BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0005.p (354 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_001990754.1| Nitrilase [Rhodopseudomonas palustris TIE-1]... 515 e-144 ref|NP_946909.1| putative nitrilase [Rhodopseudomonas palustris ... 515 e-144 ref|YP_002289780.1| aliphatic nitrilase [Oligotropha carboxidovo... 514 e-144 ref|ZP_07027303.1| Nitrilase/cyanide hydratase and apolipoprotei... 473 e-131 ref|YP_002907672.1| nitrilase [Burkholderia glumae BGR1] >gi|237... 453 e-125 >ref|YP_001990754.1| Nitrilase [Rhodopseudomonas palustris TIE-1] gb|ACF00279.1| Nitrilase [Rhodopseudomonas palustris TIE-1] Length = 349 Score = 515 bits (1327), Expect = e-144, Method: Compositional matrix adjust. Identities = 247/333 (74%), Positives = 281/333 (84%), Gaps = 1/333 (0%) Query: 1 MLPRFKAAAVHAAPVFLDSWATTEKAIAIIREAARAGAELIAFPETYIPAFPVWAAMWPP 60 MLP FKAAA+HAAPVFLD ATT+KAI++IREA AGAEL+AFPETYIPAFPVWAA+W P Sbjct: 1 MLPHFKAAAIHAAPVFLDKTATTKKAISLIREAVAAGAELVAFPETYIPAFPVWAALWAP 60 Query: 61 IDNHDLFVRMAEQSVLADGPEVARLCAEARALGVTVSIGISERSAVSVGGLWNSNLLIGE 120 IDNHDLFVRMA+QSVL DGPEV + EAR LGV VSIGISE+S SVGG+WNSNLLIGE Sbjct: 61 IDNHDLFVRMADQSVLIDGPEVKAIRDEARRLGVVVSIGISEKSPASVGGIWNSNLLIGE 120 Query: 121 TGEILIHHRKLVPTFYEKLVWSSGDGAGLKVAETRQGRIGGLICGENTNPLARFALMAQG 180 GEIL HHRKLVPTFYEKL+WS+GDGAGL+V +TR G+IG LICGENTNPLAR+ALMAQG Sbjct: 121 DGEILNHHRKLVPTFYEKLIWSAGDGAGLRVVDTRLGKIGQLICGENTNPLARYALMAQG 180 Query: 181 EQVHISSWPPMWPTRRPAGGGNFDNVAANRIRASAHCFEAKVFGIVTAGYMDQAMRAFLT 240 EQ HISSWPP+WPTRRPA GGN+ AA RIRASAHCFEAKVFG+VT+G +D+A R L Sbjct: 181 EQFHISSWPPVWPTRRPAEGGNYHIAAATRIRASAHCFEAKVFGLVTSGVLDKAARDMLV 240 Query: 241 ERDRTIADVIDQTPRAASFFVDPTGSVFGDGLQDEEGIAYAEIDLNRCVEPKQFHDVVGY 300 RD + A V+D TPRAA+FF+DPTG G+ L ++EGI YA+IDL RCVEPKQFHDVVGY Sbjct: 241 ARDPSAAAVLDGTPRAATFFLDPTGEQIGEALCEDEGILYADIDLTRCVEPKQFHDVVGY 300 Query: 301 YNRFDIFGVTVDRSRLTPARFRDADLPIAQVQD 333 YNRFD+F V++ R RLTPA F D DLP+ V D Sbjct: 301 YNRFDVFAVSISRHRLTPATFID-DLPLPAVVD 332 >ref|NP_946909.1| putative nitrilase [Rhodopseudomonas palustris CGA009] emb|CAE27004.1| putative nitrilase [Rhodopseudomonas palustris CGA009] Length = 349 Score = 515 bits (1326), Expect = e-144, Method: Compositional matrix adjust. Identities = 247/333 (74%), Positives = 281/333 (84%), Gaps = 1/333 (0%) Query: 1 MLPRFKAAAVHAAPVFLDSWATTEKAIAIIREAARAGAELIAFPETYIPAFPVWAAMWPP 60 MLP FKAAA+HAAPVFLD ATT+KAI++IREA AGAEL+AFPETYIPAFPVWAA+W P Sbjct: 1 MLPHFKAAAIHAAPVFLDKAATTKKAISLIREAVAAGAELVAFPETYIPAFPVWAALWAP 60 Query: 61 IDNHDLFVRMAEQSVLADGPEVARLCAEARALGVTVSIGISERSAVSVGGLWNSNLLIGE 120 IDNHDLFVRMA+QSVL DGPEV + EAR LGV VSIGISE+S SVGG+WNSNLLIGE Sbjct: 61 IDNHDLFVRMADQSVLIDGPEVKAIRDEARRLGVVVSIGISEKSPASVGGIWNSNLLIGE 