BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0003.p (627 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_001241401.1| hypothetical protein BBta_5535 [Bradyrhizobi... 615 e-173 ref|YP_001206976.1| putative flagellin and related hook-associat... 609 e-172 ref|NP_772492.1| hypothetical protein bll5852 [Bradyrhizobium ja... 560 e-157 ref|YP_576617.1| hypothetical protein Nham_1333 [Nitrobacter ham... 405 e-110 ref|ZP_08630682.1| flagellar hook-associated protein FlgL [Brady... 342 1e-91 >ref|YP_001241401.1| hypothetical protein BBta_5535 [Bradyrhizobium sp. BTAi1] gb|ABQ37495.1| hypothetical protein BBta_5535 [Bradyrhizobium sp. BTAi1] Length = 623 Score = 615 bits (1585), Expect = e-173, Method: Compositional matrix adjust. Identities = 343/627 (54%), Positives = 451/627 (71%), Gaps = 7/627 (1%) Query: 1 MSIDGVSGRTSYIGTTILNLRNQLNDLTTQLATGKVSTTYAGQGLDRGFALSLRAQISGI 60 MSI ++ +S +G ++ N+ +QL DL+TQL++G + YAG G D GFA++ RAQ+S I Sbjct: 1 MSISSINYGSSLLGLSVRNITSQLADLSTQLSSGTKANNYAGMGPDEGFAIAARAQLSNI 60 Query: 61 NAFSDTATNLNTRLSVANLTLQGLSDVGTQVKNAATTATLTLNDSGQTSGQITAQAAFAN 120 +AFS T T++NT + N +LQ LS +G QV++ A+ L SGQT+GQ TA + + Sbjct: 61 SAFSLTMTHVNTVIGAVNTSLQSLSKIGGQVQSGASATPQNLTSSGQTTGQQTASSNLSA 120 Query: 121 AVAMLNSQSGDRYLFSGRAMDTPATVSADTMLKGTATQAGLSQLINERKQADQGAGMMGR 180 V +LN+Q GDR++FSG A D PA SAD +L GT TQAGL Q+I ER+QAD G +GR Sbjct: 121 IVGILNTQVGDRFIFSGSATDRPALASADDILNGTNTQAGLKQVIAERRQADLGLNGLGR 180 Query: 181 LSVSSPPATTTVTSIAED--GSPFGLKLLSVQSSLTGGATVTQPTGTPKTSSVDLGAVNP 238 L++S+ AT +T ++ED GSPFG KL SV SSLT ATVT P+G+P SVDL A NP Sbjct: 181 LNLSAS-ATMPIT-LSEDVAGSPFGFKLGSVTSSLTN-ATVTGPSGSPAGISVDLTAGNP 237 Query: 239 KDGDKIKFNFTLPDGTTEAIELTATTTNPPPAGSFLIGADTAATTANLQSTLTSSIKTLS 298 GD+I F F LPDGT+E+I+LTA+TT P PA +F IGA AAT+ANL + L +++ TL+ Sbjct: 238 NAGDQISFTFKLPDGTSESIKLTASTTTPAPANTFAIGATPAATSANLNAALNTALSTLA 297 Query: 299 DTSLVAASAVAASNNFFNPVATVSGAAVNNQNTPPAPISGATALSGAAGTDSLSTSFAAG 358 TSLVAASAV A +NFFN ++VSG AVNN+ PAPI+GAT LSGAA TDS++T++ AG Sbjct: 298 GTSLVAASAVTAGDNFFNTASSVSGTAVNNKAATPAPITGATLLSGAASTDSIATNYTAG 357 Query: 359 DTITVNGTPITFVTSGATG-NQVNVTDSVQTLMAKIDQISGTKNPSTISNGAITLHGADG 