BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0027.p (283 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_774541.1| enoyl-CoA hydratase [Bradyrhizobium japonicum U... 476 e-132 ref|ZP_08628321.1| enoyl-CoA hydratase [Bradyrhizobiaceae bacter... 371 e-101 ref|ZP_01625951.1| enoyl-CoA hydratase [marine gamma proteobacte... 248 6e-64 ref|ZP_02190480.1| enoyl-CoA hydratase [alpha proteobacterium BA... 238 9e-61 ref|ZP_01616083.1| enoyl-CoA hydratase [marine gamma proteobacte... 227 1e-57 >ref|NP_774541.1| enoyl-CoA hydratase [Bradyrhizobium japonicum USDA 110] dbj|BAC53166.1| blr7901 [Bradyrhizobium japonicum USDA 110] Length = 276 Score = 476 bits (1224), Expect = e-132, Method: Compositional matrix adjust. Identities = 231/263 (87%), Positives = 248/263 (94%) Query: 20 ELLCVIRDRVAVITLNRPDARNSLSDTLTPALRTMIRTCGENPEVGVLLVTGAGKAFCAG 79 ELLCVIRDRVAVITLNRP+ARNSLSD+LTPALRTMIRTCGE+P+VG LL+TGAG+AFCAG Sbjct: 13 ELLCVIRDRVAVITLNRPEARNSLSDSLTPALRTMIRTCGESPDVGALLITGAGEAFCAG 72 Query: 80 GNVKGMGAHRDKKKLEMSYEDKVADLQERQRLLTGALASVRKPTIAALPGPAVGAGLAIA 139 GNVKGMGAHRD KKLEMS ED+VADLQERQRLLTGAL SVRKPTIAALPGPAVGAGLAIA Sbjct: 73 GNVKGMGAHRDPKKLEMSLEDRVADLQERQRLLTGALVSVRKPTIAALPGPAVGAGLAIA 132 Query: 140 MACDIRIAAQSAFISTGYLRVALSGDYGMAWLLTRLVGTSRARELMFTSEKVDAARCEQI 199 MACDIRIAAQSAF++TGY R+ALSGDYG+AWLLTRLVGT+RARELMFT ++VDAARCE I Sbjct: 133 MACDIRIAAQSAFVATGYARIALSGDYGIAWLLTRLVGTARARELMFTGDRVDAARCEAI 192 Query: 200 GLVNRVVPDETLQDEAFALAKSIAEGPTLALRYMKDNLDEALQFDFATARDHEAERLIRL 259 GLVNRVVPD+ LQ EAFALAKS+AEGP LALRYMKDNLDEA+QFDF TARDHEAERLIRL Sbjct: 193 GLVNRVVPDDKLQAEAFALAKSLAEGPRLALRYMKDNLDEAIQFDFETARDHEAERLIRL 252 Query: 260 TTTADHKEAVQAFIEKRKPVCRG 282 T TADHKEAVQAFIEKRK V G Sbjct: 253 TATADHKEAVQAFIEKRKAVFTG 275 >ref|ZP_08628321.1| enoyl-CoA hydratase [Bradyrhizobiaceae bacterium SG-6C] gb|EGP09230.1| enoyl-CoA hydratase [Bradyrhizobiaceae bacterium SG-6C] Length = 282 Score = 371 bits (953), Expect = e-101, Method: Compositional matrix adjust. Identities = 188/267 (70%), Positives = 227/267 (85%), Gaps = 1/267 (0%) Query: 14 IETGTDELLCVIRDRVAVITLNRPDARNSLSDTLTPALRTMIRTCGENPEVGVLLVTGAG 73 I+TGT+ELLC++R+RVAVITLNRP ARNSLSD LTPALR MI+ CGE+P +G LL+TGAG Sbjct: 12 IDTGTEELLCMVRERVAVITLNRPQARNSLSDNLTPALRRMIKQCGEDPTIGALLITGAG 