIHIIAREQAFITAHSRTA 211 Query: 213 PDEDSAYVFEHCILTADSAARDVALGRPWRPYATVVFLSTKLDAPVVAEGWREWAPGKTN 272 E +AY+F+HC +T + GR WRPY VVFL T++D + EGWREW PGKT Sbjct: 212 ETETTAYIFDHCTVTT-AGDGSYYFGRAWRPYGQVVFLDTRIDGKIHPEGWREWTPGKTE 270 Query: 273 SLRTAYYAEYKSTGVGANPAGREPYSHQLTDGEAAKWSLKAFF 315 + TA++AEY STG GAN A R ++ +LT +AAKW L+A F Sbjct: 271 TYTTAHFAEYGSTGPGANDAMRVFWADRLTPEDAAKWRLEAVF 313 >ref|YP_002755698.1| endo-polygalacturonase/pectinesterase [Acidobacterium capsulatum ATCC 51196] gb|ACO32486.1| endo-polygalacturonase/pectinesterase [Acidobacterium capsulatum ATCC 51196] Length = 794 Score = 286 bits (733), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 150/299 (50%), Positives = 193/299 (64%), Gaps = 8/299 (2%) Query: 25 LLVSHAERAAYHSLQAAIDALPAQGGDIQIAPGIYREKVKVFKSGVHIKGSGRMPGDTVI 84 L VS YH++Q+AIDA PA G I IAPG YRE V + K +H+ G G P TVI Sbjct: 476 LYVSKDSTGEYHTVQSAIDAAPATGAIIHIAPGTYREAVVIDKPNIHLIGGGPDPSSTVI 535 Query: 85 VYGDGAINVGGTIRSATLDASGDDFRLDNLTIQNDY--ALNSANPASQAVALSVTGDRDI 142 V A GGT++SAT+ G+ F NLTI ND+ + SQAVAL++T D+ I Sbjct: 536 VDDKSAGTSGGTLQSATVTVRGNGFFAANLTIANDWNRTHTQVSQGSQAVALAITADKAI 595 Query: 143 ISRVRLLGAQDTLFAGKGQNG------RMSRQYFSDCYIEGHVDFIFGNAKAYFRHCELH 196 ++ VRLLG QDTL+AG + +RQ FS C I G+VDFIFGN+KAYF++C L Sbjct: 596 LTHVRLLGNQDTLYAGSRKCNAAHTACTTARQLFSHCTIAGNVDFIFGNSKAYFQNCTLI 655 Query: 197 GIANQAVVYTAQSKAAPDEDSAYVFEHCILTADSAARDVALGRPWRPYATVVFLSTKLDA 256 + + TAQSK AP +DSA+VF+HC L A+ +V LGRPWRPYATV+FL T + Sbjct: 656 STPHSEGMITAQSKDAPQQDSAFVFDHCRLLAEPGVTNVWLGRPWRPYATVIFLHTFMGP 715 Query: 257 PVVAEGWREWAPGKTNSLRTAYYAEYKSTGVGANPAGREPYSHQLTDGEAAKWSLKAFF 315 + A GWREW PG T+SL TA++AE+ STG GA PA REPYSHQLT + +++L F+ Sbjct: 716 QIAAAGWREWHPGVTHSLATAWFAEFHSTGPGAYPAAREPYSHQLTASQQNRFTLAHFY 774 >ref|YP_004217013.1| pectinesterase [Acidobacterium sp. MP5ACTX9] gb|ADW68233.1| Pectinesterase [Acidobacterium sp. MP5ACTX9] Length = 332 Score = 275 bits (703), Expect = 7e-72, Method: Compositional matrix adjust. Identities = 147/299 (49%), Positives = 189/299 (63%), Gaps = 7/299 (2%) Query: 32 RAAYHSLQAAIDALPAQGGDIQIAPGIYREKVKVFKSGVHIKGSGRMPGDTVIVYGDGAI 91 RA + ++Q A+ A P G I+I PGIYRE V V K+ + ++G + VIV G Sbjct: 32 RADFATVQEAVTAAPETGAVIRIRPGIYREVVHVDKANIQMRGETKDASTVVIVDDMGDP 91 Query: 92 NVGGTIRSATLDASGDDFRLDNLTIQNDYALNSANPASQAVALSVTGDRDIISRVRLLGA 151 GT S T+ +GD F NLTI ND + + P +Q VALS+TGDR ++ VRLLGA Sbjct: 92 KTCGTFCSPTMFVTGDGFVASNLTISNDLS-KTGKPRTQGVALSITGDRAVLRNVRLLGA 150 Query: 152 QDTLFAGK-----GQNGRMSRQYFSDCYIEGHVDFIFGNAKAYFRHCELHGIANQAVVY- 205 QDTL+A G + SRQY+ CYIEG VDFIFGNAKA F CE+H + ++A Y Sbjct: 151 QDTLYAASRKCAAGAECKASRQYYDHCYIEGEVDFIFGNAKAVFHDCEIHSVVHEAGGYL 210 Query: 206 TAQSKAAPDEDSAYVFEHCILTADSAARDVALGRPWRPYATVVFLSTKLDAPVVAEGWRE 265 TAQS+ + EDS YVF+HC +TA+ V LGRPWR YATV FL+T+L A + GW E Sbjct: 211 TAQSRNSVAEDSGYVFDHCRVTAEPGVSKVYLGRPWRDYATVTFLNTELRAHIAPAGWSE 270 Query: 266 WAPGKTNSLRTAYYAEYKSTGVGANPAGREPYSHQLTDGEAAKWSLKAFFAGATDWLSA 324 W G+T+ L+TA YAEY+STG GAN A REP S QLT EA + +K + AG+ W +A Sbjct: 271 WHQGETDRLKTASYAEYRSTGPGANVAEREPLSKQLTADEAKGYEVKKYLAGSDGWDAA 329