417 DTITVNG +TFV SGA G NQ+NVTDSVQTL++KID +SGT PST+S G ITL D Sbjct: 358 DTITVNGQTLTFVASGANGNNQINVTDSVQTLLSKIDALSGTSKPSTVSGGVITLRTDDA 417 Query: 418 ESLSISSSNTAALGALGIANNTTAAPAPLRVGGPPFDTATNLVAGTPGNTVYWYTGENGP 477 S S+SSSN A ALG + TAA PLRV G P +AT+LV G+ TV WYTG +GP Sbjct: 418 ASFSVSSSNAGAFAALGFSGTVTAATPPLRVSGSPLGSATSLVNGS-ATTVNWYTGNSGP 476 Query: 478 GSARGTAVAQVDQSITVQYGARANEQAFRYLLQNVAVYAAVTTNASNPNASAQVTALAQR 537 G+AR ++ ++D + ++Q+GA+ANE A R L+Q AVYAAV T + NA Q+ AL+QR Sbjct: 477 GTARSSSTIRIDTAQSIQFGAQANELAIRNLVQQTAVYAAVNTPPAATNAGPQIAALSQR 536 Query: 538 VSANLAPQNGQQQIQDVQAEFAGAQTATKAATDRQTQLKGMAETMLDSVEGVNQDEVATK 597 ++ANL PQ GQQ I D+Q +FA AQT K AT RQTQ K ++++D EG++Q+EVA++ Sbjct: 537 IAANLTPQLGQQSISDIQTDFANAQTTMKDATARQTQAKAAMQSLVDQTEGISQNEVASQ 596 Query: 598 ILALQTSLQASYQTTSMLYQTTLLKYL 624 IL LQT+LQASYQTT+ML Q +L+K+L Sbjct: 597 ILQLQTNLQASYQTTAMLAQLSLVKFL 623 >ref|YP_001206976.1| putative flagellin and related hook-associated protein [Bradyrhizobium sp. ORS 278] emb|CAL78759.1| Conserved hypothetical protein; putative Flagellin and related hook-associated protein [Bradyrhizobium sp. ORS 278] Length = 623 Score = 609 bits (1571), Expect = e-172, Method: Compositional matrix adjust. Identities = 340/627 (54%), Positives = 449/627 (71%), Gaps = 7/627 (1%) Query: 1 MSIDGVSGRTSYIGTTILNLRNQLNDLTTQLATGKVSTTYAGQGLDRGFALSLRAQISGI 60 M+I V+ TS +G ++ N+ NQL DL+TQL++G ++ YAG G D GFA++ RAQ+S I Sbjct: 1 MTISSVNYGTSLLGLSVRNISNQLADLSTQLSSGNKASNYAGMGPDEGFAIAARAQLSNI 60 Query: 61 NAFSDTATNLNTRLSVANLTLQGLSDVGTQVKNAATTATLTLNDSGQTSGQITAQAAFAN 120 +AF+ T T++NT + N +LQ LS +G QV++ A+ L +GQT+GQ TA + + Sbjct: 61 SAFTLTMTHVNTVIGAMNTSLQSLSKIGDQVQSGASATPQNLTSTGQTTGQQTASSNLSA 120 Query: 121 AVAMLNSQSGDRYLFSGRAMDTPATVSADTMLKGTATQAGLSQLINERKQADQGAGMMGR 180 V +LN+Q GDR++FSG A D PA SAD +L GTATQAGL Q+I ER+QAD G +GR Sbjct: 121 IVGILNTQVGDRFIFSGSATDRPAIASADEILNGTATQAGLKQVIAERRQADLGLNGLGR 180 Query: 181 LSVSSPPATTTVTSIAED--GSPFGLKLLSVQSSLTGGATVTQPTGTPKTSSVDLGAVNP 238 L++S+ AT +T ++ED GSPFG KL SV SSLT ATVT P+G+P SVDL A NP Sbjct: 181 LNLSAS-ATVPIT-LSEDVAGSPFGFKLSSVTSSLTN-ATVTGPSGSPAGISVDLTAGNP 237 Query: 239 