BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0038.p (344 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_774091.1| hypothetical protein blr7451 [Bradyrhizobium ja... 602 e-170 ref|YP_003796175.1| d-alanine-d-alanine ligase [Candidatus Nitro... 382 e-104 ref|NP_870957.1| D-alanine-D-alanine ligase [Rhodopirellula balt... 353 2e-95 emb|CBW27144.1| putative D-alanine--D-alanine ligase [Bacteriovo... 325 7e-87 ref|YP_001819045.1| D-alanine--D-alanine ligase domain-containin... 281 1e-73 >ref|NP_774091.1| hypothetical protein blr7451 [Bradyrhizobium japonicum USDA 110] dbj|BAC52716.1| blr7451 [Bradyrhizobium japonicum USDA 110] Length = 348 Score = 602 bits (1553), Expect = e-170, Method: Compositional matrix adjust. Identities = 290/344 (84%), Positives = 311/344 (90%) Query: 1 MKQLRILVLMHPDCVPPDSSDGYSAREINEWKTEYDVVSTLRAAGHEVRPLGVQEEIKPV 60 M++LRILVLMHPD +PPDSSDGY+A+EIN WKTEYDVVSTLRAAGHEVR LG Q+EIKPV Sbjct: 5 MRRLRILVLMHPDFMPPDSSDGYTAQEINAWKTEYDVVSTLRAAGHEVRALGAQDEIKPV 64 Query: 61 RDEIEGFKPHVVFTLLEEFHYDVVYDQHIASYLELMKIAYTGCNPRGLILARGKDLSKTL 120 R+ IE FKPHVVFTLLEEFH +V YDQHIASYLELMK+ YTGCNPRGLILARGKDLSKTL Sbjct: 65 REAIEEFKPHVVFTLLEEFHNNVAYDQHIASYLELMKVPYTGCNPRGLILARGKDLSKTL 124 Query: 121 VHHRRIPVPAFAVFPMRRKVKRPPRLELPLIVKSLSSDGSLGISQASIVDTDEKLAERVA 180 VHHRRI PAFAVFPMRRKVKRP RL LPLIVKSL+ DGS GISQASIVDTDEKLAERVA Sbjct: 125 VHHRRIAAPAFAVFPMRRKVKRPTRLALPLIVKSLNMDGSFGISQASIVDTDEKLAERVA 184 Query: 181 FIHERIGTAAIAEQFIEGRELYVGVLGNNRLRVLPVWELKFGSMGGPAARQIATEKVKHD 240 FIHER+ +AAIAEQFIEGRELYVGVLGNNRLRVLP+WELKFGSMGG +R IATEK KHD Sbjct: 185 FIHERVESAAIAEQFIEGRELYVGVLGNNRLRVLPIWELKFGSMGGRRSRHIATEKAKHD 244 Query: 241 TSYQERVGIADGPAEDLTPELSAHIQRLAKRIYRTLGLDGYARIDFRLSTNGVPYFIEAN 300 T YQE+VGI DGPA+DL PE++A IQR AKRIYR LGLDGYARIDFRL+ +G PYFIEAN Sbjct: 245 TDYQEKVGIVDGPAKDLAPEVTARIQRAAKRIYRALGLDGYARIDFRLAADGTPYFIEAN 304 Query: 301 PNPEIAKSQEFATAAGHDGLNYPDLLQRIVTLGISRAKAGASLG 344 PNPEIAKSQEFATAA H GL YPDLLQRI+ LGISRAKAG SLG Sbjct: 305 PNPEIAKSQEFATAAQHAGLKYPDLLQRILALGISRAKAGVSLG 348 >ref|YP_003796175.1| d-alanine-d-alanine ligase [Candidatus Nitrospira defluvii] emb|CBK40249.1| D-alanine-D-alanine ligase [Candidatus Nitrospira defluvii] Length = 340 Score = 382 bits (980), Expect = e-104, Method: Compositional matrix adjust. Identities = 191/342 (55%), Positives = 243/342 (71%), Gaps = 2/342 (0%) Query: 1 MKQLRILVLMHPDCVPPDSSDGYSAREINEWKTEYDVVSTLRAAGHEVRPLGVQEEIKPV 60 M++LR+LVLMH D VPPD +G E WKTEYDVVSTLR GHEV+PLGV+ +++ + Sbjct: 1 MRRLRVLVLMHKDLVPPDQLNGQHL-EGAAWKTEYDVVSTLRKLGHEVQPLGVKSDLEVI 59 Query: 61 RDEIEGFKPHVVFTLLEEFHYDVVYDQHIASYLELMKIAYTGCNPRGLILARGKDLSKTL 120 R +E +KPH+ F LLEEF VYDQ++ SYLELM+I YTGCNPRGL+LAR