120 Query: 121 TGEILIHHRKLVPTFYEKLVWSSGDGAGLKVAETRQGRIGGLICGENTNPLARFALMAQG 180 GEIL HHRKLVPTFYEKL+WS GDGAGL+V +TR G+IG LICGENTNPLAR+ALMAQG Sbjct: 121 DGEILNHHRKLVPTFYEKLIWSVGDGAGLRVVDTRLGKIGQLICGENTNPLARYALMAQG 180 Query: 181 EQVHISSWPPMWPTRRPAGGGNFDNVAANRIRASAHCFEAKVFGIVTAGYMDQAMRAFLT 240 EQ HISSWPP+WPTRRPA GGN+ AA RIRASAHCFEAKVFG+VT+G +D+A R L Sbjct: 181 EQFHISSWPPVWPTRRPAEGGNYHIAAATRIRASAHCFEAKVFGLVTSGVLDKAARDMLV 240 Query: 241 ERDRTIADVIDQTPRAASFFVDPTGSVFGDGLQDEEGIAYAEIDLNRCVEPKQFHDVVGY 300 RD + A V+D TPRAA+FF+DPTG G+ L ++EGI YA+IDLNRCVEPKQFHDVVGY Sbjct: 241 ARDPSAAAVLDGTPRAATFFLDPTGEQIGEALCEDEGILYADIDLNRCVEPKQFHDVVGY 300 Query: 301 YNRFDIFGVTVDRSRLTPARFRDADLPIAQVQD 333 YNRFD+F V++ R RL+PA F D DLP+ V D Sbjct: 301 YNRFDVFAVSISRRRLSPATFID-DLPLPAVVD 332 >ref|YP_002289780.1| aliphatic nitrilase [Oligotropha carboxidovorans OM5] ref|YP_004632228.1| nitrilase [Oligotropha carboxidovorans OM5] gb|ACI93915.1| aliphatic nitrilase [Oligotropha carboxidovorans OM5] gb|AEI02411.1| nitrilase [Oligotropha carboxidovorans OM4] gb|AEI05987.1| nitrilase [Oligotropha carboxidovorans OM5] Length = 354 Score = 514 bits (1325), Expect = e-144, Method: Compositional matrix adjust. Identities = 253/350 (72%), Positives = 283/350 (80%), Gaps = 2/350 (0%) Query: 1 MLPRFKAAAVHAAPVFLDSWATTEKAIAIIREAARAGAELIAFPETYIPAFPVWAAMWPP 60 M K AAVHAAPVFLD ATT KAI+I+REAA AGAEL+AFPETYIPAFPVWAA+W P Sbjct: 1 MSSLVKVAAVHAAPVFLDRAATTAKAISILREAANAGAELVAFPETYIPAFPVWAALWAP 60 Query: 61 IDNHDLFVRMAEQSVLADGPEVARLCAEARALGVTVSIGISERSAVSVGGLWNSNLLIGE 120 IDNHDLF MAEQSV GPE+A L EARALGV VS+GISE S SVG +WNSN+LIG Sbjct: 61 IDNHDLFTLMAEQSVSLHGPEIAALRREARALGVIVSMGISESSNASVGAIWNSNVLIGS 120 Query: 121 TGEILIHHRKLVPTFYEKLVWSSGDGAGLKVAETRQGRIGGLICGENTNPLARFALMAQG 180 G+IL HHRKLVPTFYEKLVW++GDGAGLKV+ T GRIG LICGENTNPLAR+ LMAQ Sbjct: 121 NGDILNHHRKLVPTFYEKLVWAAGDGAGLKVSSTPIGRIGNLICGENTNPLARYTLMAQA 180 Query: 181 EQVHISSWPPMWPTRRPAGGGNFDNVAANRIRASAHCFEAKVFGIVTAGYMDQAMRAFLT 240 EQ+HISSWPPMWPTRRP+ GGNF+NVAANRIRASAH FEAK +GIVTAGYMD AMR FL Sbjct: 181 EQIHISSWPPMWPTRRPSTGGNFNNVAANRIRASAHSFEAKAYGIVTAGYMDAAMRDFLI 240 Query: 241 ERDRTIADVIDQTPRAASFFVDPTGSVFGDGLQDEEGIAYAEIDLNRCVEPKQFHDVVGY 300 RD ++ +VID+T RAASFFVDPTG GD LQD+EGIAYAE DL RC+EPKQFHDVVGY Sbjct: 241 ARDPSVVEVIDRTQRAASFFVDPTGEAVGDLLQDQEGIAYAEFDLTRCIEPKQFHDVVGY 300 Query: 301 YNRFDIFGVTVDRSRLTPARFRD-ADLPIAQVQDAAILAVDADAGSNGQG 349 YNRFD+F +TV+R RL PA F D A+ P A D + A+D + NG G Sbjct: 301 YNRFDVFDLTVNRKRLKPATFLDIAETPAASETDES-GALDDASFRNGNG 349 >ref|ZP_07027303.1| Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase [Afipia sp. 1NLS2] gb|EFI51059.1| Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase [Afipia sp. 1NLS2] Length = 346 Score = 473 bits (1216), Expect = e-131, Method: Compositional matrix adjust. Identities = 225/322 (69%), Positives = 259/322 (80%) Query: 2 LPRFKAAAVHAAPVFLDSWATTEKAIAIIREAARAGAELIAFPETYIPAFPVWAAMWPPI 61 +P K AAVHAAPVFLD ATT KAI+IIREAAR GA+LIAFPET+IPAFPVW+ +W P Sbjct: 1 MPHVKVAAVHAAPVFLDRTATTAKAISIIREAARGGAQLIAFPETFIPAFPVWSGLWAPT 60 Query: 62 DNHDLFVRMAEQSVLADGPEVARLCAEARALGVTVSIGISERSAVSVGGLWNSNLLIGET 121 +NHDLF MA QSV A+GPE+A L EA+ALGV VS+GISE S SVG LWNS ++IG Sbjct: 61 ENHDLFSLMANQSVSANGPEIAALRREAKALGVCVSMGISESSPASVGALWNSFIMIGPD 120 Query: 122 GEILIHHRKLVPTFYEKLVWSSGDGAGLKVAETRQGRIGGLICGENTNPLARFALMAQGE 181 G IL HHRKLVPTFYEKL W+ GDGAGLKV ET GR+G LICGENTNPLAR+AL+AQ E Sbjct: 121 GGILNHHRKLVPTFYEKLTWAPGDGAGLKVVETPVGRVGQLICGENTNPLARYALIAQAE 180 Query: 182 QVHISSWPPMWPTRRPAGGGNFDNVAANRIRASAHCFEAKVFGIVTAGYMDQAMRAFLTE 241 Q+HISSWP +WPTR G N++NV ANRIR+SAH FEAK FG+ TAGYMD AMR FL + Sbjct: 181 QIHISSWPALWPTRPAGNGTNYNNVEANRIRSSAHSFEAKAFGMNTAGYMDSAMRKFLVD 240 Query: 242 RDRTIADVIDQTPRAASFFVDPTGSVFGDGLQDEEGIAYAEIDLNRCVEPKQFHDVVGYY 301 RD +AD +D++ RAASFFVDPTG+ GD LQD+EGIAYAE DL+RCVEPKQ+HDV GYY Sbjct: 241 RDPKVADTLDRSSRAASFFVDPTGAPVGDFLQDDEGIAYAEFDLSRCVEPKQYHDVTGYY 300 Query: 302 NRFDIFGVTVDRSRLTPARFRD 323 NRFDIF +TV+R R P F D Sbjct: 301 NRFDIFSLTVNRKRQHPITFVD 322 >ref|YP_002907672.1| nitrilase [Burkholderia glumae BGR1] gb|ACR32821.1| Putative nitrilase [Burkholderia glumae BGR1] Length = 338 Score = 453 bits (1165), Expect = e-125, Method: Compositional matrix adjust. Identities = 213/321 (66%), Positives = 255/321 (79%) Query: 5 FKAAAVHAAPVFLDSWATTEKAIAIIREAARAGAELIAFPETYIPAFPVWAAMWPPIDNH 64 K AA H APVFLD AT KA+ +IREA AGAELI FPE++IPAFPVWA++W PIDNH Sbjct: 1 MKVAAAHVAPVFLDRAATVAKAVNLIREARSAGAELIVFPESFIPAFPVWASLWAPIDNH 60 Query: 65 DLFVRMAEQSVLADGPEVARLCAEARALGVTVSIGISERSAVSVGGLWNSNLLIGETGEI 124 LF R S+ ADGPE+A + AEA G+ VS+G SERS SVG LWNSNLLI + G + Sbjct: 61 QLFERFVAASIYADGPEIAEIRAEAERSGIFVSLGFSERSRASVGCLWNSNLLIDDRGAV 120 Query: 125 LIHHRKLVPTFYEKLVWSSGDGAGLKVAETRQGRIGGLICGENTNPLARFALMAQGEQVH 184 L HHRKLVPTF+EKLVW+ GDG GL+VA+TR GR+GGLICGENTNPLAR+ L+AQGEQ+H Sbjct: 121 LNHHRKLVPTFFEKLVWAPGDGHGLRVADTRIGRVGGLICGENTNPLARYTLIAQGEQIH 180 Query: 185 ISSWPPMWPTRRPAGGGNFDNVAANRIRASAHCFEAKVFGIVTAGYMDQAMRAFLTERDR 244 ISSWP +WPTRRP GGN+DNVAANRIRA AH FEAK FGI+ AGYMD MR L + D Sbjct: 181 ISSWPALWPTRRPDTGGNYDNVAANRIRAGAHSFEAKAFGIMCAGYMDTPMRDALLKVDP 240 Query: 245 TIADVIDQTPRAASFFVDPTGSVFGDGLQDEEGIAYAEIDLNRCVEPKQFHDVVGYYNRF 304 ++A+V+D +PRAA+ FVDPTG+ GD L +EGIAYAE+DL +CVEPKQFHDVVGYYNR+ Sbjct: 241 SVAEVLDGSPRAATMFVDPTGTPIGDTLSADEGIAYAEMDLAKCVEPKQFHDVVGYYNRY 300 Query: 305 DIFGVTVDRSRLTPARFRDAD 325 D+F + V+R+R P R+ DA+ Sbjct: 301 DVFSLNVNRTRQEPIRWTDAE 321