KDGDKIKFNFTLPDGTTEAIELTATTTNPPPAGSFLIGADTAATTANLQSTLTSSIKTLS 298 GD+I F F LPDGT+E+I+LTA+TT P PA +F IG AAT+ANL + L ++I TL+ Sbjct: 238 NAGDQISFTFKLPDGTSESIQLTASTTTPAPANTFAIGLTPAATSANLNAALNTAIGTLA 297 Query: 299 DTSLVAASAVAASNNFFNPVATVSGAAVNNQNTPPAPISGATALSGAAGTDSLSTSFAAG 358 +T+LV ASAV A +NFFN + VSG VNN+ PAPI+GAT LSGAA TDS+ST+FA+G Sbjct: 298 NTALVGASAVTAGDNFFNTASAVSGTVVNNKAATPAPITGATLLSGAASTDSISTNFASG 357 Query: 359 DTITVNGTPITFVTSGATGN-QVNVTDSVQTLMAKIDQISGTKNPSTISNGAITLHGADG 417 DTITVNG ++FVTSGA+GN Q+N+TDSVQTL++KID +SGT ST+S GAITL + Sbjct: 358 DTITVNGQTLSFVTSGASGNTQINITDSVQTLLSKIDALSGTSKASTVSGGAITLRTDNA 417 Query: 418 ESLSISSSNTAALGALGIANNTTAAPAPLRVGGPPFDTATNLVAGTPGNTVYWYTGENGP 477 S SISSSN A ALG + TA PLRV G P +AT LV G+ NT+ WYTG +GP Sbjct: 418 ASFSISSSNAGAFAALGFSGTVTAPTPPLRVSGTPLGSATTLVNGST-NTISWYTGNSGP 476 Query: 478 GSARGTAVAQVDQSITVQYGARANEQAFRYLLQNVAVYAAVTTNASNPNASAQVTALAQR 537 G+AR +A ++D + ++Q+GA+ANE A R L+Q AVYAAV T + NA Q+ AL++R Sbjct: 477 GTARASASIRIDTAQSIQFGAQANEFAIRNLVQQTAVYAAVNTPPTGANAGQQIGALSER 536 Query: 538 VSANLAPQNGQQQIQDVQAEFAGAQTATKAATDRQTQLKGMAETMLDSVEGVNQDEVATK 597 ++ NL Q GQQ I D+Q +FA AQT K AT RQTQ K ++++D EG++Q EVA++ Sbjct: 537 IAYNLTSQLGQQSISDIQTDFANAQTTMKDATARQTQAKAAMQSLVDQTEGISQQEVASQ 596 Query: 598 ILALQTSLQASYQTTSMLYQTTLLKYL 624 IL LQT+LQASYQTT+ML Q +L+KYL Sbjct: 597 ILQLQTNLQASYQTTAMLAQLSLVKYL 623 >ref|NP_772492.1| hypothetical protein bll5852 [Bradyrhizobium japonicum USDA 110] dbj|BAC51117.1| bll5852 [Bradyrhizobium japonicum USDA 110] Length = 627 Score = 560 bits (1443), Expect = e-157, Method: Compositional matrix adjust. Identities = 318/619 (51%), Positives = 424/619 (68%), Gaps = 11/619 (1%) Query: 13 IGTTILNLRNQLNDLTTQLATGKVSTTYAGQGLDRGFALSLRAQISGINAFSDTATNLNT 72 +G+ I N+ QL DL+TQL+TGK+S Y+G G + GFA++ RAQ+S I A++DT TN+N Sbjct: 13 LGSQIRNINQQLTDLSTQLSTGKLSQNYSGMGTNEGFAIAGRAQLSNIAAYTDTITNVNV 72 Query: 73 RLSVANLTLQGLSDVGTQVKNAATTATLTLNDSGQTSGQITAQAAFANAVAMLNSQSGDR 132 +++AN LQ L+ + V+ + LN +GQT Q TA A F + V +LN+Q+G+R Sbjct: 73 SINLANTALQSLTKIRNTVQTGSANTAQDLNVNGQTVAQNTAAAQFGSMVGVLNTQTGNR 132 Query: 133 YLFSGRAMDTPATVSADTMLKGTATQAGLSQLINERKQADQGAGMMGRLSVSSPPATTTV 