K L+K + Sbjct: 60 RAAVEDWKPHIAFNLLEEFDGRAVYDQNVVSYLELMRIPYTGCNPRGLMLARDKALAKQV 119 Query: 121 VHHRRIPVPAFAVFPMRRKVKRPPRLELPLIVKSLSSDGSLGISQASIVDTDEKLAERVA 180 + + RIP P F V P+ R V+RP L PLIVKS+S + SLGISQASIVD DEKL ERVA Sbjct: 120 MSYHRIPYPEFMVVPLHRMVRRPKYLPFPLIVKSISEEASLGISQASIVDDDEKLRERVA 179 Query: 181 FIHERIGTAAIAEQFIEGRELYVGVLGNNRLRVLPVWELKFGSMGGPAARQIATEKVKHD 240 FIH+ +GT A+ E++IEGRELYVGV+GN L+V PVWEL M AR+IATE+VK Sbjct: 180 FIHDNVGTGALVERYIEGRELYVGVMGNAHLQVFPVWELVMDKM-PEEARRIATERVKWS 238 Query: 241 TSYQERVGIADGPAEDLTPELSAHIQRLAKRIYRTLGLDGYARIDFRLSTNGVPYFIEAN 300 YQ++ GI A +L + H+Q LAKR+YR LGL GYARID R+ +G Y +EAN Sbjct: 239 RKYQDKYGICSCEARNLPDGVVDHVQHLAKRVYRALGLSGYARIDMRMDQDGNVYVLEAN 298 Query: 301 PNPEIAKSQEFATAAGHDGLNYPDLLQRIVTLGISRAKAGAS 342 PNP+IA ++FA +A L Y DLLQ ++ +G+ A A+ Sbjct: 299 PNPQIASGEDFADSAEKADLAYKDLLQELLHVGLRWRPAQAA 340 >ref|NP_870957.1| D-alanine-D-alanine ligase [Rhodopirellula baltica SH 1] emb|CAD78035.1| probable D-alanine-D-alanine ligase [Rhodopirellula baltica SH 1] gb|EGF25995.1| D-alanine-D-alanine ligase [Rhodopirellula baltica WH47] Length = 341 Score = 353 bits (907), Expect = 2e-95, Method: Compositional matrix adjust. Identities = 173/335 (51%), Positives = 226/335 (67%), Gaps = 1/335 (0%) Query: 1 MKQLRILVLMHPDCVPPDSSDGYSAREINEWKTEYDVVSTLRAAGHEVRPLGVQEEIKPV 60 M +LR+LVL+ VPPD+ +G++ +E + WK EYDV TLR GHEV PLG+ +++ P+ Sbjct: 1 MSKLRVLVLVREGNVPPDTLEGFTDKESDPWKAEYDVCETLRGLGHEVLPLGIFDDLAPI 60 Query: 61 RDEIEGFKPHVVFTLLEEFHYDVVYDQHIASYLELMKIAYTGCNPRGLILARGKDLSKTL 120 R ++ F+P++ + LLEEF+ V YD + SYLELM+ Y+GCNPRGL+L++ K LSK L Sbjct: 61 RAALQNFQPNITYMLLEEFYGVVTYDFAVISYLELMQQPYSGCNPRGLMLSKDKALSKKL 120 Query: 121 VHHRRIPVPAFAVFPMRRKVKRPPRLELPLIVKSLSSDGSLGISQASIVDTDEKLAERVA 180 + + RI P FAVFP R V+RP +LE PL VKS D S GI+QASIV DE LAERV Sbjct: 121 LMYHRIATPRFAVFPNGRAVRRPKKLEFPLFVKSTIEDASFGIAQASIVHNDEALAERVT 180 Query: 181 FIHERIGTAAIAEQFIEGRELYVGVLGNNRLRVLPVWELKFGSMGGPAARQIATEKVKHD 240 F+HE+ G I EQ+IEGRELYVGV+GN RL P WE+ FG M +AR IAT +VK D Sbjct: 181 FLHEKTGGDVIVEQYIEGRELYVGVMGNTRLVTFPAWEMDFGKMPDESAR-IATSRVKWD 239 Query: 241 TSYQERVGIADGPAEDLTPELSAHIQRLAKRIYRTLGLDGYARIDFRLSTNGVPYFIEAN 300 YQE+ I AE LT HI +L KR+YR L + GYAR+D R++ G Y IEAN Sbjct: 240 RKYQEKHDITCHAAEGLTEVQQKHIAKLCKRVYRALHMSGYARMDLRMTATGEVYVIEAN 299 Query: 301 PNPEIAKSQEFATAAGHDGLNYPDLLQRIVTLGIS 335 NP I ++FA +A G+ Y L+QRI+ LG+S Sbjct: 300 ANPNIEYGEDFAESAEAAGIPYESLIQRILNLGLS 334 >emb|CBW27144.1| putative D-alanine--D-alanine ligase [Bacteriovorax marinus SJ] Length = 336 Score = 325 bits (833), Expect = 7e-87, Method: Compositional matrix adjust. Identities = 159/337 (47%), Positives = 226/337 (67%), Gaps = 5/337 (1%) Query: 