71 Query: 74 KAFCAGGNVKGMGAHRDKKKL-EMSYEDKVADLQERQRLLTGALASVRKPTIAALPGPAV 132 AFCAGG+VKGMG E++++ KVA LQERQR LTGAL +VRKPTIAALPGPA Sbjct: 72 DAFCAGGDVKGMGGKPGSSNAPELTFDQKVARLQERQRTLTGALVNVRKPTIAALPGPAA 131 Query: 133 GAGLAIAMACDIRIAAQSAFISTGYLRVALSGDYGMAWLLTRLVGTSRARELMFTSEKVD 192 GAGL++A+ACD+RIAA+SAF++T Y+++ LSGDYGMAWLLTRLVGTSRARELMF EK+D Sbjct: 132 GAGLSLALACDLRIAAESAFVTTAYVKIGLSGDYGMAWLLTRLVGTSRARELMFFGEKID 191 Query: 193 AARCEQIGLVNRVVPDETLQDEAFALAKSIAEGPTLALRYMKDNLDEALQFDFATARDHE 252 A RCE IG+VNRVVPD+ L++EAF AK +AEGP ALR++KDNLDEAL DF T+ DHE Sbjct: 192 ARRCEAIGIVNRVVPDDKLREEAFTWAKRLAEGPATALRFLKDNLDEALMNDFLTSLDHE 251 Query: 253 AERLIRLTTTADHKEAVQAFIEKRKPV 279 AER+++ TADHKEAV+AF+EKRKPV Sbjct: 252 AERMVKSAGTADHKEAVKAFVEKRKPV 278 >ref|ZP_01625951.1| enoyl-CoA hydratase [marine gamma proteobacterium HTCC2080] gb|EAW41470.1| enoyl-CoA hydratase [marine gamma proteobacterium HTCC2080] Length = 272 Score = 248 bits (634), Expect = 6e-64, Method: Compositional matrix adjust. Identities = 129/268 (48%), Positives = 175/268 (65%), Gaps = 3/268 (1%) Query: 14 IETGTDELLCVIRDRVAVITLNRPDARNSLSDTLTPALRTMIRTCGENPEVGVLLVTGAG 73 ++TGTDELL + DRVA +TLNRP+ RN+LSD LTPALR ++ +VG +++TG+G Sbjct: 2 LDTGTDELLASLDDRVATVTLNRPEKRNALSDRLTPALRQILLDLELRDDVGCIVITGSG 61 Query: 74 KAFCAGGNVKGMGAH--RDKKKLEMSYEDKVADLQERQRLLTGALASVRKPTIAALPGPA 131 AFCAGG++ G+ + D+ K +S E +V L RQ LT L + KPTIAALPG A Sbjct: 62 SAFCAGGDISGLADNVSADQSK-ALSQEAQVRQLVHRQETLTLRLHNHAKPTIAALPGVA 120 Query: 132 VGAGLAIAMACDIRIAAQSAFISTGYLRVALSGDYGMAWLLTRLVGTSRARELMFTSEKV 191 GAG +A+ACD+RIA +SAF++T Y V SGDYG +WLL LVG + +EL FT +V Sbjct: 121 AGAGFCLALACDLRIACESAFVTTAYRNVGFSGDYGGSWLLNALVGPGKTKELYFTGRRV 180 Query: 192 DAARCEQIGLVNRVVPDETLQDEAFALAKSIAEGPTLALRYMKDNLDEALQFDFATARDH 251 + +G+ N VV D+ L LA+ IA GP +AL YMK+N++ A Q Sbjct: 181 QSNEALDLGIFNTVVADDALTSTVAELARHIAHGPAVALGYMKENINRASQAGLQECLAL 240 Query: 252 EAERLIRLTTTADHKEAVQAFIEKRKPV 279 EA+RL+R + DH+EAV AF+EKR+PV Sbjct: 241 EADRLVRCAASPDHQEAVSAFMEKRQPV 268 >ref|ZP_02190480.1| enoyl-CoA hydratase [alpha proteobacterium BAL199] gb|EDP62626.1| enoyl-CoA hydratase [alpha proteobacterium BAL199] Length = 262 Score = 238 bits (606), Expect = 9e-61, Method: Compositional matrix