192 YLFSG A++T + A ++ GT TQAG ++ ER+ AD GA MGRL + P T + Sbjct: 133 YLFSGTAVNTQSVADAGDIINGTTTQAGFKAVMAERQAADLGATGMGRLVQTQP--TPSS 190 Query: 193 TSIAED--GSPFGLKLLSVQSSLTGGATVTQPTGTPKTSSVDLGAVNPKDGDKIKFNFTL 250 ++ED GSPFGLK+ +V S+LTG ATVT P+G+P + SVDL NP +GDK+ FTL Sbjct: 191 VQVSEDVAGSPFGLKIKAVSSTLTG-ATVTGPSGSPVSFSVDLNGTNPSNGDKLSVQFTL 249 Query: 251 PDGTTEAIELTATTTNPPPAGSFLIGADTAA----TTANLQSTLTSSIKTLSDTSLVAAS 306 PDGTTE I+LTA+T P P GSF I A T ANL + L ++IK LS+TSLVAAS Sbjct: 250 PDGTTEQIDLTASTATPTPLGSFAIDASVPVNPNNTAANLNTALNTAIKKLSNTSLVAAS 309 Query: 307 AVAASNNFFNPVATVSGAAVNNQNTPPAPISGATALSGAAGTDSLSTSFAAGDTITVNGT 366 AV A +NFFN ++ G +NQ PPAPISGATAL G + +DS+S F AGDTITVNGT Sbjct: 310 AVTAGDNFFNTASSAIGTPKSNQAAPPAPISGATALFGTSPSDSISPGFVAGDTITVNGT 369 Query: 367 PITFVTSGATGNQVNVTDSVQTLMAKIDQISGTKNPSTISNGAITLHGADGESLSISSSN 426 +TFV+SGATGNQ+NV DS+QTLM+KIDQI+GT PSTI G+IT++ D +L+I+SSN Sbjct: 370 TLTFVSSGATGNQLNVGDSIQTLMSKIDQITGTSKPSTIHGGSITINTDDAATLNITSSN 429 Query: 427 TAALGALGIANN-TTAAPAPLRVGGPPFDTATNLVAGTPGNTVYWYTGENGPGSARGTAV 485 T ALG+LG + TA PLRVG P +AT LV G+ TV WY G +GPGS R TA+ Sbjct: 430 TGALGSLGFSTTPVTATQPPLRVGSSPASSATTLVNGS-ATTVKWYLGNDGPGSPRSTAM 488 Query: 486 AQVDQSITVQYGARANEQAFRYLLQNVAVYAAVTTNASNPNASAQVTALAQRVSANLAPQ 545 A+VD S+TVQYGA+A+E A R LQ +AV+ +T+ + + QV+AL+ RV+ L Q Sbjct: 489 ARVDDSVTVQYGAQADEDAIRRQLQAIAVFGTFSTSPTGQYSGGQVSALSLRVTQALTQQ 548 Query: 546 NGQQQIQDVQAEFAGAQTATKAATDRQTQLKGMAETMLDSVEGVNQDEVATKILALQTSL 605 GQQ+I+D+Q + A AQ K A+ RQTQ K +T++D E D+VA++ILALQ +L Sbjct: 549 PGQQRIEDIQTDIAMAQNTMKDASTRQTQAKAQLQTIIDQAESAPPDQVASEILALQNAL 608 Query: 606 QASYQTTSMLYQTTLLKYL 624 QASYQ TS L Q +L+K+L Sbjct: 609 QASYQVTSKLAQLSLVKFL 627 >ref|YP_576617.1| hypothetical protein Nham_1333 [Nitrobacter hamburgensis X14] gb|ABE62157.1| hypothetical protein Nham_1333 [Nitrobacter hamburgensis X14] Length = 587 Score = 405 bits (1040), Expect = e-110, Method: Compositional matrix adjust. Identities = 273/631 (43%), Positives = 384/631 (60%), Gaps = 51/631 (8%) Query: 1 MSIDGVSGRTSYIGTTILNLRNQLNDLTTQLATGKVSTTYAGQGLDRGFALSLRAQISGI 60 M+I+G+S + +G +I +L+ Q+ DL +QL +GK STTY+G G + GFA++ RAQ+S