4 LRILVLMHPDCVPPD--SSDGYSAREINEWKTEYDVVSTLRAAGHEVRPLGVQEEIKPVR 61 +++LVLMH D VPP D + E+ W TEYDV+ L GH+V GV ++K ++ Sbjct: 1 MKVLVLMHKDLVPPTDIKEDQFDKDEV-PWITEYDVIKALNNLGHKVEICGVISDLKLIK 59 Query: 62 DEIEGFKPHVVFTLLEEFHYDVVYDQHIASYLELMKIAYTGCNPRGLILARGKDLSKTLV 121 + I+ FKP V+F LLEEF +V++DQ++ SYLEL+++ YTGCNPRGL+LAR K L+K ++ Sbjct: 60 NAIDKFKPTVIFNLLEEFDGEVLFDQNVVSYLELLRVPYTGCNPRGLMLARDKALAKKIL 119 Query: 122 HHRRIPVPAFAVFPMRRKVKRPPRLELPLIVKSLSSDGSLGISQASIVDTDEKLAERVAF 181 RI P F VFP RKVK L+ PLIVK L+ + SL I++ASIV +EKL ERV + Sbjct: 120 LFHRIKTPHFQVFPKNRKVKTSKHLKYPLIVKCLTEEASLAIAKASIVYNEEKLKERVKY 179 Query: 182 IHERIGTAAIAEQFIEGRELYVGVLGNNRLRVLPVWELKFGSMGGPAARQIATEKVKHDT 241 I+++IG AI E+FIEGRE YVGV+GN + + LPVWELKF ++ P +++ + + K + Sbjct: 180 INQKIGVDAIVEEFIEGREFYVGVMGNYKTQTLPVWELKFENVEKP-EKELYSNRAKWNN 238 Query: 242 SYQERVGIADGPAEDLTPELSAHIQRLAKRIYRTLGLDGYARIDFRLSTNGVPYFIEANP 301 Y++R GI A L+PEL I+ + K++Y+ L L+GYAR+D R++ G Y IE NP Sbjct: 239 KYRDRKGIK-TEAATLSPELELKIKNICKKVYKHLNLNGYARVDLRMNPAGEVYVIEVNP 297 Query: 302 NPEIAKSQEFATAAGHDGLNYPDLLQRIVTLGISRAK 338 NP IA+ E A++A H +NY L+ +I+ L S AK Sbjct: 298 NPNIAREDELASSALHSKVNYEQLITKILKLAKSWAK 334 >ref|YP_001819045.1| D-alanine--D-alanine ligase domain-containing protein [Opitutus terrae PB90-1] gb|ACB75445.1| D-alanine--D-alanine ligase domain protein [Opitutus terrae PB90-1] Length = 339 Score = 281 bits (719), Expect = 1e-73, Method: Compositional matrix adjust. Identities = 144/336 (42%), Positives = 213/336 (63%), Gaps = 5/336 (1%) Query: 2 KQLRILVLMHPDCVPPDSSDGY--SAREINEWKTEYDVVSTLRAAGHEVRPLGVQEEIKP 59 ++L++L L D P +D + + EWKTE V++ L G+ L + ++++P Sbjct: 3 RKLKVLALF--DVGEPTRADEDLGATLQTPEWKTEAGVLAALNELGYPNEHLAIFDDLEP 60 Query: 60 VRDEIEGFKPHVVFTLLEEFHYDVVYDQHIASYLELMKIAYTGCNPRGLILARGKDLSKT 119 +R +++ F+P VVF L ++F + +DQHI S+L + I +TGC GL L + K +SK Sbjct: 61 LRQKVQAFQPDVVFNLADQFRNNRAFDQHIVSFLAMSDIPFTGCGSTGLTLCKHKGVSKK 120 Query: 120 LVHHRRIPVPAFAVFPMRRKVKRPPRLELPLIVKSLSSDGSLGISQASIVDTDEKLAERV 179 ++ + RI PAFA+ P R+ RP RL+ P++VK L + S GI+QAS V+TDE+ ERV Sbjct: 121 ILSYHRIHTPAFAIIPRGRRAVRPKRLKFPILVKPLKEEASYGIAQASFVETDEQFRERV 180 Query: 180 AFIHERIGTAAIAEQFIEGRELYVGVLGNNRLRVLPVWELKFGSMGGPAARQIATEKVKH 239 F+H++ AIAE++IEGRELYV +LGN+RL+V P+ EL F + P +IAT K K Sbjct: 181 EFVHQKFNNDAIAEEYIEGRELYVSLLGNHRLQVFPIRELVFREV-PPDEPKIATYKAKW 239 Query: 240 DTSYQERVGIADGPAEDLTPELSAHIQRLAKRIYRTLGLDGYARIDFRLSTNGVPYFIEA 299 D +Y+ R G+ + EDL P L I+ +RIYR L +DGYAR+D RL+ +FIEA Sbjct: 240 DEAYRARWGLENRFVEDLEPALVRKIEATCRRIYRLLTIDGYARLDLRLNARNELWFIEA 299 Query: 300 NPNPEIAKSQEFATAAGHDGLNYPDLLQRIVTLGIS 335 N NP +A ++FA +AG GL+YP L+ RI LG+S Sbjct: 300 NANPILAPDEDFALSAGKAGLSYPQLIDRIARLGLS 335