adjust. Identities = 136/258 (52%), Positives = 179/258 (69%), Gaps = 3/258 (1%) Query: 25 IRDRVAVITLNRPDARNSLSDTLTPALRTMIRTCGENPEVGVLLVTGAGKAFCAGGNVKG 84 IR VA +T NRP+ RN+L D +T ALR M+ +PEV V+++TGAGKAFC GG+VKG Sbjct: 7 IRSHVATLTFNRPEKRNALGDIVTLALREMLLLLEADPEVRVVVLTGAGKAFCVGGDVKG 66 Query: 85 MGAHRDKKKLEMSYEDKVADLQERQRLLTGALASVRKPTIAALPGPAVGAGLAIAMACDI 144 MG+ + S +DK+ LQ RQ+ LT L + KPTIAALPG A GAG++IA+ACD+ Sbjct: 67 MGSGAPDDR---SLDDKIRALQHRQQTLTLRLFEMPKPTIAALPGAAAGAGMSIALACDL 123 Query: 145 RIAAQSAFISTGYLRVALSGDYGMAWLLTRLVGTSRARELMFTSEKVDAARCEQIGLVNR 204 R+ A SAF + G+ + LSGDYG +W LT++VG ++A+E+ FT +V A +GL+N Sbjct: 124 RVGAASAFFAPGFGAIGLSGDYGGSWHLTQIVGPAKAKEIYFTGRRVHAEEALALGLLNE 183 Query: 205 VVPDETLQDEAFALAKSIAEGPTLALRYMKDNLDEALQFDFATARDHEAERLIRLTTTAD 264 VVPDE LQ LA IA GP LA+RYMK N++ A+Q DF T + EA+RL+R+ T D Sbjct: 184 VVPDEDLQARTAELAAQIAGGPPLAIRYMKQNINRAMQADFKTCLNWEADRLVRVAQTDD 243 Query: 265 HKEAVQAFIEKRKPVCRG 282 HKEAV+AFIEKR P RG Sbjct: 244 HKEAVRAFIEKRPPEFRG 261 >ref|ZP_01616083.1| enoyl-CoA hydratase [marine gamma proteobacterium HTCC2143] gb|EAW33166.1| enoyl-CoA hydratase [marine gamma proteobacterium HTCC2143] Length = 272 Score = 227 bits (579), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 127/269 (47%), Positives = 175/269 (65%), Gaps = 4/269 (1%) Query: 11 EIKIETGTDELLCVIRDRVAVITLNRPDARNSLSDTLTPALRTMIRTCGENPEVGVLLVT 70 I IETGT ELLC I DRV VITLN+PD +N+L D LTPALR + ++ G +L+T Sbjct: 3 NITIETGTTELLCHIEDRVGVITLNKPDKKNALGDILTPALRRTLLLLEDDNRAGCILIT 62 Query: 71 GAGKAFCAGGNVKGMGAHRDKKKLEMSYEDKVADLQERQRLLTGALASVRKPTIAALPGP 130 G+G AFC+GG+V GM + E+++ADL +Q LT L + K TIAALPG Sbjct: 63 GSGSAFCSGGDVSGMAGESKPQH----REERIADLLHKQITLTERLHQLNKVTIAALPGA 118 Query: 131 AVGAGLAIAMACDIRIAAQSAFISTGYLRVALSGDYGMAWLLTRLVGTSRARELMFTSEK 190 A GAGL+IA+ACD+RIA+ +AFI+T + + LSGDYG +W L RL+G +A+EL + S + Sbjct: 119 AAGAGLSIALACDLRIASDNAFITTAFRNIGLSGDYGTSWFLPRLIGMGKAKELFYRSAR 178 Query: 191 VDAARCEQIGLVNRVVPDETLQDEAFALAKSIAEGPTLALRYMKDNLDEALQFDFATARD 250 V+AA E++G+ N V P ++ + + A IA GPT AL MK NL+ +L + + Sbjct: 179 VNAAEAERLGIFNCVFPQQSFRQDMMTYASQIANGPTNALSRMKINLNASLDQSLSESLA 238 Query: 251 HEAERLIRLTTTADHKEAVQAFIEKRKPV 279 EA+ LI TA+ KEA+ AF++KR PV Sbjct: 239 LEAKHLIDSAGTAESKEAISAFMQKRSPV 267