I Sbjct: 1 MAINGISFGNTILGGSIQSLKVQMADLQSQLTSGKKSTTYSGMGANEGFAIAARAQLSNI 60 Query: 61 NAFSDTATNLNTRLSVANLTLQGLSDVGTQVKNAATTATLTLNDSGQTSGQITAQAAFAN 120 A++DT T +NT + VAN LQ L+D+G+Q++ AA + + LN +GQT+GQ TA A F++ Sbjct: 61 AAYTDTMTKINTNIQVANTALQALADIGSQLQTAANSGSQALNSAGQTAGQQTAVAEFSS 120 Query: 121 AVAMLNSQSGDRYLFSGRAMDTPATVSADTMLKGT-ATQAGLSQLINERKQADQGAGMMG 179 V +LN+Q+GDRY+FSG + T S+D +L G A QAGL Q+I+ER QAD G G G Sbjct: 121 MVGILNTQAGDRYIFSGSTIYTQPVASSDLILNGNGAGQAGLKQVISERNQADLGTGGFG 180 Query: 180 RLSVSSPPATTTVTSIAEDGSP--FGLKLLSVQSSLTGGATVTQPTGTPKTSSVDLGAVN 237 R+++SSP TTT S+AED SP +GLKL V S+LTG ATVT TP T V+L ++ Sbjct: 181 RVTISSP--TTTSVSVAEDASPSVYGLKLSKVTSTLTG-ATVTN---TPAT--VNLASI- 231 Query: 238 PKDGDKIKFNFTLPDGTTEAIELTATTTNPPPAGSFLIGADTAATTANLQSTLTSSIKTL 297 P DGD F F DGT+ ++LTATTT P PAGSF IGA AA+ NL + LT + T Sbjct: 232 PNDGDTFNFTFLQADGTSATVQLTATTTVPAPAGSFEIGATAAASATNLNAALTDAEAT- 290 Query: 298 SDTSLVAASAVAASNNFFNPVATVSGAAVNNQNTPPAPISGATALSGAAGTDSLSTSFAA 357 TS A S++ ++G T A D + + AA Sbjct: 291 --TSPYGAQLTGVSSS----------------------VAGVTVTPAQASIDLGAATPAA 326 Query: 358 GDTITVNGTPITFVTSGATGNQVNVTDSVQTLMAKIDQISGTKNPSTISNGAITLHGADG 417 GDT++ TF + V +T S + GT T +N L+GA Sbjct: 327 GDTVS-----FTFNLPDGSSETVQLTASSEVPTPGGCFAIGTTPDETTAN----LNGALT 377 Query: 418 ESL-SISSSNTAALGALGIANNTTAAPAPLRVGGPP---FDTATNLVAGTPGNTVYWYTG 473 +S+ +++ ++ A A+ ++ +P P RV P TAT V GT NTV WYTG Sbjct: 378 KSIGTLAKTSLVAASAIRATHDFFDSP-PQRVDVTPPATLATATGAVNGTAANTVQWYTG 436 Query: 474 ENGPGSARGTAVAQVDQSITVQYGARANEQAFRYLLQNVAVYAAVTTNASNPNASAQVTA 533 + G GSAR T+ +VD S+TVQYG RANE A R L+++VAV AAVTT+ ++PN + Q++A Sbjct: 437 DAGTGSARATSSVRVDPSVTVQYGIRANEDAIRSLMESVAVLAAVTTSPTDPNGANQISA 496 Query: 534 LAQRVSANLAPQNGQQQIQDVQAEFAGAQTATKAATDRQTQLKGMAETMLDSVEGVNQDE 593 L R++ NL G+Q IQD+Q++ A AQT K AT RQ + + ++D E V+ D+ Sbjct: 497 LNARIAQNLTVTPGKQNIQDIQSDLANAQTVMKDATARQKLTQATMQNIVDQAETVSTDQ 556 Query: 594 VATKILALQTSLQASYQTTSMLYQTTLLKYL 624 VA+++LALQTSLQASYQTT+ML Q +L+K+L Sbjct: 557 VASQLLALQTSLQASYQTTAMLSQLSLVKFL 587 >ref|ZP_08630682.1| flagellar hook-associated protein FlgL [Bradyrhizobiaceae bacterium SG-6C] gb|EGP06536.1| flagellar hook-associated protein FlgL [Bradyrhizobiaceae bacterium SG-6C] Length = 496 Score = 342 bits (876), Expect = 1e-91, Method: Compositional matrix adjust. Identities = 190/317 (59%), Positives = 248/317 (78%), Gaps = 6/317 (1%) Query: 1 MSIDGVSGRTSYIGTTILNLRNQLNDLTTQLATGKVSTTYAGQGLDRGFALSLRAQISGI 60 M+I+GVSGRTSY+G++I+NL++QL+DL+ QLA+GK + TY+G G+D G A LRAQ++GI Sbjct: 1 MTIEGVSGRTSYLGSSIINLKSQLDDLSQQLASGKKTNTYSGLGVDSGVATGLRAQLAGI 60 Query: 61 NAFSDTATNLNTRLSVANLTLQGLSDVGTQVKNAATTATLTLNDSGQTSGQITAQAAFAN 120 A ++T T LNTR+++ANL+LQG+SD +QVK AA ATLTL+++GQT+GQ TA A+F Sbjct: 61 TANNNTGTLLNTRINIANLSLQGISDASSQVKGAAAGATLTLDNTGQTAGQKTAMASFTQ 120 Query: 121 AVAMLNSQSGDRYLFSGRAMDTPATVSADTMLKGTATQAGLSQLINERKQADQGAGMMGR 180 +A+LN++ G RYLFSGRA DTPAT AD +L GT TQAGL QLI+ER QAD G +GR Sbjct: 121 VIALLNTEVGGRYLFSGRATDTPATQPADVILNGTGTQAGLKQLIDERAQADGTTG-LGR 179 Query: 181 LSVSSPPATTTVTSIAED--GSPFGLKLLSVQSSLTGGATVTQPTGTPKTSSVDLGAVNP 238 ++SP T T +I ED GSPFG KL S+ S+LT G+TVT P G+P SVDLGA NP Sbjct: 180 TVITSP--TPTSVTIGEDAAGSPFGFKLSSITSTLT-GSTVTGPAGSPPEMSVDLGATNP 236 Query: 239 KDGDKIKFNFTLPDGTTEAIELTATTTNPPPAGSFLIGADTAATTANLQSTLTSSIKTLS 298 +DGDK++F F+LPDGT+E I LTAT + P PAGSF IGAD+ AT +NL + L +++ TL+ Sbjct: 237 QDGDKVRFTFSLPDGTSETIVLTATASTPVPAGSFAIGADSTATASNLNAALNTAVGTLA 296 Query: 299 DTSLVAASAVAASNNFF 315 +TSLVAASAVAASN+FF Sbjct: 297 NTSLVAASAVAASNDFF 313 Score = 217 bits (553), Expect = 4e-54, Method: Compositional matrix adjust. Identities = 112/180 (62%), Positives = 137/180 (76%), Gaps = 6/180 (3%) Query: 451 PP--FDTATN----LVAGTPGNTVYWYTGENGPGSARGTAVAQVDQSITVQYGARANEQA 504 PP DT+TN L GTP +TV WY GENG ARGTAVA +D SITVQYGARANE+A Sbjct: 317 PPQRVDTSTNPPVALRNGTPTDTVSWYIGENGSDPARGTAVAGIDDSITVQYGARANEEA 376 Query: 505 FRYLLQNVAVYAAVTTNASNPNASAQVTALAQRVSANLAPQNGQQQIQDVQAEFAGAQTA 564 F L+N+AVYAAVTTNAS+PNA+ Q+TAL R+ NLA G Q I+D+QA+FAG Q A Sbjct: 377 FVAALKNIAVYAAVTTNASSPNANGQLTALNSRIVDNLAVHPGTQSIEDIQADFAGTQAA 436 Query: 565 TKAATDRQTQLKGMAETMLDSVEGVNQDEVATKILALQTSLQASYQTTSMLYQTTLLKYL 624 K A DRQTQ +A+ MLD+++G++ +EVA KILALQTSLQASYQTT+ LYQ +L+K+L Sbjct: 437 IKTAKDRQTQTGAVAQAMLDTIQGIDDNEVAAKILALQTSLQASYQTTASLYQMSLVKFL 496